Restriction map for
pF1KB6738
(Circular) (Six-base) MAPSORT of: pF1KB6738.seq Check: 7210 from: 1 to: 3533
pF1KB6738 3533 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 571 571
Size: 3533
Acc65I G'GTAC_C
Cuts at: 549 549
Size: 3533
AclI AA'CG_TT
Cuts at: 223 3373 223
Size: 3150 383
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1358 1790 2537 2805 1358
Size: 432 747 268 2086
Fragments arranged by size:
2086 747 432 268
AflIII A'CryG_T
Cuts at: 885 1989 2966 885
Size: 1104 977 1452
Fragments arranged by size:
1452 1104 977
AgeI A'CCGG_T
Cuts at: 272 385 1921 272
Size: 113 1536 1884
Fragments arranged by size:
1884 1536 113
AlwNI CAG_nnn'CTG
Cuts at: 838 2405 838
Size: 1567 1966
ApaI G_GGCC'C
Cuts at: 396 396
Size: 3533
ApaLI G'TGCA_C
Cuts at: 2303 2303
Size: 3533
ApoI r'AATT_y
Cuts at: 355 537 2886 3142 355
Size: 182 2349 256 746
Fragments arranged by size:
2349 746 256 182
AseI AT'TA_AT
Cuts at: 20 20
Size: 3533
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
AvaI C'yCGr_G
Cuts at: 214 553 214
Size: 339 3194
BamHI G'GATC_C
Cuts at: 558 558
Size: 3533
BanI G'GyrC_C
Cuts at: 549 963 1211 1246 549
Size: 414 248 35 2836
Fragments arranged by size:
2836 414 248 35
BanII G_rGCy'C
Cuts at: 396 547 1577 396
Size: 151 1030 2352
Fragments arranged by size:
2352 1030 151
BbeI G_GCGC'C
Cuts at: 967 1215 967
Size: 248 3285
BbvCI CC'TCA_GC
Cuts at: 245 245
Size: 3533
BciVI GTATCCnnnnn_n'
Cuts at: 1431 2192 3533 1431
Size: 761 1341 1431
Fragments arranged by size:
1431 1341 761
BclI T'GATC_A
Cuts at: 476 1053 476
Size: 577 2956
BglII A'GATC_T
Cuts at: 1048 3085 1048
Size: 2037 1496
BlpI GC'TnA_GC
Cuts at: 645 645
Size: 3533
Bme1580I G_kGCm'C
Cuts at: 396 1158 1251 2307 396
Size: 762 93 1056 1622
Fragments arranged by size:
1622 1056 762 93
BmrI ACTGGGnnnn_n'
Cuts at: 516 745 826 1161 3305 516
Size: 229 81 335 2144 744
Fragments arranged by size:
2144 744 335 229 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3533
Bpu10I CC'TnA_GC
Cuts at: 245 245
Size: 3533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1588 2080 2378 709
Size: 879 492 298 1864
Fragments arranged by size:
1864 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3237 3237
Size: 3533
BsaAI yAC'GTr
Cuts at: 288 886 288
Size: 598 2935
BsaBI GATnn'nnATC
Cuts at: 6 1047 3084 6
Size: 1041 2037 455
Fragments arranged by size:
2037 1041 455
BsaHI Gr'CG_yC
Cuts at: 964 1212 1914 3410 964
Size: 248 702 1496 1087
Fragments arranged by size:
1496 1087 702 248
BsaWI w'CCGG_w
Cuts at: 272 385 722 1243 1921 2195 2342 2753 272
Size: 113 337 521 678 274 147 411 1052
Fragments arranged by size:
1052 678 521 411 337 274 147 113
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 178 208 178
Size: 30 3503
BseYI C'CCAG_C
Cuts at: 958 1716 2293 2789 958
Size: 758 577 496 1702
Fragments arranged by size:
1702 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1075 1121 2329 104
Size: 971 46 1208 1308
Fragments arranged by size:
1308 1208 971 46
BsiHKAI G_wGCw'C
Cuts at: 385 547 1325 1515 2307 385
Size: 162 778 190 792 1611
Fragments arranged by size:
1611 792 778 190 162
Bsp1286I G_dGCh'C
Cuts at: 385 396 547 1158 1251 1325 1515 1577 2307
Size: 11 151 611 93 74 190 62 730
Cuts at: 2307 385
Size: 1611
Fragments arranged by size:
1611 730 611 190 151 93 74 62
11
BspEI T'CCGG_A
Cuts at: 2753 2753
Size: 3533
BspHI T'CATG_A
Cuts at: 2709 2709
Size: 3533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 583 797 1099 1480 583
Size: 214 302 381 2636
Fragments arranged by size:
2636 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1825 1879 3363 3528 50
Size: 1775 54 1484 165 55
Fragments arranged by size:
1775 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1445 1445
Size: 3533
BsrFI r'CCGG_y
Cuts at: 272 385 1531 1712 1921 272
Size: 113 1146 181 209 1884
Fragments arranged by size:
1884 1146 209 181 113
BssSI C'ACGA_G
Cuts at: 1804 2162 1804
Size: 358 3175
BstAPI GCAn_nnn'nTGC
Cuts at: 910 910
Size: 3533
BstBI TT'CG_AA
Cuts at: 1894 1894
Size: 3533
BstYI r'GATC_y
Cuts at: 1 366 558 1025 1048 1383 1629 2641 2727
Size: 365 192 467 23 335 246 1012 86
Cuts at: 2727 2739 3085 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 365 346 335 246 192
86 23 12
BtgI C'CryG_G
Cuts at: 107 465 107
Size: 358 3175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1483 1664 1827 1483
Size: 181 163 3189
Fragments arranged by size:
3189 181 163
BtsI GCAGTG_nn'
Cuts at: 337 776 337
Size: 439 3094
DraI TTT'AAA
Cuts at: 532 532
Size: 3533
DrdI GACnn_nn'nnGTC
Cuts at: 1239 2097 1239
Size: 858 2675
EaeI y'GGCC_r
Cuts at: 110 167 1118 1292 1683 1710 3055 110
Size: 57 951 174 391 27 1345 588
Fragments arranged by size:
1345 951 588 391 174 57 27
EagI C'GGCC_G
Cuts at: 1118 1118
Size: 3533
EarI CTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 160 1741 2051 2197 3343 160
Size: 1581 310 146 1146 350
Fragments arranged by size:
1581 1146 350 310 146
EcoICRI GAG'CTC
Cuts at: 545 545
Size: 3533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1358 1790 2537 2805 1358
Size: 432 747 268 2086
Fragments arranged by size:
2086 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 152 392 393 672 152
Size: 240 1 279 3013
Fragments arranged by size:
3013 279 240 1
EcoRI G'AATT_C
Cuts at: 537 537
Size: 3533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1552 1552
Size: 3533
FspI TGC'GCA
Cuts at: 1314 1314
Size: 3533
HaeII r_GCGC'y
Cuts at: 967 1215 2237 3216 967
Size: 248 1022 979 1284
Fragments arranged by size:
1284 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 1375 1405 1407 1437 178
Size: 32 1165 30 2 30 2274
Fragments arranged by size:
2274 1165 32 30 30 2
HincII GTy'rAC
Cuts at: 231 572 231
Size: 341 3192
KasI G'GCGC_C
Cuts at: 963 1211 963
Size: 248 3285
KpnI G_GTAC'C
Cuts at: 553 553
Size: 3533
MluI A'CGCG_T
Cuts at: 2966 2966
Size: 3533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2388 2204
Size: 184 3349
MscI TGG'CCA
Cuts at: 1294 1294
Size: 3533
MslI CAynn'nnrTG
Cuts at: 1522 1649 1522
Size: 127 3406
MspA1I CmG'CkG
Cuts at: 244 644 958 1318 2331 2576 244
Size: 400 314 360 1013 245 1201
Fragments arranged by size:
1201 1013 400 360 314 245
NaeI GCC'GGC
Cuts at: 1714 1714
Size: 3533
NarI GG'CG_CC
Cuts at: 964 1212 964
Size: 248 3285
NcoI C'CATG_G
Cuts at: 107 107
Size: 3533
NgoMIV G'CCGG_C
Cuts at: 1712 1712
Size: 3533
NspI r_CATG'y
Cuts at: 586 1086 1993 586
Size: 500 907 2126
Fragments arranged by size:
2126 907 500
PciI A'CATG_T
Cuts at: 1989 1989
Size: 3533
PfoI T'CCnGG_A
Cuts at: 329 329
Size: 3533
PmeI GTTT'AAAC
Cuts at: 532 532
Size: 3533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2672 2704 2672
Size: 32 3501
PpuMI rG'GwC_Cy
Cuts at: 152 152
Size: 3533
PshAI GACnn'nnGTC
Cuts at: 436 436
Size: 3533
PspOMI G'GGCC_C
Cuts at: 392 392
Size: 3533
PstI C_TGCA'G
Cuts at: 347 580 1265 347
Size: 233 685 2615
Fragments arranged by size:
2615 685 233
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3533
PvuII CAG'CTG
Cuts at: 244 958 1318 244
Size: 714 360 2459
Fragments arranged by size:
2459 714 360
RsrII CG'GwC_CG
Cuts at: 1728 1728
Size: 3533
SacI G_AGCT'C
Cuts at: 547 547
Size: 3533
SalI G'TCGA_C
Cuts at: 570 570
Size: 3533
SapI GCTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
SbfI CC_TGCA'GG
Cuts at: 580 580
Size: 3533
ScaI AGT'ACT
Cuts at: 3050 3050
Size: 3533
SfcI C'TryA_G
Cuts at: 33 343 576 1261 2254 2445 33
Size: 310 233 685 993 191 1121
Fragments arranged by size:
1121 993 685 310 233 191
SfoI GGC'GCC
Cuts at: 965 1213 965
Size: 248 3285
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
SgrAI Cr'CCGG_yG
Cuts at: 385 385
Size: 3533
SmaI CCC'GGG
Cuts at: 216 555 216
Size: 339 3194
SmlI C'TyrA_G
Cuts at: 688 1603 2095 2357 688
Size: 915 492 262 1864
Fragments arranged by size:
1864 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3533
SphI G_CATG'C
Cuts at: 586 1086 586
Size: 500 3033
StyI C'CwwG_G
Cuts at: 107 667 1021 107
Size: 560 354 2619
Fragments arranged by size:
2619 560 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 241 1116 1249 1708 1917 241
Size: 875 133 459 209 1857
Fragments arranged by size:
1857 875 459 209 133
TatI w'GTAC_w
Cuts at: 3048 3048
Size: 3533
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3501
Tth111I GACn'n_nGTC
Cuts at: 1330 1330
Size: 3533
XbaI T'CTAG_A
Cuts at: 564 564
Size: 3533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1028 1028
Size: 3533
XmaI C'CCGG_G
Cuts at: 214 553 214
Size: 339 3194
XmnI GAAnn'nnTTC
Cuts at: 223 223
Size: 3533
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BpmI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI ZraI