Restriction map for
pF1KB6745
(Circular) (Six-base) MAPSORT of: pF1KB6745.seq Check: 3855 from: 1 to: 3416
pF1KB6745 3416 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 454 454
Size: 3416
Acc65I G'GTAC_C
Cuts at: 432 432
Size: 3416
AclI AA'CG_TT
Cuts at: 3256 3256
Size: 3416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 227 355 1241 1673 2420 2688 221
Size: 6 128 886 432 747 268 949
Fragments arranged by size:
949 886 747 432 268 128 6
AfeI AGC'GCT
Cuts at: 198 198
Size: 3416
AflIII A'CryG_T
Cuts at: 768 1872 2849 768
Size: 1104 977 1335
Fragments arranged by size:
1335 1104 977
AgeI A'CCGG_T
Cuts at: 1804 1804
Size: 3416
AlwNI CAG_nnn'CTG
Cuts at: 231 721 2288 231
Size: 490 1567 1359
Fragments arranged by size:
1567 1359 490
ApaLI G'TGCA_C
Cuts at: 2186 2186
Size: 3416
ApoI r'AATT_y
Cuts at: 350 420 2769 3025 350
Size: 70 2349 256 741
Fragments arranged by size:
2349 741 256 70
AseI AT'TA_AT
Cuts at: 20 20
Size: 3416
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3416
AvaI C'yCGr_G
Cuts at: 142 436 142
Size: 294 3122
BamHI G'GATC_C
Cuts at: 441 441
Size: 3416
BanI G'GyrC_C
Cuts at: 432 846 1094 1129 432
Size: 414 248 35 2719
Fragments arranged by size:
2719 414 248 35
BanII G_rGCy'C
Cuts at: 115 430 1460 115
Size: 315 1030 2071
Fragments arranged by size:
2071 1030 315
BbeI G_GCGC'C
Cuts at: 850 1098 850
Size: 248 3168
BbsI GAAGACnn'nnnn_
Cuts at: 247 247
Size: 3416
BciVI GTATCCnnnnn_n'
Cuts at: 1314 2075 3416 1314
Size: 761 1341 1314
Fragments arranged by size:
1341 1314 761
BclI T'GATC_A
Cuts at: 287 936 287
Size: 649 2767
BglII A'GATC_T
Cuts at: 931 2968 931
Size: 2037 1379
BlpI GC'TnA_GC
Cuts at: 528 528
Size: 3416
Bme1580I G_kGCm'C
Cuts at: 1041 1134 2190 1041
Size: 93 1056 2267
Fragments arranged by size:
2267 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 628 709 1044 3188 628
Size: 81 335 2144 856
Fragments arranged by size:
2144 856 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 3416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1471 1963 2261 592
Size: 879 492 298 1747
Fragments arranged by size:
1747 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3120 3120
Size: 3416
BsaAI yAC'GTr
Cuts at: 769 769
Size: 3416
BsaBI GATnn'nnATC
Cuts at: 6 930 2967 6
Size: 924 2037 455
Fragments arranged by size:
2037 924 455
BsaHI Gr'CG_yC
Cuts at: 847 1095 1797 3293 847
Size: 248 702 1496 970
Fragments arranged by size:
1496 970 702 248
BsaWI w'CCGG_w
Cuts at: 605 1126 1804 2078 2225 2636 605
Size: 521 678 274 147 411 1385
Fragments arranged by size:
1385 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 841 1599 2176 2672 841
Size: 758 577 496 1585
Fragments arranged by size:
1585 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 958 1004 2212 104
Size: 854 46 1208 1308
Fragments arranged by size:
1308 1208 854 46
BsiHKAI G_wGCw'C
Cuts at: 115 430 1208 1398 2190 115
Size: 315 778 190 792 1341
Fragments arranged by size:
1341 792 778 315 190
Bsp1286I G_dGCh'C
Cuts at: 115 430 1041 1134 1208 1398 1460 2190 115
Size: 315 611 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 315 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2636 2636
Size: 3416
BspHI T'CATG_A
Cuts at: 2592 2592
Size: 3416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 371 466 680 982 1363 230
Size: 141 95 214 302 381 2283
Fragments arranged by size:
2283 381 302 214 141 95
BsrBI CCG'CTC
Cuts at: 50 1708 1762 3246 3411 50
Size: 1658 54 1484 165 55
Fragments arranged by size:
1658 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1328 1328
Size: 3416
BsrFI r'CCGG_y
Cuts at: 1414 1595 1804 1414
Size: 181 209 3026
Fragments arranged by size:
3026 209 181
BsrGI T'GTAC_A
Cuts at: 377 377
Size: 3416
BssSI C'ACGA_G
Cuts at: 1687 2045 1687
Size: 358 3058
BstAPI GCAn_nnn'nTGC
Cuts at: 793 793
Size: 3416
BstBI TT'CG_AA
Cuts at: 1777 1777
Size: 3416
BstYI r'GATC_y
Cuts at: 1 441 908 931 1266 1512 2524 2610 2622
Size: 440 467 23 335 246 1012 86 12
Cuts at: 2622 2968 1
Size: 346 449
Fragments arranged by size:
1012 467 449 440 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1366 1547 1710 1366
Size: 181 163 3072
Fragments arranged by size:
3072 181 163
BtsI GCAGTG_nn'
Cuts at: 232 303 311 659 232
Size: 71 8 348 2989
Fragments arranged by size:
2989 348 71 8
DraI TTT'AAA
Cuts at: 415 415
Size: 3416
DrdI GACnn_nn'nnGTC
Cuts at: 1122 1980 1122
Size: 858 2558
EaeI y'GGCC_r
Cuts at: 1001 1175 1566 1593 2938 1001
Size: 174 391 27 1345 1479
Fragments arranged by size:
1479 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1001 1001
Size: 3416
EarI CTCTTCn'nnn_
Cuts at: 1439 1649 1439
Size: 210 3206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1624 1934 2080 3226 1624
Size: 310 146 1146 1814
Fragments arranged by size:
1814 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 428 113
Size: 315 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 227 301 355 1241 1673 2420 2688 221
Size: 6 74 54 886 432 747 268 949
Fragments arranged by size:
949 886 747 432 268 74 54 6
EcoO109I rG'GnC_Cy
Cuts at: 219 555 219
Size: 336 3080
EcoRI G'AATT_C
Cuts at: 420 420
Size: 3416
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1435 1435
Size: 3416
FspI TGC'GCA
Cuts at: 1197 1197
Size: 3416
HaeII r_GCGC'y
Cuts at: 200 850 1098 2120 3099 200
Size: 650 248 1022 979 517
Fragments arranged by size:
1022 979 650 517 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1258 1288 1290 1320 1258
Size: 30 2 30 3354
Fragments arranged by size:
3354 30 30 2
HincII GTy'rAC
Cuts at: 455 455
Size: 3416
KasI G'GCGC_C
Cuts at: 846 1094 846
Size: 248 3168
KpnI G_GTAC'C
Cuts at: 436 436
Size: 3416
MluI A'CGCG_T
Cuts at: 2849 2849
Size: 3416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2087 2271 2087
Size: 184 3232
MscI TGG'CCA
Cuts at: 1177 1177
Size: 3416
MslI CAynn'nnrTG
Cuts at: 1405 1532 1405
Size: 127 3289
MspA1I CmG'CkG
Cuts at: 375 527 841 1201 2214 2459 375
Size: 152 314 360 1013 245 1332
Fragments arranged by size:
1332 1013 360 314 245 152
NaeI GCC'GGC
Cuts at: 1597 1597
Size: 3416
NarI GG'CG_CC
Cuts at: 847 1095 847
Size: 248 3168
NgoMIV G'CCGG_C
Cuts at: 1595 1595
Size: 3416
NspI r_CATG'y
Cuts at: 469 969 1876 469
Size: 500 907 2009
Fragments arranged by size:
2009 907 500
PciI A'CATG_T
Cuts at: 1872 1872
Size: 3416
PmeI GTTT'AAAC
Cuts at: 415 415
Size: 3416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 3384
PpuMI rG'GwC_Cy
Cuts at: 219 219
Size: 3416
PstI C_TGCA'G
Cuts at: 227 463 1148 227
Size: 236 685 2495
Fragments arranged by size:
2495 685 236
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3416
PvuII CAG'CTG
Cuts at: 841 1201 841
Size: 360 3056
RsrII CG'GwC_CG
Cuts at: 1611 1611
Size: 3416
SacI G_AGCT'C
Cuts at: 115 430 115
Size: 315 3101
SalI G'TCGA_C
Cuts at: 453 453
Size: 3416
SapI GCTCTTCn'nnn_
Cuts at: 1439 1649 1439
Size: 210 3206
SbfI CC_TGCA'GG
Cuts at: 463 463
Size: 3416
ScaI AGT'ACT
Cuts at: 2933 2933
Size: 3416
SfcI C'TryA_G
Cuts at: 33 223 459 1144 2137 2328 33
Size: 190 236 685 993 191 1121
Fragments arranged by size:
1121 993 685 236 191 190
SfoI GGC'GCC
Cuts at: 848 1096 848
Size: 248 3168
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3416
SmaI CCC'GGG
Cuts at: 144 438 144
Size: 294 3122
SmlI C'TyrA_G
Cuts at: 571 1486 1978 2240 571
Size: 915 492 262 1747
Fragments arranged by size:
1747 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3416
SphI G_CATG'C
Cuts at: 469 969 469
Size: 500 2916
StyI C'CwwG_G
Cuts at: 550 904 550
Size: 354 3062
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 999 1132 1591 1800 999
Size: 133 459 209 2615
Fragments arranged by size:
2615 459 209 133
TatI w'GTAC_w
Cuts at: 377 2931 377
Size: 2554 862
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 211 243 2933 2965 211
Size: 32 2690 32 662
Fragments arranged by size:
2690 662 32 32
Tth111I GACn'n_nGTC
Cuts at: 1213 1213
Size: 3416
XbaI T'CTAG_A
Cuts at: 447 447
Size: 3416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 911 911
Size: 3416
XmaI C'CCGG_G
Cuts at: 142 436 142
Size: 294 3122
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI