Restriction map for
pF1KB6725
(Circular) (Six-base) MAPSORT of: pF1KB6725.seq Check: 8821 from: 1 to: 3389
pF1KB6725 3389 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 427 427
Size: 3389
Acc65I G'GTAC_C
Cuts at: 405 405
Size: 3389
AclI AA'CG_TT
Cuts at: 3229 3229
Size: 3389
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 1214 1646 2393 2661 195
Size: 1019 432 747 268 923
Fragments arranged by size:
1019 923 747 432 268
AflIII A'CryG_T
Cuts at: 741 1845 2822 741
Size: 1104 977 1308
Fragments arranged by size:
1308 1104 977
AgeI A'CCGG_T
Cuts at: 1777 1777
Size: 3389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 341 341
Size: 3389
AlwNI CAG_nnn'CTG
Cuts at: 694 2261 694
Size: 1567 1822
ApaLI G'TGCA_C
Cuts at: 2159 2159
Size: 3389
ApoI r'AATT_y
Cuts at: 240 393 2742 2998 240
Size: 153 2349 256 631
Fragments arranged by size:
2349 631 256 153
AseI AT'TA_AT
Cuts at: 20 20
Size: 3389
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3389
AvaI C'yCGr_G
Cuts at: 409 409
Size: 3389
BamHI G'GATC_C
Cuts at: 414 414
Size: 3389
BanI G'GyrC_C
Cuts at: 405 819 1067 1102 405
Size: 414 248 35 2692
Fragments arranged by size:
2692 414 248 35
BanII G_rGCy'C
Cuts at: 403 1433 403
Size: 1030 2359
BbeI G_GCGC'C
Cuts at: 823 1071 823
Size: 248 3141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3355
BciVI GTATCCnnnnn_n'
Cuts at: 1287 2048 3389 1287
Size: 761 1341 1287
Fragments arranged by size:
1341 1287 761
BclI T'GATC_A
Cuts at: 909 909
Size: 3389
BglII A'GATC_T
Cuts at: 904 2941 904
Size: 2037 1352
BlpI GC'TnA_GC
Cuts at: 501 501
Size: 3389
Bme1580I G_kGCm'C
Cuts at: 1014 1107 2163 1014
Size: 93 1056 2240
Fragments arranged by size:
2240 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 329 601 682 1017 3161 329
Size: 272 81 335 2144 557
Fragments arranged by size:
2144 557 335 272 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1756 1756
Size: 3389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 391
Size: 3389
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1444 1936 2234 565
Size: 879 492 298 1720
Fragments arranged by size:
1720 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 120 3093 120
Size: 2973 416
BsaAI yAC'GTr
Cuts at: 742 742
Size: 3389
BsaBI GATnn'nnATC
Cuts at: 6 903 2940 6
Size: 897 2037 455
Fragments arranged by size:
2037 897 455
BsaHI Gr'CG_yC
Cuts at: 820 1068 1770 3266 820
Size: 248 702 1496 943
Fragments arranged by size:
1496 943 702 248
BsaWI w'CCGG_w
Cuts at: 578 1099 1777 2051 2198 2609 578
Size: 521 678 274 147 411 1358
Fragments arranged by size:
1358 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 355 130
Size: 225 3164
BseYI C'CCAG_C
Cuts at: 814 1572 2149 2645 814
Size: 758 577 496 1558
Fragments arranged by size:
1558 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 129
Size: 3389
BsiEI CG_ry'CG
Cuts at: 104 200 931 977 2185 104
Size: 96 731 46 1208 1308
Fragments arranged by size:
1308 1208 731 96 46
BsiHKAI G_wGCw'C
Cuts at: 280 403 1181 1371 2163 280
Size: 123 778 190 792 1506
Fragments arranged by size:
1506 792 778 190 123
BsmBI CGTCTCn'nnnn_
Cuts at: 256 256
Size: 3389
Bsp1286I G_dGCh'C
Cuts at: 280 403 1014 1107 1181 1371 1433 2163 280
Size: 123 611 93 74 190 62 730 1506
Fragments arranged by size:
1506 730 611 190 123 93 74 62
BspEI T'CCGG_A
Cuts at: 2609 2609
Size: 3389
BspHI T'CATG_A
Cuts at: 2565 2565
Size: 3389
BspMI ACCTGCnnnn'nnnn_
Cuts at: 102 309 439 653 955 1336 102
Size: 207 130 214 302 381 2155
Fragments arranged by size:
2155 381 302 214 207 130
BsrBI CCG'CTC
Cuts at: 50 1681 1735 3219 3384 50
Size: 1631 54 1484 165 55
Fragments arranged by size:
1631 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1301 1301
Size: 3389
BsrFI r'CCGG_y
Cuts at: 314 1387 1568 1777 314
Size: 1073 181 209 1926
Fragments arranged by size:
1926 1073 209 181
BssSI C'ACGA_G
Cuts at: 1660 2018 1660
Size: 358 3031
BstAPI GCAn_nnn'nTGC
Cuts at: 181 275 766 181
Size: 94 491 2804
Fragments arranged by size:
2804 491 94
BstBI TT'CG_AA
Cuts at: 1750 1750
Size: 3389
BstYI r'GATC_y
Cuts at: 1 414 881 904 1239 1485 2497 2583 2595
Size: 413 467 23 335 246 1012 86 12
Cuts at: 2595 2941 1
Size: 346 449
Fragments arranged by size:
1012 467 449 413 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 149 149
Size: 3389
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1339 1520 1683 1339
Size: 181 163 3045
Fragments arranged by size:
3045 181 163
BtsI GCAGTG_nn'
Cuts at: 119 632 119
Size: 513 2876
DraI TTT'AAA
Cuts at: 388 388
Size: 3389
DrdI GACnn_nn'nnGTC
Cuts at: 1095 1953 1095
Size: 858 2531
EaeI y'GGCC_r
Cuts at: 974 1148 1539 1566 2911 974
Size: 174 391 27 1345 1452
Fragments arranged by size:
1452 1345 391 174 27
EagI C'GGCC_G
Cuts at: 974 974
Size: 3389
EarI CTCTTCn'nnn_
Cuts at: 1412 1622 1412
Size: 210 3179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1597 1907 2053 3199 1597
Size: 310 146 1146 1787
Fragments arranged by size:
1787 1146 310 146
EcoICRI GAG'CTC
Cuts at: 401 401
Size: 3389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 391 1214 1646 2393 2661 195
Size: 196 823 432 747 268 923
Fragments arranged by size:
923 823 747 432 268 196
EcoO109I rG'GnC_Cy
Cuts at: 528 528
Size: 3389
EcoRI G'AATT_C
Cuts at: 393 393
Size: 3389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1408 1408
Size: 3389
FspI TGC'GCA
Cuts at: 1170 1170
Size: 3389
HaeII r_GCGC'y
Cuts at: 823 1071 2093 3072 823
Size: 248 1022 979 1140
Fragments arranged by size:
1140 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1231 1261 1263 1293 1231
Size: 30 2 30 3327
Fragments arranged by size:
3327 30 30 2
HincII GTy'rAC
Cuts at: 428 428
Size: 3389
KasI G'GCGC_C
Cuts at: 819 1067 819
Size: 248 3141
KpnI G_GTAC'C
Cuts at: 409 409
Size: 3389
MluI A'CGCG_T
Cuts at: 2822 2822
Size: 3389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2060 2244 2060
Size: 184 3205
MscI TGG'CCA
Cuts at: 1150 1150
Size: 3389
MslI CAynn'nnrTG
Cuts at: 1378 1505 1378
Size: 127 3262
MspA1I CmG'CkG
Cuts at: 500 814 1174 2187 2432 500
Size: 314 360 1013 245 1457
Fragments arranged by size:
1457 1013 360 314 245
NaeI GCC'GGC
Cuts at: 316 1570 316
Size: 1254 2135
NarI GG'CG_CC
Cuts at: 820 1068 820
Size: 248 3141
NcoI C'CATG_G
Cuts at: 149 149
Size: 3389
NgoMIV G'CCGG_C
Cuts at: 314 1568 314
Size: 1254 2135
NspI r_CATG'y
Cuts at: 442 942 1849 442
Size: 500 907 1982
Fragments arranged by size:
1982 907 500
PciI A'CATG_T
Cuts at: 1845 1845
Size: 3389
PmeI GTTT'AAAC
Cuts at: 388 388
Size: 3389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2560 2528
Size: 32 3357
PstI C_TGCA'G
Cuts at: 436 1121 436
Size: 685 2704
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3389
PvuII CAG'CTG
Cuts at: 814 1174 814
Size: 360 3029
RsrII CG'GwC_CG
Cuts at: 1584 1584
Size: 3389
SacI G_AGCT'C
Cuts at: 403 403
Size: 3389
SalI G'TCGA_C
Cuts at: 426 426
Size: 3389
SapI GCTCTTCn'nnn_
Cuts at: 1412 1622 1412
Size: 210 3179
SbfI CC_TGCA'GG
Cuts at: 436 436
Size: 3389
ScaI AGT'ACT
Cuts at: 2906 2906
Size: 3389
SfcI C'TryA_G
Cuts at: 33 432 1117 2110 2301 33
Size: 399 685 993 191 1121
Fragments arranged by size:
1121 993 685 399 191
SfoI GGC'GCC
Cuts at: 821 1069 821
Size: 248 3141
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3389
SmaI CCC'GGG
Cuts at: 411 411
Size: 3389
SmlI C'TyrA_G
Cuts at: 544 1459 1951 2213 544
Size: 915 492 262 1720
Fragments arranged by size:
1720 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3389
SphI G_CATG'C
Cuts at: 442 942 442
Size: 500 2889
StyI C'CwwG_G
Cuts at: 149 523 877 149
Size: 374 354 2661
Fragments arranged by size:
2661 374 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 972 1105 1564 1773 972
Size: 133 459 209 2588
Fragments arranged by size:
2588 459 209 133
TatI w'GTAC_w
Cuts at: 2904 2904
Size: 3389
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 3357
Tth111I GACn'n_nGTC
Cuts at: 1186 1186
Size: 3389
XbaI T'CTAG_A
Cuts at: 420 420
Size: 3389
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 884 884
Size: 3389
XmaI C'CCGG_G
Cuts at: 409 409
Size: 3389
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI