Restriction map for
pF1KB6688
(Circular) (Six-base) MAPSORT of: pF1KB6688.seq Check: 6917 from: 1 to: 4487
pF1KB6688 4487 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 993 993
Size: 4487
AccI GT'mk_AC
Cuts at: 1210 1525 1210
Size: 315 4172
Acc65I G'GTAC_C
Cuts at: 1503 1503
Size: 4487
AclI AA'CG_TT
Cuts at: 4327 4327
Size: 4487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 2312 2744 3491 3759 919
Size: 1393 432 747 268 1647
Fragments arranged by size:
1647 1393 747 432 268
AflIII A'CryG_T
Cuts at: 1839 2943 3920 1839
Size: 1104 977 2406
Fragments arranged by size:
2406 1104 977
AgeI A'CCGG_T
Cuts at: 1371 2875 1371
Size: 1504 2983
AhdI GACnn_n'nnGTC
Cuts at: 421 421
Size: 4487
AlwNI CAG_nnn'CTG
Cuts at: 1792 3359 1792
Size: 1567 2920
ApaLI G'TGCA_C
Cuts at: 145 3257 145
Size: 3112 1375
ApoI r'AATT_y
Cuts at: 247 744 1226 1425 1491 3840 4096 247
Size: 497 482 199 66 2349 256 638
Fragments arranged by size:
2349 638 497 482 256 199 66
AseI AT'TA_AT
Cuts at: 20 20
Size: 4487
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
AvaI C'yCGr_G
Cuts at: 1507 1507
Size: 4487
AvrII C'CTAG_G
Cuts at: 994 994
Size: 4487
BamHI G'GATC_C
Cuts at: 1512 1512
Size: 4487
BanI G'GyrC_C
Cuts at: 1000 1079 1503 1917 2165 2200 1000
Size: 79 424 414 248 35 3287
Fragments arranged by size:
3287 424 414 248 79 35
BanII G_rGCy'C
Cuts at: 193 1403 1501 2531 193
Size: 1210 98 1030 2149
Fragments arranged by size:
2149 1210 1030 98
BbeI G_GCGC'C
Cuts at: 1921 2169 1921
Size: 248 4239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 711 745 711
Size: 34 4453
BciVI GTATCCnnnnn_n'
Cuts at: 2385 3146 4487 2385
Size: 761 1341 2385
Fragments arranged by size:
2385 1341 761
BclI T'GATC_A
Cuts at: 329 1070 2007 329
Size: 741 937 2809
Fragments arranged by size:
2809 937 741
BglII A'GATC_T
Cuts at: 2002 4039 2002
Size: 2037 2450
BlpI GC'TnA_GC
Cuts at: 1599 1599
Size: 4487
Bme1580I G_kGCm'C
Cuts at: 149 490 1003 1084 2112 2205 3261 149
Size: 341 513 81 1028 93 1056 1375
Fragments arranged by size:
1375 1056 1028 513 341 93 81
BmgBI CAC'GTC
Cuts at: 725 725
Size: 4487
BmrI ACTGGGnnnn_n'
Cuts at: 187 1699 1780 2115 4259 187
Size: 1512 81 335 2144 415
Fragments arranged by size:
2144 1512 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1434 1434
Size: 4487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1663 2542 3034 3332 1663
Size: 879 492 298 2818
Fragments arranged by size:
2818 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4191 4191
Size: 4487
BsaAI yAC'GTr
Cuts at: 577 806 1840 577
Size: 229 1034 3224
Fragments arranged by size:
3224 1034 229
BsaBI GATnn'nnATC
Cuts at: 6 2001 4038 6
Size: 1995 2037 455
Fragments arranged by size:
2037 1995 455
BsaHI Gr'CG_yC
Cuts at: 990 1918 2166 2868 4364 990
Size: 928 248 702 1496 1113
Fragments arranged by size:
1496 1113 928 702 248
BsaWI w'CCGG_w
Cuts at: 1371 1676 2197 2875 3149 3296 3707 1371
Size: 305 521 678 274 147 411 2151
Fragments arranged by size:
2151 678 521 411 305 274 147
BseYI C'CCAG_C
Cuts at: 1912 2670 3247 3743 1912
Size: 758 577 496 2656
Fragments arranged by size:
2656 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 838 343
Size: 495 3992
BsiEI CG_ry'CG
Cuts at: 104 923 2029 2075 3283 104
Size: 819 1106 46 1208 1308
Fragments arranged by size:
1308 1208 1106 819 46
BsiHKAI G_wGCw'C
Cuts at: 149 734 1501 2279 2469 3261 149
Size: 585 767 778 190 792 1375
Fragments arranged by size:
1375 792 778 767 585 190
BsmI GAATG_Cn'
Cuts at: 129 132 1240 129
Size: 3 1108 3376
Fragments arranged by size:
3376 1108 3
BsmBI CGTCTCn'nnnn_
Cuts at: 981 981
Size: 4487
Bsp1286I G_dGCh'C
Cuts at: 149 193 490 734 1003 1084 1403 1501 2112
Size: 44 297 244 269 81 319 98 611
Cuts at: 2112 2205 2279 2469 2531 3261 149
Size: 93 74 190 62 730 1375
Fragments arranged by size:
1375 730 611 319 297 269 244 190
98 93 81 74 62 44
BspEI T'CCGG_A
Cuts at: 3707 3707
Size: 4487
BspHI T'CATG_A
Cuts at: 302 623 3663 302
Size: 321 3040 1126
Fragments arranged by size:
3040 1126 321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1537 1751 2053 2434 1537
Size: 214 302 381 3590
Fragments arranged by size:
3590 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2779 2833 4317 4482 50
Size: 2729 54 1484 165 55
Fragments arranged by size:
2729 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 395 2399 395
Size: 2004 2483
BsrFI r'CCGG_y
Cuts at: 1371 2485 2666 2875 1371
Size: 1114 181 209 2983
Fragments arranged by size:
2983 1114 209 181
BssSI C'ACGA_G
Cuts at: 2758 3116 2758
Size: 358 4129
BstAPI GCAn_nnn'nTGC
Cuts at: 1864 1864
Size: 4487
BstBI TT'CG_AA
Cuts at: 1036 2848 1036
Size: 1812 2675
BstYI r'GATC_y
Cuts at: 1 1512 1979 2002 2337 2583 3595 3681 3693
Size: 1511 467 23 335 246 1012 86 12
Cuts at: 3693 4039 1
Size: 346 449
Fragments arranged by size:
1511 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1211 1211
Size: 4487
Bsu36I CC'TnA_GG
Cuts at: 118 118
Size: 4487
BtgI C'CryG_G
Cuts at: 107 107
Size: 4487
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2437 2618 2781 2437
Size: 181 163 4143
Fragments arranged by size:
4143 181 163
BtsI GCAGTG_nn'
Cuts at: 1316 1482 1730 1316
Size: 166 248 4073
Fragments arranged by size:
4073 248 166
DraI TTT'AAA
Cuts at: 1486 1486
Size: 4487
DraIII CAC_nnn'GTG
Cuts at: 725 725
Size: 4487
DrdI GACnn_nn'nnGTC
Cuts at: 2193 3051 2193
Size: 858 3629
EaeI y'GGCC_r
Cuts at: 271 466 858 920 2072 2246 2637 2664 4009
Size: 195 392 62 1152 174 391 27 1345
Cuts at: 4009 271
Size: 749
Fragments arranged by size:
1345 1152 749 392 391 195 174 62
27
EagI C'GGCC_G
Cuts at: 920 2072 920
Size: 1152 3335
EarI CTCTTCn'nnn_
Cuts at: 923 2510 2720 923
Size: 1587 210 2690
Fragments arranged by size:
2690 1587 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2695 3005 3151 4297 2695
Size: 310 146 1146 2885
Fragments arranged by size:
2885 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1499 1499
Size: 4487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1434 2312 2744 3491 3759 919
Size: 515 878 432 747 268 1647
Fragments arranged by size:
1647 878 747 515 432 268
EcoO109I rG'GnC_Cy
Cuts at: 761 1626 761
Size: 865 3622
EcoRI G'AATT_C
Cuts at: 1491 1491
Size: 4487
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1049 2506 1049
Size: 1457 3030
FspI TGC'GCA
Cuts at: 2268 2268
Size: 4487
HaeII r_GCGC'y
Cuts at: 1921 2169 3191 4170 1921
Size: 248 1022 979 2238
Fragments arranged by size:
2238 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 407 439 2329 2359 2361 2391 407
Size: 32 1890 30 2 30 2503
Fragments arranged by size:
2503 1890 32 30 30 2
HincII GTy'rAC
Cuts at: 1347 1526 1347
Size: 179 4308
HindIII A'AGCT_T
Cuts at: 839 1031 1150 839
Size: 192 119 4176
Fragments arranged by size:
4176 192 119
HpaI GTT'AAC
Cuts at: 1347 1347
Size: 4487
KasI G'GCGC_C
Cuts at: 1917 2165 1917
Size: 248 4239
KpnI G_GTAC'C
Cuts at: 1507 1507
Size: 4487
MluI A'CGCG_T
Cuts at: 3920 3920
Size: 4487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1271 3158 3342 1271
Size: 1887 184 2416
Fragments arranged by size:
2416 1887 184
MscI TGG'CCA
Cuts at: 273 468 2248 273
Size: 195 1780 2512
Fragments arranged by size:
2512 1780 195
MslI CAynn'nnrTG
Cuts at: 325 676 1364 2476 2603 325
Size: 351 688 1112 127 2209
Fragments arranged by size:
2209 1112 688 351 127
MspA1I CmG'CkG
Cuts at: 786 1598 1912 2272 3285 3530 786
Size: 812 314 360 1013 245 1743
Fragments arranged by size:
1743 1013 812 360 314 245
NaeI GCC'GGC
Cuts at: 2668 2668
Size: 4487
NarI GG'CG_CC
Cuts at: 1918 2166 1918
Size: 248 4239
NcoI C'CATG_G
Cuts at: 107 107
Size: 4487
NgoMIV G'CCGG_C
Cuts at: 2666 2666
Size: 4487
NspI r_CATG'y
Cuts at: 1540 2040 2947 1540
Size: 500 907 3080
Fragments arranged by size:
3080 907 500
PciI A'CATG_T
Cuts at: 2943 2943
Size: 4487
PmeI GTTT'AAAC
Cuts at: 1486 1486
Size: 4487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4455
PpuMI rG'GwC_Cy
Cuts at: 761 761
Size: 4487
PsiI TTA'TAA
Cuts at: 1268 1268
Size: 4487
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1201 1233 1201
Size: 32 4455
PstI C_TGCA'G
Cuts at: 1534 2219 1534
Size: 685 3802
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4487
PvuII CAG'CTG
Cuts at: 1912 2272 1912
Size: 360 4127
RsrII CG'GwC_CG
Cuts at: 2682 2682
Size: 4487
SacI G_AGCT'C
Cuts at: 1501 1501
Size: 4487
SalI G'TCGA_C
Cuts at: 1524 1524
Size: 4487
SapI GCTCTTCn'nnn_
Cuts at: 2510 2720 2510
Size: 210 4277
SbfI CC_TGCA'GG
Cuts at: 1534 1534
Size: 4487
ScaI AGT'ACT
Cuts at: 4004 4004
Size: 4487
SfcI C'TryA_G
Cuts at: 33 203 653 1530 2215 3208 3399 33
Size: 170 450 877 685 993 191 1121
Fragments arranged by size:
1121 993 877 685 450 191 170
SfoI GGC'GCC
Cuts at: 1919 2167 1919
Size: 248 4239
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
SmaI CCC'GGG
Cuts at: 1509 1509
Size: 4487
SmlI C'TyrA_G
Cuts at: 1642 2557 3049 3311 1642
Size: 915 492 262 2818
Fragments arranged by size:
2818 915 492 262
SnaBI TAC'GTA
Cuts at: 577 806 577
Size: 229 4258
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4487
SphI G_CATG'C
Cuts at: 1540 2040 1540
Size: 500 3987
SspI AAT'ATT
Cuts at: 1101 1101
Size: 4487
StyI C'CwwG_G
Cuts at: 107 994 1044 1621 1975 107
Size: 887 50 577 354 2619
Fragments arranged by size:
2619 887 577 354 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2070 2203 2662 2871 2070
Size: 133 459 209 3686
Fragments arranged by size:
3686 459 209 133
TatI w'GTAC_w
Cuts at: 4002 4002
Size: 4487
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1461 1493 4004 4036 1461
Size: 32 2511 32 1912
Fragments arranged by size:
2511 1912 32 32
Tth111I GACn'n_nGTC
Cuts at: 884 2284 884
Size: 1400 3087
XbaI T'CTAG_A
Cuts at: 1518 1518
Size: 4487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1982 1982
Size: 4487
XmaI C'CCGG_G
Cuts at: 1507 1507
Size: 4487
XmnI GAAnn'nnTTC
Cuts at: 1032 1151 1032
Size: 119 4368
ZraI GAC'GTC
Cuts at: 991 991
Size: 4487
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I BsaXI
BseRI BsiWI BsrGI BssHII BstEII BstXI ClaI CspCI
EcoNI EcoRV FseI FspAI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SrfI
StuI SwaI XhoI