Restriction map for
pF1KB6683
(Circular) (Six-base) MAPSORT of: pF1KB6683.seq Check: 6708 from: 1 to: 4394
pF1KB6683 4394 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1168 1168
Size: 4394
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 2219 2651 3398 3666 1393
Size: 826 432 747 268 2121
Fragments arranged by size:
2121 826 747 432 268
AflIII A'CryG_T
Cuts at: 1746 2850 3827 1746
Size: 1104 977 2313
Fragments arranged by size:
2313 1104 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 1699 3266 1699
Size: 1567 2827
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 216 614 861 964 1398 3747 4003 216
Size: 398 247 103 434 2349 256 607
Fragments arranged by size:
2349 607 434 398 256 247 103
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 271 1414 271
Size: 1143 3251
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 181 1410 1824 2072 2107 181
Size: 1229 414 248 35 2468
Fragments arranged by size:
2468 1229 414 248 35
BanII G_rGCy'C
Cuts at: 1408 2438 1408
Size: 1030 3364
BbeI G_GCGC'C
Cuts at: 185 1828 2076 185
Size: 1643 248 2503
Fragments arranged by size:
2503 1643 248
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BfrBI ATG'CAT
Cuts at: 458 509 458
Size: 51 4343
BglI GCCn_nnn'nGGC
Cuts at: 123 123
Size: 4394
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 2019 2112 3168 2019
Size: 93 1056 3245
Fragments arranged by size:
3245 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 402 1606 1687 2022 4166 402
Size: 1204 81 335 2144 630
Fragments arranged by size:
2144 1204 630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1107 1107
Size: 4394
Bpu10I CC'TnA_GC
Cuts at: 523 523
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1201 1570 2449 2941 3239 1201
Size: 369 879 492 298 2356
Fragments arranged by size:
2356 879 492 369 298
BsaI GGTCTCn'nnnn_
Cuts at: 1271 4098 1271
Size: 2827 1567
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 182 1825 2073 2775 4271 182
Size: 1643 248 702 1496 305
Fragments arranged by size:
1643 1496 702 305 248
BsaWI w'CCGG_w
Cuts at: 345 1583 2104 2782 3056 3203 3614 345
Size: 1238 521 678 274 147 411 1125
Fragments arranged by size:
1238 1125 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1819 2577 3154 3650 1819
Size: 758 577 496 2563
Fragments arranged by size:
2563 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 1282 228
Size: 1054 3340
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3190 104
Size: 1832 46 1208 1308
Fragments arranged by size:
1832 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1408 2186 2376 3168 1408
Size: 778 190 792 2634
Fragments arranged by size:
2634 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1408 2019 2112 2186 2376 2438 3168 1408
Size: 611 93 74 190 62 730 2634
Fragments arranged by size:
2634 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 345 3614 345
Size: 3269 1125
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1168 1444 1658 1960 2341 1168
Size: 276 214 302 381 3221
Fragments arranged by size:
3221 381 302 276 214
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 850 2306 850
Size: 1456 2938
BsrFI r'CCGG_y
Cuts at: 117 157 2392 2573 2782 117
Size: 40 2235 181 209 1729
Fragments arranged by size:
2235 1729 209 181 40
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstXI CCAn_nnnn'nTGG
Cuts at: 655 655
Size: 4394
BstYI r'GATC_y
Cuts at: 1 1419 1886 1909 2244 2490 3502 3588 3600
Size: 1418 467 23 335 246 1012 86 12
Cuts at: 3600 3946 1
Size: 346 449
Fragments arranged by size:
1418 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4394
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 1637 1637
Size: 4394
DraI TTT'AAA
Cuts at: 1263 1393 1263
Size: 130 4264
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 115 1979 2153 2544 2571 3916 115
Size: 1864 174 391 27 1345 593
Fragments arranged by size:
1864 1345 593 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1107 1393 2219 2651 3398 3666 1107
Size: 286 826 432 747 268 1835
Fragments arranged by size:
1835 826 747 432 286 268
EcoO109I rG'GnC_Cy
Cuts at: 404 1533 404
Size: 1129 3265
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 145 185 1828 2076 3098 4077 145
Size: 40 1643 248 1022 979 462
Fragments arranged by size:
1643 1022 979 462 248 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 249 281 375 407 469 501 973 1005 2236
Size: 32 94 32 62 32 472 32 1231
Cuts at: 2236 2266 2268 2298 249
Size: 30 2 30 2345
Fragments arranged by size:
2345 1231 472 94 62 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1433 1433
Size: 4394
KasI G'GCGC_C
Cuts at: 181 1824 2072 181
Size: 1643 248 2503
Fragments arranged by size:
2503 1643 248
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3065 3249 3065
Size: 184 4210
MscI TGG'CCA
Cuts at: 2155 2155
Size: 4394
MslI CAynn'nnrTG
Cuts at: 623 827 1147 2383 2510 623
Size: 204 320 1236 127 2507
Fragments arranged by size:
2507 1236 320 204 127
MspA1I CmG'CkG
Cuts at: 1505 1819 2179 3192 3437 1505
Size: 314 360 1013 245 2462
Fragments arranged by size:
2462 1013 360 314 245
NaeI GCC'GGC
Cuts at: 119 159 2575 119
Size: 40 2416 1938
Fragments arranged by size:
2416 1938 40
NarI GG'CG_CC
Cuts at: 182 1825 2073 182
Size: 1643 248 2503
Fragments arranged by size:
2503 1643 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4394
NgoMIV G'CCGG_C
Cuts at: 117 157 2573 117
Size: 40 2416 1938
Fragments arranged by size:
2416 1938 40
NsiI A_TGCA'T
Cuts at: 460 511 460
Size: 51 4343
NspI r_CATG'y
Cuts at: 1447 1947 2854 1447
Size: 500 907 2987
Fragments arranged by size:
2987 907 500
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 113 720 113
Size: 607 3787
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PpuMI rG'GwC_Cy
Cuts at: 404 404
Size: 4394
PstI C_TGCA'G
Cuts at: 1441 2126 1441
Size: 685 3709
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 1819 2179 1819
Size: 360 4034
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SanDI GG'GwC_CC
Cuts at: 404 404
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 1193 3911 1193
Size: 2718 1676
SfcI C'TryA_G
Cuts at: 33 1352 1437 2122 3115 3306 33
Size: 1319 85 685 993 191 1121
Fragments arranged by size:
1319 1121 993 685 191 85
SfoI GGC'GCC
Cuts at: 183 1826 2074 183
Size: 1643 248 2503
Fragments arranged by size:
2503 1643 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SgrAI Cr'CCGG_yG
Cuts at: 157 157
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 1216 1549 2464 2956 3218 1216
Size: 333 915 492 262 2392
Fragments arranged by size:
2392 915 492 333 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StyI C'CwwG_G
Cuts at: 107 1334 1528 1882 107
Size: 1227 194 354 2619
Fragments arranged by size:
2619 1227 354 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1977 2110 2569 2778 1977
Size: 133 459 209 3593
Fragments arranged by size:
3593 459 209 133
TatI w'GTAC_w
Cuts at: 1191 3909 1191
Size: 2718 1676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
XmnI GAAnn'nnTTC
Cuts at: 614 614
Size: 4394
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI ZraI