(Circular) (Six-base) MAPSORT of: pF1KB6715.seq Check: 885 from: 1 to: 3356 pF1KB6715 3356 bp With 182 enzymes: SgfI * April 24, 2009 13:06 .. AccI GT'mk_AC Cuts at: 394 394 Size: 3356 Acc65I G'GTAC_C Cuts at: 372 372 Size: 3356 AclI AA'CG_TT Cuts at: 3196 3196 Size: 3356 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1181 1613 2360 2628 1181 Size: 432 747 268 1909 Fragments arranged by size: 1909 747 432 268 AflIII A'CryG_T Cuts at: 708 1812 2789 708 Size: 1104 977 1275 Fragments arranged by size: 1275 1104 977 AgeI A'CCGG_T Cuts at: 1744 1744 Size: 3356 AlwNI CAG_nnn'CTG Cuts at: 277 661 2228 277 Size: 384 1567 1405 Fragments arranged by size: 1567 1405 384 ApaLI G'TGCA_C Cuts at: 2126 2126 Size: 3356 ApoI r'AATT_y Cuts at: 360 2709 2965 360 Size: 2349 256 751 Fragments arranged by size: 2349 751 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3356 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3356 AvaI C'yCGr_G Cuts at: 376 376 Size: 3356 BamHI G'GATC_C Cuts at: 381 381 Size: 3356 BanI G'GyrC_C Cuts at: 372 786 1034 1069 372 Size: 414 248 35 2659 Fragments arranged by size: 2659 414 248 35 BanII G_rGCy'C Cuts at: 370 1400 370 Size: 1030 2326 BbeI G_GCGC'C Cuts at: 790 1038 790 Size: 248 3108 BbsI GAAGACnn'nnnn_ Cuts at: 130 274 130 Size: 144 3212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3322 BciVI GTATCCnnnnn_n' Cuts at: 1254 2015 3356 1254 Size: 761 1341 1254 Fragments arranged by size: 1341 1254 761 BclI T'GATC_A Cuts at: 876 876 Size: 3356 BglII A'GATC_T Cuts at: 871 2908 871 Size: 2037 1319 BlpI GC'TnA_GC Cuts at: 468 468 Size: 3356 Bme1580I G_kGCm'C Cuts at: 981 1074 2130 981 Size: 93 1056 2207 Fragments arranged by size: 2207 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 568 649 984 3128 568 Size: 81 335 2144 796 Fragments arranged by size: 2144 796 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1723 1723 Size: 3356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 532 1411 1903 2201 532 Size: 879 492 298 1687 Fragments arranged by size: 1687 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3060 3060 Size: 3356 BsaAI yAC'GTr Cuts at: 709 709 Size: 3356 BsaBI GATnn'nnATC Cuts at: 6 870 2907 6 Size: 864 2037 455 Fragments arranged by size: 2037 864 455 BsaHI Gr'CG_yC Cuts at: 787 1035 1737 3233 787 Size: 248 702 1496 910 Fragments arranged by size: 1496 910 702 248 BsaWI w'CCGG_w Cuts at: 133 545 1066 1744 2018 2165 2576 133 Size: 412 521 678 274 147 411 913 Fragments arranged by size: 913 678 521 412 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 214 344 347 214 Size: 130 3 3223 Fragments arranged by size: 3223 130 3 BseYI C'CCAG_C Cuts at: 781 1539 2116 2612 781 Size: 758 577 496 1525 Fragments arranged by size: 1525 758 577 496 BsiEI CG_ry'CG Cuts at: 104 898 944 2152 104 Size: 794 46 1208 1308 Fragments arranged by size: 1308 1208 794 46 BsiHKAI G_wGCw'C Cuts at: 370 1148 1338 2130 370 Size: 778 190 792 1596 Fragments arranged by size: 1596 792 778 190 Bsp1286I G_dGCh'C Cuts at: 370 981 1074 1148 1338 1400 2130 370 Size: 611 93 74 190 62 730 1596 Fragments arranged by size: 1596 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2576 2576 Size: 3356 BspHI T'CATG_A Cuts at: 2532 2532 Size: 3356 BspMI ACCTGCnnnn'nnnn_ Cuts at: 339 406 620 922 1303 339 Size: 67 214 302 381 2392 Fragments arranged by size: 2392 381 302 214 67 BsrBI CCG'CTC Cuts at: 50 1648 1702 3186 3351 50 Size: 1598 54 1484 165 55 Fragments arranged by size: 1598 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1268 1268 Size: 3356 BsrFI r'CCGG_y Cuts at: 1354 1535 1744 1354 Size: 181 209 2966 Fragments arranged by size: 2966 209 181 BssSI C'ACGA_G Cuts at: 1627 1985 1627 Size: 358 2998 BstAPI GCAn_nnn'nTGC Cuts at: 733 733 Size: 3356 BstBI TT'CG_AA Cuts at: 1717 1717 Size: 3356 BstYI r'GATC_y Cuts at: 1 381 848 871 1206 1452 2464 2550 2562 Size: 380 467 23 335 246 1012 86 12 Cuts at: 2562 2908 1 Size: 346 449 Fragments arranged by size: 1012 467 449 380 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1306 1487 1650 1306 Size: 181 163 3012 Fragments arranged by size: 3012 181 163 BtsI GCAGTG_nn' Cuts at: 599 599 Size: 3356 DraI TTT'AAA Cuts at: 355 355 Size: 3356 DraIII CAC_nnn'GTG Cuts at: 315 315 Size: 3356 DrdI GACnn_nn'nnGTC Cuts at: 1062 1920 1062 Size: 858 2498 EaeI y'GGCC_r Cuts at: 941 1115 1506 1533 2878 941 Size: 174 391 27 1345 1419 Fragments arranged by size: 1419 1345 391 174 27 EagI C'GGCC_G Cuts at: 941 941 Size: 3356 EarI CTCTTCn'nnn_ Cuts at: 1379 1589 1379 Size: 210 3146 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1564 1874 2020 3166 1564 Size: 310 146 1146 1754 Fragments arranged by size: 1754 1146 310 146 EcoICRI GAG'CTC Cuts at: 368 368 Size: 3356 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1181 1613 2360 2628 1181 Size: 432 747 268 1909 Fragments arranged by size: 1909 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 495 495 Size: 3356 EcoRI G'AATT_C Cuts at: 360 360 Size: 3356 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1375 1375 Size: 3356 FspI TGC'GCA Cuts at: 1137 1137 Size: 3356 HaeII r_GCGC'y Cuts at: 790 1038 2060 3039 790 Size: 248 1022 979 1107 Fragments arranged by size: 1107 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1198 1228 1230 1260 1198 Size: 30 2 30 3294 Fragments arranged by size: 3294 30 30 2 HincII GTy'rAC Cuts at: 395 395 Size: 3356 KasI G'GCGC_C Cuts at: 786 1034 786 Size: 248 3108 KpnI G_GTAC'C Cuts at: 376 376 Size: 3356 MluI A'CGCG_T Cuts at: 2789 2789 Size: 3356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2027 2211 2027 Size: 184 3172 MscI TGG'CCA Cuts at: 1117 1117 Size: 3356 MslI CAynn'nnrTG Cuts at: 1345 1472 1345 Size: 127 3229 MspA1I CmG'CkG Cuts at: 277 467 781 1141 2154 2399 277 Size: 190 314 360 1013 245 1234 Fragments arranged by size: 1234 1013 360 314 245 190 NaeI GCC'GGC Cuts at: 1537 1537 Size: 3356 NarI GG'CG_CC Cuts at: 787 1035 787 Size: 248 3108 NgoMIV G'CCGG_C Cuts at: 1535 1535 Size: 3356 NspI r_CATG'y Cuts at: 409 909 1816 409 Size: 500 907 1949 Fragments arranged by size: 1949 907 500 PciI A'CATG_T Cuts at: 1812 1812 Size: 3356 PmeI GTTT'AAAC Cuts at: 355 355 Size: 3356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2495 2527 2495 Size: 32 3324 PstI C_TGCA'G Cuts at: 403 1088 403 Size: 685 2671 PvuI CG_AT'CG Cuts at: 104 104 Size: 3356 PvuII CAG'CTG Cuts at: 277 781 1141 277 Size: 504 360 2492 Fragments arranged by size: 2492 504 360 RsrII CG'GwC_CG Cuts at: 1551 1551 Size: 3356 SacI G_AGCT'C Cuts at: 370 370 Size: 3356 SalI G'TCGA_C Cuts at: 393 393 Size: 3356 SapI GCTCTTCn'nnn_ Cuts at: 1379 1589 1379 Size: 210 3146 SbfI CC_TGCA'GG Cuts at: 403 403 Size: 3356 ScaI AGT'ACT Cuts at: 2873 2873 Size: 3356 SexAI A'CCwGG_T Cuts at: 311 311 Size: 3356 SfcI C'TryA_G Cuts at: 33 164 240 399 1084 2077 2268 33 Size: 131 76 159 685 993 191 1121 Fragments arranged by size: 1121 993 685 191 159 131 76 SfoI GGC'GCC Cuts at: 788 1036 788 Size: 248 3108 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3356 SmaI CCC'GGG Cuts at: 378 378 Size: 3356 SmlI C'TyrA_G Cuts at: 511 1426 1918 2180 511 Size: 915 492 262 1687 Fragments arranged by size: 1687 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3356 SphI G_CATG'C Cuts at: 409 909 409 Size: 500 2856 StyI C'CwwG_G Cuts at: 490 844 490 Size: 354 3002 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 939 1072 1531 1740 939 Size: 133 459 209 2555 Fragments arranged by size: 2555 459 209 133 TatI w'GTAC_w Cuts at: 2871 2871 Size: 3356 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2873 2905 2873 Size: 32 3324 Tth111I GACn'n_nGTC Cuts at: 1153 1153 Size: 3356 XbaI T'CTAG_A Cuts at: 387 387 Size: 3356 XcmI CCAnnnn_n'nnnnTGG Cuts at: 851 851 Size: 3356 XmaI C'CCGG_G Cuts at: 376 376 Size: 3356 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI