Restriction map for
pF1KB6732
(Circular) (Six-base) MAPSORT of: pF1KB6732.seq Check: 266 from: 1 to: 3503
pF1KB6732 3503 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 541 541
Size: 3503
Acc65I G'GTAC_C
Cuts at: 519 519
Size: 3503
AclI AA'CG_TT
Cuts at: 3343 3343
Size: 3503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1328 1760 2507 2775 1328
Size: 432 747 268 2056
Fragments arranged by size:
2056 747 432 268
AflIII A'CryG_T
Cuts at: 855 1959 2936 855
Size: 1104 977 1422
Fragments arranged by size:
1422 1104 977
AgeI A'CCGG_T
Cuts at: 1891 1891
Size: 3503
AhdI GACnn_n'nnGTC
Cuts at: 262 262
Size: 3503
AlwNI CAG_nnn'CTG
Cuts at: 808 2375 808
Size: 1567 1936
ApaLI G'TGCA_C
Cuts at: 2273 2273
Size: 3503
ApoI r'AATT_y
Cuts at: 507 2856 3112 507
Size: 2349 256 898
Fragments arranged by size:
2349 898 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3503
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3503
AvaI C'yCGr_G
Cuts at: 403 523 403
Size: 120 3383
BamHI G'GATC_C
Cuts at: 528 528
Size: 3503
BanI G'GyrC_C
Cuts at: 295 442 519 933 1181 1216 295
Size: 147 77 414 248 35 2582
Fragments arranged by size:
2582 414 248 147 77 35
BanII G_rGCy'C
Cuts at: 202 517 1547 202
Size: 315 1030 2158
Fragments arranged by size:
2158 1030 315
BbeI G_GCGC'C
Cuts at: 299 937 1185 299
Size: 638 248 2617
Fragments arranged by size:
2617 638 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 405 439 90
Size: 34 281 34 3154
Fragments arranged by size:
3154 281 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1401 2162 3503 1401
Size: 761 1341 1401
Fragments arranged by size:
1401 1341 761
BclI T'GATC_A
Cuts at: 1023 1023
Size: 3503
BglII A'GATC_T
Cuts at: 1018 3055 1018
Size: 2037 1466
BlpI GC'TnA_GC
Cuts at: 615 615
Size: 3503
Bme1580I G_kGCm'C
Cuts at: 371 447 1128 1221 2277 371
Size: 76 681 93 1056 1597
Fragments arranged by size:
1597 1056 681 93 76
BmrI ACTGGGnnnn_n'
Cuts at: 715 796 1131 3275 715
Size: 81 335 2144 943
Fragments arranged by size:
2144 943 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 274 1870 274
Size: 1596 1907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1558 2050 2348 679
Size: 879 492 298 1834
Fragments arranged by size:
1834 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3207 3207
Size: 3503
BsaAI yAC'GTr
Cuts at: 856 856
Size: 3503
BsaBI GATnn'nnATC
Cuts at: 6 1017 3054 6
Size: 1011 2037 455
Fragments arranged by size:
2037 1011 455
BsaHI Gr'CG_yC
Cuts at: 296 934 1182 1884 3380 296
Size: 638 248 702 1496 419
Fragments arranged by size:
1496 702 638 419 248
BsaWI w'CCGG_w
Cuts at: 692 1213 1891 2165 2312 2723 692
Size: 521 678 274 147 411 1472
Fragments arranged by size:
1472 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 196 226 196
Size: 30 3473
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 266 499 508 266
Size: 233 9 3261
Fragments arranged by size:
3261 233 9
BseYI C'CCAG_C
Cuts at: 282 928 1686 2263 2759 282
Size: 646 758 577 496 1026
Fragments arranged by size:
1026 758 646 577 496
BsiEI CG_ry'CG
Cuts at: 104 1045 1091 2299 104
Size: 941 46 1208 1308
Fragments arranged by size:
1308 1208 941 46
BsiHKAI G_wGCw'C
Cuts at: 193 202 517 1295 1485 2277 193
Size: 9 315 778 190 792 1419
Fragments arranged by size:
1419 792 778 315 190 9
Bsp1286I G_dGCh'C
Cuts at: 193 202 371 447 517 1128 1221 1295 1485
Size: 9 169 76 70 611 93 74 190
Cuts at: 1485 1547 2277 193
Size: 62 730 1419
Fragments arranged by size:
1419 730 611 190 169 93 76 74
70 62 9
BspEI T'CCGG_A
Cuts at: 2723 2723
Size: 3503
BspHI T'CATG_A
Cuts at: 2679 2679
Size: 3503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 152 166 553 767 1069 1450 152
Size: 14 387 214 302 381 2205
Fragments arranged by size:
2205 387 381 302 214 14
BsrBI CCG'CTC
Cuts at: 50 1795 1849 3333 3498 50
Size: 1745 54 1484 165 55
Fragments arranged by size:
1745 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1415 1415
Size: 3503
BsrFI r'CCGG_y
Cuts at: 292 298 1501 1682 1891 292
Size: 6 1203 181 209 1904
Fragments arranged by size:
1904 1203 209 181 6
BssSI C'ACGA_G
Cuts at: 1774 2132 1774
Size: 358 3145
BstAPI GCAn_nnn'nTGC
Cuts at: 880 880
Size: 3503
BstBI TT'CG_AA
Cuts at: 1864 1864
Size: 3503
BstXI CCAn_nnnn'nTGG
Cuts at: 318 318
Size: 3503
BstYI r'GATC_y
Cuts at: 1 528 995 1018 1353 1599 2611 2697 2709
Size: 527 467 23 335 246 1012 86 12
Cuts at: 2709 3055 1
Size: 346 449
Fragments arranged by size:
1012 527 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 288 1453 1634 1797 288
Size: 1165 181 163 1994
Fragments arranged by size:
1994 1165 181 163
BtsI GCAGTG_nn'
Cuts at: 168 746 168
Size: 578 2925
DraI TTT'AAA
Cuts at: 502 502
Size: 3503
DrdI GACnn_nn'nnGTC
Cuts at: 1209 2067 1209
Size: 858 2645
EaeI y'GGCC_r
Cuts at: 242 308 386 1088 1262 1653 1680 3025 242
Size: 66 78 702 174 391 27 1345 720
Fragments arranged by size:
1345 720 702 391 174 78 66 27
EagI C'GGCC_G
Cuts at: 1088 1088
Size: 3503
EarI CTCTTCn'nnn_
Cuts at: 157 1526 1736 157
Size: 1369 210 1924
Fragments arranged by size:
1924 1369 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1711 2021 2167 3313 1711
Size: 310 146 1146 1901
Fragments arranged by size:
1901 1146 310 146
EcoICRI GAG'CTC
Cuts at: 200 515 200
Size: 315 3188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1328 1760 2507 2775 1328
Size: 432 747 268 2056
Fragments arranged by size:
2056 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 228 642 228
Size: 414 3089
EcoRI G'AATT_C
Cuts at: 507 507
Size: 3503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1522 1522
Size: 3503
FspI TGC'GCA
Cuts at: 1284 1284
Size: 3503
HaeII r_GCGC'y
Cuts at: 299 937 1185 2207 3186 299
Size: 638 248 1022 979 616
Fragments arranged by size:
1022 979 638 616 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 248 280 1345 1375 1377 1407 196
Size: 32 20 32 1065 30 2 30 2292
Fragments arranged by size:
2292 1065 32 32 30 30 20 2
HincII GTy'rAC
Cuts at: 542 542
Size: 3503
KasI G'GCGC_C
Cuts at: 295 933 1181 295
Size: 638 248 2617
Fragments arranged by size:
2617 638 248
KpnI G_GTAC'C
Cuts at: 523 523
Size: 3503
MluI A'CGCG_T
Cuts at: 2936 2936
Size: 3503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 228 2174 2358 228
Size: 1946 184 1373
Fragments arranged by size:
1946 1373 184
MscI TGG'CCA
Cuts at: 388 1264 388
Size: 876 2627
MslI CAynn'nnrTG
Cuts at: 1492 1619 1492
Size: 127 3376
MspA1I CmG'CkG
Cuts at: 209 614 928 1288 2301 2546 209
Size: 405 314 360 1013 245 1166
Fragments arranged by size:
1166 1013 405 360 314 245
NaeI GCC'GGC
Cuts at: 300 1684 300
Size: 1384 2119
NarI GG'CG_CC
Cuts at: 296 934 1182 296
Size: 638 248 2617
Fragments arranged by size:
2617 638 248
NgoMIV G'CCGG_C
Cuts at: 298 1682 298
Size: 1384 2119
NspI r_CATG'y
Cuts at: 556 1056 1963 556
Size: 500 907 2096
Fragments arranged by size:
2096 907 500
PciI A'CATG_T
Cuts at: 1959 1959
Size: 3503
PmeI GTTT'AAAC
Cuts at: 502 502
Size: 3503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 2674 2642
Size: 32 3471
PstI C_TGCA'G
Cuts at: 398 550 1235 398
Size: 152 685 2666
Fragments arranged by size:
2666 685 152
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3503
PvuII CAG'CTG
Cuts at: 928 1288 928
Size: 360 3143
RsrII CG'GwC_CG
Cuts at: 1698 1698
Size: 3503
SacI G_AGCT'C
Cuts at: 202 517 202
Size: 315 3188
SalI G'TCGA_C
Cuts at: 540 540
Size: 3503
SapI GCTCTTCn'nnn_
Cuts at: 157 1526 1736 157
Size: 1369 210 1924
Fragments arranged by size:
1924 1369 210
SbfI CC_TGCA'GG
Cuts at: 550 550
Size: 3503
ScaI AGT'ACT
Cuts at: 3020 3020
Size: 3503
SfcI C'TryA_G
Cuts at: 33 394 546 1231 2224 2415 33
Size: 361 152 685 993 191 1121
Fragments arranged by size:
1121 993 685 361 191 152
SfoI GGC'GCC
Cuts at: 297 935 1183 297
Size: 638 248 2617
Fragments arranged by size:
2617 638 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3503
SgrAI Cr'CCGG_yG
Cuts at: 292 292
Size: 3503
SmaI CCC'GGG
Cuts at: 525 525
Size: 3503
SmlI C'TyrA_G
Cuts at: 658 1573 2065 2327 658
Size: 915 492 262 1834
Fragments arranged by size:
1834 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3503
SphI G_CATG'C
Cuts at: 556 1056 556
Size: 500 3003
SspI AAT'ATT
Cuts at: 438 438
Size: 3503
StyI C'CwwG_G
Cuts at: 637 991 637
Size: 354 3149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 478 1086 1219 1678 1887 478
Size: 608 133 459 209 2094
Fragments arranged by size:
2094 608 459 209 133
TatI w'GTAC_w
Cuts at: 3018 3018
Size: 3503
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3471
Tth111I GACn'n_nGTC
Cuts at: 1300 1300
Size: 3503
XbaI T'CTAG_A
Cuts at: 534 534
Size: 3503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 998 998
Size: 3503
XmaI C'CCGG_G
Cuts at: 523 523
Size: 3503
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI