Restriction map for
pF1KB6704
(Circular) (Six-base) MAPSORT of: pF1KB6704.seq Check: 5079 from: 1 to: 3497
pF1KB6704 3497 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 535 535
Size: 3497
Acc65I G'GTAC_C
Cuts at: 513 513
Size: 3497
AclI AA'CG_TT
Cuts at: 3337 3337
Size: 3497
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 1754 2501 2769 1322
Size: 432 747 268 2050
Fragments arranged by size:
2050 747 432 268
AflIII A'CryG_T
Cuts at: 849 1953 2930 849
Size: 1104 977 1416
Fragments arranged by size:
1416 1104 977
AgeI A'CCGG_T
Cuts at: 1885 1885
Size: 3497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 211 211
Size: 3497
AlwNI CAG_nnn'CTG
Cuts at: 121 316 802 2369 121
Size: 195 486 1567 1249
Fragments arranged by size:
1567 1249 486 195
ApaLI G'TGCA_C
Cuts at: 2267 2267
Size: 3497
ApoI r'AATT_y
Cuts at: 501 2850 3106 501
Size: 2349 256 892
Fragments arranged by size:
2349 892 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3497
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3497
AvaI C'yCGr_G
Cuts at: 517 517
Size: 3497
BamHI G'GATC_C
Cuts at: 522 522
Size: 3497
BanI G'GyrC_C
Cuts at: 513 927 1175 1210 513
Size: 414 248 35 2800
Fragments arranged by size:
2800 414 248 35
BanII G_rGCy'C
Cuts at: 511 1541 511
Size: 1030 2467
BbeI G_GCGC'C
Cuts at: 931 1179 931
Size: 248 3249
BbvCI CC'TCA_GC
Cuts at: 199 426 199
Size: 227 3270
BciVI GTATCCnnnnn_n'
Cuts at: 416 1395 2156 3497 416
Size: 979 761 1341 416
Fragments arranged by size:
1341 979 761 416
BclI T'GATC_A
Cuts at: 1017 1017
Size: 3497
BglII A'GATC_T
Cuts at: 1012 3049 1012
Size: 2037 1460
BlpI GC'TnA_GC
Cuts at: 609 609
Size: 3497
Bme1580I G_kGCm'C
Cuts at: 1122 1215 2271 1122
Size: 93 1056 2348
Fragments arranged by size:
2348 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 185 484 709 790 1125 3269 185
Size: 299 225 81 335 2144 413
Fragments arranged by size:
2144 413 335 299 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1864 1864
Size: 3497
Bpu10I CC'TnA_GC
Cuts at: 199 426 199
Size: 227 3270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 1552 2044 2342 673
Size: 879 492 298 1828
Fragments arranged by size:
1828 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3201 3201
Size: 3497
BsaAI yAC'GTr
Cuts at: 850 850
Size: 3497
BsaBI GATnn'nnATC
Cuts at: 6 1011 3048 6
Size: 1005 2037 455
Fragments arranged by size:
2037 1005 455
BsaHI Gr'CG_yC
Cuts at: 928 1176 1878 3374 928
Size: 248 702 1496 1051
Fragments arranged by size:
1496 1051 702 248
BsaWI w'CCGG_w
Cuts at: 338 686 1207 1885 2159 2306 2717 338
Size: 348 521 678 274 147 411 1118
Fragments arranged by size:
1118 678 521 411 348 274 147
BseYI C'CCAG_C
Cuts at: 922 1680 2257 2753 922
Size: 758 577 496 1666
Fragments arranged by size:
1666 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1039 1085 2293 104
Size: 935 46 1208 1308
Fragments arranged by size:
1308 1208 935 46
BsiHKAI G_wGCw'C
Cuts at: 511 1289 1479 2271 511
Size: 778 190 792 1737
Fragments arranged by size:
1737 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 511 1122 1215 1289 1479 1541 2271 511
Size: 611 93 74 190 62 730 1737
Fragments arranged by size:
1737 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 338 2717 338
Size: 2379 1118
BspHI T'CATG_A
Cuts at: 2673 2673
Size: 3497
BspMI ACCTGCnnnn'nnnn_
Cuts at: 547 761 1063 1444 547
Size: 214 302 381 2600
Fragments arranged by size:
2600 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1789 1843 3327 3492 50
Size: 1739 54 1484 165 55
Fragments arranged by size:
1739 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1409 1409
Size: 3497
BsrFI r'CCGG_y
Cuts at: 151 1495 1676 1885 151
Size: 1344 181 209 1763
Fragments arranged by size:
1763 1344 209 181
BssSI C'ACGA_G
Cuts at: 1768 2126 1768
Size: 358 3139
BstAPI GCAn_nnn'nTGC
Cuts at: 874 874
Size: 3497
BstBI TT'CG_AA
Cuts at: 1858 1858
Size: 3497
BstXI CCAn_nnnn'nTGG
Cuts at: 180 180
Size: 3497
BstYI r'GATC_y
Cuts at: 1 465 522 989 1012 1347 1593 2605 2691
Size: 464 57 467 23 335 246 1012 86
Cuts at: 2691 2703 3049 1
Size: 12 346 449
Fragments arranged by size:
1012 467 464 449 346 335 246 86
57 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3497
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1447 1628 1791 1447
Size: 181 163 3153
Fragments arranged by size:
3153 181 163
BtsI GCAGTG_nn'
Cuts at: 122 740 122
Size: 618 2879
DraI TTT'AAA
Cuts at: 496 496
Size: 3497
DrdI GACnn_nn'nnGTC
Cuts at: 1203 2061 1203
Size: 858 2639
EaeI y'GGCC_r
Cuts at: 1082 1256 1647 1674 3019 1082
Size: 174 391 27 1345 1560
Fragments arranged by size:
1560 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1082 1082
Size: 3497
EarI CTCTTCn'nnn_
Cuts at: 1520 1730 1520
Size: 210 3287
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1705 2015 2161 3307 1705
Size: 310 146 1146 1895
Fragments arranged by size:
1895 1146 310 146
EcoICRI GAG'CTC
Cuts at: 509 509
Size: 3497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 1754 2501 2769 1322
Size: 432 747 268 2050
Fragments arranged by size:
2050 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 636 636
Size: 3497
EcoRI G'AATT_C
Cuts at: 501 501
Size: 3497
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1516 1516
Size: 3497
FspI TGC'GCA
Cuts at: 1278 1278
Size: 3497
HaeII r_GCGC'y
Cuts at: 931 1179 2201 3180 931
Size: 248 1022 979 1248
Fragments arranged by size:
1248 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 1339 1369 1371 1401 406
Size: 32 901 30 2 30 2502
Fragments arranged by size:
2502 901 32 30 30 2
HincII GTy'rAC
Cuts at: 536 536
Size: 3497
KasI G'GCGC_C
Cuts at: 927 1175 927
Size: 248 3249
KpnI G_GTAC'C
Cuts at: 517 517
Size: 3497
MluI A'CGCG_T
Cuts at: 2930 2930
Size: 3497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2168 2352 2168
Size: 184 3313
MscI TGG'CCA
Cuts at: 1258 1258
Size: 3497
MslI CAynn'nnrTG
Cuts at: 1486 1613 1486
Size: 127 3370
MspA1I CmG'CkG
Cuts at: 608 922 1282 2295 2540 608
Size: 314 360 1013 245 1565
Fragments arranged by size:
1565 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1678 1678
Size: 3497
NarI GG'CG_CC
Cuts at: 928 1176 928
Size: 248 3249
NcoI C'CATG_G
Cuts at: 107 107
Size: 3497
NgoMIV G'CCGG_C
Cuts at: 1676 1676
Size: 3497
NspI r_CATG'y
Cuts at: 550 1050 1957 550
Size: 500 907 2090
Fragments arranged by size:
2090 907 500
PciI A'CATG_T
Cuts at: 1953 1953
Size: 3497
PmeI GTTT'AAAC
Cuts at: 496 496
Size: 3497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2636 2668 2636
Size: 32 3465
PstI C_TGCA'G
Cuts at: 404 544 1229 404
Size: 140 685 2672
Fragments arranged by size:
2672 685 140
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3497
PvuII CAG'CTG
Cuts at: 922 1282 922
Size: 360 3137
RsrII CG'GwC_CG
Cuts at: 1692 1692
Size: 3497
SacI G_AGCT'C
Cuts at: 511 511
Size: 3497
SalI G'TCGA_C
Cuts at: 534 534
Size: 3497
SapI GCTCTTCn'nnn_
Cuts at: 1520 1730 1520
Size: 210 3287
SbfI CC_TGCA'GG
Cuts at: 544 544
Size: 3497
ScaI AGT'ACT
Cuts at: 3014 3014
Size: 3497
SfcI C'TryA_G
Cuts at: 33 400 449 540 1225 2218 2409 33
Size: 367 49 91 685 993 191 1121
Fragments arranged by size:
1121 993 685 367 191 91 49
SfoI GGC'GCC
Cuts at: 929 1177 929
Size: 248 3249
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3497
SmaI CCC'GGG
Cuts at: 519 519
Size: 3497
SmlI C'TyrA_G
Cuts at: 652 1567 2059 2321 652
Size: 915 492 262 1828
Fragments arranged by size:
1828 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3497
SphI G_CATG'C
Cuts at: 550 1050 550
Size: 500 2997
StyI C'CwwG_G
Cuts at: 107 631 985 107
Size: 524 354 2619
Fragments arranged by size:
2619 524 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1080 1213 1672 1881 1080
Size: 133 459 209 2696
Fragments arranged by size:
2696 459 209 133
TatI w'GTAC_w
Cuts at: 227 436 3012 227
Size: 209 2576 712
Fragments arranged by size:
2576 712 209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3465
Tth111I GACn'n_nGTC
Cuts at: 1294 1294
Size: 3497
XbaI T'CTAG_A
Cuts at: 528 528
Size: 3497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 992 114
Size: 878 2619
XmaI C'CCGG_G
Cuts at: 517 517
Size: 3497
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI