Restriction map for
pF1KB6728
(Circular) (Six-base) MAPSORT of: pF1KB6728.seq Check: 5901 from: 1 to: 3524
pF1KB6728 3524 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 562 562
Size: 3524
Acc65I G'GTAC_C
Cuts at: 540 540
Size: 3524
AclI AA'CG_TT
Cuts at: 3364 3364
Size: 3524
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1349 1781 2528 2796 1349
Size: 432 747 268 2077
Fragments arranged by size:
2077 747 432 268
AflIII A'CryG_T
Cuts at: 876 1980 2957 876
Size: 1104 977 1443
Fragments arranged by size:
1443 1104 977
AgeI A'CCGG_T
Cuts at: 1912 1912
Size: 3524
AlwNI CAG_nnn'CTG
Cuts at: 829 2396 829
Size: 1567 1957
ApaLI G'TGCA_C
Cuts at: 2294 2294
Size: 3524
ApoI r'AATT_y
Cuts at: 528 2877 3133 528
Size: 2349 256 919
Fragments arranged by size:
2349 919 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3524
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3524
AvaI C'yCGr_G
Cuts at: 544 544
Size: 3524
BamHI G'GATC_C
Cuts at: 549 549
Size: 3524
BanI G'GyrC_C
Cuts at: 540 954 1202 1237 540
Size: 414 248 35 2827
Fragments arranged by size:
2827 414 248 35
BanII G_rGCy'C
Cuts at: 194 517 538 1568 194
Size: 323 21 1030 2150
Fragments arranged by size:
2150 1030 323 21
BbeI G_GCGC'C
Cuts at: 958 1206 958
Size: 248 3276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 267 301 267
Size: 34 3490
BciVI GTATCCnnnnn_n'
Cuts at: 1422 2183 3524 1422
Size: 761 1341 1422
Fragments arranged by size:
1422 1341 761
BclI T'GATC_A
Cuts at: 158 1044 158
Size: 886 2638
BglII A'GATC_T
Cuts at: 1039 3076 1039
Size: 2037 1487
BlpI GC'TnA_GC
Cuts at: 376 636 376
Size: 260 3264
Bme1580I G_kGCm'C
Cuts at: 1149 1242 2298 1149
Size: 93 1056 2375
Fragments arranged by size:
2375 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 736 817 1152 3296 736
Size: 81 335 2144 964
Fragments arranged by size:
2144 964 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1891 1891
Size: 3524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 132
Size: 3524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1579 2071 2369 700
Size: 879 492 298 1855
Fragments arranged by size:
1855 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3228 3228
Size: 3524
BsaAI yAC'GTr
Cuts at: 877 877
Size: 3524
BsaBI GATnn'nnATC
Cuts at: 6 1038 3075 6
Size: 1032 2037 455
Fragments arranged by size:
2037 1032 455
BsaHI Gr'CG_yC
Cuts at: 955 1203 1905 3401 955
Size: 248 702 1496 1078
Fragments arranged by size:
1496 1078 702 248
BsaWI w'CCGG_w
Cuts at: 713 1234 1912 2186 2333 2744 713
Size: 521 678 274 147 411 1493
Fragments arranged by size:
1493 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 949 1707 2284 2780 949
Size: 758 577 496 1693
Fragments arranged by size:
1693 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1066 1112 2320 104
Size: 962 46 1208 1308
Fragments arranged by size:
1308 1208 962 46
BsiHKAI G_wGCw'C
Cuts at: 517 538 1316 1506 2298 517
Size: 21 778 190 792 1743
Fragments arranged by size:
1743 792 778 190 21
BsmI GAATG_Cn'
Cuts at: 366 366
Size: 3524
Bsp1286I G_dGCh'C
Cuts at: 194 517 538 1149 1242 1316 1506 1568 2298
Size: 323 21 611 93 74 190 62 730
Cuts at: 2298 194
Size: 1420
Fragments arranged by size:
1420 730 611 323 190 93 74 62
21
BspEI T'CCGG_A
Cuts at: 2744 2744
Size: 3524
BspHI T'CATG_A
Cuts at: 2700 2700
Size: 3524
BspMI ACCTGCnnnn'nnnn_
Cuts at: 431 574 788 1090 1471 431
Size: 143 214 302 381 2484
Fragments arranged by size:
2484 381 302 214 143
BsrBI CCG'CTC
Cuts at: 50 1816 1870 3354 3519 50
Size: 1766 54 1484 165 55
Fragments arranged by size:
1766 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 130 1436 130
Size: 1306 2218
BsrFI r'CCGG_y
Cuts at: 264 1522 1703 1912 264
Size: 1258 181 209 1876
Fragments arranged by size:
1876 1258 209 181
BssSI C'ACGA_G
Cuts at: 1795 2153 1795
Size: 358 3166
BstAPI GCAn_nnn'nTGC
Cuts at: 901 901
Size: 3524
BstBI TT'CG_AA
Cuts at: 1885 1885
Size: 3524
BstYI r'GATC_y
Cuts at: 1 307 549 1016 1039 1374 1620 2632 2718
Size: 306 242 467 23 335 246 1012 86
Cuts at: 2718 2730 3076 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 306 246 242
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1474 1655 1818 1474
Size: 181 163 3180
Fragments arranged by size:
3180 181 163
BtsI GCAGTG_nn'
Cuts at: 767 767
Size: 3524
DraI TTT'AAA
Cuts at: 523 523
Size: 3524
DrdI GACnn_nn'nnGTC
Cuts at: 1230 2088 1230
Size: 858 2666
EaeI y'GGCC_r
Cuts at: 312 1109 1283 1674 1701 3046 312
Size: 797 174 391 27 1345 790
Fragments arranged by size:
1345 797 790 391 174 27
EagI C'GGCC_G
Cuts at: 1109 1109
Size: 3524
EarI CTCTTCn'nnn_
Cuts at: 481 1547 1757 481
Size: 1066 210 2248
Fragments arranged by size:
2248 1066 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1732 2042 2188 3334 1732
Size: 310 146 1146 1922
Fragments arranged by size:
1922 1146 310 146
EcoICRI GAG'CTC
Cuts at: 515 536 515
Size: 21 3503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 1349 1781 2528 2796 132
Size: 1217 432 747 268 860
Fragments arranged by size:
1217 860 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 663 663
Size: 3524
EcoRI G'AATT_C
Cuts at: 528 528
Size: 3524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1543 1543
Size: 3524
FspI TGC'GCA
Cuts at: 116 427 463 1305 116
Size: 311 36 842 2335
Fragments arranged by size:
2335 842 311 36
HaeII r_GCGC'y
Cuts at: 455 958 1206 2228 3207 455
Size: 503 248 1022 979 772
Fragments arranged by size:
1022 979 772 503 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 380 412 1366 1396 1398 1428 380
Size: 32 954 30 2 30 2476
Fragments arranged by size:
2476 954 32 30 30 2
HincII GTy'rAC
Cuts at: 563 563
Size: 3524
KasI G'GCGC_C
Cuts at: 954 1202 954
Size: 248 3276
KpnI G_GTAC'C
Cuts at: 544 544
Size: 3524
MluI A'CGCG_T
Cuts at: 2957 2957
Size: 3524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2379 2195
Size: 184 3340
MscI TGG'CCA
Cuts at: 1285 1285
Size: 3524
MslI CAynn'nnrTG
Cuts at: 1513 1640 1513
Size: 127 3397
MspA1I CmG'CkG
Cuts at: 318 635 949 1309 2322 2567 318
Size: 317 314 360 1013 245 1275
Fragments arranged by size:
1275 1013 360 317 314 245
NaeI GCC'GGC
Cuts at: 266 1705 266
Size: 1439 2085
NarI GG'CG_CC
Cuts at: 955 1203 955
Size: 248 3276
NgoMIV G'CCGG_C
Cuts at: 264 1703 264
Size: 1439 2085
NspI r_CATG'y
Cuts at: 577 1077 1984 577
Size: 500 907 2117
Fragments arranged by size:
2117 907 500
PciI A'CATG_T
Cuts at: 1980 1980
Size: 3524
PmeI GTTT'AAAC
Cuts at: 523 523
Size: 3524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2663 2695 2663
Size: 32 3492
PstI C_TGCA'G
Cuts at: 571 1256 571
Size: 685 2839
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3524
PvuII CAG'CTG
Cuts at: 949 1309 949
Size: 360 3164
RsrII CG'GwC_CG
Cuts at: 1719 1719
Size: 3524
SacI G_AGCT'C
Cuts at: 517 538 517
Size: 21 3503
SalI G'TCGA_C
Cuts at: 561 561
Size: 3524
SapI GCTCTTCn'nnn_
Cuts at: 1547 1757 1547
Size: 210 3314
SbfI CC_TGCA'GG
Cuts at: 571 571
Size: 3524
ScaI AGT'ACT
Cuts at: 3041 3041
Size: 3524
SfcI C'TryA_G
Cuts at: 33 180 567 1252 2245 2436 33
Size: 147 387 685 993 191 1121
Fragments arranged by size:
1121 993 685 387 191 147
SfoI GGC'GCC
Cuts at: 956 1204 956
Size: 248 3276
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3524
SmaI CCC'GGG
Cuts at: 546 546
Size: 3524
SmlI C'TyrA_G
Cuts at: 679 1594 2086 2348 679
Size: 915 492 262 1855
Fragments arranged by size:
1855 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3524
SphI G_CATG'C
Cuts at: 577 1077 577
Size: 500 3024
StyI C'CwwG_G
Cuts at: 658 1012 658
Size: 354 3170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1107 1240 1699 1908 1107
Size: 133 459 209 2723
Fragments arranged by size:
2723 459 209 133
TatI w'GTAC_w
Cuts at: 3039 3039
Size: 3524
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3041 3073 3041
Size: 32 3492
Tth111I GACn'n_nGTC
Cuts at: 1321 1321
Size: 3524
XbaI T'CTAG_A
Cuts at: 555 555
Size: 3524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1019 1019
Size: 3524
XmaI C'CCGG_G
Cuts at: 544 544
Size: 3524
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI