Restriction map for
pF1KB6734
(Circular) (Six-base) MAPSORT of: pF1KB6734.seq Check: 3114 from: 1 to: 3527
pF1KB6734 3527 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 403 403
Size: 3527
AccI GT'mk_AC
Cuts at: 452 565 452
Size: 113 3414
Acc65I G'GTAC_C
Cuts at: 543 543
Size: 3527
AclI AA'CG_TT
Cuts at: 3367 3367
Size: 3527
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1352 1784 2531 2799 1352
Size: 432 747 268 2080
Fragments arranged by size:
2080 747 432 268
AflIII A'CryG_T
Cuts at: 879 1983 2960 879
Size: 1104 977 1446
Fragments arranged by size:
1446 1104 977
AgeI A'CCGG_T
Cuts at: 1915 1915
Size: 3527
AlwNI CAG_nnn'CTG
Cuts at: 832 2399 832
Size: 1567 1960
ApaI G_GGCC'C
Cuts at: 410 410
Size: 3527
ApaLI G'TGCA_C
Cuts at: 2297 2297
Size: 3527
ApoI r'AATT_y
Cuts at: 531 2880 3136 531
Size: 2349 256 922
Fragments arranged by size:
2349 922 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3527
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3527
AvaI C'yCGr_G
Cuts at: 403 547 403
Size: 144 3383
BamHI G'GATC_C
Cuts at: 552 552
Size: 3527
BanI G'GyrC_C
Cuts at: 283 543 957 1205 1240 283
Size: 260 414 248 35 2570
Fragments arranged by size:
2570 414 260 248 35
BanII G_rGCy'C
Cuts at: 404 410 541 1571 404
Size: 6 131 1030 2360
Fragments arranged by size:
2360 1030 131 6
BbeI G_GCGC'C
Cuts at: 961 1209 961
Size: 248 3279
BciVI GTATCCnnnnn_n'
Cuts at: 1425 2186 3527 1425
Size: 761 1341 1425
Fragments arranged by size:
1425 1341 761
BclI T'GATC_A
Cuts at: 1047 1047
Size: 3527
BglII A'GATC_T
Cuts at: 1042 3079 1042
Size: 2037 1490
BlpI GC'TnA_GC
Cuts at: 639 639
Size: 3527
Bme1580I G_kGCm'C
Cuts at: 410 1152 1245 2301 410
Size: 742 93 1056 1636
Fragments arranged by size:
1636 1056 742 93
BmrI ACTGGGnnnn_n'
Cuts at: 739 820 1155 3299 739
Size: 81 335 2144 967
Fragments arranged by size:
2144 967 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 3527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 703 1582 2074 2372 445
Size: 258 879 492 298 1600
Fragments arranged by size:
1600 879 492 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 3231 3231
Size: 3527
BsaAI yAC'GTr
Cuts at: 880 880
Size: 3527
BsaBI GATnn'nnATC
Cuts at: 6 1041 3078 6
Size: 1035 2037 455
Fragments arranged by size:
2037 1035 455
BsaHI Gr'CG_yC
Cuts at: 958 1206 1908 3404 958
Size: 248 702 1496 1081
Fragments arranged by size:
1496 1081 702 248
BsaWI w'CCGG_w
Cuts at: 440 716 1237 1915 2189 2336 2747 440
Size: 276 521 678 274 147 411 1220
Fragments arranged by size:
1220 678 521 411 276 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 109 346 109
Size: 237 3290
BseYI C'CCAG_C
Cuts at: 254 952 1710 2287 2783 254
Size: 698 758 577 496 998
Fragments arranged by size:
998 758 698 577 496
BsiEI CG_ry'CG
Cuts at: 104 264 1069 1115 2323 104
Size: 160 805 46 1208 1308
Fragments arranged by size:
1308 1208 805 160 46
BsiHKAI G_wGCw'C
Cuts at: 541 1319 1509 2301 541
Size: 778 190 792 1767
Fragments arranged by size:
1767 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 404 410 541 1152 1245 1319 1509 1571 2301
Size: 6 131 611 93 74 190 62 730
Cuts at: 2301 404
Size: 1630
Fragments arranged by size:
1630 730 611 190 131 93 74 62
6
BspEI T'CCGG_A
Cuts at: 2747 2747
Size: 3527
BspHI T'CATG_A
Cuts at: 2703 2703
Size: 3527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 403 577 791 1093 1474 403
Size: 174 214 302 381 2456
Fragments arranged by size:
2456 381 302 214 174
BsrBI CCG'CTC
Cuts at: 50 1819 1873 3357 3522 50
Size: 1769 54 1484 165 55
Fragments arranged by size:
1769 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1439 1439
Size: 3527
BsrFI r'CCGG_y
Cuts at: 1525 1706 1915 1525
Size: 181 209 3137
Fragments arranged by size:
3137 209 181
BssSI C'ACGA_G
Cuts at: 1798 2156 1798
Size: 358 3169
BstAPI GCAn_nnn'nTGC
Cuts at: 904 904
Size: 3527
BstBI TT'CG_AA
Cuts at: 1888 1888
Size: 3527
BstEII G'GTnAC_C
Cuts at: 390 390
Size: 3527
BstXI CCAn_nnnn'nTGG
Cuts at: 131 319 131
Size: 188 3339
BstYI r'GATC_y
Cuts at: 1 552 1019 1042 1377 1623 2635 2721 2733
Size: 551 467 23 335 246 1012 86 12
Cuts at: 2733 3079 1
Size: 346 449
Fragments arranged by size:
1012 551 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1477 1658 1821 1477
Size: 181 163 3183
Fragments arranged by size:
3183 181 163
BtsI GCAGTG_nn'
Cuts at: 312 770 312
Size: 458 3069
DraI TTT'AAA
Cuts at: 526 526
Size: 3527
DrdI GACnn_nn'nnGTC
Cuts at: 1233 2091 1233
Size: 858 2669
EaeI y'GGCC_r
Cuts at: 261 1112 1286 1677 1704 3049 261
Size: 851 174 391 27 1345 739
Fragments arranged by size:
1345 851 739 391 174 27
EagI C'GGCC_G
Cuts at: 261 1112 261
Size: 851 2676
EarI CTCTTCn'nnn_
Cuts at: 1550 1760 1550
Size: 210 3317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1735 2045 2191 3337 1735
Size: 310 146 1146 1925
Fragments arranged by size:
1925 1146 310 146
EcoICRI GAG'CTC
Cuts at: 539 539
Size: 3527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1352 1784 2531 2799 349
Size: 1003 432 747 268 1077
Fragments arranged by size:
1077 1003 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 407 666 407
Size: 259 3268
EcoRI G'AATT_C
Cuts at: 531 531
Size: 3527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1546 1546
Size: 3527
FspI TGC'GCA
Cuts at: 1308 1308
Size: 3527
HaeII r_GCGC'y
Cuts at: 961 1209 2231 3210 961
Size: 248 1022 979 1278
Fragments arranged by size:
1278 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 1369 1399 1401 1431 169
Size: 32 1168 30 2 30 2265
Fragments arranged by size:
2265 1168 32 30 30 2
HincII GTy'rAC
Cuts at: 566 566
Size: 3527
HindIII A'AGCT_T
Cuts at: 445 445
Size: 3527
KasI G'GCGC_C
Cuts at: 957 1205 957
Size: 248 3279
KpnI G_GTAC'C
Cuts at: 547 547
Size: 3527
MluI A'CGCG_T
Cuts at: 2960 2960
Size: 3527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2198 2382 2198
Size: 184 3343
MscI TGG'CCA
Cuts at: 1288 1288
Size: 3527
MslI CAynn'nnrTG
Cuts at: 317 1516 1643 317
Size: 1199 127 2201
Fragments arranged by size:
2201 1199 127
MspA1I CmG'CkG
Cuts at: 638 952 1312 2325 2570 638
Size: 314 360 1013 245 1595
Fragments arranged by size:
1595 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1708 1708
Size: 3527
NarI GG'CG_CC
Cuts at: 958 1206 958
Size: 248 3279
NgoMIV G'CCGG_C
Cuts at: 1706 1706
Size: 3527
NspI r_CATG'y
Cuts at: 580 1080 1987 580
Size: 500 907 2120
Fragments arranged by size:
2120 907 500
PciI A'CATG_T
Cuts at: 1983 1983
Size: 3527
PmeI GTTT'AAAC
Cuts at: 526 526
Size: 3527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2698 2666
Size: 32 3495
PspOMI G'GGCC_C
Cuts at: 406 406
Size: 3527
PstI C_TGCA'G
Cuts at: 574 1259 574
Size: 685 2842
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3527
PvuII CAG'CTG
Cuts at: 952 1312 952
Size: 360 3167
RsrII CG'GwC_CG
Cuts at: 1722 1722
Size: 3527
SacI G_AGCT'C
Cuts at: 541 541
Size: 3527
SalI G'TCGA_C
Cuts at: 564 564
Size: 3527
SapI GCTCTTCn'nnn_
Cuts at: 1550 1760 1550
Size: 210 3317
SbfI CC_TGCA'GG
Cuts at: 574 574
Size: 3527
ScaI AGT'ACT
Cuts at: 3044 3044
Size: 3527
SfcI C'TryA_G
Cuts at: 33 570 1255 2248 2439 33
Size: 537 685 993 191 1121
Fragments arranged by size:
1121 993 685 537 191
SfoI GGC'GCC
Cuts at: 959 1207 959
Size: 248 3279
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3527
SmaI CCC'GGG
Cuts at: 405 549 405
Size: 144 3383
SmlI C'TyrA_G
Cuts at: 460 682 1597 2089 2351 460
Size: 222 915 492 262 1636
Fragments arranged by size:
1636 915 492 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3527
SphI G_CATG'C
Cuts at: 580 1080 580
Size: 500 3027
SrfI GCCC'GGGC
Cuts at: 405 405
Size: 3527
StuI AGG'CCT
Cuts at: 116 116
Size: 3527
StyI C'CwwG_G
Cuts at: 275 661 1015 275
Size: 386 354 2787
Fragments arranged by size:
2787 386 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1110 1243 1702 1911 1110
Size: 133 459 209 2726
Fragments arranged by size:
2726 459 209 133
TatI w'GTAC_w
Cuts at: 3042 3042
Size: 3527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3495
Tth111I GACn'n_nGTC
Cuts at: 1324 1324
Size: 3527
XbaI T'CTAG_A
Cuts at: 558 558
Size: 3527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1022 1022
Size: 3527
XmaI C'CCGG_G
Cuts at: 403 547 403
Size: 144 3383
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI