Restriction map for
pF1KB6740
(Circular) (Six-base) MAPSORT of: pF1KB6740.seq Check: 8898 from: 1 to: 3539
pF1KB6740 3539 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 135 137 135
Size: 2 3537
AccI GT'mk_AC
Cuts at: 577 577
Size: 3539
Acc65I G'GTAC_C
Cuts at: 555 555
Size: 3539
AclI AA'CG_TT
Cuts at: 3379 3379
Size: 3539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1364 1796 2543 2811 345
Size: 1019 432 747 268 1073
Fragments arranged by size:
1073 1019 747 432 268
AflIII A'CryG_T
Cuts at: 891 1995 2972 891
Size: 1104 977 1458
Fragments arranged by size:
1458 1104 977
AgeI A'CCGG_T
Cuts at: 1927 1927
Size: 3539
AlwNI CAG_nnn'CTG
Cuts at: 844 2411 844
Size: 1567 1972
ApaI G_GGCC'C
Cuts at: 477 477
Size: 3539
ApaLI G'TGCA_C
Cuts at: 2309 2309
Size: 3539
ApoI r'AATT_y
Cuts at: 267 543 2892 3148 267
Size: 276 2349 256 658
Fragments arranged by size:
2349 658 276 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3539
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
AvaI C'yCGr_G
Cuts at: 559 559
Size: 3539
BamHI G'GATC_C
Cuts at: 210 564 210
Size: 354 3185
BanI G'GyrC_C
Cuts at: 555 969 1217 1252 555
Size: 414 248 35 2842
Fragments arranged by size:
2842 414 248 35
BanII G_rGCy'C
Cuts at: 477 553 1583 477
Size: 76 1030 2433
Fragments arranged by size:
2433 1030 76
BbeI G_GCGC'C
Cuts at: 973 1221 973
Size: 248 3291
BciVI GTATCCnnnnn_n'
Cuts at: 1437 2198 3539 1437
Size: 761 1341 1437
Fragments arranged by size:
1437 1341 761
BclI T'GATC_A
Cuts at: 1059 1059
Size: 3539
BglII A'GATC_T
Cuts at: 1054 3091 1054
Size: 2037 1502
BlpI GC'TnA_GC
Cuts at: 651 651
Size: 3539
Bme1580I G_kGCm'C
Cuts at: 477 1164 1257 2313 477
Size: 687 93 1056 1703
Fragments arranged by size:
1703 1056 687 93
BmrI ACTGGGnnnn_n'
Cuts at: 216 426 751 832 1167 3311 216
Size: 210 325 81 335 2144 444
Fragments arranged by size:
2144 444 335 325 210 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1594 2086 2384 715
Size: 879 492 298 1870
Fragments arranged by size:
1870 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3243 3243
Size: 3539
BsaAI yAC'GTr
Cuts at: 218 892 218
Size: 674 2865
BsaBI GATnn'nnATC
Cuts at: 6 1053 3090 6
Size: 1047 2037 455
Fragments arranged by size:
2037 1047 455
BsaHI Gr'CG_yC
Cuts at: 970 1218 1920 3416 970
Size: 248 702 1496 1093
Fragments arranged by size:
1496 1093 702 248
BsaWI w'CCGG_w
Cuts at: 728 1249 1927 2201 2348 2759 728
Size: 521 678 274 147 411 1508
Fragments arranged by size:
1508 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 184
Size: 3539
BseYI C'CCAG_C
Cuts at: 964 1722 2299 2795 964
Size: 758 577 496 1708
Fragments arranged by size:
1708 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 115
Size: 3539
BsiEI CG_ry'CG
Cuts at: 104 1081 1127 2335 104
Size: 977 46 1208 1308
Fragments arranged by size:
1308 1208 977 46
BsiHKAI G_wGCw'C
Cuts at: 553 1331 1521 2313 553
Size: 778 190 792 1779
Fragments arranged by size:
1779 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 451 451
Size: 3539
Bsp1286I G_dGCh'C
Cuts at: 477 553 1164 1257 1331 1521 1583 2313 477
Size: 76 611 93 74 190 62 730 1703
Fragments arranged by size:
1703 730 611 190 93 76 74 62
BspEI T'CCGG_A
Cuts at: 2759 2759
Size: 3539
BspHI T'CATG_A
Cuts at: 377 2715 377
Size: 2338 1201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 135 137 589 803 1105 1486 135
Size: 2 452 214 302 381 2188
Fragments arranged by size:
2188 452 381 302 214 2
BsrBI CCG'CTC
Cuts at: 50 1831 1885 3369 3534 50
Size: 1781 54 1484 165 55
Fragments arranged by size:
1781 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1451 1451
Size: 3539
BsrFI r'CCGG_y
Cuts at: 1537 1718 1927 1537
Size: 181 209 3149
Fragments arranged by size:
3149 209 181
BsrGI T'GTAC_A
Cuts at: 242 242
Size: 3539
BssSI C'ACGA_G
Cuts at: 1810 2168 1810
Size: 358 3181
BstAPI GCAn_nnn'nTGC
Cuts at: 916 916
Size: 3539
BstBI TT'CG_AA
Cuts at: 1900 1900
Size: 3539
BstYI r'GATC_y
Cuts at: 1 210 564 1031 1054 1389 1635 2647 2733
Size: 209 354 467 23 335 246 1012 86
Cuts at: 2733 2745 3091 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 354 346 335 246 209
86 23 12
BtgI C'CryG_G
Cuts at: 422 468 486 422
Size: 46 18 3475
Fragments arranged by size:
3475 46 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 234 1489 1670 1833 234
Size: 1255 181 163 1940
Fragments arranged by size:
1940 1255 181 163
BtsI GCAGTG_nn'
Cuts at: 782 782
Size: 3539
DraI TTT'AAA
Cuts at: 538 538
Size: 3539
DrdI GACnn_nn'nnGTC
Cuts at: 181 1245 2103 181
Size: 1064 858 1617
Fragments arranged by size:
1617 1064 858
EaeI y'GGCC_r
Cuts at: 198 1124 1298 1689 1716 3061 198
Size: 926 174 391 27 1345 676
Fragments arranged by size:
1345 926 676 391 174 27
EagI C'GGCC_G
Cuts at: 1124 1124
Size: 3539
EarI CTCTTCn'nnn_
Cuts at: 1562 1772 1562
Size: 210 3329
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1747 2057 2203 3349 1747
Size: 310 146 1146 1937
Fragments arranged by size:
1937 1146 310 146
EcoICRI GAG'CTC
Cuts at: 551 551
Size: 3539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1364 1796 2543 2811 345
Size: 1019 432 747 268 1073
Fragments arranged by size:
1073 1019 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 166 166
Size: 3539
EcoO109I rG'GnC_Cy
Cuts at: 237 473 678 237
Size: 236 205 3098
Fragments arranged by size:
3098 236 205
EcoRI G'AATT_C
Cuts at: 543 543
Size: 3539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1558 1558
Size: 3539
FspI TGC'GCA
Cuts at: 1320 1320
Size: 3539
HaeII r_GCGC'y
Cuts at: 973 1221 2243 3222 973
Size: 248 1022 979 1290
Fragments arranged by size:
1290 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1381 1411 1413 1443 1381
Size: 30 2 30 3477
Fragments arranged by size:
3477 30 30 2
HincII GTy'rAC
Cuts at: 578 578
Size: 3539
KasI G'GCGC_C
Cuts at: 969 1217 969
Size: 248 3291
KpnI G_GTAC'C
Cuts at: 559 559
Size: 3539
MluI A'CGCG_T
Cuts at: 2972 2972
Size: 3539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2394 2210
Size: 184 3355
MscI TGG'CCA
Cuts at: 1300 1300
Size: 3539
MslI CAynn'nnrTG
Cuts at: 226 1528 1655 226
Size: 1302 127 2110
Fragments arranged by size:
2110 1302 127
MspA1I CmG'CkG
Cuts at: 470 488 650 964 1324 2337 2582 470
Size: 18 162 314 360 1013 245 1427
Fragments arranged by size:
1427 1013 360 314 245 162 18
NaeI GCC'GGC
Cuts at: 1720 1720
Size: 3539
NarI GG'CG_CC
Cuts at: 970 1218 970
Size: 248 3291
NcoI C'CATG_G
Cuts at: 422 422
Size: 3539
NgoMIV G'CCGG_C
Cuts at: 1718 1718
Size: 3539
NspI r_CATG'y
Cuts at: 120 592 1092 1999 120
Size: 472 500 907 1660
Fragments arranged by size:
1660 907 500 472
PciI A'CATG_T
Cuts at: 1995 1995
Size: 3539
PmeI GTTT'AAAC
Cuts at: 538 538
Size: 3539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 2710 2678
Size: 32 3507
PpuMI rG'GwC_Cy
Cuts at: 237 237
Size: 3539
PspOMI G'GGCC_C
Cuts at: 473 473
Size: 3539
PstI C_TGCA'G
Cuts at: 586 1271 586
Size: 685 2854
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3539
PvuII CAG'CTG
Cuts at: 964 1324 964
Size: 360 3179
RsrII CG'GwC_CG
Cuts at: 1734 1734
Size: 3539
SacI G_AGCT'C
Cuts at: 553 553
Size: 3539
SacII CC_GC'GG
Cuts at: 471 489 471
Size: 18 3521
SalI G'TCGA_C
Cuts at: 576 576
Size: 3539
SapI GCTCTTCn'nnn_
Cuts at: 1562 1772 1562
Size: 210 3329
SbfI CC_TGCA'GG
Cuts at: 586 586
Size: 3539
ScaI AGT'ACT
Cuts at: 3056 3056
Size: 3539
SfcI C'TryA_G
Cuts at: 33 582 1267 2260 2451 33
Size: 549 685 993 191 1121
Fragments arranged by size:
1121 993 685 549 191
SfoI GGC'GCC
Cuts at: 971 1219 971
Size: 248 3291
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
SmaI CCC'GGG
Cuts at: 561 561
Size: 3539
SmlI C'TyrA_G
Cuts at: 694 1609 2101 2363 694
Size: 915 492 262 1870
Fragments arranged by size:
1870 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3539
SphI G_CATG'C
Cuts at: 592 1092 592
Size: 500 3039
StyI C'CwwG_G
Cuts at: 422 506 673 1027 422
Size: 84 167 354 2934
Fragments arranged by size:
2934 354 167 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1122 1255 1714 1923 1122
Size: 133 459 209 2738
Fragments arranged by size:
2738 459 209 133
TatI w'GTAC_w
Cuts at: 242 343 3054 242
Size: 101 2711 727
Fragments arranged by size:
2711 727 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 202 234 3056 3088 202
Size: 32 2822 32 653
Fragments arranged by size:
2822 653 32 32
Tth111I GACn'n_nGTC
Cuts at: 235 1336 235
Size: 1101 2438
XbaI T'CTAG_A
Cuts at: 570 570
Size: 3539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1034 1034
Size: 3539
XmaI C'CCGG_G
Cuts at: 559 559
Size: 3539
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI