Restriction map for
pF1KB6685
(Circular) (Six-base) MAPSORT of: pF1KB6685.seq Check: 9961 from: 1 to: 4394
pF1KB6685 4394 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 722 722
Size: 4394
AccI GT'mk_AC
Cuts at: 1373 1432 1373
Size: 59 4335
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 2219 2651 3398 3666 574
Size: 1645 432 747 268 1302
Fragments arranged by size:
1645 1302 747 432 268
AflIII A'CryG_T
Cuts at: 519 1746 2850 3827 519
Size: 1227 1104 977 1086
Fragments arranged by size:
1227 1104 1086 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 225 1699 3266 225
Size: 1474 1567 1353
Fragments arranged by size:
1567 1474 1353
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 568 1032 1264 1398 3747 4003 568
Size: 464 232 134 2349 256 959
Fragments arranged by size:
2349 959 464 256 232 134
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 318 677 1414 318
Size: 359 737 3298
Fragments arranged by size:
3298 737 359
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 834 1228 1410 1824 2072 2107 834
Size: 394 182 414 248 35 3121
Fragments arranged by size:
3121 414 394 248 182 35
BanII G_rGCy'C
Cuts at: 244 710 1408 2438 244
Size: 466 698 1030 2200
Fragments arranged by size:
2200 1030 698 466
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1353 1387 90
Size: 34 1229 34 3097
Fragments arranged by size:
3097 1229 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4394
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 346 1506 346
Size: 1160 3234
Bme1580I G_kGCm'C
Cuts at: 640 839 2019 2112 3168 640
Size: 199 1180 93 1056 1866
Fragments arranged by size:
1866 1180 1056 199 93
BmgBI CAC'GTC
Cuts at: 663 1215 663
Size: 552 3842
BmrI ACTGGGnnnn_n'
Cuts at: 1606 1687 2022 4166 1606
Size: 81 335 2144 1834
Fragments arranged by size:
2144 1834 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 974 1198 247
Size: 727 224 3443
Fragments arranged by size:
3443 727 224
Bpu10I CC'TnA_GC
Cuts at: 541 541
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 433 694 768 1570 2449 2941 3239 238
Size: 195 261 74 802 879 492 298 1393
Fragments arranged by size:
1393 879 802 492 298 261 195 74
BsaI GGTCTCn'nnnn_
Cuts at: 307 4098 307
Size: 3791 603
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 719 1825 2073 2775 4271 719
Size: 1106 248 702 1496 842
Fragments arranged by size:
1496 1106 842 702 248
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3056 3203 3614 1583
Size: 521 678 274 147 411 2363
Fragments arranged by size:
2363 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 121 121
Size: 4394
BseYI C'CCAG_C
Cuts at: 247 1113 1819 2577 3154 3650 247
Size: 866 706 758 577 496 991
Fragments arranged by size:
991 866 758 706 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 466 157
Size: 309 4085
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3190 104
Size: 1832 46 1208 1308
Fragments arranged by size:
1832 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 534 710 1408 2186 2376 3168 534
Size: 176 698 778 190 792 1760
Fragments arranged by size:
1760 792 778 698 190 176
BsmBI CGTCTCn'nnnn_
Cuts at: 917 917
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 244 534 640 710 839 1408 2019 2112 2186
Size: 290 106 70 129 569 611 93 74
Cuts at: 2186 2376 2438 3168 244
Size: 190 62 730 1470
Fragments arranged by size:
1470 730 611 569 290 190 129 106
93 74 70 62
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 876 3570 876
Size: 2694 1700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 134 1444 1658 1960 2341 134
Size: 1310 214 302 381 2187
Fragments arranged by size:
2187 1310 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2306 2306
Size: 4394
BsrFI r'CCGG_y
Cuts at: 1307 2392 2573 2782 1307
Size: 1085 181 209 2919
Fragments arranged by size:
2919 1085 209 181
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 480 1771 480
Size: 1291 3103
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstYI r'GATC_y
Cuts at: 1 407 1125 1419 1886 1909 2244 2490 3502
Size: 406 718 294 467 23 335 246 1012
Cuts at: 3502 3588 3600 3946 1
Size: 86 12 346 449
Fragments arranged by size:
1012 718 467 449 406 346 335 294
246 86 23 12
BtgI C'CryG_G
Cuts at: 504 584 504
Size: 80 4314
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 534 1637 534
Size: 1103 3291
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 165 501 587 1979 2153 2544 2571 3916 165
Size: 336 86 1392 174 391 27 1345 643
Fragments arranged by size:
1392 1345 643 391 336 174 86 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 613 971 1021 2417 2627 613
Size: 358 50 1396 210 2380
Fragments arranged by size:
2380 1396 358 210 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 708 1406 708
Size: 698 3696
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 574 974 1198 2219 2651 3398 3666 247
Size: 327 400 224 1021 432 747 268 975
Fragments arranged by size:
1021 975 747 432 400 327 268 224
EcoNI CCTnn'n_nnAGG
Cuts at: 953 953
Size: 4394
EcoO109I rG'GnC_Cy
Cuts at: 383 417 1533 383
Size: 34 1116 3244
Fragments arranged by size:
3244 1116 34
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 348 738 1224 2413 348
Size: 390 486 1189 2329
Fragments arranged by size:
2329 1189 486 390
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 1828 2076 3098 4077 1828
Size: 248 1022 979 2145
Fragments arranged by size:
2145 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1117 1149 2236 2266 2268 2298 1117
Size: 32 1087 30 2 30 3213
Fragments arranged by size:
3213 1087 32 30 30 2
HincII GTy'rAC
Cuts at: 1374 1433 1374
Size: 59 4335
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 519 3827 519
Size: 3308 1086
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 123 3065 3249 123
Size: 2942 184 1268
Fragments arranged by size:
2942 1268 184
MscI TGG'CCA
Cuts at: 167 2155 167
Size: 1988 2406
MslI CAynn'nnrTG
Cuts at: 188 2383 2510 188
Size: 2195 127 2072
Fragments arranged by size:
2195 2072 127
MspA1I CmG'CkG
Cuts at: 171 345 1505 1819 2179 3192 3437 171
Size: 174 1160 314 360 1013 245 1128
Fragments arranged by size:
1160 1128 1013 360 314 245 174
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 504 584 504
Size: 80 4314
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NruI TCG'CGA
Cuts at: 1258 1258
Size: 4394
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4394
NspI r_CATG'y
Cuts at: 1447 1947 2854 1447
Size: 500 907 2987
Fragments arranged by size:
2987 907 500
PasI CC'CwG_GG
Cuts at: 378 378
Size: 4394
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 510 510
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PspXI vC'TCGA_Gb
Cuts at: 677 677
Size: 4394
PstI C_TGCA'G
Cuts at: 851 1242 1441 2126 851
Size: 391 199 685 3119
Fragments arranged by size:
3119 685 391 199
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 171 1819 2179 171
Size: 1648 360 2386
Fragments arranged by size:
2386 1648 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 710 1408 710
Size: 698 3696
SalI G'TCGA_C
Cuts at: 1372 1431 1372
Size: 59 4335
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 847 1238 1437 2122 3115 3306 33
Size: 814 391 199 685 993 191 1121
Fragments arranged by size:
1121 993 814 685 391 199 191
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 320 1416 320
Size: 1096 3298
SmlI C'TyrA_G
Cuts at: 253 412 677 709 783 1549 2464 2956 3218
Size: 159 265 32 74 766 915 492 262
Cuts at: 3218 253
Size: 1429
Fragments arranged by size:
1429 915 766 492 265 262 159 74
32
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StyI C'CwwG_G
Cuts at: 504 584 954 1208 1528 1882 504
Size: 80 370 254 320 354 3016
Fragments arranged by size:
3016 370 354 320 254 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1357 1977 2110 2569 2778 1357
Size: 620 133 459 209 2973
Fragments arranged by size:
2973 620 459 209 133
TatI w'GTAC_w
Cuts at: 3909 3909
Size: 4394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 661 2191 661
Size: 1530 2864
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 375 1212 1889 375
Size: 837 677 2880
Fragments arranged by size:
2880 837 677
XhoI C'TCGA_G
Cuts at: 677 677
Size: 4394
XmaI C'CCGG_G
Cuts at: 318 1414 318
Size: 1096 3298
XmnI GAAnn'nnTTC
Cuts at: 772 980 772
Size: 208 4186
ZraI GAC'GTC
Cuts at: 720 720
Size: 4394
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI NspI PasI PciI PflMI
PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BglI BmtI BsaXI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI PacI PfoI PmlI PpuMI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI