Restriction map for
pF1KB6706
(Circular) (Six-base) MAPSORT of: pF1KB6706.seq Check: 9069 from: 1 to: 3500
pF1KB6706 3500 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 538 538
Size: 3500
Acc65I G'GTAC_C
Cuts at: 516 516
Size: 3500
AclI AA'CG_TT
Cuts at: 3340 3340
Size: 3500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 1757 2504 2772 1325
Size: 432 747 268 2053
Fragments arranged by size:
2053 747 432 268
AflIII A'CryG_T
Cuts at: 492 852 1956 2933 492
Size: 360 1104 977 1059
Fragments arranged by size:
1104 1059 977 360
AgeI A'CCGG_T
Cuts at: 1888 1888
Size: 3500
AlwNI CAG_nnn'CTG
Cuts at: 805 2372 805
Size: 1567 1933
ApaLI G'TGCA_C
Cuts at: 2270 2270
Size: 3500
ApoI r'AATT_y
Cuts at: 444 504 2853 3109 444
Size: 60 2349 256 835
Fragments arranged by size:
2349 835 256 60
AseI AT'TA_AT
Cuts at: 20 307 20
Size: 287 3213
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3500
AvaI C'yCGr_G
Cuts at: 520 520
Size: 3500
BamHI G'GATC_C
Cuts at: 525 525
Size: 3500
BanI G'GyrC_C
Cuts at: 516 930 1178 1213 516
Size: 414 248 35 2803
Fragments arranged by size:
2803 414 248 35
BanII G_rGCy'C
Cuts at: 248 514 1544 248
Size: 266 1030 2204
Fragments arranged by size:
2204 1030 266
BbeI G_GCGC'C
Cuts at: 934 1182 934
Size: 248 3252
BbsI GAAGACnn'nnnn_
Cuts at: 495 495
Size: 3500
BbvCI CC'TCA_GC
Cuts at: 290 290
Size: 3500
BciVI GTATCCnnnnn_n'
Cuts at: 1398 2159 3500 1398
Size: 761 1341 1398
Fragments arranged by size:
1398 1341 761
BclI T'GATC_A
Cuts at: 1020 1020
Size: 3500
BglII A'GATC_T
Cuts at: 383 1015 3052 383
Size: 632 2037 831
Fragments arranged by size:
2037 831 632
BlpI GC'TnA_GC
Cuts at: 612 612
Size: 3500
Bme1580I G_kGCm'C
Cuts at: 1125 1218 2274 1125
Size: 93 1056 2351
Fragments arranged by size:
2351 1056 93
BmgBI CAC'GTC
Cuts at: 493 493
Size: 3500
BmrI ACTGGGnnnn_n'
Cuts at: 712 793 1128 3272 712
Size: 81 335 2144 940
Fragments arranged by size:
2144 940 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3500
Bpu10I CC'TnA_GC
Cuts at: 290 290
Size: 3500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1555 2047 2345 676
Size: 879 492 298 1831
Fragments arranged by size:
1831 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 273 3204 273
Size: 2931 569
BsaAI yAC'GTr
Cuts at: 853 853
Size: 3500
BsaBI GATnn'nnATC
Cuts at: 6 1014 3051 6
Size: 1008 2037 455
Fragments arranged by size:
2037 1008 455
BsaHI Gr'CG_yC
Cuts at: 931 1179 1881 3377 931
Size: 248 702 1496 1054
Fragments arranged by size:
1496 1054 702 248
BsaWI w'CCGG_w
Cuts at: 689 1210 1888 2162 2309 2720 689
Size: 521 678 274 147 411 1469
Fragments arranged by size:
1469 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 925 1683 2260 2756 925
Size: 758 577 496 1669
Fragments arranged by size:
1669 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1042 1088 2296 104
Size: 938 46 1208 1308
Fragments arranged by size:
1308 1208 938 46
BsiHKAI G_wGCw'C
Cuts at: 514 1292 1482 2274 514
Size: 778 190 792 1740
Fragments arranged by size:
1740 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 248 514 1125 1218 1292 1482 1544 2274 248
Size: 266 611 93 74 190 62 730 1474
Fragments arranged by size:
1474 730 611 266 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2720 2720
Size: 3500
BspHI T'CATG_A
Cuts at: 2676 2676
Size: 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 550 764 1066 1447 550
Size: 214 302 381 2603
Fragments arranged by size:
2603 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1792 1846 3330 3495 50
Size: 1742 54 1484 165 55
Fragments arranged by size:
1742 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 476 1412 476
Size: 936 2564
BsrFI r'CCGG_y
Cuts at: 1498 1679 1888 1498
Size: 181 209 3110
Fragments arranged by size:
3110 209 181
BssSI C'ACGA_G
Cuts at: 118 1771 2129 118
Size: 1653 358 1489
Fragments arranged by size:
1653 1489 358
BstAPI GCAn_nnn'nTGC
Cuts at: 261 877 261
Size: 616 2884
BstBI TT'CG_AA
Cuts at: 1861 1861
Size: 3500
BstYI r'GATC_y
Cuts at: 1 383 525 992 1015 1350 1596 2608 2694
Size: 382 142 467 23 335 246 1012 86
Cuts at: 2694 2706 3052 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 382 346 335 246 142
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1450 1631 1794 1450
Size: 181 163 3156
Fragments arranged by size:
3156 181 163
BtsI GCAGTG_nn'
Cuts at: 743 743
Size: 3500
DraI TTT'AAA
Cuts at: 443 499 443
Size: 56 3444
DrdI GACnn_nn'nnGTC
Cuts at: 1206 2064 1206
Size: 858 2642
EaeI y'GGCC_r
Cuts at: 378 1085 1259 1650 1677 3022 378
Size: 707 174 391 27 1345 856
Fragments arranged by size:
1345 856 707 391 174 27
EagI C'GGCC_G
Cuts at: 1085 1085
Size: 3500
EarI CTCTTCn'nnn_
Cuts at: 1523 1733 1523
Size: 210 3290
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1708 2018 2164 3310 1708
Size: 310 146 1146 1898
Fragments arranged by size:
1898 1146 310 146
EcoICRI GAG'CTC
Cuts at: 512 512
Size: 3500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 1757 2504 2772 1325
Size: 432 747 268 2053
Fragments arranged by size:
2053 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 358 358
Size: 3500
EcoO109I rG'GnC_Cy
Cuts at: 639 639
Size: 3500
EcoRI G'AATT_C
Cuts at: 504 504
Size: 3500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1519 1519
Size: 3500
FspI TGC'GCA
Cuts at: 1281 1281
Size: 3500
HaeII r_GCGC'y
Cuts at: 934 1182 2204 3183 934
Size: 248 1022 979 1251
Fragments arranged by size:
1251 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1342 1372 1374 1404 1342
Size: 30 2 30 3438
Fragments arranged by size:
3438 30 30 2
HincII GTy'rAC
Cuts at: 539 539
Size: 3500
KasI G'GCGC_C
Cuts at: 930 1178 930
Size: 248 3252
KpnI G_GTAC'C
Cuts at: 520 520
Size: 3500
MfeI C'AATT_G
Cuts at: 335 335
Size: 3500
MluI A'CGCG_T
Cuts at: 2933 2933
Size: 3500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 399 2171 2355 227
Size: 172 1772 184 1372
Fragments arranged by size:
1772 1372 184 172
MscI TGG'CCA
Cuts at: 380 1261 380
Size: 881 2619
MslI CAynn'nnrTG
Cuts at: 1489 1616 1489
Size: 127 3373
MspA1I CmG'CkG
Cuts at: 156 611 925 1285 2298 2543 156
Size: 455 314 360 1013 245 1113
Fragments arranged by size:
1113 1013 455 360 314 245
NaeI GCC'GGC
Cuts at: 1681 1681
Size: 3500
NarI GG'CG_CC
Cuts at: 931 1179 931
Size: 248 3252
NgoMIV G'CCGG_C
Cuts at: 1679 1679
Size: 3500
NspI r_CATG'y
Cuts at: 553 1053 1960 553
Size: 500 907 2093
Fragments arranged by size:
2093 907 500
PciI A'CATG_T
Cuts at: 1956 1956
Size: 3500
PmeI GTTT'AAAC
Cuts at: 499 499
Size: 3500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2639 2671 2639
Size: 32 3468
PstI C_TGCA'G
Cuts at: 266 547 1232 266
Size: 281 685 2534
Fragments arranged by size:
2534 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3500
PvuII CAG'CTG
Cuts at: 156 925 1285 156
Size: 769 360 2371
Fragments arranged by size:
2371 769 360
RsrII CG'GwC_CG
Cuts at: 1695 1695
Size: 3500
SacI G_AGCT'C
Cuts at: 514 514
Size: 3500
SalI G'TCGA_C
Cuts at: 537 537
Size: 3500
SapI GCTCTTCn'nnn_
Cuts at: 1523 1733 1523
Size: 210 3290
SbfI CC_TGCA'GG
Cuts at: 547 547
Size: 3500
ScaI AGT'ACT
Cuts at: 3017 3017
Size: 3500
SfcI C'TryA_G
Cuts at: 33 262 543 1228 2221 2412 33
Size: 229 281 685 993 191 1121
Fragments arranged by size:
1121 993 685 281 229 191
SfoI GGC'GCC
Cuts at: 932 1180 932
Size: 248 3252
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3500
SmaI CCC'GGG
Cuts at: 522 522
Size: 3500
SmlI C'TyrA_G
Cuts at: 655 1570 2062 2324 655
Size: 915 492 262 1831
Fragments arranged by size:
1831 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3500
SphI G_CATG'C
Cuts at: 553 1053 553
Size: 500 3000
StyI C'CwwG_G
Cuts at: 125 634 988 125
Size: 509 354 2637
Fragments arranged by size:
2637 509 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1083 1216 1675 1884 1083
Size: 133 459 209 2699
Fragments arranged by size:
2699 459 209 133
TatI w'GTAC_w
Cuts at: 3015 3015
Size: 3500
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 3468
Tth111I GACn'n_nGTC
Cuts at: 1297 1297
Size: 3500
XbaI T'CTAG_A
Cuts at: 531 531
Size: 3500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 995 995
Size: 3500
XmaI C'CCGG_G
Cuts at: 520 520
Size: 3500
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI