Restriction map for
pF1KB6681
(Circular) (Six-base) MAPSORT of: pF1KB6681.seq Check: 4775 from: 1 to: 4394
pF1KB6681 4394 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 861 867 888 861
Size: 6 21 4367
Fragments arranged by size:
4367 21 6
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 781 811 1211 2219 2651 3398 3666 580
Size: 201 30 400 1008 432 747 268 1308
Fragments arranged by size:
1308 1008 747 432 400 268 201 30
AfeI AGC'GCT
Cuts at: 979 979
Size: 4394
AflIII A'CryG_T
Cuts at: 1746 2850 3827 1746
Size: 1104 977 2313
Fragments arranged by size:
2313 1104 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AleI CACnn'nnGTG
Cuts at: 1246 1345 1246
Size: 99 4295
AlwNI CAG_nnn'CTG
Cuts at: 304 717 1699 3266 304
Size: 413 982 1567 1432
Fragments arranged by size:
1567 1432 982 413
ApaI G_GGCC'C
Cuts at: 894 1095 894
Size: 201 4193
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 484 1398 3747 4003 484
Size: 914 2349 256 875
Fragments arranged by size:
2349 914 875 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 950 1094 1100 1414 950
Size: 144 6 314 3930
Fragments arranged by size:
3930 314 144 6
BamHI G'GATC_C
Cuts at: 671 1419 671
Size: 748 3646
BanI G'GyrC_C
Cuts at: 1410 1824 2072 2107 1410
Size: 414 248 35 3697
Fragments arranged by size:
3697 414 248 35
BanII G_rGCy'C
Cuts at: 894 1095 1101 1408 2438 894
Size: 201 6 307 1030 2850
Fragments arranged by size:
2850 1030 307 201 6
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 385 385
Size: 4394
BbvCI CC'TCA_GC
Cuts at: 1073 1073
Size: 4394
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglI GCCn_nnn'nGGC
Cuts at: 442 1090 1129 442
Size: 648 39 3707
Fragments arranged by size:
3707 648 39
BglII A'GATC_T
Cuts at: 464 1909 3946 464
Size: 1445 2037 912
Fragments arranged by size:
2037 1445 912
BlpI GC'TnA_GC
Cuts at: 1112 1163 1506 1112
Size: 51 343 4000
Fragments arranged by size:
4000 343 51
Bme1580I G_kGCm'C
Cuts at: 894 1095 2019 2112 3168 894
Size: 201 924 93 1056 2120
Fragments arranged by size:
2120 1056 924 201 93
BmrI ACTGGGnnnn_n'
Cuts at: 163 1298 1606 1687 2022 4166 163
Size: 1135 308 81 335 2144 391
Fragments arranged by size:
2144 1135 391 335 308 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 248 2761 248
Size: 2513 1881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 430 964 1009 203
Size: 227 534 45 3588
Fragments arranged by size:
3588 534 227 45
Bpu10I CC'TnA_GC
Cuts at: 1073 1073
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 1570 2449 2941 3239 602
Size: 968 879 492 298 1757
Fragments arranged by size:
1757 968 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 150 4098 150
Size: 3948 446
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1825 2073 2775 4271 1825
Size: 248 702 1496 1948
Fragments arranged by size:
1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3056 3203 3614 1583
Size: 521 678 274 147 411 2363
Fragments arranged by size:
2363 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 176 206 176
Size: 30 4364
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 475 504 871 1219 475
Size: 29 367 348 3650
Fragments arranged by size:
3650 367 348 29
BseYI C'CCAG_C
Cuts at: 667 893 1819 2577 3154 3650 667
Size: 226 926 758 577 496 1411
Fragments arranged by size:
1411 926 758 577 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 895 928 889
Size: 6 33 4355
Fragments arranged by size:
4355 33 6
BsiEI CG_ry'CG
Cuts at: 104 356 1936 1982 3190 104
Size: 252 1580 46 1208 1308
Fragments arranged by size:
1580 1308 1208 252 46
BsiHKAI G_wGCw'C
Cuts at: 804 1408 2186 2376 3168 804
Size: 604 778 190 792 2030
Fragments arranged by size:
2030 792 778 604 190
BsmBI CGTCTCn'nnnn_
Cuts at: 406 406
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 804 894 1095 1101 1408 2019 2112 2186 2376
Size: 90 201 6 307 611 93 74 190
Cuts at: 2376 2438 3168 804
Size: 62 730 2030
Fragments arranged by size:
2030 730 611 307 201 190 93 90
74 62 6
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 861 867 888 1444 1658 1960 2341 861
Size: 6 21 556 214 302 381 2914
Fragments arranged by size:
2914 556 381 302 214 21 6
BsrBI CCG'CTC
Cuts at: 50 888 2686 2740 4224 4389 50
Size: 838 1798 54 1484 165 55
Fragments arranged by size:
1798 1484 838 165 55 54
BsrDI GCAATG_nn'
Cuts at: 308 401 2306 308
Size: 93 1905 2396
Fragments arranged by size:
2396 1905 93
BsrFI r'CCGG_y
Cuts at: 2392 2573 2782 2392
Size: 181 209 4004
Fragments arranged by size:
4004 209 181
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 867 1771 867
Size: 904 3490
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstYI r'GATC_y
Cuts at: 1 218 227 464 671 1419 1886 1909 2244
Size: 217 9 237 207 748 467 23 335
Cuts at: 2244 2490 3502 3588 3600 3946 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 748 467 449 346 335 246 237
217 207 86 23 12 9
BtgI C'CryG_G
Cuts at: 137 282 1268 137
Size: 145 986 3263
Fragments arranged by size:
3263 986 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 911 1266 1637 911
Size: 355 371 3668
Fragments arranged by size:
3668 371 355
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 784 2100 2958 784
Size: 1316 858 2220
Fragments arranged by size:
2220 1316 858
EaeI y'GGCC_r
Cuts at: 353 734 833 1121 1979 2153 2544 2571 3916
Size: 381 99 288 858 174 391 27 1345
Cuts at: 3916 353
Size: 831
Fragments arranged by size:
1345 858 831 391 381 288 174 99
27
EagI C'GGCC_G
Cuts at: 353 1979 353
Size: 1626 2768
EarI CTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1048 2602 2912 3058 4204 1048
Size: 1554 310 146 1146 1238
Fragments arranged by size:
1554 1238 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 430 580 781 811 964 1009 1211 2219
Size: 227 150 201 30 153 45 202 1008
Cuts at: 2219 2651 3398 3666 203
Size: 432 747 268 931
Fragments arranged by size:
1008 931 747 432 268 227 202 201
153 150 45 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1000 1000
Size: 4394
EcoO109I rG'GnC_Cy
Cuts at: 288 1091 1533 288
Size: 803 442 3149
Fragments arranged by size:
3149 803 442
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 981 996 1828 2076 3098 4077 981
Size: 15 832 248 1022 979 1298
Fragments arranged by size:
1298 1022 979 832 248 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 176 208 1200 1232 2236 2266 2268 2298 176
Size: 32 992 32 1004 30 2 30 2272
Fragments arranged by size:
2272 1004 992 32 32 30 30 2
HincII GTy'rAC
Cuts at: 528 789 1433 528
Size: 261 644 3489
Fragments arranged by size:
3489 644 261
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 237 3065 3249 237
Size: 2828 184 1382
Fragments arranged by size:
2828 1382 184
MscI TGG'CCA
Cuts at: 736 835 1123 2155 736
Size: 99 288 1032 2975
Fragments arranged by size:
2975 1032 288 99
MslI CAynn'nnrTG
Cuts at: 454 541 1246 1345 2383 2510 454
Size: 87 705 99 1038 127 2338
Fragments arranged by size:
2338 1038 705 127 99 87
MspA1I CmG'CkG
Cuts at: 864 967 1146 1167 1256 1505 1819 2179 3192
Size: 103 179 21 89 249 314 360 1013
Cuts at: 3192 3437 864
Size: 245 1821
Fragments arranged by size:
1821 1013 360 314 249 245 179 103
89 21
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 137 282 1268 137
Size: 145 986 3263
Fragments arranged by size:
3263 986 145
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NotI GC'GGCC_GC
Cuts at: 353 353
Size: 4394
NspI r_CATG'y
Cuts at: 1176 1447 1947 2854 1176
Size: 271 500 907 2716
Fragments arranged by size:
2716 907 500 271
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 900 900
Size: 4394
PfoI T'CCnGG_A
Cuts at: 674 674
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PpuMI rG'GwC_Cy
Cuts at: 288 288
Size: 4394
PspOMI G'GGCC_C
Cuts at: 890 1091 890
Size: 201 4193
PstI C_TGCA'G
Cuts at: 1441 2126 1441
Size: 685 3709
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 1146 1167 1819 2179 1146
Size: 21 652 360 3361
Fragments arranged by size:
3361 652 360 21
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SanDI GG'GwC_CC
Cuts at: 288 288
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 1298 3911 1298
Size: 2613 1781
SexAI A'CCwGG_T
Cuts at: 521 521
Size: 4394
SfcI C'TryA_G
Cuts at: 33 1437 2122 3115 3306 33
Size: 1404 685 993 191 1121
Fragments arranged by size:
1404 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 442 442
Size: 4394
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 952 1416 952
Size: 464 3930
SmlI C'TyrA_G
Cuts at: 617 1549 2464 2956 3218 617
Size: 932 915 492 262 1793
Fragments arranged by size:
1793 932 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StuI AGG'CCT
Cuts at: 1078 1078
Size: 4394
StyI C'CwwG_G
Cuts at: 137 282 737 1268 1528 1882 137
Size: 145 455 531 260 354 2649
Fragments arranged by size:
2649 531 455 354 260 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1977 2110 2569 2778 1977
Size: 133 459 209 3593
Fragments arranged by size:
3593 459 209 133
TatI w'GTAC_w
Cuts at: 1296 3909 1296
Size: 2613 1781
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 523 2191 523
Size: 1668 2726
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 950 1414 950
Size: 464 3930
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SacII SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI