Restriction map for
pF1KB6713
(Circular) (Six-base) MAPSORT of: pF1KB6713.seq Check: 3291 from: 1 to: 3353
pF1KB6713 3353 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 391 391
Size: 3353
Acc65I G'GTAC_C
Cuts at: 369 369
Size: 3353
AclI AA'CG_TT
Cuts at: 3193 3193
Size: 3353
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 1178 1610 2357 2625 295
Size: 883 432 747 268 1023
Fragments arranged by size:
1023 883 747 432 268
AflIII A'CryG_T
Cuts at: 705 1809 2786 705
Size: 1104 977 1272
Fragments arranged by size:
1272 1104 977
AgeI A'CCGG_T
Cuts at: 1741 1741
Size: 3353
AlwNI CAG_nnn'CTG
Cuts at: 658 2225 658
Size: 1567 1786
ApaLI G'TGCA_C
Cuts at: 2123 2123
Size: 3353
ApoI r'AATT_y
Cuts at: 180 357 2706 2962 180
Size: 177 2349 256 571
Fragments arranged by size:
2349 571 256 177
AseI AT'TA_AT
Cuts at: 20 20
Size: 3353
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3353
AvaI C'yCGr_G
Cuts at: 373 373
Size: 3353
BamHI G'GATC_C
Cuts at: 378 378
Size: 3353
BanI G'GyrC_C
Cuts at: 369 783 1031 1066 369
Size: 414 248 35 2656
Fragments arranged by size:
2656 414 248 35
BanII G_rGCy'C
Cuts at: 367 1397 367
Size: 1030 2323
BbeI G_GCGC'C
Cuts at: 787 1035 787
Size: 248 3105
BbsI GAAGACnn'nnnn_
Cuts at: 106 106
Size: 3353
BciVI GTATCCnnnnn_n'
Cuts at: 1251 2012 3353 1251
Size: 761 1341 1251
Fragments arranged by size:
1341 1251 761
BclI T'GATC_A
Cuts at: 873 873
Size: 3353
BfrBI ATG'CAT
Cuts at: 295 295
Size: 3353
BglI GCCn_nnn'nGGC
Cuts at: 221 221
Size: 3353
BglII A'GATC_T
Cuts at: 868 2905 868
Size: 2037 1316
BlpI GC'TnA_GC
Cuts at: 465 465
Size: 3353
Bme1580I G_kGCm'C
Cuts at: 978 1071 2127 978
Size: 93 1056 2204
Fragments arranged by size:
2204 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 565 646 981 3125 565
Size: 81 335 2144 793
Fragments arranged by size:
2144 793 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1720 1720
Size: 3353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 262 206
Size: 56 3297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1408 1900 2198 529
Size: 879 492 298 1684
Fragments arranged by size:
1684 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3057 3057
Size: 3353
BsaAI yAC'GTr
Cuts at: 706 706
Size: 3353
BsaBI GATnn'nnATC
Cuts at: 6 867 2904 6
Size: 861 2037 455
Fragments arranged by size:
2037 861 455
BsaHI Gr'CG_yC
Cuts at: 784 1032 1734 3230 784
Size: 248 702 1496 907
Fragments arranged by size:
1496 907 702 248
BsaWI w'CCGG_w
Cuts at: 542 1063 1741 2015 2162 2573 542
Size: 521 678 274 147 411 1322
Fragments arranged by size:
1322 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 778 1536 2113 2609 778
Size: 758 577 496 1522
Fragments arranged by size:
1522 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 895 941 2149 104
Size: 791 46 1208 1308
Fragments arranged by size:
1308 1208 791 46
BsiHKAI G_wGCw'C
Cuts at: 367 1145 1335 2127 367
Size: 778 190 792 1593
Fragments arranged by size:
1593 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 333 333
Size: 3353
Bsp1286I G_dGCh'C
Cuts at: 367 978 1071 1145 1335 1397 2127 367
Size: 611 93 74 190 62 730 1593
Fragments arranged by size:
1593 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2573 2573
Size: 3353
BspHI T'CATG_A
Cuts at: 2529 2529
Size: 3353
BspMI ACCTGCnnnn'nnnn_
Cuts at: 403 617 919 1300 403
Size: 214 302 381 2456
Fragments arranged by size:
2456 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1645 1699 3183 3348 50
Size: 1595 54 1484 165 55
Fragments arranged by size:
1595 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1265 1265
Size: 3353
BsrFI r'CCGG_y
Cuts at: 1351 1532 1741 1351
Size: 181 209 2963
Fragments arranged by size:
2963 209 181
BsrGI T'GTAC_A
Cuts at: 320 320
Size: 3353
BssSI C'ACGA_G
Cuts at: 1624 1982 1624
Size: 358 2995
BstAPI GCAn_nnn'nTGC
Cuts at: 730 730
Size: 3353
BstBI TT'CG_AA
Cuts at: 1714 1714
Size: 3353
BstYI r'GATC_y
Cuts at: 1 378 845 868 1203 1449 2461 2547 2559
Size: 377 467 23 335 246 1012 86 12
Cuts at: 2559 2905 1
Size: 346 449
Fragments arranged by size:
1012 467 449 377 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1303 1484 1647 1303
Size: 181 163 3009
Fragments arranged by size:
3009 181 163
BtsI GCAGTG_nn'
Cuts at: 596 596
Size: 3353
DraI TTT'AAA
Cuts at: 352 352
Size: 3353
DrdI GACnn_nn'nnGTC
Cuts at: 1059 1917 1059
Size: 858 2495
EaeI y'GGCC_r
Cuts at: 938 1112 1503 1530 2875 938
Size: 174 391 27 1345 1416
Fragments arranged by size:
1416 1345 391 174 27
EagI C'GGCC_G
Cuts at: 938 938
Size: 3353
EarI CTCTTCn'nnn_
Cuts at: 331 1376 1586 331
Size: 1045 210 2098
Fragments arranged by size:
2098 1045 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1561 1871 2017 3163 1561
Size: 310 146 1146 1751
Fragments arranged by size:
1751 1146 310 146
EcoICRI GAG'CTC
Cuts at: 365 365
Size: 3353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 262 295 1178 1610 2357 2625 206
Size: 56 33 883 432 747 268 934
Fragments arranged by size:
934 883 747 432 268 56 33
EcoO109I rG'GnC_Cy
Cuts at: 204 492 204
Size: 288 3065
EcoRI G'AATT_C
Cuts at: 357 357
Size: 3353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1372 1372
Size: 3353
FspI TGC'GCA
Cuts at: 1134 1134
Size: 3353
HaeII r_GCGC'y
Cuts at: 787 1035 2057 3036 787
Size: 248 1022 979 1104
Fragments arranged by size:
1104 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1195 1225 1227 1257 1195
Size: 30 2 30 3291
Fragments arranged by size:
3291 30 30 2
HincII GTy'rAC
Cuts at: 392 392
Size: 3353
KasI G'GCGC_C
Cuts at: 783 1031 783
Size: 248 3105
KpnI G_GTAC'C
Cuts at: 373 373
Size: 3353
MluI A'CGCG_T
Cuts at: 2786 2786
Size: 3353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2024 2208 2024
Size: 184 3169
MscI TGG'CCA
Cuts at: 1114 1114
Size: 3353
MslI CAynn'nnrTG
Cuts at: 1342 1469 1342
Size: 127 3226
MspA1I CmG'CkG
Cuts at: 464 778 1138 2151 2396 464
Size: 314 360 1013 245 1421
Fragments arranged by size:
1421 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1534 1534
Size: 3353
NarI GG'CG_CC
Cuts at: 784 1032 784
Size: 248 3105
NgoMIV G'CCGG_C
Cuts at: 1532 1532
Size: 3353
NsiI A_TGCA'T
Cuts at: 297 297
Size: 3353
NspI r_CATG'y
Cuts at: 406 906 1813 406
Size: 500 907 1946
Fragments arranged by size:
1946 907 500
PciI A'CATG_T
Cuts at: 1809 1809
Size: 3353
PmeI GTTT'AAAC
Cuts at: 352 352
Size: 3353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2492 2524 2492
Size: 32 3321
PpuMI rG'GwC_Cy
Cuts at: 204 204
Size: 3353
PstI C_TGCA'G
Cuts at: 400 1085 400
Size: 685 2668
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3353
PvuII CAG'CTG
Cuts at: 778 1138 778
Size: 360 2993
RsrII CG'GwC_CG
Cuts at: 1548 1548
Size: 3353
SacI G_AGCT'C
Cuts at: 367 367
Size: 3353
SalI G'TCGA_C
Cuts at: 390 390
Size: 3353
SapI GCTCTTCn'nnn_
Cuts at: 1376 1586 1376
Size: 210 3143
SbfI CC_TGCA'GG
Cuts at: 400 400
Size: 3353
ScaI AGT'ACT
Cuts at: 2870 2870
Size: 3353
SfcI C'TryA_G
Cuts at: 33 396 1081 2074 2265 33
Size: 363 685 993 191 1121
Fragments arranged by size:
1121 993 685 363 191
SfoI GGC'GCC
Cuts at: 785 1033 785
Size: 248 3105
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3353
SmaI CCC'GGG
Cuts at: 375 375
Size: 3353
SmlI C'TyrA_G
Cuts at: 508 1423 1915 2177 508
Size: 915 492 262 1684
Fragments arranged by size:
1684 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3353
SphI G_CATG'C
Cuts at: 406 906 406
Size: 500 2853
StyI C'CwwG_G
Cuts at: 487 841 487
Size: 354 2999
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 936 1069 1528 1737 936
Size: 133 459 209 2552
Fragments arranged by size:
2552 459 209 133
TatI w'GTAC_w
Cuts at: 320 328 2868 320
Size: 8 2540 805
Fragments arranged by size:
2540 805 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 2870 2902 299
Size: 32 2539 32 750
Fragments arranged by size:
2539 750 32 32
Tth111I GACn'n_nGTC
Cuts at: 1150 1150
Size: 3353
XbaI T'CTAG_A
Cuts at: 384 384
Size: 3353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 848 848
Size: 3353
XmaI C'CCGG_G
Cuts at: 373 373
Size: 3353
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI