Restriction map for
pF1KB6710
(Circular) (Six-base) MAPSORT of: pF1KB6710.seq Check: 5327 from: 1 to: 3503
pF1KB6710 3503 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 541 541
Size: 3503
Acc65I G'GTAC_C
Cuts at: 519 519
Size: 3503
AclI AA'CG_TT
Cuts at: 3343 3343
Size: 3503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1328 1760 2507 2775 1328
Size: 432 747 268 2056
Fragments arranged by size:
2056 747 432 268
AflIII A'CryG_T
Cuts at: 855 1959 2936 855
Size: 1104 977 1422
Fragments arranged by size:
1422 1104 977
AgeI A'CCGG_T
Cuts at: 1891 1891
Size: 3503
AlwNI CAG_nnn'CTG
Cuts at: 808 2375 808
Size: 1567 1936
ApaLI G'TGCA_C
Cuts at: 2273 2273
Size: 3503
ApoI r'AATT_y
Cuts at: 253 507 2856 3112 253
Size: 254 2349 256 644
Fragments arranged by size:
2349 644 256 254
AseI AT'TA_AT
Cuts at: 20 20
Size: 3503
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3503
AvaI C'yCGr_G
Cuts at: 523 523
Size: 3503
BamHI G'GATC_C
Cuts at: 528 528
Size: 3503
BanI G'GyrC_C
Cuts at: 519 933 1181 1216 519
Size: 414 248 35 2806
Fragments arranged by size:
2806 414 248 35
BanII G_rGCy'C
Cuts at: 517 1547 517
Size: 1030 2473
BbeI G_GCGC'C
Cuts at: 937 1185 937
Size: 248 3255
BbvCI CC'TCA_GC
Cuts at: 426 426
Size: 3503
BciVI GTATCCnnnnn_n'
Cuts at: 1401 2162 3503 1401
Size: 761 1341 1401
Fragments arranged by size:
1401 1341 761
BclI T'GATC_A
Cuts at: 329 1023 329
Size: 694 2809
BglII A'GATC_T
Cuts at: 1018 3055 1018
Size: 2037 1466
BlpI GC'TnA_GC
Cuts at: 615 615
Size: 3503
Bme1580I G_kGCm'C
Cuts at: 1128 1221 2277 1128
Size: 93 1056 2354
Fragments arranged by size:
2354 1056 93
BmgBI CAC'GTC
Cuts at: 348 348
Size: 3503
BmrI ACTGGGnnnn_n'
Cuts at: 715 796 1131 3275 715
Size: 81 335 2144 943
Fragments arranged by size:
2144 943 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1870 1870
Size: 3503
Bpu10I CC'TnA_GC
Cuts at: 426 426
Size: 3503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 679 1558 2050 2348 124
Size: 555 879 492 298 1279
Fragments arranged by size:
1279 879 555 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3207 3207
Size: 3503
BsaAI yAC'GTr
Cuts at: 856 856
Size: 3503
BsaBI GATnn'nnATC
Cuts at: 6 1017 3054 6
Size: 1011 2037 455
Fragments arranged by size:
2037 1011 455
BsaHI Gr'CG_yC
Cuts at: 934 1182 1884 3380 934
Size: 248 702 1496 1057
Fragments arranged by size:
1496 1057 702 248
BsaWI w'CCGG_w
Cuts at: 212 692 1213 1891 2165 2312 2723 212
Size: 480 521 678 274 147 411 992
Fragments arranged by size:
992 678 521 480 411 274 147
BseYI C'CCAG_C
Cuts at: 280 928 1686 2263 2759 280
Size: 648 758 577 496 1024
Fragments arranged by size:
1024 758 648 577 496
BsiEI CG_ry'CG
Cuts at: 104 1045 1091 2299 104
Size: 941 46 1208 1308
Fragments arranged by size:
1308 1208 941 46
BsiHKAI G_wGCw'C
Cuts at: 199 517 1295 1485 2277 199
Size: 318 778 190 792 1425
Fragments arranged by size:
1425 792 778 318 190
Bsp1286I G_dGCh'C
Cuts at: 199 517 1128 1221 1295 1485 1547 2277 199
Size: 318 611 93 74 190 62 730 1425
Fragments arranged by size:
1425 730 611 318 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2723 2723
Size: 3503
BspHI T'CATG_A
Cuts at: 2679 2679
Size: 3503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 553 767 1069 1450 553
Size: 214 302 381 2606
Fragments arranged by size:
2606 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1795 1849 3333 3498 50
Size: 1745 54 1484 165 55
Fragments arranged by size:
1745 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1415 1415
Size: 3503
BsrFI r'CCGG_y
Cuts at: 1501 1682 1891 1501
Size: 181 209 3113
Fragments arranged by size:
3113 209 181
BssSI C'ACGA_G
Cuts at: 1774 2132 1774
Size: 358 3145
BstAPI GCAn_nnn'nTGC
Cuts at: 880 880
Size: 3503
BstBI TT'CG_AA
Cuts at: 1864 1864
Size: 3503
BstXI CCAn_nnnn'nTGG
Cuts at: 280 280
Size: 3503
BstYI r'GATC_y
Cuts at: 1 528 995 1018 1353 1599 2611 2697 2709
Size: 527 467 23 335 246 1012 86 12
Cuts at: 2709 3055 1
Size: 346 449
Fragments arranged by size:
1012 527 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3503
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1453 1634 1797 1453
Size: 181 163 3159
Fragments arranged by size:
3159 181 163
BtsI GCAGTG_nn'
Cuts at: 746 746
Size: 3503
DraI TTT'AAA
Cuts at: 502 502
Size: 3503
DrdI GACnn_nn'nnGTC
Cuts at: 1209 2067 1209
Size: 858 2645
EaeI y'GGCC_r
Cuts at: 1088 1262 1653 1680 3025 1088
Size: 174 391 27 1345 1566
Fragments arranged by size:
1566 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1088 1088
Size: 3503
EarI CTCTTCn'nnn_
Cuts at: 1526 1736 1526
Size: 210 3293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1711 2021 2167 3313 1711
Size: 310 146 1146 1901
Fragments arranged by size:
1901 1146 310 146
EcoICRI GAG'CTC
Cuts at: 515 515
Size: 3503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1328 1760 2507 2775 1328
Size: 432 747 268 2056
Fragments arranged by size:
2056 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 642 642
Size: 3503
EcoRI G'AATT_C
Cuts at: 507 507
Size: 3503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1522 1522
Size: 3503
FspI TGC'GCA
Cuts at: 115 1284 115
Size: 1169 2334
FspAI rTGC'GCAy
Cuts at: 115 115
Size: 3503
HaeII r_GCGC'y
Cuts at: 937 1185 2207 3186 937
Size: 248 1022 979 1254
Fragments arranged by size:
1254 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 464 496 1345 1375 1377 1407 464
Size: 32 849 30 2 30 2560
Fragments arranged by size:
2560 849 32 30 30 2
HincII GTy'rAC
Cuts at: 542 542
Size: 3503
KasI G'GCGC_C
Cuts at: 933 1181 933
Size: 248 3255
KpnI G_GTAC'C
Cuts at: 523 523
Size: 3503
MluI A'CGCG_T
Cuts at: 2936 2936
Size: 3503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2358 2174
Size: 184 3319
MscI TGG'CCA
Cuts at: 1264 1264
Size: 3503
MslI CAynn'nnrTG
Cuts at: 226 1492 1619 226
Size: 1266 127 2110
Fragments arranged by size:
2110 1266 127
MspA1I CmG'CkG
Cuts at: 430 614 928 1288 2301 2546 430
Size: 184 314 360 1013 245 1387
Fragments arranged by size:
1387 1013 360 314 245 184
NaeI GCC'GGC
Cuts at: 1684 1684
Size: 3503
NarI GG'CG_CC
Cuts at: 934 1182 934
Size: 248 3255
NcoI C'CATG_G
Cuts at: 107 107
Size: 3503
NgoMIV G'CCGG_C
Cuts at: 1682 1682
Size: 3503
NspI r_CATG'y
Cuts at: 556 1056 1963 556
Size: 500 907 2096
Fragments arranged by size:
2096 907 500
PciI A'CATG_T
Cuts at: 1959 1959
Size: 3503
PmeI GTTT'AAAC
Cuts at: 502 502
Size: 3503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 2674 2642
Size: 32 3471
PstI C_TGCA'G
Cuts at: 550 1235 550
Size: 685 2818
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3503
PvuII CAG'CTG
Cuts at: 430 928 1288 430
Size: 498 360 2645
Fragments arranged by size:
2645 498 360
RsrII CG'GwC_CG
Cuts at: 1698 1698
Size: 3503
SacI G_AGCT'C
Cuts at: 517 517
Size: 3503
SalI G'TCGA_C
Cuts at: 540 540
Size: 3503
SapI GCTCTTCn'nnn_
Cuts at: 1526 1736 1526
Size: 210 3293
SbfI CC_TGCA'GG
Cuts at: 550 550
Size: 3503
ScaI AGT'ACT
Cuts at: 3020 3020
Size: 3503
SfcI C'TryA_G
Cuts at: 33 546 1231 2224 2415 33
Size: 513 685 993 191 1121
Fragments arranged by size:
1121 993 685 513 191
SfoI GGC'GCC
Cuts at: 935 1183 935
Size: 248 3255
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3503
SmaI CCC'GGG
Cuts at: 525 525
Size: 3503
SmlI C'TyrA_G
Cuts at: 139 658 1573 2065 2327 139
Size: 519 915 492 262 1315
Fragments arranged by size:
1315 915 519 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3503
SphI G_CATG'C
Cuts at: 556 1056 556
Size: 500 3003
StyI C'CwwG_G
Cuts at: 107 637 991 107
Size: 530 354 2619
Fragments arranged by size:
2619 530 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1086 1219 1678 1887 1086
Size: 133 459 209 2702
Fragments arranged by size:
2702 459 209 133
TatI w'GTAC_w
Cuts at: 414 3018 414
Size: 2604 899
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3471
Tth111I GACn'n_nGTC
Cuts at: 1300 1300
Size: 3503
XbaI T'CTAG_A
Cuts at: 534 534
Size: 3503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 998 998
Size: 3503
XmaI C'CCGG_G
Cuts at: 523 523
Size: 3503
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmtI BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI