Restriction map for
pF1KB6699
(Circular) (Six-base) MAPSORT of: pF1KB6699.seq Check: 4845 from: 1 to: 4025
pF1KB6699 4025 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 845 845
Size: 4025
AccI GT'mk_AC
Cuts at: 1063 1063
Size: 4025
Acc65I G'GTAC_C
Cuts at: 1041 1041
Size: 4025
AclI AA'CG_TT
Cuts at: 3865 3865
Size: 4025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1850 2282 3029 3297 1850
Size: 432 747 268 2578
Fragments arranged by size:
2578 747 432 268
AflIII A'CryG_T
Cuts at: 575 1377 2481 3458 575
Size: 802 1104 977 1142
Fragments arranged by size:
1142 1104 977 802
AgeI A'CCGG_T
Cuts at: 820 2413 820
Size: 1593 2432
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 404 404
Size: 4025
AlwNI CAG_nnn'CTG
Cuts at: 1330 2897 1330
Size: 1567 2458
ApaI G_GGCC'C
Cuts at: 517 632 673 517
Size: 115 41 3869
Fragments arranged by size:
3869 115 41
ApaLI G'TGCA_C
Cuts at: 2795 2795
Size: 4025
ApoI r'AATT_y
Cuts at: 1029 3378 3634 1029
Size: 2349 256 1420
Fragments arranged by size:
2349 1420 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4025
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 741 1045 741
Size: 304 3721
BamHI G'GATC_C
Cuts at: 1050 1050
Size: 4025
BanI G'GyrC_C
Cuts at: 1041 1455 1703 1738 1041
Size: 414 248 35 3328
Fragments arranged by size:
3328 414 248 35
BanII G_rGCy'C
Cuts at: 517 568 632 673 1039 2069 517
Size: 51 64 41 366 1030 2473
Fragments arranged by size:
2473 1030 366 64 51 41
BbeI G_GCGC'C
Cuts at: 1459 1707 1459
Size: 248 3777
BciVI GTATCCnnnnn_n'
Cuts at: 1923 2684 4025 1923
Size: 761 1341 1923
Fragments arranged by size:
1923 1341 761
BclI T'GATC_A
Cuts at: 1545 1545
Size: 4025
BglII A'GATC_T
Cuts at: 1540 3577 1540
Size: 2037 1988
BlpI GC'TnA_GC
Cuts at: 1137 1137
Size: 4025
Bme1580I G_kGCm'C
Cuts at: 517 632 673 1650 1743 2799 517
Size: 115 41 977 93 1056 1743
Fragments arranged by size:
1743 1056 977 115 93 41
BmrI ACTGGGnnnn_n'
Cuts at: 443 1237 1318 1653 3797 443
Size: 794 81 335 2144 671
Fragments arranged by size:
2144 794 671 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 4025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1201 2080 2572 2870 1201
Size: 879 492 298 2356
Fragments arranged by size:
2356 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 351 3729 351
Size: 3378 647
BsaAI yAC'GTr
Cuts at: 1378 1378
Size: 4025
BsaBI GATnn'nnATC
Cuts at: 6 1539 3576 6
Size: 1533 2037 455
Fragments arranged by size:
2037 1533 455
BsaHI Gr'CG_yC
Cuts at: 1456 1704 2406 3902 1456
Size: 248 702 1496 1579
Fragments arranged by size:
1579 1496 702 248
BsaWI w'CCGG_w
Cuts at: 820 1214 1735 2413 2687 2834 3245 820
Size: 394 521 678 274 147 411 1600
Fragments arranged by size:
1600 678 521 411 394 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 182 212 524 554 182
Size: 30 312 30 3653
Fragments arranged by size:
3653 312 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 877 877
Size: 4025
BseYI C'CCAG_C
Cuts at: 506 1450 2208 2785 3281 506
Size: 944 758 577 496 1250
Fragments arranged by size:
1250 944 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 4025
BsiEI CG_ry'CG
Cuts at: 104 1567 1613 2821 104
Size: 1463 46 1208 1308
Fragments arranged by size:
1463 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 278 335 568 916 1039 1817 2007 2799 278
Size: 57 233 348 123 778 190 792 1504
Fragments arranged by size:
1504 792 778 348 233 190 123 57
Bsp1286I G_dGCh'C
Cuts at: 278 335 517 568 632 673 916 1039 1650
Size: 57 182 51 64 41 243 123 611
Cuts at: 1650 1743 1817 2007 2069 2799 278
Size: 93 74 190 62 730 1504
Fragments arranged by size:
1504 730 611 243 190 182 123 93
74 64 62 57 51 41
BspEI T'CCGG_A
Cuts at: 3245 3245
Size: 4025
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 845 1075 1289 1591 1972 845
Size: 230 214 302 381 2898
Fragments arranged by size:
2898 381 302 230 214
BsrBI CCG'CTC
Cuts at: 50 2317 2371 3855 4020 50
Size: 2267 54 1484 165 55
Fragments arranged by size:
2267 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 256 1937 256
Size: 1681 2344
BsrFI r'CCGG_y
Cuts at: 820 2023 2204 2413 820
Size: 1203 181 209 2432
Fragments arranged by size:
2432 1203 209 181
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 1402 1402
Size: 4025
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstEII G'GTnAC_C
Cuts at: 823 823
Size: 4025
BstXI CCAn_nnnn'nTGG
Cuts at: 525 525
Size: 4025
BstYI r'GATC_y
Cuts at: 1 1050 1517 1540 1875 2121 3133 3219 3231
Size: 1049 467 23 335 246 1012 86 12
Cuts at: 3231 3577 1
Size: 346 449
Fragments arranged by size:
1049 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 518 681 518
Size: 163 3862
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 889 1975 2156 2319 889
Size: 1086 181 163 2595
Fragments arranged by size:
2595 1086 181 163
BtsI GCAGTG_nn'
Cuts at: 751 1268 751
Size: 517 3508
DraI TTT'AAA
Cuts at: 1024 1024
Size: 4025
DrdI GACnn_nn'nnGTC
Cuts at: 1731 2589 1731
Size: 858 3167
EaeI y'GGCC_r
Cuts at: 166 1610 1784 2175 2202 3547 166
Size: 1444 174 391 27 1345 644
Fragments arranged by size:
1444 1345 644 391 174 27
EagI C'GGCC_G
Cuts at: 1610 1610
Size: 4025
EarI CTCTTCn'nnn_
Cuts at: 227 895 2048 2258 227
Size: 668 1153 210 1994
Fragments arranged by size:
1994 1153 668 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2233 2543 2689 3835 2233
Size: 310 146 1146 2423
Fragments arranged by size:
2423 1146 310 146
EcoICRI GAG'CTC
Cuts at: 566 1037 566
Size: 471 3554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1850 2282 3029 3297 1850
Size: 432 747 268 2578
Fragments arranged by size:
2578 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 161 214 395 514 628 670 854 1164 161
Size: 53 181 119 114 42 184 310 3022
Fragments arranged by size:
3022 310 184 181 119 114 53 42
EcoRI G'AATT_C
Cuts at: 1029 1029
Size: 4025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 375 2044 375
Size: 1669 2356
FspI TGC'GCA
Cuts at: 1806 1806
Size: 4025
HaeII r_GCGC'y
Cuts at: 1459 1707 2729 3708 1459
Size: 248 1022 979 1776
Fragments arranged by size:
1776 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 363 1867 1897 1899 1929 331
Size: 32 1504 30 2 30 2427
Fragments arranged by size:
2427 1504 32 30 30 2
HincII GTy'rAC
Cuts at: 639 1064 639
Size: 425 3600
KasI G'GCGC_C
Cuts at: 1455 1703 1455
Size: 248 3777
KpnI G_GTAC'C
Cuts at: 1045 1045
Size: 4025
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 941 2696 2880 941
Size: 1755 184 2086
Fragments arranged by size:
2086 1755 184
MscI TGG'CCA
Cuts at: 168 1786 168
Size: 1618 2407
MslI CAynn'nnrTG
Cuts at: 338 839 2014 2141 338
Size: 501 1175 127 2222
Fragments arranged by size:
2222 1175 501 127
MspA1I CmG'CkG
Cuts at: 1136 1450 1810 2823 3068 1136
Size: 314 360 1013 245 2093
Fragments arranged by size:
2093 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 4025
NarI GG'CG_CC
Cuts at: 1456 1704 1456
Size: 248 3777
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 4025
NspI r_CATG'y
Cuts at: 579 844 1078 1578 2485 579
Size: 265 234 500 907 2119
Fragments arranged by size:
2119 907 500 265 234
PciI A'CATG_T
Cuts at: 575 2481 575
Size: 1906 2119
PfoI T'CCnGG_A
Cuts at: 559 559
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3993
PpuMI rG'GwC_Cy
Cuts at: 214 395 854 214
Size: 181 459 3385
Fragments arranged by size:
3385 459 181
PspOMI G'GGCC_C
Cuts at: 513 628 669 513
Size: 115 41 3869
Fragments arranged by size:
3869 115 41
PspXI vC'TCGA_Gb
Cuts at: 741 741
Size: 4025
PstI C_TGCA'G
Cuts at: 158 556 627 957 1072 1757 158
Size: 398 71 330 115 685 2426
Fragments arranged by size:
2426 685 398 330 115 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 1450 1810 1450
Size: 360 3665
RsrII CG'GwC_CG
Cuts at: 2220 2220
Size: 4025
SacI G_AGCT'C
Cuts at: 568 1039 568
Size: 471 3554
SalI G'TCGA_C
Cuts at: 1062 1062
Size: 4025
SapI GCTCTTCn'nnn_
Cuts at: 2048 2258 2048
Size: 210 3815
SbfI CC_TGCA'GG
Cuts at: 957 1072 957
Size: 115 3910
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SfcI C'TryA_G
Cuts at: 33 154 399 552 623 693 953 1068 1753
Size: 121 245 153 71 70 260 115 685
Cuts at: 1753 2746 2937 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 260 245 191 153 121
115 71 70
SfoI GGC'GCC
Cuts at: 1457 1705 1457
Size: 248 3777
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SmaI CCC'GGG
Cuts at: 1047 1047
Size: 4025
SmlI C'TyrA_G
Cuts at: 741 1180 2095 2587 2849 741
Size: 439 915 492 262 1917
Fragments arranged by size:
1917 915 492 439 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 1078 1578 1078
Size: 500 3525
StyI C'CwwG_G
Cuts at: 350 632 1159 1513 350
Size: 282 527 354 2862
Fragments arranged by size:
2862 527 354 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 224 358 694 813 1608 1741 2200 2409 224
Size: 134 336 119 795 133 459 209 1840
Fragments arranged by size:
1840 795 459 336 209 134 133 119
TatI w'GTAC_w
Cuts at: 3540 3540
Size: 4025
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 524 556 3542 3574 524
Size: 32 2986 32 975
Fragments arranged by size:
2986 975 32 32
Tth111I GACn'n_nGTC
Cuts at: 466 546 1822 466
Size: 80 1276 2669
Fragments arranged by size:
2669 1276 80
XbaI T'CTAG_A
Cuts at: 1056 1056
Size: 4025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 456 1520 114
Size: 342 1064 2619
Fragments arranged by size:
2619 1064 342
XhoI C'TCGA_G
Cuts at: 741 741
Size: 4025
XmaI C'CCGG_G
Cuts at: 1045 1045
Size: 4025
XmnI GAAnn'nnTTC
Cuts at: 192 777 192
Size: 585 3440
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI ZraI