Restriction map for
pF1KB6696
(Circular) (Six-base) MAPSORT of: pF1KB6696.seq Check: 1079 from: 1 to: 4499
pF1KB6696 4499 bp
With 182 enzymes: SgfI *
November 19, 2009 09:54 ..
AccI GT'mk_AC
Cuts at: 1537 1537
Size: 4499
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1015 1323 2324 2756 3503 3771 869
Size: 146 308 1001 432 747 268 1597
Fragments arranged by size:
1597 1001 747 432 308 268 146
AfeI AGC'GCT
Cuts at: 306 306
Size: 4499
AflIII A'CryG_T
Cuts at: 749 1851 2955 3932 749
Size: 1102 1104 977 1316
Fragments arranged by size:
1316 1104 1102 977
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AhdI GACnn_n'nnGTC
Cuts at: 482 482
Size: 4499
AleI CACnn'nnGTG
Cuts at: 269 269
Size: 4499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 287 287
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 192 1480 1804 3371 192
Size: 1288 324 1567 1320
Fragments arranged by size:
1567 1320 1288 324
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 1394 1503 3852 4108 1394
Size: 109 2349 256 1785
Fragments arranged by size:
2349 1785 256 109
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 1519 1519
Size: 4499
BamHI G'GATC_C
Cuts at: 875 1524 875
Size: 649 3850
BanI G'GyrC_C
Cuts at: 271 1515 1929 2177 2212 271
Size: 1244 414 248 35 2558
Fragments arranged by size:
2558 1244 414 248 35
BanII G_rGCy'C
Cuts at: 548 1513 2543 548
Size: 965 1030 2504
Fragments arranged by size:
2504 1030 965
BbeI G_GCGC'C
Cuts at: 1933 2181 1933
Size: 248 4251
BbsI GAAGACnn'nnnn_
Cuts at: 604 889 604
Size: 285 4214
BbvCI CC'TCA_GC
Cuts at: 218 218
Size: 4499
BciVI GTATCCnnnnn_n'
Cuts at: 1363 2397 3158 4499 1363
Size: 1034 761 1341 1363
Fragments arranged by size:
1363 1341 1034 761
BclI T'GATC_A
Cuts at: 494 2019 494
Size: 1525 2974
BfrBI ATG'CAT
Cuts at: 1309 1309
Size: 4499
BglI GCCn_nnn'nGGC
Cuts at: 280 280
Size: 4499
BglII A'GATC_T
Cuts at: 998 2014 4051 998
Size: 1016 2037 1446
Fragments arranged by size:
2037 1446 1016
BlpI GC'TnA_GC
Cuts at: 119 1611 119
Size: 1492 3007
Bme1580I G_kGCm'C
Cuts at: 276 472 2124 2217 3273 276
Size: 196 1652 93 1056 1502
Fragments arranged by size:
1652 1502 1056 196 93
BmrI ACTGGGnnnn_n'
Cuts at: 990 1291 1711 1792 2127 4271 990
Size: 301 420 81 335 2144 1218
Fragments arranged by size:
2144 1218 420 335 301 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
Bpu10I CC'TnA_GC
Cuts at: 218 645 218
Size: 427 4072
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 336 1060 1675 2554 3046 3344 336
Size: 724 615 879 492 298 1491
Fragments arranged by size:
1491 879 724 615 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1406 4203 1406
Size: 2797 1702
BsaAI yAC'GTr
Cuts at: 1852 1852
Size: 4499
BsaBI GATnn'nnATC
Cuts at: 6 727 2013 4050 6
Size: 721 1286 2037 455
Fragments arranged by size:
2037 1286 721 455
BsaHI Gr'CG_yC
Cuts at: 1930 2178 2880 4376 1930
Size: 248 702 1496 2053
Fragments arranged by size:
2053 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1688 2209 2887 3161 3308 3719 1688
Size: 521 678 274 147 411 2468
Fragments arranged by size:
2468 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1405 1435 1405
Size: 30 4469
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 136
Size: 4499
BseYI C'CCAG_C
Cuts at: 363 370 1924 2682 3259 3755 363
Size: 7 1554 758 577 496 1107
Fragments arranged by size:
1554 1107 758 577 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 759 759
Size: 4499
BsiEI CG_ry'CG
Cuts at: 104 284 479 2041 2087 3295 104
Size: 180 195 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 195 180 46
BsiHKAI G_wGCw'C
Cuts at: 251 1037 1513 2291 2481 3273 251
Size: 786 476 778 190 792 1477
Fragments arranged by size:
1477 792 786 778 476 190
Bsp1286I G_dGCh'C
Cuts at: 251 276 472 548 1037 1513 2124 2217 2291
Size: 25 196 76 489 476 611 93 74
Cuts at: 2291 2481 2543 3273 251
Size: 190 62 730 1477
Fragments arranged by size:
1477 730 611 489 476 196 190 93
76 74 62 25
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 998 1549 1763 2065 2446 998
Size: 551 214 302 381 3051
Fragments arranged by size:
3051 551 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2791 2845 4329 4494 50
Size: 2741 54 1484 165 55
Fragments arranged by size:
2741 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 797 1262 2411 797
Size: 465 1149 2885
Fragments arranged by size:
2885 1149 465
BsrFI r'CCGG_y
Cuts at: 478 2497 2678 2887 478
Size: 2019 181 209 2090
Fragments arranged by size:
2090 2019 209 181
BsrGI T'GTAC_A
Cuts at: 949 949
Size: 4499
BssSI C'ACGA_G
Cuts at: 2770 3128 2770
Size: 358 4141
BstAPI GCAn_nnn'nTGC
Cuts at: 1480 1876 1480
Size: 396 4103
BstBI TT'CG_AA
Cuts at: 1125 2860 1125
Size: 1735 2764
BstXI CCAn_nnnn'nTGG
Cuts at: 406 1437 406
Size: 1031 3468
BstYI r'GATC_y
Cuts at: 1 875 998 1524 1991 2014 2349 2595 3607
Size: 874 123 526 467 23 335 246 1012
Cuts at: 3607 3693 3705 4051 1
Size: 86 12 346 449
Fragments arranged by size:
1012 874 526 467 449 346 335 246
123 86 23 12
BtgI C'CryG_G
Cuts at: 293 1424 293
Size: 1131 3368
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 1742 1742
Size: 4499
ClaI AT'CG_AT
Cuts at: 792 792
Size: 4499
DraI TTT'AAA
Cuts at: 1498 1498
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3063 2205
Size: 858 3641
EaeI y'GGCC_r
Cuts at: 281 500 697 1427 2084 2258 2649 2676 4021
Size: 219 197 730 657 174 391 27 1345
Cuts at: 4021 281
Size: 759
Fragments arranged by size:
1345 759 730 657 391 219 197 174
27
EagI C'GGCC_G
Cuts at: 281 2084 281
Size: 1803 2696
EarI CTCTTCn'nnn_
Cuts at: 553 888 1368 2522 2732 553
Size: 335 480 1154 210 2320
Fragments arranged by size:
2320 1154 480 335 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 124 2707 3017 3163 4309 124
Size: 2583 310 146 1146 314
Fragments arranged by size:
2583 1146 314 310 146
EcoICRI GAG'CTC
Cuts at: 1511 1511
Size: 4499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1015 1323 2324 2756 3503 3771 869
Size: 146 308 1001 432 747 268 1597
Fragments arranged by size:
1597 1001 747 432 308 268 146
EcoO109I rG'GnC_Cy
Cuts at: 1638 1638
Size: 4499
EcoRI G'AATT_C
Cuts at: 1394 1503 1394
Size: 109 4390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4499
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 226 308 1933 2181 3203 4182 226
Size: 82 1625 248 1022 979 543
Fragments arranged by size:
1625 1022 979 543 248 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 468 500 774 806 1049 1081 1405 1437 2341
Size: 32 274 32 243 32 324 32 904
Cuts at: 2341 2371 2373 2403 468
Size: 30 2 30 2564
Fragments arranged by size:
2564 904 324 274 243 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 390 1538 390
Size: 1148 3351
KasI G'GCGC_C
Cuts at: 1929 2177 1929
Size: 248 4251
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MfeI C'AATT_G
Cuts at: 327 327
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 443 499 994 1391 3170 3354 443
Size: 56 495 397 1779 184 1588
Fragments arranged by size:
1779 1588 495 397 184 56
MscI TGG'CCA
Cuts at: 699 2260 699
Size: 1561 2938
MslI CAynn'nnrTG
Cuts at: 269 404 511 571 592 1045 1138 2488 2615
Size: 135 107 60 21 453 93 1350 127
Cuts at: 2615 269
Size: 2153
Fragments arranged by size:
2153 1350 453 135 127 107 93 60
21
MspA1I CmG'CkG
Cuts at: 201 269 1286 1610 1924 2284 3297 3542 201
Size: 68 1017 324 314 360 1013 245 1158
Fragments arranged by size:
1158 1017 1013 360 324 314 245 68
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1930 2178 1930
Size: 248 4251
NcoI C'CATG_G
Cuts at: 293 293
Size: 4499
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NsiI A_TGCA'T
Cuts at: 1311 1311
Size: 4499
NspI r_CATG'y
Cuts at: 753 1092 1552 2052 2959 753
Size: 339 460 500 907 2293
Fragments arranged by size:
2293 907 500 460 339
PciI A'CATG_T
Cuts at: 749 2955 749
Size: 2206 2293
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PstI C_TGCA'G
Cuts at: 1546 2231 1546
Size: 685 3814
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 1286 1924 2284 1286
Size: 638 360 3501
Fragments arranged by size:
3501 638 360
RsrII CG'GwC_CG
Cuts at: 430 2694 430
Size: 2264 2235
SacI G_AGCT'C
Cuts at: 1513 1513
Size: 4499
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 553 2522 2732 553
Size: 1969 210 2320
Fragments arranged by size:
2320 1969 210
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SexAI A'CCwGG_T
Cuts at: 973 973
Size: 4499
SfcI C'TryA_G
Cuts at: 33 1542 2227 3220 3411 33
Size: 1509 685 993 191 1121
Fragments arranged by size:
1509 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1931 2179 1931
Size: 248 4251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 351 1075 1654 2569 3061 3323 351
Size: 724 579 915 492 262 1527
Fragments arranged by size:
1527 915 724 579 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1092 1552 2052 1092
Size: 460 500 3539
Fragments arranged by size:
3539 500 460
StuI AGG'CCT
Cuts at: 823 823
Size: 4499
StyI C'CwwG_G
Cuts at: 293 341 1211 1633 1987 293
Size: 48 870 422 354 2805
Fragments arranged by size:
2805 870 422 354 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 115 384 2082 2215 2674 2883 115
Size: 269 1698 133 459 209 1731
Fragments arranged by size:
1731 1698 459 269 209 133
TatI w'GTAC_w
Cuts at: 949 4014 949
Size: 3065 1434
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4467
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 510 534 977 1143 1994 510
Size: 24 443 166 851 3015
Fragments arranged by size:
3015 851 443 166 24
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AloI ApaI AscI AvrII BaeI
BcgI BmgBI BmtI BpmI BsiWI BsmI BsmBI BssHII
BstEII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI