Restriction map for pF1KB6715
 (Circular) (Six-base) MAPSORT of: pF1KB6715.seq  Check: 885  from: 1  to: 3356



pF1KB6715  3356 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:    394     394 
   Size:       3356
 
Acc65I G'GTAC_C

Cuts at:    372     372 
   Size:       3356
 
AclI AA'CG_TT

Cuts at:   3196    3196 
   Size:       3356
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1181    1613    2360    2628    1181 
   Size:        432     747     268    1909

  Fragments arranged by size: 

                1909     747     432     268
 
AflIII A'CryG_T

Cuts at:    708    1812    2789     708 
   Size:       1104     977    1275

  Fragments arranged by size: 

                1275    1104     977
 
AgeI A'CCGG_T

Cuts at:   1744    1744 
   Size:       3356
 
AlwNI CAG_nnn'CTG

Cuts at:    277     661    2228     277 
   Size:        384    1567    1405

  Fragments arranged by size: 

                1567    1405     384
 
ApaLI G'TGCA_C

Cuts at:   2126    2126 
   Size:       3356
 
ApoI r'AATT_y

Cuts at:    360    2709    2965     360 
   Size:       2349     256     751

  Fragments arranged by size: 

                2349     751     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3356
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3356
 
AvaI C'yCGr_G

Cuts at:    376     376 
   Size:       3356
 
BamHI G'GATC_C

Cuts at:    381     381 
   Size:       3356
 
BanI G'GyrC_C

Cuts at:    372     786    1034    1069     372 
   Size:        414     248      35    2659

  Fragments arranged by size: 

                2659     414     248      35
 
BanII G_rGCy'C

Cuts at:    370    1400     370 
   Size:       1030    2326
 
BbeI G_GCGC'C

Cuts at:    790    1038     790 
   Size:        248    3108
 
BbsI GAAGACnn'nnnn_

Cuts at:    130     274     130 
   Size:        144    3212
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3322
 
BciVI GTATCCnnnnn_n'

Cuts at:   1254    2015    3356    1254 
   Size:        761    1341    1254

  Fragments arranged by size: 

                1341    1254     761
 
BclI T'GATC_A

Cuts at:    876     876 
   Size:       3356
 
BglII A'GATC_T

Cuts at:    871    2908     871 
   Size:       2037    1319
 
BlpI GC'TnA_GC

Cuts at:    468     468 
   Size:       3356
 
Bme1580I G_kGCm'C

Cuts at:    981    1074    2130     981 
   Size:         93    1056    2207

  Fragments arranged by size: 

                2207    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    568     649     984    3128     568 
   Size:         81     335    2144     796

  Fragments arranged by size: 

                2144     796     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1723    1723 
   Size:       3356
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    532    1411    1903    2201     532 
   Size:        879     492     298    1687

  Fragments arranged by size: 

                1687     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3060    3060 
   Size:       3356
 
BsaAI yAC'GTr

Cuts at:    709     709 
   Size:       3356
 
BsaBI GATnn'nnATC

Cuts at:      6     870    2907       6 
   Size:        864    2037     455

  Fragments arranged by size: 

                2037     864     455
 
BsaHI Gr'CG_yC

Cuts at:    787    1035    1737    3233     787 
   Size:        248     702    1496     910

  Fragments arranged by size: 

                1496     910     702     248
 
BsaWI w'CCGG_w

Cuts at:    133     545    1066    1744    2018    2165    2576     133 
   Size:        412     521     678     274     147     411     913

  Fragments arranged by size: 

                 913     678     521     412     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    214     344     347     214 
   Size:        130       3    3223

  Fragments arranged by size: 

                3223     130       3
 
BseYI C'CCAG_C

Cuts at:    781    1539    2116    2612     781 
   Size:        758     577     496    1525

  Fragments arranged by size: 

                1525     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     898     944    2152     104 
   Size:        794      46    1208    1308

  Fragments arranged by size: 

                1308    1208     794      46
 
BsiHKAI G_wGCw'C

Cuts at:    370    1148    1338    2130     370 
   Size:        778     190     792    1596

  Fragments arranged by size: 

                1596     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    370     981    1074    1148    1338    1400    2130     370 
   Size:        611      93      74     190      62     730    1596

  Fragments arranged by size: 

                1596     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2576    2576 
   Size:       3356
 
BspHI T'CATG_A

Cuts at:   2532    2532 
   Size:       3356
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    339     406     620     922    1303     339 
   Size:         67     214     302     381    2392

  Fragments arranged by size: 

                2392     381     302     214      67
 
BsrBI CCG'CTC

Cuts at:     50    1648    1702    3186    3351      50 
   Size:       1598      54    1484     165      55

  Fragments arranged by size: 

                1598    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1268    1268 
   Size:       3356
 
BsrFI r'CCGG_y

Cuts at:   1354    1535    1744    1354 
   Size:        181     209    2966

  Fragments arranged by size: 

                2966     209     181
 
BssSI C'ACGA_G

Cuts at:   1627    1985    1627 
   Size:        358    2998
 
BstAPI GCAn_nnn'nTGC

Cuts at:    733     733 
   Size:       3356
 
BstBI TT'CG_AA

Cuts at:   1717    1717 
   Size:       3356
 
BstYI r'GATC_y

Cuts at:      1     381     848     871    1206    1452    2464    2550    2562 
   Size:        380     467      23     335     246    1012      86      12

Cuts at:   2562    2908       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     449     380     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1306    1487    1650    1306 
   Size:        181     163    3012

  Fragments arranged by size: 

                3012     181     163
 
BtsI GCAGTG_nn'

Cuts at:    599     599 
   Size:       3356
 
DraI TTT'AAA

Cuts at:    355     355 
   Size:       3356
 
DraIII CAC_nnn'GTG

Cuts at:    315     315 
   Size:       3356
 
DrdI GACnn_nn'nnGTC

Cuts at:   1062    1920    1062 
   Size:        858    2498
 
EaeI y'GGCC_r

Cuts at:    941    1115    1506    1533    2878     941 
   Size:        174     391      27    1345    1419

  Fragments arranged by size: 

                1419    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    941     941 
   Size:       3356
 
EarI CTCTTCn'nnn_

Cuts at:   1379    1589    1379 
   Size:        210    3146
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1564    1874    2020    3166    1564 
   Size:        310     146    1146    1754

  Fragments arranged by size: 

                1754    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    368     368 
   Size:       3356
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1181    1613    2360    2628    1181 
   Size:        432     747     268    1909

  Fragments arranged by size: 

                1909     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    495     495 
   Size:       3356
 
EcoRI G'AATT_C

Cuts at:    360     360 
   Size:       3356
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1375    1375 
   Size:       3356
 
FspI TGC'GCA

Cuts at:   1137    1137 
   Size:       3356
 
HaeII r_GCGC'y

Cuts at:    790    1038    2060    3039     790 
   Size:        248    1022     979    1107

  Fragments arranged by size: 

                1107    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1198    1228    1230    1260    1198 
   Size:         30       2      30    3294

  Fragments arranged by size: 

                3294      30      30       2
 
HincII GTy'rAC

Cuts at:    395     395 
   Size:       3356
 
KasI G'GCGC_C

Cuts at:    786    1034     786 
   Size:        248    3108
 
KpnI G_GTAC'C

Cuts at:    376     376 
   Size:       3356
 
MluI A'CGCG_T

Cuts at:   2789    2789 
   Size:       3356
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2027    2211    2027 
   Size:        184    3172
 
MscI TGG'CCA

Cuts at:   1117    1117 
   Size:       3356
 
MslI CAynn'nnrTG

Cuts at:   1345    1472    1345 
   Size:        127    3229
 
MspA1I CmG'CkG

Cuts at:    277     467     781    1141    2154    2399     277 
   Size:        190     314     360    1013     245    1234

  Fragments arranged by size: 

                1234    1013     360     314     245     190
 
NaeI GCC'GGC

Cuts at:   1537    1537 
   Size:       3356
 
NarI GG'CG_CC

Cuts at:    787    1035     787 
   Size:        248    3108
 
NgoMIV G'CCGG_C

Cuts at:   1535    1535 
   Size:       3356
 
NspI r_CATG'y

Cuts at:    409     909    1816     409 
   Size:        500     907    1949

  Fragments arranged by size: 

                1949     907     500
 
PciI A'CATG_T

Cuts at:   1812    1812 
   Size:       3356
 
PmeI GTTT'AAAC

Cuts at:    355     355 
   Size:       3356
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2495    2527    2495 
   Size:         32    3324
 
PstI C_TGCA'G

Cuts at:    403    1088     403 
   Size:        685    2671
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3356
 
PvuII CAG'CTG

Cuts at:    277     781    1141     277 
   Size:        504     360    2492

  Fragments arranged by size: 

                2492     504     360
 
RsrII CG'GwC_CG

Cuts at:   1551    1551 
   Size:       3356
 
SacI G_AGCT'C

Cuts at:    370     370 
   Size:       3356
 
SalI G'TCGA_C

Cuts at:    393     393 
   Size:       3356
 
SapI GCTCTTCn'nnn_

Cuts at:   1379    1589    1379 
   Size:        210    3146
 
SbfI CC_TGCA'GG

Cuts at:    403     403 
   Size:       3356
 
ScaI AGT'ACT

Cuts at:   2873    2873 
   Size:       3356
 
SexAI A'CCwGG_T

Cuts at:    311     311 
   Size:       3356
 
SfcI C'TryA_G

Cuts at:     33     164     240     399    1084    2077    2268      33 
   Size:        131      76     159     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     191     159     131      76
 
SfoI GGC'GCC

Cuts at:    788    1036     788 
   Size:        248    3108
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3356
 
SmaI CCC'GGG

Cuts at:    378     378 
   Size:       3356
 
SmlI C'TyrA_G

Cuts at:    511    1426    1918    2180     511 
   Size:        915     492     262    1687

  Fragments arranged by size: 

                1687     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3356
 
SphI G_CATG'C

Cuts at:    409     909     409 
   Size:        500    2856
 
StyI C'CwwG_G

Cuts at:    490     844     490 
   Size:        354    3002
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    939    1072    1531    1740     939 
   Size:        133     459     209    2555

  Fragments arranged by size: 

                2555     459     209     133
 
TatI w'GTAC_w

Cuts at:   2871    2871 
   Size:       3356
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2873    2905    2873 
   Size:         32    3324
 
Tth111I GACn'n_nGTC

Cuts at:   1153    1153 
   Size:       3356
 
XbaI T'CTAG_A

Cuts at:    387     387 
   Size:       3356
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    851     851 
   Size:       3356
 
XmaI C'CCGG_G

Cuts at:    376     376 
   Size:       3356
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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