Restriction map for
pF1KB6679
(Circular) (Six-base) MAPSORT of: pF1KB6679.seq Check: 114 from: 1 to: 4394
pF1KB6679 4394 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 2219 2651 3398 3666 613
Size: 1606 432 747 268 1341
Fragments arranged by size:
1606 1341 747 432 268
AflIII A'CryG_T
Cuts at: 1226 1746 2850 3827 1226
Size: 520 1104 977 1793
Fragments arranged by size:
1793 1104 977 520
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 294 1699 3266 294
Size: 1405 1567 1422
Fragments arranged by size:
1567 1422 1405
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 525 911 1303 1398 3747 4003 525
Size: 386 392 95 2349 256 916
Fragments arranged by size:
2349 916 392 386 256 95
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 202 1414 202
Size: 1212 3182
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 464 1410 1824 2072 2107 464
Size: 946 414 248 35 2751
Fragments arranged by size:
2751 946 414 248 35
BanII G_rGCy'C
Cuts at: 203 232 377 1011 1408 2438 203
Size: 29 145 634 397 1030 2159
Fragments arranged by size:
2159 1030 634 397 145 29
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 145 145
Size: 4394
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglI GCCn_nnn'nGGC
Cuts at: 969 1079 969
Size: 110 4284
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 2019 2112 3168 2019
Size: 93 1056 3245
Fragments arranged by size:
3245 1056 93
BmgBI CAC'GTC
Cuts at: 1227 1227
Size: 4394
BmrI ACTGGGnnnn_n'
Cuts at: 596 1056 1606 1687 2022 4166 596
Size: 460 550 81 335 2144 824
Fragments arranged by size:
2144 824 550 460 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1030 2761 1030
Size: 1731 2663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 217 361 593 1380 211
Size: 6 144 232 787 3225
Fragments arranged by size:
3225 787 232 144 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1570 2449 2941 3239 1570
Size: 879 492 298 2725
Fragments arranged by size:
2725 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4098 4098
Size: 4394
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1825 2073 2775 4271 1825
Size: 248 702 1496 1948
Fragments arranged by size:
1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 232 410 1583 2104 2782 3056 3203 3614 232
Size: 178 1173 521 678 274 147 411 1012
Fragments arranged by size:
1173 1012 678 521 411 274 178 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 168 198 755 785 168
Size: 30 557 30 3777
Fragments arranged by size:
3777 557 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 189 621 189
Size: 432 3962
BseYI C'CCAG_C
Cuts at: 339 478 1819 2577 3154 3650 339
Size: 139 1341 758 577 496 1083
Fragments arranged by size:
1341 1083 758 577 496 139
BsiEI CG_ry'CG
Cuts at: 104 1223 1936 1982 3190 104
Size: 1119 713 46 1208 1308
Fragments arranged by size:
1308 1208 1119 713 46
BsiHKAI G_wGCw'C
Cuts at: 1408 2186 2376 3168 1408
Size: 778 190 792 2634
Fragments arranged by size:
2634 792 778 190
BsmI GAATG_Cn'
Cuts at: 830 830
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 203 232 377 1011 1408 2019 2112 2186 2376
Size: 29 145 634 397 611 93 74 190
Cuts at: 2376 2438 3168 203
Size: 62 730 1429
Fragments arranged by size:
1429 730 634 611 397 190 145 93
74 62 29
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 929 1444 1658 1960 2341 929
Size: 515 214 302 381 2982
Fragments arranged by size:
2982 515 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1343 2306 1343
Size: 963 3431
BsrFI r'CCGG_y
Cuts at: 2392 2573 2782 2392
Size: 181 209 4004
Fragments arranged by size:
4004 209 181
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstEII G'GTnAC_C
Cuts at: 1342 1342
Size: 4394
BstXI CCAn_nnnn'nTGG
Cuts at: 486 486
Size: 4394
BstYI r'GATC_y
Cuts at: 1 290 1040 1179 1419 1886 1909 2244 2490
Size: 289 750 139 240 467 23 335 246
Cuts at: 2490 3502 3588 3600 3946 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 750 467 449 346 335 289 246
240 139 86 23 12
BtgI C'CryG_G
Cuts at: 107 485 1022 1313 107
Size: 378 537 291 3188
Fragments arranged by size:
3188 537 378 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 1637 1637
Size: 4394
DraI TTT'AAA
Cuts at: 666 1393 666
Size: 727 3667
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 355 881 1979 2153 2544 2571 3916 355
Size: 526 1098 174 391 27 1345 833
Fragments arranged by size:
1345 1098 833 526 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 1027 2417 2627 1027
Size: 1390 210 2794
Fragments arranged by size:
2794 1390 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 217 361 593 613 1380 2219 2651 3398
Size: 6 144 232 20 767 839 432 747
Cuts at: 3398 3666 211
Size: 268 939
Fragments arranged by size:
939 839 767 747 432 268 232 144
20 6
EcoNI CCTnn'n_nnAGG
Cuts at: 438 925 438
Size: 487 3907
EcoO109I rG'GnC_Cy
Cuts at: 320 918 1533 320
Size: 598 615 3181
Fragments arranged by size:
3181 615 598
EcoRI G'AATT_C
Cuts at: 1303 1398 1303
Size: 95 4299
EcoRV GAT'ATC
Cuts at: 513 513
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 1828 2076 3098 4077 1828
Size: 248 1022 979 2145
Fragments arranged by size:
2145 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 168 200 566 598 1225 1242 1257 1261 1274
Size: 32 366 32 627 17 15 4 13
Cuts at: 1274 1293 2236 2266 2268 2298 168
Size: 19 943 30 2 30 2264
Fragments arranged by size:
2264 943 627 366 32 32 30 30
19 17 15 13 4 2
HincII GTy'rAC
Cuts at: 981 1433 981
Size: 452 3942
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3065 3249 3065
Size: 184 4210
MscI TGG'CCA
Cuts at: 357 883 2155 357
Size: 526 1272 2596
Fragments arranged by size:
2596 1272 526
MslI CAynn'nnrTG
Cuts at: 1162 2383 2510 1162
Size: 1221 127 3046
Fragments arranged by size:
3046 1221 127
MspA1I CmG'CkG
Cuts at: 1505 1819 2179 3192 3437 1505
Size: 314 360 1013 245 2462
Fragments arranged by size:
2462 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 107 1313 107
Size: 1206 3188
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NspI r_CATG'y
Cuts at: 850 1447 1947 2854 850
Size: 597 500 907 2390
Fragments arranged by size:
2390 907 597 500
PasI CC'CwG_GG
Cuts at: 1077 1077
Size: 4394
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PpuMI rG'GwC_Cy
Cuts at: 320 918 320
Size: 598 3796
PstI C_TGCA'G
Cuts at: 1441 2126 1441
Size: 685 3709
PvuI CG_AT'CG
Cuts at: 104 1223 104
Size: 1119 3275
PvuII CAG'CTG
Cuts at: 1819 2179 1819
Size: 360 4034
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 1027 2417 2627 1027
Size: 1390 210 2794
Fragments arranged by size:
2794 1390 210
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 1437 2122 3115 3306 33
Size: 1404 685 993 191 1121
Fragments arranged by size:
1404 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 1549 2464 2956 3218 1549
Size: 915 492 262 2725
Fragments arranged by size:
2725 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StyI C'CwwG_G
Cuts at: 107 455 1313 1528 1882 107
Size: 348 858 215 354 2619
Fragments arranged by size:
2619 858 354 348 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 258 482 1137 1977 2110 2569 2778 258
Size: 224 655 840 133 459 209 1874
Fragments arranged by size:
1874 840 655 459 224 209 133
TatI w'GTAC_w
Cuts at: 3909 3909
Size: 4394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 755 787 3911 3943 755
Size: 32 3124 32 1206
Fragments arranged by size:
3124 1206 32 32
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 869 1889 869
Size: 1020 3374
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI