Restriction map for
pF1KB6708
(Circular) (Six-base) MAPSORT of: pF1KB6708.seq Check: 1082 from: 1 to: 3500
pF1KB6708 3500 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 538 538
Size: 3500
Acc65I G'GTAC_C
Cuts at: 516 516
Size: 3500
AclI AA'CG_TT
Cuts at: 3340 3340
Size: 3500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 1325 1757 2504 2772 302
Size: 1023 432 747 268 1030
Fragments arranged by size:
1030 1023 747 432 268
AflIII A'CryG_T
Cuts at: 492 852 1956 2933 492
Size: 360 1104 977 1059
Fragments arranged by size:
1104 1059 977 360
AgeI A'CCGG_T
Cuts at: 1888 1888
Size: 3500
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 200 232 200
Size: 32 3468
AlwNI CAG_nnn'CTG
Cuts at: 156 486 805 2372 156
Size: 330 319 1567 1284
Fragments arranged by size:
1567 1284 330 319
ApaLI G'TGCA_C
Cuts at: 2270 2270
Size: 3500
ApoI r'AATT_y
Cuts at: 504 2853 3109 504
Size: 2349 256 895
Fragments arranged by size:
2349 895 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3500
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3500
AvaI C'yCGr_G
Cuts at: 520 520
Size: 3500
BamHI G'GATC_C
Cuts at: 525 525
Size: 3500
BanI G'GyrC_C
Cuts at: 322 474 516 930 1178 1213 322
Size: 152 42 414 248 35 2609
Fragments arranged by size:
2609 414 248 152 42 35
BanII G_rGCy'C
Cuts at: 165 462 514 1544 165
Size: 297 52 1030 2121
Fragments arranged by size:
2121 1030 297 52
BbeI G_GCGC'C
Cuts at: 934 1182 934
Size: 248 3252
BbsI GAAGACnn'nnnn_
Cuts at: 338 338
Size: 3500
BciVI GTATCCnnnnn_n'
Cuts at: 1398 2159 3500 1398
Size: 761 1341 1398
Fragments arranged by size:
1398 1341 761
BclI T'GATC_A
Cuts at: 1020 1020
Size: 3500
BglI GCCn_nnn'nGGC
Cuts at: 151 160 176 151
Size: 9 16 3475
Fragments arranged by size:
3475 16 9
BglII A'GATC_T
Cuts at: 1015 3052 1015
Size: 2037 1463
BlpI GC'TnA_GC
Cuts at: 612 612
Size: 3500
Bme1580I G_kGCm'C
Cuts at: 479 1125 1218 2274 479
Size: 646 93 1056 1705
Fragments arranged by size:
1705 1056 646 93
BmrI ACTGGGnnnn_n'
Cuts at: 167 251 712 793 1128 3272 167
Size: 84 461 81 335 2144 395
Fragments arranged by size:
2144 461 395 335 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 227
Size: 3500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1555 2047 2345 676
Size: 879 492 298 1831
Fragments arranged by size:
1831 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3204 3204
Size: 3500
BsaAI yAC'GTr
Cuts at: 853 853
Size: 3500
BsaBI GATnn'nnATC
Cuts at: 6 1014 3051 6
Size: 1008 2037 455
Fragments arranged by size:
2037 1008 455
BsaHI Gr'CG_yC
Cuts at: 931 1179 1881 3377 931
Size: 248 702 1496 1054
Fragments arranged by size:
1496 1054 702 248
BsaWI w'CCGG_w
Cuts at: 689 1210 1888 2162 2309 2720 689
Size: 521 678 274 147 411 1469
Fragments arranged by size:
1469 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 200 230 200
Size: 30 3470
BseYI C'CCAG_C
Cuts at: 437 479 925 1683 2260 2756 437
Size: 42 446 758 577 496 1181
Fragments arranged by size:
1181 758 577 496 446 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 406
Size: 3500
BsiEI CG_ry'CG
Cuts at: 104 185 1042 1088 2296 104
Size: 81 857 46 1208 1308
Fragments arranged by size:
1308 1208 857 81 46
BsiHKAI G_wGCw'C
Cuts at: 514 1292 1482 2274 514
Size: 778 190 792 1740
Fragments arranged by size:
1740 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 165 462 479 514 1125 1218 1292 1482 1544
Size: 297 17 35 611 93 74 190 62
Cuts at: 1544 2274 165
Size: 730 1391
Fragments arranged by size:
1391 730 611 297 190 93 74 62
35 17
BspEI T'CCGG_A
Cuts at: 2720 2720
Size: 3500
BspHI T'CATG_A
Cuts at: 2676 2676
Size: 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 550 764 1066 1447 550
Size: 214 302 381 2603
Fragments arranged by size:
2603 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1792 1846 3330 3495 50
Size: 1742 54 1484 165 55
Fragments arranged by size:
1742 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1412 1412
Size: 3500
BsrFI r'CCGG_y
Cuts at: 1498 1679 1888 1498
Size: 181 209 3110
Fragments arranged by size:
3110 209 181
BssSI C'ACGA_G
Cuts at: 1771 2129 1771
Size: 358 3142
BstAPI GCAn_nnn'nTGC
Cuts at: 877 877
Size: 3500
BstBI TT'CG_AA
Cuts at: 1861 1861
Size: 3500
BstYI r'GATC_y
Cuts at: 1 525 992 1015 1350 1596 2608 2694 2706
Size: 524 467 23 335 246 1012 86 12
Cuts at: 2706 3052 1
Size: 346 449
Fragments arranged by size:
1012 524 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 157 157
Size: 3500
BtgI C'CryG_G
Cuts at: 107 197 107
Size: 90 3410
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 202 456 1450 1631 1794 202
Size: 254 994 181 163 1908
Fragments arranged by size:
1908 994 254 181 163
BtsI GCAGTG_nn'
Cuts at: 743 743
Size: 3500
DraI TTT'AAA
Cuts at: 499 499
Size: 3500
DrdI GACnn_nn'nnGTC
Cuts at: 1206 2064 1206
Size: 858 2642
EaeI y'GGCC_r
Cuts at: 143 182 1085 1259 1650 1677 3022 143
Size: 39 903 174 391 27 1345 621
Fragments arranged by size:
1345 903 621 391 174 39 27
EagI C'GGCC_G
Cuts at: 182 1085 182
Size: 903 2597
EarI CTCTTCn'nnn_
Cuts at: 224 383 1523 1733 224
Size: 159 1140 210 1991
Fragments arranged by size:
1991 1140 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1708 2018 2164 3310 1708
Size: 310 146 1146 1898
Fragments arranged by size:
1898 1146 310 146
EcoICRI GAG'CTC
Cuts at: 512 512
Size: 3500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 302 1325 1757 2504 2772 227
Size: 75 1023 432 747 268 955
Fragments arranged by size:
1023 955 747 432 268 75
EcoO109I rG'GnC_Cy
Cuts at: 153 639 153
Size: 486 3014
EcoRI G'AATT_C
Cuts at: 504 504
Size: 3500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1519 1519
Size: 3500
FspI TGC'GCA
Cuts at: 1281 1281
Size: 3500
HaeII r_GCGC'y
Cuts at: 934 1182 2204 3183 934
Size: 248 1022 979 1251
Fragments arranged by size:
1251 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1342 1372 1374 1404 1342
Size: 30 2 30 3438
Fragments arranged by size:
3438 30 30 2
HincII GTy'rAC
Cuts at: 539 539
Size: 3500
KasI G'GCGC_C
Cuts at: 930 1178 930
Size: 248 3252
KpnI G_GTAC'C
Cuts at: 520 520
Size: 3500
MluI A'CGCG_T
Cuts at: 2933 2933
Size: 3500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 383 2171 2355 227
Size: 156 1788 184 1372
Fragments arranged by size:
1788 1372 184 156
MscI TGG'CCA
Cuts at: 1261 1261
Size: 3500
MslI CAynn'nnrTG
Cuts at: 1489 1616 1489
Size: 127 3373
MspA1I CmG'CkG
Cuts at: 452 486 611 925 1285 2298 2543 452
Size: 34 125 314 360 1013 245 1409
Fragments arranged by size:
1409 1013 360 314 245 125 34
NaeI GCC'GGC
Cuts at: 1681 1681
Size: 3500
NarI GG'CG_CC
Cuts at: 931 1179 931
Size: 248 3252
NcoI C'CATG_G
Cuts at: 107 197 107
Size: 90 3410
NgoMIV G'CCGG_C
Cuts at: 1679 1679
Size: 3500
NotI GC'GGCC_GC
Cuts at: 182 182
Size: 3500
NspI r_CATG'y
Cuts at: 553 1053 1960 553
Size: 500 907 2093
Fragments arranged by size:
2093 907 500
PciI A'CATG_T
Cuts at: 1956 1956
Size: 3500
PflMI CCAn_nnn'nTGG
Cuts at: 486 486
Size: 3500
PmeI GTTT'AAAC
Cuts at: 499 499
Size: 3500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 200 232 2639 2671 200
Size: 32 2407 32 1029
Fragments arranged by size:
2407 1029 32 32
PstI C_TGCA'G
Cuts at: 547 1232 547
Size: 685 2815
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3500
PvuII CAG'CTG
Cuts at: 486 925 1285 486
Size: 439 360 2701
Fragments arranged by size:
2701 439 360
RsrII CG'GwC_CG
Cuts at: 1695 1695
Size: 3500
SacI G_AGCT'C
Cuts at: 514 514
Size: 3500
SalI G'TCGA_C
Cuts at: 537 537
Size: 3500
SapI GCTCTTCn'nnn_
Cuts at: 224 1523 1733 224
Size: 1299 210 1991
Fragments arranged by size:
1991 1299 210
SbfI CC_TGCA'GG
Cuts at: 547 547
Size: 3500
ScaI AGT'ACT
Cuts at: 3017 3017
Size: 3500
SfcI C'TryA_G
Cuts at: 33 543 1228 2221 2412 33
Size: 510 685 993 191 1121
Fragments arranged by size:
1121 993 685 510 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 151 151
Size: 3500
SfoI GGC'GCC
Cuts at: 932 1180 932
Size: 248 3252
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3500
SmaI CCC'GGG
Cuts at: 522 522
Size: 3500
SmlI C'TyrA_G
Cuts at: 655 1570 2062 2324 655
Size: 915 492 262 1831
Fragments arranged by size:
1831 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3500
SphI G_CATG'C
Cuts at: 553 1053 553
Size: 500 3000
StyI C'CwwG_G
Cuts at: 107 197 634 988 107
Size: 90 437 354 2619
Fragments arranged by size:
2619 437 354 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1083 1216 1675 1884 1083
Size: 133 459 209 2699
Fragments arranged by size:
2699 459 209 133
TatI w'GTAC_w
Cuts at: 297 3015 297
Size: 2718 782
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 3468
Tth111I GACn'n_nGTC
Cuts at: 1297 1297
Size: 3500
XbaI T'CTAG_A
Cuts at: 531 531
Size: 3500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 995 995
Size: 3500
XmaI C'CCGG_G
Cuts at: 520 520
Size: 3500
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI