Restriction map for
pF1KB6754
(Circular) (Six-base) MAPSORT of: pF1KB6754.seq Check: 5453 from: 1 to: 3551
pF1KB6754 3551 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 589 589
Size: 3551
Acc65I G'GTAC_C
Cuts at: 567 567
Size: 3551
AclI AA'CG_TT
Cuts at: 3391 3391
Size: 3551
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 492 514 1376 1808 2555 2823 307
Size: 185 22 862 432 747 268 1035
Fragments arranged by size:
1035 862 747 432 268 185 22
AflIII A'CryG_T
Cuts at: 903 2007 2984 903
Size: 1104 977 1470
Fragments arranged by size:
1470 1104 977
AgeI A'CCGG_T
Cuts at: 1939 1939
Size: 3551
AlwNI CAG_nnn'CTG
Cuts at: 492 856 2423 492
Size: 364 1567 1620
Fragments arranged by size:
1620 1567 364
ApaLI G'TGCA_C
Cuts at: 2321 2321
Size: 3551
ApoI r'AATT_y
Cuts at: 555 2904 3160 555
Size: 2349 256 946
Fragments arranged by size:
2349 946 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3551
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3551
AvaI C'yCGr_G
Cuts at: 571 571
Size: 3551
BamHI G'GATC_C
Cuts at: 450 576 450
Size: 126 3425
BanI G'GyrC_C
Cuts at: 567 981 1229 1264 567
Size: 414 248 35 2854
Fragments arranged by size:
2854 414 248 35
BanII G_rGCy'C
Cuts at: 565 1595 565
Size: 1030 2521
BbeI G_GCGC'C
Cuts at: 985 1233 985
Size: 248 3303
BbsI GAAGACnn'nnnn_
Cuts at: 498 498
Size: 3551
BciVI GTATCCnnnnn_n'
Cuts at: 1449 2210 3551 1449
Size: 761 1341 1449
Fragments arranged by size:
1449 1341 761
BclI T'GATC_A
Cuts at: 242 1071 242
Size: 829 2722
BfrBI ATG'CAT
Cuts at: 331 331
Size: 3551
BglII A'GATC_T
Cuts at: 1066 3103 1066
Size: 2037 1514
BlpI GC'TnA_GC
Cuts at: 663 663
Size: 3551
Bme1580I G_kGCm'C
Cuts at: 146 1176 1269 2325 146
Size: 1030 93 1056 1372
Fragments arranged by size:
1372 1056 1030 93
BmrI ACTGGGnnnn_n'
Cuts at: 763 844 1179 3323 763
Size: 81 335 2144 991
Fragments arranged by size:
2144 991 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1918 1918
Size: 3551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 193
Size: 3551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 1606 2098 2396 727
Size: 879 492 298 1882
Fragments arranged by size:
1882 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3255 3255
Size: 3551
BsaAI yAC'GTr
Cuts at: 138 904 138
Size: 766 2785
BsaBI GATnn'nnATC
Cuts at: 6 437 1065 3102 6
Size: 431 628 2037 455
Fragments arranged by size:
2037 628 455 431
BsaHI Gr'CG_yC
Cuts at: 982 1230 1932 3428 982
Size: 248 702 1496 1105
Fragments arranged by size:
1496 1105 702 248
BsaWI w'CCGG_w
Cuts at: 189 740 1261 1939 2213 2360 2771 189
Size: 551 521 678 274 147 411 969
Fragments arranged by size:
969 678 551 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 166 196 166
Size: 30 3521
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 332 332
Size: 3551
BseYI C'CCAG_C
Cuts at: 976 1734 2311 2807 976
Size: 758 577 496 1720
Fragments arranged by size:
1720 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 190 1093 1139 2347 104
Size: 86 903 46 1208 1308
Fragments arranged by size:
1308 1208 903 86 46
BsiHKAI G_wGCw'C
Cuts at: 565 1343 1533 2325 565
Size: 778 190 792 1791
Fragments arranged by size:
1791 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 146 565 1176 1269 1343 1533 1595 2325 146
Size: 419 611 93 74 190 62 730 1372
Fragments arranged by size:
1372 730 611 419 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2771 2771
Size: 3551
BspHI T'CATG_A
Cuts at: 239 2727 239
Size: 2488 1063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 601 815 1117 1498 601
Size: 214 302 381 2654
Fragments arranged by size:
2654 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1843 1897 3381 3546 50
Size: 1793 54 1484 165 55
Fragments arranged by size:
1793 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1463 1463
Size: 3551
BsrFI r'CCGG_y
Cuts at: 359 1549 1730 1939 359
Size: 1190 181 209 1971
Fragments arranged by size:
1971 1190 209 181
BsrGI T'GTAC_A
Cuts at: 261 261
Size: 3551
BssSI C'ACGA_G
Cuts at: 163 1822 2180 163
Size: 1659 358 1534
Fragments arranged by size:
1659 1534 358
BstAPI GCAn_nnn'nTGC
Cuts at: 928 928
Size: 3551
BstBI TT'CG_AA
Cuts at: 1912 1912
Size: 3551
BstYI r'GATC_y
Cuts at: 1 450 576 1043 1066 1401 1647 2659 2745
Size: 449 126 467 23 335 246 1012 86
Cuts at: 2745 2757 3103 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 449 346 335 246 126
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3551
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1501 1682 1845 1501
Size: 181 163 3207
Fragments arranged by size:
3207 181 163
BtsI GCAGTG_nn'
Cuts at: 794 794
Size: 3551
DraI TTT'AAA
Cuts at: 550 550
Size: 3551
DrdI GACnn_nn'nnGTC
Cuts at: 1257 2115 1257
Size: 858 2693
EaeI y'GGCC_r
Cuts at: 159 1136 1310 1701 1728 3073 159
Size: 977 174 391 27 1345 637
Fragments arranged by size:
1345 977 637 391 174 27
EagI C'GGCC_G
Cuts at: 1136 1136
Size: 3551
EarI CTCTTCn'nnn_
Cuts at: 283 1574 1784 283
Size: 1291 210 2050
Fragments arranged by size:
2050 1291 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1759 2069 2215 3361 1759
Size: 310 146 1146 1949
Fragments arranged by size:
1949 1146 310 146
EcoICRI GAG'CTC
Cuts at: 563 563
Size: 3551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 307 492 514 1376 1808 2555 2823 193
Size: 114 185 22 862 432 747 268 921
Fragments arranged by size:
921 862 747 432 268 185 114 22
EcoO109I rG'GnC_Cy
Cuts at: 489 690 489
Size: 201 3350
EcoRI G'AATT_C
Cuts at: 555 555
Size: 3551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1570 1570
Size: 3551
FspI TGC'GCA
Cuts at: 1332 1332
Size: 3551
HaeII r_GCGC'y
Cuts at: 985 1233 2255 3234 985
Size: 248 1022 979 1302
Fragments arranged by size:
1302 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1393 1423 1425 1455 1393
Size: 30 2 30 3489
Fragments arranged by size:
3489 30 30 2
HincII GTy'rAC
Cuts at: 590 590
Size: 3551
KasI G'GCGC_C
Cuts at: 981 1229 981
Size: 248 3303
KpnI G_GTAC'C
Cuts at: 571 571
Size: 3551
MluI A'CGCG_T
Cuts at: 2984 2984
Size: 3551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2222 2406 2222
Size: 184 3367
MscI TGG'CCA
Cuts at: 1312 1312
Size: 3551
MslI CAynn'nnrTG
Cuts at: 1540 1667 1540
Size: 127 3424
MspA1I CmG'CkG
Cuts at: 662 976 1336 2349 2594 662
Size: 314 360 1013 245 1619
Fragments arranged by size:
1619 1013 360 314 245
NaeI GCC'GGC
Cuts at: 361 1732 361
Size: 1371 2180
NarI GG'CG_CC
Cuts at: 982 1230 982
Size: 248 3303
NcoI C'CATG_G
Cuts at: 107 107
Size: 3551
NgoMIV G'CCGG_C
Cuts at: 359 1730 359
Size: 1371 2180
NsiI A_TGCA'T
Cuts at: 333 333
Size: 3551
NspI r_CATG'y
Cuts at: 604 1104 2011 604
Size: 500 907 2144
Fragments arranged by size:
2144 907 500
PciI A'CATG_T
Cuts at: 2007 2007
Size: 3551
PfoI T'CCnGG_A
Cuts at: 170 485 170
Size: 315 3236
PmeI GTTT'AAAC
Cuts at: 550 550
Size: 3551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2690 2722 2690
Size: 32 3519
PpuMI rG'GwC_Cy
Cuts at: 489 489
Size: 3551
PstI C_TGCA'G
Cuts at: 598 1283 598
Size: 685 2866
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3551
PvuII CAG'CTG
Cuts at: 976 1336 976
Size: 360 3191
RsrII CG'GwC_CG
Cuts at: 1746 1746
Size: 3551
SacI G_AGCT'C
Cuts at: 565 565
Size: 3551
SalI G'TCGA_C
Cuts at: 588 588
Size: 3551
SapI GCTCTTCn'nnn_
Cuts at: 1574 1784 1574
Size: 210 3341
SbfI CC_TGCA'GG
Cuts at: 598 598
Size: 3551
ScaI AGT'ACT
Cuts at: 3068 3068
Size: 3551
SfcI C'TryA_G
Cuts at: 33 594 1279 2272 2463 33
Size: 561 685 993 191 1121
Fragments arranged by size:
1121 993 685 561 191
SfoI GGC'GCC
Cuts at: 983 1231 983
Size: 248 3303
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3551
SmaI CCC'GGG
Cuts at: 573 573
Size: 3551
SmlI C'TyrA_G
Cuts at: 706 1621 2113 2375 706
Size: 915 492 262 1882
Fragments arranged by size:
1882 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3551
SphI G_CATG'C
Cuts at: 604 1104 604
Size: 500 3051
StyI C'CwwG_G
Cuts at: 107 446 685 1039 107
Size: 339 239 354 2619
Fragments arranged by size:
2619 354 339 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 365 1134 1267 1726 1935 365
Size: 769 133 459 209 1981
Fragments arranged by size:
1981 769 459 209 133
TatI w'GTAC_w
Cuts at: 261 3066 261
Size: 2805 746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 481 513 3068 3100 481
Size: 32 2555 32 932
Fragments arranged by size:
2555 932 32 32
Tth111I GACn'n_nGTC
Cuts at: 1348 1348
Size: 3551
XbaI T'CTAG_A
Cuts at: 582 582
Size: 3551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1046 1046
Size: 3551
XmaI C'CCGG_G
Cuts at: 571 571
Size: 3551
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI