Restriction map for
pF1KB6694
(Circular) (Six-base) MAPSORT of: pF1KB6694.seq Check: 7435 from: 1 to: 4499
pF1KB6694 4499 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 866 1537 866
Size: 671 3828
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 959 1079 4339 959
Size: 120 3260 1119
Fragments arranged by size:
3260 1119 120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 2324 2756 3503 3771 1252
Size: 1072 432 747 268 1980
Fragments arranged by size:
1980 1072 747 432 268
AflIII A'CryG_T
Cuts at: 150 1851 2955 3932 150
Size: 1701 1104 977 717
Fragments arranged by size:
1701 1104 977 717
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 1804 3371 1804
Size: 1567 2932
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 229 343 472 1282 1503 3852 4108 229
Size: 114 129 810 221 2349 256 620
Fragments arranged by size:
2349 810 620 256 221 129 114
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 506 1519 506
Size: 1013 3486
BamHI G'GATC_C
Cuts at: 1524 1524
Size: 4499
BanI G'GyrC_C
Cuts at: 1515 1929 2177 2212 1515
Size: 414 248 35 3802
Fragments arranged by size:
3802 414 248 35
BanII G_rGCy'C
Cuts at: 265 1513 2543 265
Size: 1248 1030 2221
Fragments arranged by size:
2221 1248 1030
BbeI G_GCGC'C
Cuts at: 1933 2181 1933
Size: 248 4251
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3158 4499 2397
Size: 761 1341 2397
Fragments arranged by size:
2397 1341 761
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BglI GCCn_nnn'nGGC
Cuts at: 523 523
Size: 4499
BglII A'GATC_T
Cuts at: 2014 4051 2014
Size: 2037 2462
BlpI GC'TnA_GC
Cuts at: 302 1226 1611 302
Size: 924 385 3190
Fragments arranged by size:
3190 924 385
Bme1580I G_kGCm'C
Cuts at: 2124 2217 3273 2124
Size: 93 1056 3350
Fragments arranged by size:
3350 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 544 1711 1792 2127 4271 544
Size: 1167 81 335 2144 772
Fragments arranged by size:
2144 1167 772 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1028 1368 2866 1028
Size: 340 1498 2661
Fragments arranged by size:
2661 1498 340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 841 935 1466 733
Size: 108 94 531 3766
Fragments arranged by size:
3766 531 108 94
Bpu10I CC'TnA_GC
Cuts at: 1133 1133
Size: 4499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1675 2554 3046 3344 814
Size: 861 879 492 298 1969
Fragments arranged by size:
1969 879 861 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 343 4203 343
Size: 3860 639
BsaAI yAC'GTr
Cuts at: 153 1852 153
Size: 1699 2800
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 1930 2178 2880 4376 1930
Size: 248 702 1496 2053
Fragments arranged by size:
2053 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1688 2209 2887 3161 3308 3719 1688
Size: 521 678 274 147 411 2468
Fragments arranged by size:
2468 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 933 963 933
Size: 30 4469
BseYI C'CCAG_C
Cuts at: 1924 2682 3259 3755 1924
Size: 758 577 496 2668
Fragments arranged by size:
2668 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3295 104
Size: 1937 46 1208 1308
Fragments arranged by size:
1937 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1513 2291 2481 3273 1513
Size: 778 190 792 2739
Fragments arranged by size:
2739 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 265 1513 2124 2217 2291 2481 2543 3273 265
Size: 1248 611 93 74 190 62 730 1491
Fragments arranged by size:
1491 1248 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1549 1763 2065 2446 1549
Size: 214 302 381 3602
Fragments arranged by size:
3602 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2791 2845 4329 4494 50
Size: 2741 54 1484 165 55
Fragments arranged by size:
2741 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 777 1208 2411 777
Size: 431 1203 2865
Fragments arranged by size:
2865 1203 431
BsrFI r'CCGG_y
Cuts at: 1171 2497 2678 2887 1171
Size: 1326 181 209 2783
Fragments arranged by size:
2783 1326 209 181
BssSI C'ACGA_G
Cuts at: 2770 3128 2770
Size: 358 4141
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 1876
Size: 4499
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstEII G'GTnAC_C
Cuts at: 939 1458 939
Size: 519 3980
BstYI r'GATC_y
Cuts at: 1 1524 1991 2014 2349 2595 3607 3693 3705
Size: 1523 467 23 335 246 1012 86 12
Cuts at: 3705 4051 1
Size: 346 449
Fragments arranged by size:
1523 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 1127 1433 1742 1127
Size: 306 309 3884
Fragments arranged by size:
3884 309 306
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 759 774 794 809 858 893 759
Size: 15 20 15 49 35 4365
Fragments arranged by size:
4365 49 35 20 15 15
DraI TTT'AAA
Cuts at: 1498 1498
Size: 4499
DraIII CAC_nnn'GTG
Cuts at: 538 538
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 442 2205 3063 442
Size: 1763 858 1878
Fragments arranged by size:
1878 1763 858
EaeI y'GGCC_r
Cuts at: 180 900 2084 2258 2649 2676 4021 180
Size: 720 1184 174 391 27 1345 658
Fragments arranged by size:
1345 1184 720 658 391 174 27
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 595 1019 2522 2732 595
Size: 424 1503 210 2362
Fragments arranged by size:
2362 1503 424 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2707 3017 3163 4309 2707
Size: 310 146 1146 2897
Fragments arranged by size:
2897 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1511 1511
Size: 4499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 841 935 1252 1466 2324 2756 3503 3771
Size: 108 94 317 214 858 432 747 268
Cuts at: 3771 733
Size: 1461
Fragments arranged by size:
1461 858 747 432 317 268 214 108
94
EcoO109I rG'GnC_Cy
Cuts at: 1638 1638
Size: 4499
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
EcoRV GAT'ATC
Cuts at: 330 330
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 282 2518 282
Size: 2236 2263
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 1172 1933 2181 3203 4182 1172
Size: 761 248 1022 979 1489
Fragments arranged by size:
1489 1022 979 761 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 386 418 737 769 2341 2371 2373 2403 386
Size: 32 319 32 1572 30 2 30 2482
Fragments arranged by size:
2482 1572 319 32 32 30 30 2
HincII GTy'rAC
Cuts at: 564 957 1538 564
Size: 393 581 3525
Fragments arranged by size:
3525 581 393
HindIII A'AGCT_T
Cuts at: 998 998
Size: 4499
HpaI GTT'AAC
Cuts at: 564 564
Size: 4499
KasI G'GCGC_C
Cuts at: 1929 2177 1929
Size: 248 4251
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 673 853 3170 3354 673
Size: 180 2317 184 1818
Fragments arranged by size:
2317 1818 184 180
MscI TGG'CCA
Cuts at: 182 2260 182
Size: 2078 2421
MslI CAynn'nnrTG
Cuts at: 2488 2615 2488
Size: 127 4372
MspA1I CmG'CkG
Cuts at: 1120 1610 1924 2284 3297 3542 1120
Size: 490 314 360 1013 245 2077
Fragments arranged by size:
2077 1013 490 360 314 245
NaeI GCC'GGC
Cuts at: 1173 2680 1173
Size: 1507 2992
NarI GG'CG_CC
Cuts at: 1930 2178 1930
Size: 248 4251
NcoI C'CATG_G
Cuts at: 107 107
Size: 4499
NgoMIV G'CCGG_C
Cuts at: 1171 2678 1171
Size: 1507 2992
NspI r_CATG'y
Cuts at: 517 711 732 1217 1552 2052 2959 517
Size: 194 21 485 335 500 907 2057
Fragments arranged by size:
2057 907 500 485 335 194 21
PciI A'CATG_T
Cuts at: 2955 2955
Size: 4499
PflMI CCAn_nnn'nTGG
Cuts at: 688 688
Size: 4499
PfoI T'CCnGG_A
Cuts at: 857 857
Size: 4499
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PstI C_TGCA'G
Cuts at: 1546 2231 1546
Size: 685 3814
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 1120 1924 2284 1120
Size: 804 360 3335
Fragments arranged by size:
3335 804 360
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 1513 1513
Size: 4499
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 1019 2522 2732 1019
Size: 1503 210 2786
Fragments arranged by size:
2786 1503 210
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 361 4016 361
Size: 3655 844
SfcI C'TryA_G
Cuts at: 33 591 1542 2227 3220 3411 33
Size: 558 951 685 993 191 1121
Fragments arranged by size:
1121 993 951 685 558 191
SfoI GGC'GCC
Cuts at: 1931 2179 1931
Size: 248 4251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 793 1654 2569 3061 3323 793
Size: 861 915 492 262 1969
Fragments arranged by size:
1969 915 861 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 517 1217 1552 2052 517
Size: 700 335 500 2964
Fragments arranged by size:
2964 700 500 335
StyI C'CwwG_G
Cuts at: 107 733 1633 1987 107
Size: 626 900 354 2619
Fragments arranged by size:
2619 900 626 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 526 1402 2082 2215 2674 2883 526
Size: 876 680 133 459 209 2142
Fragments arranged by size:
2142 876 680 459 209 133
TatI w'GTAC_w
Cuts at: 359 4014 359
Size: 3655 844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 933 965 4016 4048 933
Size: 32 3051 32 1384
Fragments arranged by size:
3051 1384 32 32
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 689 1314 1994 689
Size: 625 680 3194
Fragments arranged by size:
3194 680 625
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
XmnI GAAnn'nnTTC
Cuts at: 1013 1013
Size: 4499
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI EcoNI FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI