Restriction map for
pF1KB6701
(Circular) (Six-base) MAPSORT of: pF1KB6701.seq Check: 5337 from: 1 to: 3299
pF1KB6701 3299 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 127 160 127
Size: 33 3266
AccI GT'mk_AC
Cuts at: 337 337
Size: 3299
Acc65I G'GTAC_C
Cuts at: 315 315
Size: 3299
AclI AA'CG_TT
Cuts at: 3139 3139
Size: 3299
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1124 1556 2303 2571 1124
Size: 432 747 268 1852
Fragments arranged by size:
1852 747 432 268
AflIII A'CryG_T
Cuts at: 651 1755 2732 651
Size: 1104 977 1218
Fragments arranged by size:
1218 1104 977
AgeI A'CCGG_T
Cuts at: 1687 1687
Size: 3299
AlwNI CAG_nnn'CTG
Cuts at: 164 287 604 2171 164
Size: 123 317 1567 1292
Fragments arranged by size:
1567 1292 317 123
ApaLI G'TGCA_C
Cuts at: 2069 2069
Size: 3299
ApoI r'AATT_y
Cuts at: 303 2652 2908 303
Size: 2349 256 694
Fragments arranged by size:
2349 694 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3299
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3299
AvaI C'yCGr_G
Cuts at: 319 319
Size: 3299
BamHI G'GATC_C
Cuts at: 324 324
Size: 3299
BanI G'GyrC_C
Cuts at: 315 729 977 1012 315
Size: 414 248 35 2602
Fragments arranged by size:
2602 414 248 35
BanII G_rGCy'C
Cuts at: 313 1343 313
Size: 1030 2269
BbeI G_GCGC'C
Cuts at: 733 981 733
Size: 248 3051
BciVI GTATCCnnnnn_n'
Cuts at: 1197 1958 3299 1197
Size: 761 1341 1197
Fragments arranged by size:
1341 1197 761
BclI T'GATC_A
Cuts at: 819 819
Size: 3299
BglI GCCn_nnn'nGGC
Cuts at: 227 227
Size: 3299
BglII A'GATC_T
Cuts at: 814 2851 814
Size: 2037 1262
BlpI GC'TnA_GC
Cuts at: 411 411
Size: 3299
Bme1580I G_kGCm'C
Cuts at: 246 924 1017 2073 246
Size: 678 93 1056 1472
Fragments arranged by size:
1472 1056 678 93
BmrI ACTGGGnnnn_n'
Cuts at: 511 592 927 3071 511
Size: 81 335 2144 739
Fragments arranged by size:
2144 739 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1666 1666
Size: 3299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 1354 1846 2144 475
Size: 879 492 298 1630
Fragments arranged by size:
1630 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3003 3003
Size: 3299
BsaAI yAC'GTr
Cuts at: 652 652
Size: 3299
BsaBI GATnn'nnATC
Cuts at: 6 813 2850 6
Size: 807 2037 455
Fragments arranged by size:
2037 807 455
BsaHI Gr'CG_yC
Cuts at: 730 978 1680 3176 730
Size: 248 702 1496 853
Fragments arranged by size:
1496 853 702 248
BsaWI w'CCGG_w
Cuts at: 488 1009 1687 1961 2108 2519 488
Size: 521 678 274 147 411 1268
Fragments arranged by size:
1268 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 724 1482 2059 2555 724
Size: 758 577 496 1468
Fragments arranged by size:
1468 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 300 132
Size: 168 3131
BsiEI CG_ry'CG
Cuts at: 104 841 887 2095 104
Size: 737 46 1208 1308
Fragments arranged by size:
1308 1208 737 46
BsiHKAI G_wGCw'C
Cuts at: 313 1091 1281 2073 313
Size: 778 190 792 1539
Fragments arranged by size:
1539 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 246 313 924 1017 1091 1281 1343 2073 246
Size: 67 611 93 74 190 62 730 1472
Fragments arranged by size:
1472 730 611 190 93 74 67 62
BspEI T'CCGG_A
Cuts at: 2519 2519
Size: 3299
BspHI T'CATG_A
Cuts at: 2475 2475
Size: 3299
BspMI ACCTGCnnnn'nnnn_
Cuts at: 127 160 349 563 865 1246 127
Size: 33 189 214 302 381 2180
Fragments arranged by size:
2180 381 302 214 189 33
BsrBI CCG'CTC
Cuts at: 50 1591 1645 3129 3294 50
Size: 1541 54 1484 165 55
Fragments arranged by size:
1541 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1211 1211
Size: 3299
BsrFI r'CCGG_y
Cuts at: 1297 1478 1687 1297
Size: 181 209 2909
Fragments arranged by size:
2909 209 181
BssSI C'ACGA_G
Cuts at: 1570 1928 1570
Size: 358 2941
BstAPI GCAn_nnn'nTGC
Cuts at: 179 194 287 676 179
Size: 15 93 389 2802
Fragments arranged by size:
2802 389 93 15
BstBI TT'CG_AA
Cuts at: 1660 1660
Size: 3299
BstYI r'GATC_y
Cuts at: 1 324 791 814 1149 1395 2407 2493 2505
Size: 323 467 23 335 246 1012 86 12
Cuts at: 2505 2851 1
Size: 346 449
Fragments arranged by size:
1012 467 449 346 335 323 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1249 1430 1593 1249
Size: 181 163 2955
Fragments arranged by size:
2955 181 163
BtsI GCAGTG_nn'
Cuts at: 542 542
Size: 3299
DraI TTT'AAA
Cuts at: 298 298
Size: 3299
DrdI GACnn_nn'nnGTC
Cuts at: 1005 1863 1005
Size: 858 2441
EaeI y'GGCC_r
Cuts at: 884 1058 1449 1476 2821 884
Size: 174 391 27 1345 1362
Fragments arranged by size:
1362 1345 391 174 27
EagI C'GGCC_G
Cuts at: 884 884
Size: 3299
EarI CTCTTCn'nnn_
Cuts at: 196 1322 1532 196
Size: 1126 210 1963
Fragments arranged by size:
1963 1126 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1507 1817 1963 3109 1507
Size: 310 146 1146 1697
Fragments arranged by size:
1697 1146 310 146
EcoICRI GAG'CTC
Cuts at: 311 311
Size: 3299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1124 1556 2303 2571 1124
Size: 432 747 268 1852
Fragments arranged by size:
1852 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 438 438
Size: 3299
EcoRI G'AATT_C
Cuts at: 303 303
Size: 3299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1318 1318
Size: 3299
FspI TGC'GCA
Cuts at: 1080 1080
Size: 3299
HaeII r_GCGC'y
Cuts at: 733 981 2003 2982 733
Size: 248 1022 979 1050
Fragments arranged by size:
1050 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1141 1171 1173 1203 1141
Size: 30 2 30 3237
Fragments arranged by size:
3237 30 30 2
HincII GTy'rAC
Cuts at: 338 338
Size: 3299
KasI G'GCGC_C
Cuts at: 729 977 729
Size: 248 3051
KpnI G_GTAC'C
Cuts at: 319 319
Size: 3299
MluI A'CGCG_T
Cuts at: 2732 2732
Size: 3299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1970 2154 1970
Size: 184 3115
MscI TGG'CCA
Cuts at: 1060 1060
Size: 3299
MslI CAynn'nnrTG
Cuts at: 1288 1415 1288
Size: 127 3172
MspA1I CmG'CkG
Cuts at: 133 284 410 724 1084 2097 2342 133
Size: 151 126 314 360 1013 245 1090
Fragments arranged by size:
1090 1013 360 314 245 151 126
NaeI GCC'GGC
Cuts at: 1480 1480
Size: 3299
NarI GG'CG_CC
Cuts at: 730 978 730
Size: 248 3051
NcoI C'CATG_G
Cuts at: 107 107
Size: 3299
NgoMIV G'CCGG_C
Cuts at: 1478 1478
Size: 3299
NspI r_CATG'y
Cuts at: 352 852 1759 352
Size: 500 907 1892
Fragments arranged by size:
1892 907 500
PasI CC'CwG_GG
Cuts at: 246 246
Size: 3299
PciI A'CATG_T
Cuts at: 1755 1755
Size: 3299
PmeI GTTT'AAAC
Cuts at: 298 298
Size: 3299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2470 2438
Size: 32 3267
PstI C_TGCA'G
Cuts at: 138 346 1031 138
Size: 208 685 2406
Fragments arranged by size:
2406 685 208
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3299
PvuII CAG'CTG
Cuts at: 284 724 1084 284
Size: 440 360 2499
Fragments arranged by size:
2499 440 360
RsrII CG'GwC_CG
Cuts at: 1494 1494
Size: 3299
SacI G_AGCT'C
Cuts at: 313 313
Size: 3299
SalI G'TCGA_C
Cuts at: 336 336
Size: 3299
SapI GCTCTTCn'nnn_
Cuts at: 196 1322 1532 196
Size: 1126 210 1963
Fragments arranged by size:
1963 1126 210
SbfI CC_TGCA'GG
Cuts at: 346 346
Size: 3299
ScaI AGT'ACT
Cuts at: 2816 2816
Size: 3299
SfcI C'TryA_G
Cuts at: 33 134 342 1027 2020 2211 33
Size: 101 208 685 993 191 1121
Fragments arranged by size:
1121 993 685 208 191 101
SfoI GGC'GCC
Cuts at: 731 979 731
Size: 248 3051
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3299
SmaI CCC'GGG
Cuts at: 321 321
Size: 3299
SmlI C'TyrA_G
Cuts at: 454 1369 1861 2123 454
Size: 915 492 262 1630
Fragments arranged by size:
1630 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3299
SphI G_CATG'C
Cuts at: 352 852 352
Size: 500 2799
StyI C'CwwG_G
Cuts at: 107 433 787 107
Size: 326 354 2619
Fragments arranged by size:
2619 354 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 882 1015 1474 1683 882
Size: 133 459 209 2498
Fragments arranged by size:
2498 459 209 133
TatI w'GTAC_w
Cuts at: 2814 2814
Size: 3299
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2816 2848 2816
Size: 32 3267
Tth111I GACn'n_nGTC
Cuts at: 1096 1096
Size: 3299
XbaI T'CTAG_A
Cuts at: 330 330
Size: 3299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 794 794
Size: 3299
XmaI C'CCGG_G
Cuts at: 319 319
Size: 3299
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI