Restriction map for
pF1KB6693
(Circular) (Six-base) MAPSORT of: pF1KB6693.seq Check: 2366 from: 1 to: 4400
pF1KB6693 4400 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 139 408 139
Size: 269 4131
AccI GT'mk_AC
Cuts at: 1438 1438
Size: 4400
Acc65I G'GTAC_C
Cuts at: 1416 1416
Size: 4400
AclI AA'CG_TT
Cuts at: 734 4240 734
Size: 3506 894
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 325 584 1276 1345 2225 2657 3404 3672
Size: 45 259 692 69 880 432 747 268
Cuts at: 3672 280
Size: 1008
Fragments arranged by size:
1008 880 747 692 432 268 259 69
45
AflIII A'CryG_T
Cuts at: 224 362 534 840 1752 2856 3833 224
Size: 138 172 306 912 1104 977 791
Fragments arranged by size:
1104 977 912 791 306 172 138
AgeI A'CCGG_T
Cuts at: 2788 2788
Size: 4400
AleI CACnn'nnGTG
Cuts at: 923 923
Size: 4400
AlwNI CAG_nnn'CTG
Cuts at: 814 1705 3272 814
Size: 891 1567 1942
Fragments arranged by size:
1942 1567 891
ApaI G_GGCC'C
Cuts at: 209 209
Size: 4400
ApaLI G'TGCA_C
Cuts at: 3170 3170
Size: 4400
ApoI r'AATT_y
Cuts at: 685 1044 1404 3753 4009 685
Size: 359 360 2349 256 1076
Fragments arranged by size:
2349 1076 360 359 256
AseI AT'TA_AT
Cuts at: 20 392 20
Size: 372 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
AvaI C'yCGr_G
Cuts at: 1420 1420
Size: 4400
AvrII C'CTAG_G
Cuts at: 160 160
Size: 4400
BamHI G'GATC_C
Cuts at: 1425 1425
Size: 4400
BanI G'GyrC_C
Cuts at: 156 1416 1830 2078 2113 156
Size: 1260 414 248 35 2443
Fragments arranged by size:
2443 1260 414 248 35
BanII G_rGCy'C
Cuts at: 209 1414 2444 209
Size: 1205 1030 2165
Fragments arranged by size:
2165 1205 1030
BbeI G_GCGC'C
Cuts at: 1834 2082 1834
Size: 248 4152
BciVI GTATCCnnnnn_n'
Cuts at: 2298 3059 4400 2298
Size: 761 1341 2298
Fragments arranged by size:
2298 1341 761
BclI T'GATC_A
Cuts at: 1920 1920
Size: 4400
BfrBI ATG'CAT
Cuts at: 1356 1356
Size: 4400
BglII A'GATC_T
Cuts at: 1915 3952 1915
Size: 2037 2363
BlpI GC'TnA_GC
Cuts at: 1512 1512
Size: 4400
Bme1580I G_kGCm'C
Cuts at: 209 2025 2118 3174 209
Size: 1816 93 1056 1435
Fragments arranged by size:
1816 1435 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 607 1612 1693 2028 4172 607
Size: 1005 81 335 2144 835
Fragments arranged by size:
2144 1005 835 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2767 2767
Size: 4400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 1055 1060 1576 2455 2947 3245 407
Size: 648 5 516 879 492 298 1562
Fragments arranged by size:
1562 879 648 516 492 298 5
BsaI GGTCTCn'nnnn_
Cuts at: 943 4104 943
Size: 3161 1239
BsaAI yAC'GTr
Cuts at: 1753 1753
Size: 4400
BsaBI GATnn'nnATC
Cuts at: 6 1914 3951 6
Size: 1908 2037 455
Fragments arranged by size:
2037 1908 455
BsaHI Gr'CG_yC
Cuts at: 136 405 1831 2079 2781 4277 136
Size: 269 1426 248 702 1496 259
Fragments arranged by size:
1496 1426 702 269 259 248
BsaWI w'CCGG_w
Cuts at: 290 1589 2110 2788 3062 3209 3620 290
Size: 1299 521 678 274 147 411 1070
Fragments arranged by size:
1299 1070 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 521 551 521
Size: 30 4370
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 292 295 1132 1133 292
Size: 3 837 1 3559
Fragments arranged by size:
3559 837 3 1
BseYI C'CCAG_C
Cuts at: 1076 1825 2583 3160 3656 1076
Size: 749 758 577 496 1820
Fragments arranged by size:
1820 758 749 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 1085
Size: 4400
BsiEI CG_ry'CG
Cuts at: 104 1942 1988 3196 104
Size: 1838 46 1208 1308
Fragments arranged by size:
1838 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1221 1414 2192 2382 3174 1221
Size: 193 778 190 792 2447
Fragments arranged by size:
2447 792 778 193 190
Bsp1286I G_dGCh'C
Cuts at: 209 1221 1414 2025 2118 2192 2382 2444 3174
Size: 1012 193 611 93 74 190 62 730
Cuts at: 3174 209
Size: 1435
Fragments arranged by size:
1435 1012 730 611 193 190 93 74
62
BspEI T'CCGG_A
Cuts at: 290 3620 290
Size: 3330 1070
BspHI T'CATG_A
Cuts at: 3576 3576
Size: 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1450 1664 1966 2347 1450
Size: 214 302 381 3503
Fragments arranged by size:
3503 381 302 214
BsrBI CCG'CTC
Cuts at: 50 201 2692 2746 4230 4395 50
Size: 151 2491 54 1484 165 55
Fragments arranged by size:
2491 1484 165 151 55 54
BsrDI GCAATG_nn'
Cuts at: 2312 2312
Size: 4400
BsrFI r'CCGG_y
Cuts at: 2398 2579 2788 2398
Size: 181 209 4010
Fragments arranged by size:
4010 209 181
BssSI C'ACGA_G
Cuts at: 2671 3029 2671
Size: 358 4042
BstAPI GCAn_nnn'nTGC
Cuts at: 1777 1777
Size: 4400
BstBI TT'CG_AA
Cuts at: 2761 2761
Size: 4400
BstYI r'GATC_y
Cuts at: 1 1425 1892 1915 2250 2496 3508 3594 3606
Size: 1424 467 23 335 246 1012 86 12
Cuts at: 3606 3952 1
Size: 346 449
Fragments arranged by size:
1424 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 168 168
Size: 4400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2350 2531 2694 2350
Size: 181 163 4056
Fragments arranged by size:
4056 181 163
BtsI GCAGTG_nn'
Cuts at: 331 1643 331
Size: 1312 3088
DraI TTT'AAA
Cuts at: 740 1399 740
Size: 659 3741
DrdI GACnn_nn'nnGTC
Cuts at: 2106 2964 2106
Size: 858 3542
EaeI y'GGCC_r
Cuts at: 122 1985 2159 2550 2577 3922 122
Size: 1863 174 391 27 1345 600
Fragments arranged by size:
1863 1345 600 391 174 27
EagI C'GGCC_G
Cuts at: 1985 1985
Size: 4400
EarI CTCTTCn'nnn_
Cuts at: 289 692 2423 2633 289
Size: 403 1731 210 2056
Fragments arranged by size:
2056 1731 403 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2608 2918 3064 4210 2608
Size: 310 146 1146 2798
Fragments arranged by size:
2798 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1412 1412
Size: 4400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 325 584 1276 1345 2225 2657 3404 3672
Size: 45 259 692 69 880 432 747 268
Cuts at: 3672 280
Size: 1008
Fragments arranged by size:
1008 880 747 692 432 268 259 69
45
EcoNI CCTnn'n_nnAGG
Cuts at: 597 1147 597
Size: 550 3850
EcoO109I rG'GnC_Cy
Cuts at: 164 205 309 1539 164
Size: 41 104 1230 3025
Fragments arranged by size:
3025 1230 104 41
EcoRI G'AATT_C
Cuts at: 1404 1404
Size: 4400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4400
FspI TGC'GCA
Cuts at: 2181 2181
Size: 4400
HaeII r_GCGC'y
Cuts at: 1834 2082 3104 4083 1834
Size: 248 1022 979 2151
Fragments arranged by size:
2151 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 460 492 2242 2272 2274 2304 102
Size: 32 326 32 1750 30 2 30 2198
Fragments arranged by size:
2198 1750 326 32 32 30 30 2
HincII GTy'rAC
Cuts at: 903 1439 903
Size: 536 3864
KasI G'GCGC_C
Cuts at: 1830 2078 1830
Size: 248 4152
KpnI G_GTAC'C
Cuts at: 1420 1420
Size: 4400
MluI A'CGCG_T
Cuts at: 3833 3833
Size: 4400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1361 3071 3255 1361
Size: 1710 184 2506
Fragments arranged by size:
2506 1710 184
MscI TGG'CCA
Cuts at: 2161 2161
Size: 4400
MslI CAynn'nnrTG
Cuts at: 229 923 2389 2516 229
Size: 694 1466 127 2113
Fragments arranged by size:
2113 1466 694 127
MspA1I CmG'CkG
Cuts at: 1080 1511 1825 2185 3198 3443 1080
Size: 431 314 360 1013 245 2037
Fragments arranged by size:
2037 1013 431 360 314 245
NaeI GCC'GGC
Cuts at: 2581 2581
Size: 4400
NarI GG'CG_CC
Cuts at: 1831 2079 1831
Size: 248 4152
NgoMIV G'CCGG_C
Cuts at: 2579 2579
Size: 4400
NsiI A_TGCA'T
Cuts at: 1358 1358
Size: 4400
NspI r_CATG'y
Cuts at: 228 366 538 1356 1360 1453 1953 2860 228
Size: 138 172 818 4 93 500 907 1768
Fragments arranged by size:
1768 907 818 500 172 138 93 4
PciI A'CATG_T
Cuts at: 224 362 534 2856 224
Size: 138 172 2322 1768
Fragments arranged by size:
2322 1768 172 138
PmeI GTTT'AAAC
Cuts at: 1399 1399
Size: 4400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 268 300 3539 3571 268
Size: 32 3239 32 1097
Fragments arranged by size:
3239 1097 32 32
PpuMI rG'GwC_Cy
Cuts at: 164 164
Size: 4400
PspOMI G'GGCC_C
Cuts at: 205 205
Size: 4400
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 530 562 830 862 1292 1324 530
Size: 32 268 32 430 32 3606
Fragments arranged by size:
3606 430 268 32 32 32
PstI C_TGCA'G
Cuts at: 495 819 1447 2132 495
Size: 324 628 685 2763
Fragments arranged by size:
2763 685 628 324
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4400
PvuII CAG'CTG
Cuts at: 1080 1825 2185 1080
Size: 745 360 3295
Fragments arranged by size:
3295 745 360
RsrII CG'GwC_CG
Cuts at: 2595 2595
Size: 4400
SacI G_AGCT'C
Cuts at: 1414 1414
Size: 4400
SalI G'TCGA_C
Cuts at: 1437 1437
Size: 4400
SapI GCTCTTCn'nnn_
Cuts at: 289 2423 2633 289
Size: 2134 210 2056
Fragments arranged by size:
2134 2056 210
SbfI CC_TGCA'GG
Cuts at: 1447 1447
Size: 4400
ScaI AGT'ACT
Cuts at: 3917 3917
Size: 4400
SfcI C'TryA_G
Cuts at: 33 491 815 1268 1443 2128 3121 3312 33
Size: 458 324 453 175 685 993 191 1121
Fragments arranged by size:
1121 993 685 458 453 324 191 175
SfoI GGC'GCC
Cuts at: 1832 2080 1832
Size: 248 4152
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
SmaI CCC'GGG
Cuts at: 1422 1422
Size: 4400
SmlI C'TyrA_G
Cuts at: 422 1039 1070 1555 2470 2962 3224 422
Size: 617 31 485 915 492 262 1598
Fragments arranged by size:
1598 915 617 492 485 262 31
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4400
SphI G_CATG'C
Cuts at: 1360 1453 1953 1360
Size: 93 500 3807
Fragments arranged by size:
3807 500 93
StyI C'CwwG_G
Cuts at: 160 953 1534 1888 160
Size: 793 581 354 2672
Fragments arranged by size:
2672 793 581 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 464 1983 2116 2575 2784 464
Size: 1519 133 459 209 2080
Fragments arranged by size:
2080 1519 459 209 133
TatI w'GTAC_w
Cuts at: 370 843 1022 3915 370
Size: 473 179 2893 855
Fragments arranged by size:
2893 855 473 179
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1353 1385 3917 3949 1353
Size: 32 2532 32 1804
Fragments arranged by size:
2532 1804 32 32
Tth111I GACn'n_nGTC
Cuts at: 2197 2197
Size: 4400
XbaI T'CTAG_A
Cuts at: 1431 1431
Size: 4400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 922 960 1111 1895 922
Size: 38 151 784 3427
Fragments arranged by size:
3427 784 151 38
XmaI C'CCGG_G
Cuts at: 1420 1420
Size: 4400
ZraI GAC'GTC
Cuts at: 137 406 137
Size: 269 4131
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PmeI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI AscI BaeI
BbsI BbvCI BcgI BglI BmgBI BmtI BpmI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI