Restriction map for
pF1KB6757
(Circular) (Six-base) MAPSORT of: pF1KB6757.seq Check: 5825 from: 1 to: 3431
pF1KB6757 3431 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 469 469
Size: 3431
Acc65I G'GTAC_C
Cuts at: 447 447
Size: 3431
AclI AA'CG_TT
Cuts at: 3271 3271
Size: 3431
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1256 1688 2435 2703 1256
Size: 432 747 268 1984
Fragments arranged by size:
1984 747 432 268
AflII C'TTAA_G
Cuts at: 409 409
Size: 3431
AflIII A'CryG_T
Cuts at: 783 1887 2864 783
Size: 1104 977 1350
Fragments arranged by size:
1350 1104 977
AgeI A'CCGG_T
Cuts at: 1819 1819
Size: 3431
AlwNI CAG_nnn'CTG
Cuts at: 736 2303 736
Size: 1567 1864
ApaLI G'TGCA_C
Cuts at: 2201 2201
Size: 3431
ApoI r'AATT_y
Cuts at: 435 2784 3040 435
Size: 2349 256 826
Fragments arranged by size:
2349 826 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3431
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3431
AvaI C'yCGr_G
Cuts at: 451 451
Size: 3431
BamHI G'GATC_C
Cuts at: 252 456 252
Size: 204 3227
BanI G'GyrC_C
Cuts at: 447 861 1109 1144 447
Size: 414 248 35 2734
Fragments arranged by size:
2734 414 248 35
BanII G_rGCy'C
Cuts at: 445 1475 445
Size: 1030 2401
BbeI G_GCGC'C
Cuts at: 865 1113 865
Size: 248 3183
BbsI GAAGACnn'nnnn_
Cuts at: 196 196
Size: 3431
BciVI GTATCCnnnnn_n'
Cuts at: 1329 2090 3431 1329
Size: 761 1341 1329
Fragments arranged by size:
1341 1329 761
BclI T'GATC_A
Cuts at: 951 951
Size: 3431
BglII A'GATC_T
Cuts at: 946 2983 946
Size: 2037 1394
BlpI GC'TnA_GC
Cuts at: 543 543
Size: 3431
Bme1580I G_kGCm'C
Cuts at: 1056 1149 2205 1056
Size: 93 1056 2282
Fragments arranged by size:
2282 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 643 724 1059 3203 643
Size: 81 335 2144 871
Fragments arranged by size:
2144 871 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1798 1798
Size: 3431
Bpu10I CC'TnA_GC
Cuts at: 257 257
Size: 3431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1486 1978 2276 607
Size: 879 492 298 1762
Fragments arranged by size:
1762 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3135 3135
Size: 3431
BsaAI yAC'GTr
Cuts at: 784 784
Size: 3431
BsaBI GATnn'nnATC
Cuts at: 6 945 2982 6
Size: 939 2037 455
Fragments arranged by size:
2037 939 455
BsaHI Gr'CG_yC
Cuts at: 862 1110 1812 3308 862
Size: 248 702 1496 985
Fragments arranged by size:
1496 985 702 248
BsaWI w'CCGG_w
Cuts at: 620 1141 1819 2093 2240 2651 620
Size: 521 678 274 147 411 1400
Fragments arranged by size:
1400 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 373
Size: 3431
BseYI C'CCAG_C
Cuts at: 856 1614 2191 2687 856
Size: 758 577 496 1600
Fragments arranged by size:
1600 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 973 1019 2227 104
Size: 869 46 1208 1308
Fragments arranged by size:
1308 1208 869 46
BsiHKAI G_wGCw'C
Cuts at: 445 1223 1413 2205 445
Size: 778 190 792 1671
Fragments arranged by size:
1671 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 445 1056 1149 1223 1413 1475 2205 445
Size: 611 93 74 190 62 730 1671
Fragments arranged by size:
1671 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2651 2651
Size: 3431
BspHI T'CATG_A
Cuts at: 2607 2607
Size: 3431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 481 695 997 1378 481
Size: 214 302 381 2534
Fragments arranged by size:
2534 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1723 1777 3261 3426 50
Size: 1673 54 1484 165 55
Fragments arranged by size:
1673 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1343 1343
Size: 3431
BsrFI r'CCGG_y
Cuts at: 1429 1610 1819 1429
Size: 181 209 3041
Fragments arranged by size:
3041 209 181
BssSI C'ACGA_G
Cuts at: 1702 2060 1702
Size: 358 3073
BstAPI GCAn_nnn'nTGC
Cuts at: 808 808
Size: 3431
BstBI TT'CG_AA
Cuts at: 1792 1792
Size: 3431
BstYI r'GATC_y
Cuts at: 1 252 372 456 923 946 1281 1527 2539
Size: 251 120 84 467 23 335 246 1012
Cuts at: 2539 2625 2637 2983 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 251 246 120
86 84 23 12
BtgI C'CryG_G
Cuts at: 119 119
Size: 3431
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1381 1562 1725 1381
Size: 181 163 3087
Fragments arranged by size:
3087 181 163
BtsI GCAGTG_nn'
Cuts at: 674 674
Size: 3431
DraI TTT'AAA
Cuts at: 430 430
Size: 3431
DrdI GACnn_nn'nnGTC
Cuts at: 1137 1995 1137
Size: 858 2573
EaeI y'GGCC_r
Cuts at: 1016 1190 1581 1608 2953 1016
Size: 174 391 27 1345 1494
Fragments arranged by size:
1494 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1016 1016
Size: 3431
EarI CTCTTCn'nnn_
Cuts at: 235 1454 1664 235
Size: 1219 210 2002
Fragments arranged by size:
2002 1219 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1639 1949 2095 3241 1639
Size: 310 146 1146 1829
Fragments arranged by size:
1829 1146 310 146
EcoICRI GAG'CTC
Cuts at: 443 443
Size: 3431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1256 1688 2435 2703 1256
Size: 432 747 268 1984
Fragments arranged by size:
1984 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 355 570 355
Size: 215 3216
EcoRI G'AATT_C
Cuts at: 435 435
Size: 3431
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1450 1450
Size: 3431
FspI TGC'GCA
Cuts at: 1212 1212
Size: 3431
HaeII r_GCGC'y
Cuts at: 865 1113 2135 3114 865
Size: 248 1022 979 1182
Fragments arranged by size:
1182 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1273 1303 1305 1335 1273
Size: 30 2 30 3369
Fragments arranged by size:
3369 30 30 2
HincII GTy'rAC
Cuts at: 470 470
Size: 3431
KasI G'GCGC_C
Cuts at: 861 1109 861
Size: 248 3183
KpnI G_GTAC'C
Cuts at: 451 451
Size: 3431
MluI A'CGCG_T
Cuts at: 2864 2864
Size: 3431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 168 281 2102 2286 168
Size: 113 1821 184 1313
Fragments arranged by size:
1821 1313 184 113
MscI TGG'CCA
Cuts at: 1192 1192
Size: 3431
MslI CAynn'nnrTG
Cuts at: 1420 1547 1420
Size: 127 3304
MspA1I CmG'CkG
Cuts at: 121 542 856 1216 2229 2474 121
Size: 421 314 360 1013 245 1078
Fragments arranged by size:
1078 1013 421 360 314 245
NaeI GCC'GGC
Cuts at: 1612 1612
Size: 3431
NarI GG'CG_CC
Cuts at: 862 1110 862
Size: 248 3183
NgoMIV G'CCGG_C
Cuts at: 1610 1610
Size: 3431
NspI r_CATG'y
Cuts at: 484 984 1891 484
Size: 500 907 2024
Fragments arranged by size:
2024 907 500
PciI A'CATG_T
Cuts at: 1887 1887
Size: 3431
PflMI CCAn_nnn'nTGG
Cuts at: 365 365
Size: 3431
PmeI GTTT'AAAC
Cuts at: 430 430
Size: 3431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2570 2602 2570
Size: 32 3399
PpuMI rG'GwC_Cy
Cuts at: 355 355
Size: 3431
PstI C_TGCA'G
Cuts at: 478 1163 478
Size: 685 2746
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3431
PvuII CAG'CTG
Cuts at: 856 1216 856
Size: 360 3071
RsrII CG'GwC_CG
Cuts at: 1626 1626
Size: 3431
SacI G_AGCT'C
Cuts at: 445 445
Size: 3431
SacII CC_GC'GG
Cuts at: 122 122
Size: 3431
SalI G'TCGA_C
Cuts at: 468 468
Size: 3431
SapI GCTCTTCn'nnn_
Cuts at: 1454 1664 1454
Size: 210 3221
SbfI CC_TGCA'GG
Cuts at: 478 478
Size: 3431
ScaI AGT'ACT
Cuts at: 2948 2948
Size: 3431
SfcI C'TryA_G
Cuts at: 33 474 1159 2152 2343 33
Size: 441 685 993 191 1121
Fragments arranged by size:
1121 993 685 441 191
SfoI GGC'GCC
Cuts at: 863 1111 863
Size: 248 3183
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3431
SmaI CCC'GGG
Cuts at: 453 453
Size: 3431
SmlI C'TyrA_G
Cuts at: 409 586 1501 1993 2255 409
Size: 177 915 492 262 1585
Fragments arranged by size:
1585 915 492 262 177
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3431
SphI G_CATG'C
Cuts at: 484 984 484
Size: 500 2931
SspI AAT'ATT
Cuts at: 348 348
Size: 3431
StuI AGG'CCT
Cuts at: 363 363
Size: 3431
StyI C'CwwG_G
Cuts at: 364 387 565 919 364
Size: 23 178 354 2876
Fragments arranged by size:
2876 354 178 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1014 1147 1606 1815 1014
Size: 133 459 209 2630
Fragments arranged by size:
2630 459 209 133
TatI w'GTAC_w
Cuts at: 2946 2946
Size: 3431
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 3399
Tth111I GACn'n_nGTC
Cuts at: 1228 1228
Size: 3431
XbaI T'CTAG_A
Cuts at: 462 462
Size: 3431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 926 926
Size: 3431
XmaI C'CCGG_G
Cuts at: 451 451
Size: 3431
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI