Restriction map for
pF1KB6689
(Circular) (Six-base) MAPSORT of: pF1KB6689.seq Check: 9345 from: 1 to: 4118
pF1KB6689 4118 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 1092 1092
Size: 4118
AccI GT'mk_AC
Cuts at: 359 1156 359
Size: 797 3321
Acc65I G'GTAC_C
Cuts at: 1134 1134
Size: 4118
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 1061 1943 2375 3122 3390 559
Size: 502 882 432 747 268 1287
Fragments arranged by size:
1287 882 747 502 432 268
AflIII A'CryG_T
Cuts at: 1470 2574 3551 1470
Size: 1104 977 2037
Fragments arranged by size:
2037 1104 977
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AlwNI CAG_nnn'CTG
Cuts at: 1030 1423 2990 1030
Size: 393 1567 2158
Fragments arranged by size:
2158 1567 393
ApaI G_GGCC'C
Cuts at: 116 116
Size: 4118
ApaLI G'TGCA_C
Cuts at: 2888 2888
Size: 4118
ApoI r'AATT_y
Cuts at: 1122 3471 3727 1122
Size: 2349 256 1513
Fragments arranged by size:
2349 1513 256
AseI AT'TA_AT
Cuts at: 20 932 20
Size: 912 3206
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 446 1138 446
Size: 692 3426
BamHI G'GATC_C
Cuts at: 460 707 1143 460
Size: 247 436 3435
Fragments arranged by size:
3435 436 247
BanI G'GyrC_C
Cuts at: 1134 1548 1796 1831 1134
Size: 414 248 35 3421
Fragments arranged by size:
3421 414 248 35
BanII G_rGCy'C
Cuts at: 116 1001 1132 2162 116
Size: 885 131 1030 2072
Fragments arranged by size:
2072 1030 885 131
BbeI G_GCGC'C
Cuts at: 1552 1800 1552
Size: 248 3870
BbsI GAAGACnn'nnnn_
Cuts at: 458 1084 458
Size: 626 3492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1051 1085 1051
Size: 34 4084
BciVI GTATCCnnnnn_n'
Cuts at: 2016 2777 4118 2016
Size: 761 1341 2016
Fragments arranged by size:
2016 1341 761
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BfrBI ATG'CAT
Cuts at: 421 421
Size: 4118
BglII A'GATC_T
Cuts at: 1633 3670 1633
Size: 2037 2081
BlpI GC'TnA_GC
Cuts at: 1230 1230
Size: 4118
Bme1580I G_kGCm'C
Cuts at: 116 1743 1836 2892 116
Size: 1627 93 1056 1342
Fragments arranged by size:
1627 1342 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 716 790 1330 1411 1746 3890 716
Size: 74 540 81 335 2144 944
Fragments arranged by size:
2144 944 540 335 81 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 892 2485 892
Size: 1593 2525
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 1294 2173 2665 2963 194
Size: 1100 879 492 298 1349
Fragments arranged by size:
1349 1100 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3822 3822
Size: 4118
BsaAI yAC'GTr
Cuts at: 1471 1471
Size: 4118
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 1089 1549 1797 2499 3995 1089
Size: 460 248 702 1496 1212
Fragments arranged by size:
1496 1212 702 460 248
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2780 2927 3338 1307
Size: 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 518 518
Size: 4118
BseYI C'CCAG_C
Cuts at: 832 1543 2301 2878 3374 832
Size: 711 758 577 496 1576
Fragments arranged by size:
1576 758 711 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 117
Size: 4118
BsiEI CG_ry'CG
Cuts at: 104 1660 1706 2914 104
Size: 1556 46 1208 1308
Fragments arranged by size:
1556 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1132 1910 2100 2892 1132
Size: 778 190 792 2358
Fragments arranged by size:
2358 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 116 1001 1132 1743 1836 1910 2100 2162 2892
Size: 885 131 611 93 74 190 62 730
Cuts at: 2892 116
Size: 1342
Fragments arranged by size:
1342 885 730 611 190 131 93 74
62
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 3294 3294
Size: 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 203 1168 1382 1684 2065 203
Size: 965 214 302 381 2256
Fragments arranged by size:
2256 965 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2030 2030
Size: 4118
BsrFI r'CCGG_y
Cuts at: 909 2116 2297 2506 909
Size: 1207 181 209 2521
Fragments arranged by size:
2521 1207 209 181
BssSI C'ACGA_G
Cuts at: 729 2389 2747 729
Size: 1660 358 2100
Fragments arranged by size:
2100 1660 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstYI r'GATC_y
Cuts at: 1 460 707 1143 1610 1633 1968 2214 3226
Size: 459 247 436 467 23 335 246 1012
Cuts at: 3226 3312 3324 3670 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 459 449 436 346 335 247
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 2249 2412 2068
Size: 181 163 3774
Fragments arranged by size:
3774 181 163
BtsI GCAGTG_nn'
Cuts at: 203 1361 203
Size: 1158 2960
DraI TTT'AAA
Cuts at: 182 1117 182
Size: 935 3183
DrdI GACnn_nn'nnGTC
Cuts at: 1824 2682 1824
Size: 858 3260
EaeI y'GGCC_r
Cuts at: 1703 1877 2268 2295 3640 1703
Size: 174 391 27 1345 2181
Fragments arranged by size:
2181 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 2636 2782 3928 2326
Size: 310 146 1146 2516
Fragments arranged by size:
2516 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 1061 1943 2375 3122 3390 559
Size: 502 882 432 747 268 1287
Fragments arranged by size:
1287 882 747 502 432 268
EcoO109I rG'GnC_Cy
Cuts at: 112 113 159 745 1257 112
Size: 1 46 586 512 2973
Fragments arranged by size:
2973 586 512 46 1
EcoRI G'AATT_C
Cuts at: 1122 1122
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 615 2137 615
Size: 1522 2596
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 1552 1800 2822 3801 1552
Size: 248 1022 979 1869
Fragments arranged by size:
1869 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 445 477 947 979 1960 1990 1992 2022 445
Size: 32 470 32 981 30 2 30 2541
Fragments arranged by size:
2541 981 470 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1157 1157
Size: 4118
KasI G'GCGC_C
Cuts at: 1548 1796 1548
Size: 248 3870
KpnI G_GTAC'C
Cuts at: 1138 1138
Size: 4118
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2789 2973 2789
Size: 184 3934
MscI TGG'CCA
Cuts at: 1879 1879
Size: 4118
MslI CAynn'nnrTG
Cuts at: 2107 2234 2107
Size: 127 3991
MspA1I CmG'CkG
Cuts at: 1229 1543 1903 2916 3161 1229
Size: 314 360 1013 245 2186
Fragments arranged by size:
2186 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 4118
NarI GG'CG_CC
Cuts at: 1549 1797 1549
Size: 248 3870
NcoI C'CATG_G
Cuts at: 107 107
Size: 4118
NdeI CA'TA_TG
Cuts at: 351 351
Size: 4118
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 4118
NsiI A_TGCA'T
Cuts at: 423 423
Size: 4118
NspI r_CATG'y
Cuts at: 786 1171 1671 2578 786
Size: 385 500 907 2326
Fragments arranged by size:
2326 907 500 385
PasI CC'CwG_GG
Cuts at: 581 680 581
Size: 99 4019
PciI A'CATG_T
Cuts at: 2574 2574
Size: 4118
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 4086
PpuMI rG'GwC_Cy
Cuts at: 159 745 159
Size: 586 3532
PspOMI G'GGCC_C
Cuts at: 112 112
Size: 4118
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 622 654 622
Size: 32 4086
PstI C_TGCA'G
Cuts at: 1165 1850 1165
Size: 685 3433
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 1543 1903 1543
Size: 360 3758
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SfcI C'TryA_G
Cuts at: 33 1161 1846 2839 3030 33
Size: 1128 685 993 191 1121
Fragments arranged by size:
1128 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1550 1798 1550
Size: 248 3870
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 448 1140 448
Size: 692 3426
SmlI C'TyrA_G
Cuts at: 209 1273 2188 2680 2942 209
Size: 1064 915 492 262 1385
Fragments arranged by size:
1385 1064 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 786 1171 1671 786
Size: 385 500 3233
Fragments arranged by size:
3233 500 385
StyI C'CwwG_G
Cuts at: 107 1252 1606 107
Size: 1145 354 2619
Fragments arranged by size:
2619 1145 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 249 785 1701 1834 2293 2502 249
Size: 536 916 133 459 209 1865
Fragments arranged by size:
1865 916 536 459 209 133
TatI w'GTAC_w
Cuts at: 3633 3633
Size: 4118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4086
Tth111I GACn'n_nGTC
Cuts at: 796 1915 796
Size: 1119 2999
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1613 1613
Size: 4118
XmaI C'CCGG_G
Cuts at: 446 1138 446
Size: 692 3426
ZraI GAC'GTC
Cuts at: 1090 1090
Size: 4118
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI BpmI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI PacI
PflMI PfoI PmlI PshAI PsiI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI