Restriction map for
pF1KB6724
(Circular) (Six-base) MAPSORT of: pF1KB6724.seq Check: 7973 from: 1 to: 3518
pF1KB6724 3518 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AatII G_ACGT'C
Cuts at: 185 185
Size: 3518
AccI GT'mk_AC
Cuts at: 116 556 116
Size: 440 3078
Acc65I G'GTAC_C
Cuts at: 534 534
Size: 3518
AclI AA'CG_TT
Cuts at: 3358 3358
Size: 3518
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 1343 1775 2522 2790 238
Size: 1105 432 747 268 966
Fragments arranged by size:
1105 966 747 432 268
AflIII A'CryG_T
Cuts at: 870 1974 2951 870
Size: 1104 977 1437
Fragments arranged by size:
1437 1104 977
AgeI A'CCGG_T
Cuts at: 1906 1906
Size: 3518
AlwNI CAG_nnn'CTG
Cuts at: 823 2390 823
Size: 1567 1951
ApaLI G'TGCA_C
Cuts at: 2288 2288
Size: 3518
ApoI r'AATT_y
Cuts at: 153 522 2871 3127 153
Size: 369 2349 256 544
Fragments arranged by size:
2349 544 369 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3518
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3518
AvaI C'yCGr_G
Cuts at: 538 538
Size: 3518
BamHI G'GATC_C
Cuts at: 543 543
Size: 3518
BanI G'GyrC_C
Cuts at: 534 948 1196 1231 534
Size: 414 248 35 2821
Fragments arranged by size:
2821 414 248 35
BanII G_rGCy'C
Cuts at: 532 1562 532
Size: 1030 2488
BbeI G_GCGC'C
Cuts at: 952 1200 952
Size: 248 3270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3484
BciVI GTATCCnnnnn_n'
Cuts at: 1416 2177 3518 1416
Size: 761 1341 1416
Fragments arranged by size:
1416 1341 761
BclI T'GATC_A
Cuts at: 257 1038 257
Size: 781 2737
BglII A'GATC_T
Cuts at: 1033 3070 1033
Size: 2037 1481
BlpI GC'TnA_GC
Cuts at: 271 630 271
Size: 359 3159
Bme1580I G_kGCm'C
Cuts at: 1143 1236 2292 1143
Size: 93 1056 2369
Fragments arranged by size:
2369 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 134 426 730 811 1146 3290 134
Size: 292 304 81 335 2144 362
Fragments arranged by size:
2144 362 335 304 292 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 403 1885 403
Size: 1482 2036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 481 373
Size: 108 3410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1573 2065 2363 694
Size: 879 492 298 1849
Fragments arranged by size:
1849 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3222 3222
Size: 3518
BsaAI yAC'GTr
Cuts at: 871 871
Size: 3518
BsaBI GATnn'nnATC
Cuts at: 6 1032 3069 6
Size: 1026 2037 455
Fragments arranged by size:
2037 1026 455
BsaHI Gr'CG_yC
Cuts at: 182 949 1197 1899 3395 182
Size: 767 248 702 1496 305
Fragments arranged by size:
1496 767 702 305 248
BsaWI w'CCGG_w
Cuts at: 707 1228 1906 2180 2327 2738 707
Size: 521 678 274 147 411 1487
Fragments arranged by size:
1487 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 337 175
Size: 162 3356
BseYI C'CCAG_C
Cuts at: 371 943 1701 2278 2774 371
Size: 572 758 577 496 1115
Fragments arranged by size:
1115 758 577 572 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 418 439 529 259
Size: 159 21 90 3248
Fragments arranged by size:
3248 159 90 21
BsiEI CG_ry'CG
Cuts at: 104 1060 1106 2314 104
Size: 956 46 1208 1308
Fragments arranged by size:
1308 1208 956 46
BsiHKAI G_wGCw'C
Cuts at: 277 532 1310 1500 2292 277
Size: 255 778 190 792 1503
Fragments arranged by size:
1503 792 778 255 190
Bsp1286I G_dGCh'C
Cuts at: 277 532 1143 1236 1310 1500 1562 2292 277
Size: 255 611 93 74 190 62 730 1503
Fragments arranged by size:
1503 730 611 255 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2738 2738
Size: 3518
BspHI T'CATG_A
Cuts at: 2694 2694
Size: 3518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 501 568 782 1084 1465 176
Size: 325 67 214 302 381 2229
Fragments arranged by size:
2229 381 325 302 214 67
BsrBI CCG'CTC
Cuts at: 50 1810 1864 3348 3513 50
Size: 1760 54 1484 165 55
Fragments arranged by size:
1760 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1430 1430
Size: 3518
BsrFI r'CCGG_y
Cuts at: 1516 1697 1906 1516
Size: 181 209 3128
Fragments arranged by size:
3128 209 181
BssHII G'CGCG_C
Cuts at: 171 171
Size: 3518
BssSI C'ACGA_G
Cuts at: 1789 2147 1789
Size: 358 3160
BstAPI GCAn_nnn'nTGC
Cuts at: 895 895
Size: 3518
BstBI TT'CG_AA
Cuts at: 1879 1879
Size: 3518
BstEII G'GTnAC_C
Cuts at: 247 247
Size: 3518
BstYI r'GATC_y
Cuts at: 1 327 543 1010 1033 1368 1614 2626 2712
Size: 326 216 467 23 335 246 1012 86
Cuts at: 2712 2724 3070 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 326 246 216
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1468 1649 1812 1468
Size: 181 163 3174
Fragments arranged by size:
3174 181 163
BtsI GCAGTG_nn'
Cuts at: 761 761
Size: 3518
DraI TTT'AAA
Cuts at: 517 517
Size: 3518
DrdI GACnn_nn'nnGTC
Cuts at: 1224 2082 1224
Size: 858 2660
EaeI y'GGCC_r
Cuts at: 1103 1277 1668 1695 3040 1103
Size: 174 391 27 1345 1581
Fragments arranged by size:
1581 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1103 1103
Size: 3518
EarI CTCTTCn'nnn_
Cuts at: 1541 1751 1541
Size: 210 3308
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1726 2036 2182 3328 1726
Size: 310 146 1146 1916
Fragments arranged by size:
1916 1146 310 146
EcoICRI GAG'CTC
Cuts at: 530 530
Size: 3518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 373 481 1343 1775 2522 2790 238
Size: 135 108 862 432 747 268 966
Fragments arranged by size:
966 862 747 432 268 135 108
EcoO109I rG'GnC_Cy
Cuts at: 657 657
Size: 3518
EcoRI G'AATT_C
Cuts at: 522 522
Size: 3518
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1537 1537
Size: 3518
FspI TGC'GCA
Cuts at: 199 1299 199
Size: 1100 2418
HaeII r_GCGC'y
Cuts at: 952 1200 2222 3201 952
Size: 248 1022 979 1269
Fragments arranged by size:
1269 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1360 1390 1392 1422 1360
Size: 30 2 30 3456
Fragments arranged by size:
3456 30 30 2
HincII GTy'rAC
Cuts at: 117 147 557 117
Size: 30 410 3078
Fragments arranged by size:
3078 410 30
KasI G'GCGC_C
Cuts at: 948 1196 948
Size: 248 3270
KpnI G_GTAC'C
Cuts at: 538 538
Size: 3518
MluI A'CGCG_T
Cuts at: 2951 2951
Size: 3518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2373 2189
Size: 184 3334
MscI TGG'CCA
Cuts at: 1279 1279
Size: 3518
MslI CAynn'nnrTG
Cuts at: 1507 1634 1507
Size: 127 3391
MspA1I CmG'CkG
Cuts at: 629 943 1303 2316 2561 629
Size: 314 360 1013 245 1586
Fragments arranged by size:
1586 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1699 1699
Size: 3518
NarI GG'CG_CC
Cuts at: 949 1197 949
Size: 248 3270
NdeI CA'TA_TG
Cuts at: 254 254
Size: 3518
NgoMIV G'CCGG_C
Cuts at: 1697 1697
Size: 3518
NspI r_CATG'y
Cuts at: 571 1071 1978 571
Size: 500 907 2111
Fragments arranged by size:
2111 907 500
PciI A'CATG_T
Cuts at: 1974 1974
Size: 3518
PmeI GTTT'AAAC
Cuts at: 517 517
Size: 3518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2657 2689 2657
Size: 32 3486
PstI C_TGCA'G
Cuts at: 565 1250 565
Size: 685 2833
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3518
PvuII CAG'CTG
Cuts at: 943 1303 943
Size: 360 3158
RsrII CG'GwC_CG
Cuts at: 1713 1713
Size: 3518
SacI G_AGCT'C
Cuts at: 532 532
Size: 3518
SalI G'TCGA_C
Cuts at: 115 555 115
Size: 440 3078
SapI GCTCTTCn'nnn_
Cuts at: 1541 1751 1541
Size: 210 3308
SbfI CC_TGCA'GG
Cuts at: 565 565
Size: 3518
ScaI AGT'ACT
Cuts at: 3035 3035
Size: 3518
SfcI C'TryA_G
Cuts at: 33 561 1246 2239 2430 33
Size: 528 685 993 191 1121
Fragments arranged by size:
1121 993 685 528 191
SfoI GGC'GCC
Cuts at: 950 1198 950
Size: 248 3270
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3518
SmaI CCC'GGG
Cuts at: 540 540
Size: 3518
SmlI C'TyrA_G
Cuts at: 673 1588 2080 2342 673
Size: 915 492 262 1849
Fragments arranged by size:
1849 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3518
SphI G_CATG'C
Cuts at: 571 1071 571
Size: 500 3018
StyI C'CwwG_G
Cuts at: 652 1006 652
Size: 354 3164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1101 1234 1693 1902 1101
Size: 133 459 209 2717
Fragments arranged by size:
2717 459 209 133
TatI w'GTAC_w
Cuts at: 3033 3033
Size: 3518
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 429 461 3035 3067 429
Size: 32 2574 32 880
Fragments arranged by size:
2574 880 32 32
Tth111I GACn'n_nGTC
Cuts at: 1315 1315
Size: 3518
XbaI T'CTAG_A
Cuts at: 549 549
Size: 3518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1013 1013
Size: 3518
XmaI C'CCGG_G
Cuts at: 538 538
Size: 3518
ZraI GAC'GTC
Cuts at: 183 183
Size: 3518
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI