Restriction map for
pF1KB6684
(Circular) (Six-base) MAPSORT of: pF1KB6684.seq Check: 757 from: 1 to: 4481
pF1KB6684 4481 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 481 491 1085 1519 481
Size: 10 594 434 3443
Fragments arranged by size:
3443 594 434 10
Acc65I G'GTAC_C
Cuts at: 1497 1497
Size: 4481
AclI AA'CG_TT
Cuts at: 4321 4321
Size: 4481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1480 2306 2738 3485 3753 1480
Size: 826 432 747 268 2208
Fragments arranged by size:
2208 826 747 432 268
AfeI AGC'GCT
Cuts at: 718 718
Size: 4481
AflIII A'CryG_T
Cuts at: 798 1833 2937 3914 798
Size: 1035 1104 977 1365
Fragments arranged by size:
1365 1104 1035 977
AgeI A'CCGG_T
Cuts at: 2869 2869
Size: 4481
AlwNI CAG_nnn'CTG
Cuts at: 1786 3353 1786
Size: 1567 2914
ApaI G_GGCC'C
Cuts at: 615 615
Size: 4481
ApaLI G'TGCA_C
Cuts at: 898 3251 898
Size: 2353 2128
ApoI r'AATT_y
Cuts at: 924 1300 1485 3834 4090 924
Size: 376 185 2349 256 1315
Fragments arranged by size:
2349 1315 376 256 185
AseI AT'TA_AT
Cuts at: 20 20
Size: 4481
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
AvaI C'yCGr_G
Cuts at: 529 1501 529
Size: 972 3509
BamHI G'GATC_C
Cuts at: 1506 1506
Size: 4481
BanI G'GyrC_C
Cuts at: 244 1497 1911 2159 2194 244
Size: 1253 414 248 35 2531
Fragments arranged by size:
2531 1253 414 248 35
BanII G_rGCy'C
Cuts at: 615 1495 2525 615
Size: 880 1030 2571
Fragments arranged by size:
2571 1030 880
BbeI G_GCGC'C
Cuts at: 1915 2163 1915
Size: 248 4233
BbsI GAAGACnn'nnnn_
Cuts at: 1307 1307
Size: 4481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4447
BciVI GTATCCnnnnn_n'
Cuts at: 2379 3140 4481 2379
Size: 761 1341 2379
Fragments arranged by size:
2379 1341 761
BclI T'GATC_A
Cuts at: 882 2001 882
Size: 1119 3362
BfrBI ATG'CAT
Cuts at: 1122 1122
Size: 4481
BglII A'GATC_T
Cuts at: 533 1996 4033 533
Size: 1463 2037 981
Fragments arranged by size:
2037 1463 981
BlpI GC'TnA_GC
Cuts at: 163 1593 163
Size: 1430 3051
Bme1580I G_kGCm'C
Cuts at: 615 902 2106 2199 3255 615
Size: 287 1204 93 1056 1841
Fragments arranged by size:
1841 1204 1056 287 93
BmrI ACTGGGnnnn_n'
Cuts at: 1693 1774 2109 4253 1693
Size: 81 335 2144 1921
Fragments arranged by size:
2144 1921 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 754 2848 754
Size: 2094 2387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 940 749
Size: 191 4290
Bpu10I CC'TnA_GC
Cuts at: 1192 1232 1192
Size: 40 4441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1657 2536 3028 3326 1657
Size: 879 492 298 2812
Fragments arranged by size:
2812 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4185 4185
Size: 4481
BsaAI yAC'GTr
Cuts at: 1834 1834
Size: 4481
BsaBI GATnn'nnATC
Cuts at: 6 1995 4032 6
Size: 1989 2037 455
Fragments arranged by size:
2037 1989 455
BsaHI Gr'CG_yC
Cuts at: 1912 2160 2862 4358 1912
Size: 248 702 1496 2035
Fragments arranged by size:
2035 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1340 1670 2191 2869 3143 3290 3701 1340
Size: 330 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 330 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 259
Size: 4481
BseYI C'CCAG_C
Cuts at: 564 654 1906 2664 3241 3737 564
Size: 90 1252 758 577 496 1308
Fragments arranged by size:
1308 1252 758 577 496 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1350 1350
Size: 4481
BsiEI CG_ry'CG
Cuts at: 104 2023 2069 3277 104
Size: 1919 46 1208 1308
Fragments arranged by size:
1919 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 902 1110 1495 2273 2463 3255 902
Size: 208 385 778 190 792 2128
Fragments arranged by size:
2128 792 778 385 208 190
BsmI GAATG_Cn'
Cuts at: 450 450
Size: 4481
Bsp1286I G_dGCh'C
Cuts at: 615 902 1110 1495 2106 2199 2273 2463 2525
Size: 287 208 385 611 93 74 190 62
Cuts at: 2525 3255 615
Size: 730 1841
Fragments arranged by size:
1841 730 611 385 287 208 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3701 3701
Size: 4481
BspHI T'CATG_A
Cuts at: 3657 3657
Size: 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 850 1531 1745 2047 2428 850
Size: 681 214 302 381 2903
Fragments arranged by size:
2903 681 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1065 2773 2827 4311 4476 50
Size: 1015 1708 54 1484 165 55
Fragments arranged by size:
1708 1484 1015 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2393 2393
Size: 4481
BsrFI r'CCGG_y
Cuts at: 2479 2660 2869 2479
Size: 181 209 4091
Fragments arranged by size:
4091 209 181
BssSI C'ACGA_G
Cuts at: 696 1132 2752 3110 696
Size: 436 1620 358 2067
Fragments arranged by size:
2067 1620 436 358
BstAPI GCAn_nnn'nTGC
Cuts at: 445 1858 445
Size: 1413 3068
BstBI TT'CG_AA
Cuts at: 283 2842 283
Size: 2559 1922
BstEII G'GTnAC_C
Cuts at: 1182 1182
Size: 4481
BstXI CCAn_nnnn'nTGG
Cuts at: 122 334 595 122
Size: 212 261 4008
Fragments arranged by size:
4008 261 212
BstYI r'GATC_y
Cuts at: 1 533 1506 1973 1996 2331 2577 3589 3675
Size: 532 973 467 23 335 246 1012 86
Cuts at: 3675 3687 4033 1
Size: 12 346 449
Fragments arranged by size:
1012 973 532 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 372 461 372
Size: 89 4392
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2431 2612 2775 2431
Size: 181 163 4137
Fragments arranged by size:
4137 181 163
BtsI GCAGTG_nn'
Cuts at: 324 1367 1724 324
Size: 1043 357 3081
Fragments arranged by size:
3081 1043 357
DraI TTT'AAA
Cuts at: 1480 1480
Size: 4481
DrdI GACnn_nn'nnGTC
Cuts at: 811 2187 3045 811
Size: 1376 858 2247
Fragments arranged by size:
2247 1376 858
EaeI y'GGCC_r
Cuts at: 2066 2240 2631 2658 4003 2066
Size: 174 391 27 1345 2544
Fragments arranged by size:
2544 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2066 2066
Size: 4481
EarI CTCTTCn'nnn_
Cuts at: 646 707 745 1215 2504 2714 646
Size: 61 38 470 1289 210 2413
Fragments arranged by size:
2413 1289 470 210 61 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2689 2999 3145 4291 2689
Size: 310 146 1146 2879
Fragments arranged by size:
2879 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1493 1493
Size: 4481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 940 1480 2306 2738 3485 3753 749
Size: 191 540 826 432 747 268 1477
Fragments arranged by size:
1477 826 747 540 432 268 191
EcoNI CCTnn'n_nnAGG
Cuts at: 1020 1020
Size: 4481
EcoO109I rG'GnC_Cy
Cuts at: 118 147 611 1620 118
Size: 29 464 1009 2979
Fragments arranged by size:
2979 1009 464 29
EcoRI G'AATT_C
Cuts at: 924 1485 924
Size: 561 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 4481
FspI TGC'GCA
Cuts at: 2262 2262
Size: 4481
HaeII r_GCGC'y
Cuts at: 230 720 917 1915 2163 3185 4164 230
Size: 490 197 998 248 1022 979 547
Fragments arranged by size:
1022 998 979 547 490 248 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 867 899 2323 2353 2355 2385 867
Size: 32 1424 30 2 30 2963
Fragments arranged by size:
2963 1424 32 30 30 2
HincII GTy'rAC
Cuts at: 816 1520 816
Size: 704 3777
KasI G'GCGC_C
Cuts at: 1911 2159 1911
Size: 248 4233
KpnI G_GTAC'C
Cuts at: 1501 1501
Size: 4481
MluI A'CGCG_T
Cuts at: 3914 3914
Size: 4481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 150 554 774 1343 3152 3336 150
Size: 404 220 569 1809 184 1295
Fragments arranged by size:
1809 1295 569 404 220 184
MscI TGG'CCA
Cuts at: 2242 2242
Size: 4481
MslI CAynn'nnrTG
Cuts at: 2470 2597 2470
Size: 127 4354
MspA1I CmG'CkG
Cuts at: 502 654 1592 1906 2266 3279 3524 502
Size: 152 938 314 360 1013 245 1459
Fragments arranged by size:
1459 1013 938 360 314 245 152
NaeI GCC'GGC
Cuts at: 2662 2662
Size: 4481
NarI GG'CG_CC
Cuts at: 1912 2160 1912
Size: 248 4233
NcoI C'CATG_G
Cuts at: 372 372
Size: 4481
NgoMIV G'CCGG_C
Cuts at: 2660 2660
Size: 4481
NsiI A_TGCA'T
Cuts at: 1124 1124
Size: 4481
NspI r_CATG'y
Cuts at: 802 1122 1534 2034 2941 802
Size: 320 412 500 907 2342
Fragments arranged by size:
2342 907 500 412 320
PasI CC'CwG_GG
Cuts at: 122 122
Size: 4481
PciI A'CATG_T
Cuts at: 798 2937 798
Size: 2139 2342
PfoI T'CCnGG_A
Cuts at: 403 403
Size: 4481
PmeI GTTT'AAAC
Cuts at: 1480 1480
Size: 4481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4449
PpuMI rG'GwC_Cy
Cuts at: 118 118
Size: 4481
PspOMI G'GGCC_C
Cuts at: 611 611
Size: 4481
PstI C_TGCA'G
Cuts at: 1528 2213 1528
Size: 685 3796
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4481
PvuII CAG'CTG
Cuts at: 502 654 1906 2266 502
Size: 152 1252 360 2717
Fragments arranged by size:
2717 1252 360 152
RsrII CG'GwC_CG
Cuts at: 2676 2676
Size: 4481
SacI G_AGCT'C
Cuts at: 1495 1495
Size: 4481
SalI G'TCGA_C
Cuts at: 1518 1518
Size: 4481
SapI GCTCTTCn'nnn_
Cuts at: 646 707 2504 2714 646
Size: 61 1797 210 2413
Fragments arranged by size:
2413 1797 210 61
SbfI CC_TGCA'GG
Cuts at: 1528 1528
Size: 4481
ScaI AGT'ACT
Cuts at: 3998 3998
Size: 4481
SfcI C'TryA_G
Cuts at: 33 482 1524 2209 3202 3393 33
Size: 449 1042 685 993 191 1121
Fragments arranged by size:
1121 1042 993 685 449 191
SfoI GGC'GCC
Cuts at: 1913 2161 1913
Size: 248 4233
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
SmaI CCC'GGG
Cuts at: 1503 1503
Size: 4481
SmlI C'TyrA_G
Cuts at: 529 1636 2551 3043 3305 529
Size: 1107 915 492 262 1705
Fragments arranged by size:
1705 1107 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4481
SphI G_CATG'C
Cuts at: 1122 1534 2034 1122
Size: 412 500 3569
Fragments arranged by size:
3569 500 412
StuI AGG'CCT
Cuts at: 671 967 671
Size: 296 4185
StyI C'CwwG_G
Cuts at: 372 1021 1070 1615 1969 372
Size: 649 49 545 354 2884
Fragments arranged by size:
2884 649 545 354 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 556 2064 2197 2656 2865 129
Size: 427 1508 133 459 209 1745
Fragments arranged by size:
1745 1508 459 427 209 133
TatI w'GTAC_w
Cuts at: 692 3996 692
Size: 3304 1177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 4449
Tth111I GACn'n_nGTC
Cuts at: 983 2278 983
Size: 1295 3186
XbaI T'CTAG_A
Cuts at: 1512 1512
Size: 4481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 832 1976 832
Size: 1144 3337
XhoI C'TCGA_G
Cuts at: 529 529
Size: 4481
XmaI C'CCGG_G
Cuts at: 1501 1501
Size: 4481
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI