Restriction map for
pF1KB6755
(Circular) (Six-base) MAPSORT of: pF1KB6755.seq Check: 1556 from: 1 to: 3428
pF1KB6755 3428 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 466 466
Size: 3428
Acc65I G'GTAC_C
Cuts at: 444 444
Size: 3428
AclI AA'CG_TT
Cuts at: 3268 3268
Size: 3428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1253 1685 2432 2700 247
Size: 1006 432 747 268 975
Fragments arranged by size:
1006 975 747 432 268
AflIII A'CryG_T
Cuts at: 780 1884 2861 780
Size: 1104 977 1347
Fragments arranged by size:
1347 1104 977
AgeI A'CCGG_T
Cuts at: 1816 1816
Size: 3428
AlwNI CAG_nnn'CTG
Cuts at: 733 2300 733
Size: 1567 1861
ApaLI G'TGCA_C
Cuts at: 2198 2198
Size: 3428
ApoI r'AATT_y
Cuts at: 432 2781 3037 432
Size: 2349 256 823
Fragments arranged by size:
2349 823 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3428
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3428
AvaI C'yCGr_G
Cuts at: 162 214 448 162
Size: 52 234 3142
Fragments arranged by size:
3142 234 52
BamHI G'GATC_C
Cuts at: 453 453
Size: 3428
BanI G'GyrC_C
Cuts at: 444 858 1106 1141 444
Size: 414 248 35 2731
Fragments arranged by size:
2731 414 248 35
BanII G_rGCy'C
Cuts at: 442 1472 442
Size: 1030 2398
BbeI G_GCGC'C
Cuts at: 862 1110 862
Size: 248 3180
BbvCI CC'TCA_GC
Cuts at: 233 233
Size: 3428
BciVI GTATCCnnnnn_n'
Cuts at: 1326 2087 3428 1326
Size: 761 1341 1326
Fragments arranged by size:
1341 1326 761
BclI T'GATC_A
Cuts at: 948 948
Size: 3428
BglII A'GATC_T
Cuts at: 943 2980 943
Size: 2037 1391
BlpI GC'TnA_GC
Cuts at: 540 540
Size: 3428
Bme1580I G_kGCm'C
Cuts at: 1053 1146 2202 1053
Size: 93 1056 2279
Fragments arranged by size:
2279 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 291 395 640 721 1056 3200 291
Size: 104 245 81 335 2144 519
Fragments arranged by size:
2144 519 335 245 104 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1795 1795
Size: 3428
Bpu10I CC'TnA_GC
Cuts at: 233 233
Size: 3428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1483 1975 2273 604
Size: 879 492 298 1759
Fragments arranged by size:
1759 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 3132 217
Size: 2915 513
BsaAI yAC'GTr
Cuts at: 781 781
Size: 3428
BsaBI GATnn'nnATC
Cuts at: 6 942 2979 6
Size: 936 2037 455
Fragments arranged by size:
2037 936 455
BsaHI Gr'CG_yC
Cuts at: 120 859 1107 1809 3305 120
Size: 739 248 702 1496 243
Fragments arranged by size:
1496 739 702 248 243
BsaWI w'CCGG_w
Cuts at: 362 617 1138 1816 2090 2237 2648 362
Size: 255 521 678 274 147 411 1142
Fragments arranged by size:
1142 678 521 411 274 255 147
BseYI C'CCAG_C
Cuts at: 853 1611 2188 2684 853
Size: 758 577 496 1597
Fragments arranged by size:
1597 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 970 1016 2224 104
Size: 866 46 1208 1308
Fragments arranged by size:
1308 1208 866 46
BsiHKAI G_wGCw'C
Cuts at: 442 1220 1410 2202 442
Size: 778 190 792 1668
Fragments arranged by size:
1668 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 442 1053 1146 1220 1410 1472 2202 442
Size: 611 93 74 190 62 730 1668
Fragments arranged by size:
1668 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 362 2648 362
Size: 2286 1142
BspHI T'CATG_A
Cuts at: 2604 2604
Size: 3428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 147 478 692 994 1375 147
Size: 331 214 302 381 2200
Fragments arranged by size:
2200 381 331 302 214
BsrBI CCG'CTC
Cuts at: 50 1720 1774 3258 3423 50
Size: 1670 54 1484 165 55
Fragments arranged by size:
1670 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1340 1340
Size: 3428
BsrFI r'CCGG_y
Cuts at: 1426 1607 1816 1426
Size: 181 209 3038
Fragments arranged by size:
3038 209 181
BssSI C'ACGA_G
Cuts at: 1699 2057 1699
Size: 358 3070
BstAPI GCAn_nnn'nTGC
Cuts at: 805 805
Size: 3428
BstBI TT'CG_AA
Cuts at: 1789 1789
Size: 3428
BstYI r'GATC_y
Cuts at: 1 453 920 943 1278 1524 2536 2622 2634
Size: 452 467 23 335 246 1012 86 12
Cuts at: 2634 2980 1
Size: 346 449
Fragments arranged by size:
1012 467 452 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1378 1559 1722 1378
Size: 181 163 3084
Fragments arranged by size:
3084 181 163
BtsI GCAGTG_nn'
Cuts at: 671 671
Size: 3428
DraI TTT'AAA
Cuts at: 384 427 384
Size: 43 3385
DrdI GACnn_nn'nnGTC
Cuts at: 1134 1992 1134
Size: 858 2570
EaeI y'GGCC_r
Cuts at: 1013 1187 1578 1605 2950 1013
Size: 174 391 27 1345 1491
Fragments arranged by size:
1491 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1013 1013
Size: 3428
EarI CTCTTCn'nnn_
Cuts at: 1451 1661 1451
Size: 210 3218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1636 1946 2092 3238 1636
Size: 310 146 1146 1826
Fragments arranged by size:
1826 1146 310 146
EcoICRI GAG'CTC
Cuts at: 440 440
Size: 3428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1253 1685 2432 2700 247
Size: 1006 432 747 268 975
Fragments arranged by size:
1006 975 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 159 567 159
Size: 408 3020
EcoRI G'AATT_C
Cuts at: 432 432
Size: 3428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1447 1447
Size: 3428
FspI TGC'GCA
Cuts at: 1209 1209
Size: 3428
HaeII r_GCGC'y
Cuts at: 233 862 1110 2132 3111 233
Size: 629 248 1022 979 550
Fragments arranged by size:
1022 979 629 550 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1270 1300 1302 1332 1270
Size: 30 2 30 3366
Fragments arranged by size:
3366 30 30 2
HincII GTy'rAC
Cuts at: 467 467
Size: 3428
KasI G'GCGC_C
Cuts at: 858 1106 858
Size: 248 3180
KpnI G_GTAC'C
Cuts at: 448 448
Size: 3428
MluI A'CGCG_T
Cuts at: 2861 2861
Size: 3428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2099 2283 2099
Size: 184 3244
MscI TGG'CCA
Cuts at: 1189 1189
Size: 3428
MslI CAynn'nnrTG
Cuts at: 1417 1544 1417
Size: 127 3301
MspA1I CmG'CkG
Cuts at: 539 853 1213 2226 2471 539
Size: 314 360 1013 245 1496
Fragments arranged by size:
1496 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1609 1609
Size: 3428
NarI GG'CG_CC
Cuts at: 859 1107 859
Size: 248 3180
NgoMIV G'CCGG_C
Cuts at: 1607 1607
Size: 3428
NspI r_CATG'y
Cuts at: 481 981 1888 481
Size: 500 907 2021
Fragments arranged by size:
2021 907 500
PciI A'CATG_T
Cuts at: 1884 1884
Size: 3428
PmeI GTTT'AAAC
Cuts at: 427 427
Size: 3428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2567 2599 2567
Size: 32 3396
PpuMI rG'GwC_Cy
Cuts at: 159 159
Size: 3428
PstI C_TGCA'G
Cuts at: 243 475 1160 243
Size: 232 685 2511
Fragments arranged by size:
2511 685 232
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3428
PvuII CAG'CTG
Cuts at: 853 1213 853
Size: 360 3068
RsrII CG'GwC_CG
Cuts at: 1623 1623
Size: 3428
SacI G_AGCT'C
Cuts at: 442 442
Size: 3428
SalI G'TCGA_C
Cuts at: 465 465
Size: 3428
SapI GCTCTTCn'nnn_
Cuts at: 1451 1661 1451
Size: 210 3218
SbfI CC_TGCA'GG
Cuts at: 475 475
Size: 3428
ScaI AGT'ACT
Cuts at: 2945 2945
Size: 3428
SfcI C'TryA_G
Cuts at: 33 224 239 471 1156 2149 2340 33
Size: 191 15 232 685 993 191 1121
Fragments arranged by size:
1121 993 685 232 191 191 15
SfoI GGC'GCC
Cuts at: 860 1108 860
Size: 248 3180
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3428
SmaI CCC'GGG
Cuts at: 450 450
Size: 3428
SmlI C'TyrA_G
Cuts at: 583 1498 1990 2252 583
Size: 915 492 262 1759
Fragments arranged by size:
1759 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3428
SphI G_CATG'C
Cuts at: 481 981 481
Size: 500 2928
StyI C'CwwG_G
Cuts at: 207 562 916 207
Size: 355 354 2719
Fragments arranged by size:
2719 355 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1011 1144 1603 1812 1011
Size: 133 459 209 2627
Fragments arranged by size:
2627 459 209 133
TatI w'GTAC_w
Cuts at: 2943 2943
Size: 3428
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 3396
Tth111I GACn'n_nGTC
Cuts at: 1225 1225
Size: 3428
XbaI T'CTAG_A
Cuts at: 459 459
Size: 3428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 923 923
Size: 3428
XmaI C'CCGG_G
Cuts at: 448 448
Size: 3428
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI