Restriction map for
pF1KB6758
(Circular) (Six-base) MAPSORT of: pF1KB6758.seq Check: 7880 from: 1 to: 3554
pF1KB6758 3554 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 592 592
Size: 3554
Acc65I G'GTAC_C
Cuts at: 570 570
Size: 3554
AclI AA'CG_TT
Cuts at: 3394 3394
Size: 3554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1379 1811 2558 2826 184
Size: 1195 432 747 268 912
Fragments arranged by size:
1195 912 747 432 268
AflIII A'CryG_T
Cuts at: 906 2010 2987 906
Size: 1104 977 1473
Fragments arranged by size:
1473 1104 977
AgeI A'CCGG_T
Cuts at: 1942 1942
Size: 3554
AlwNI CAG_nnn'CTG
Cuts at: 859 2426 859
Size: 1567 1987
ApaLI G'TGCA_C
Cuts at: 2324 2324
Size: 3554
ApoI r'AATT_y
Cuts at: 558 2907 3163 558
Size: 2349 256 949
Fragments arranged by size:
2349 949 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3554
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3554
AvaI C'yCGr_G
Cuts at: 574 574
Size: 3554
BamHI G'GATC_C
Cuts at: 579 579
Size: 3554
BanI G'GyrC_C
Cuts at: 511 570 984 1232 1267 511
Size: 59 414 248 35 2798
Fragments arranged by size:
2798 414 248 59 35
BanII G_rGCy'C
Cuts at: 568 1598 568
Size: 1030 2524
BbeI G_GCGC'C
Cuts at: 988 1236 988
Size: 248 3306
BbsI GAAGACnn'nnnn_
Cuts at: 145 145
Size: 3554
BbvCI CC'TCA_GC
Cuts at: 483 483
Size: 3554
BciVI GTATCCnnnnn_n'
Cuts at: 1452 2213 3554 1452
Size: 761 1341 1452
Fragments arranged by size:
1452 1341 761
BclI T'GATC_A
Cuts at: 1074 1074
Size: 3554
BglII A'GATC_T
Cuts at: 1069 3106 1069
Size: 2037 1517
BlpI GC'TnA_GC
Cuts at: 223 666 223
Size: 443 3111
Bme1580I G_kGCm'C
Cuts at: 516 1179 1272 2328 516
Size: 663 93 1056 1742
Fragments arranged by size:
1742 1056 663 93
BmrI ACTGGGnnnn_n'
Cuts at: 766 847 1182 3326 766
Size: 81 335 2144 994
Fragments arranged by size:
2144 994 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1921 1921
Size: 3554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 305
Size: 3554
Bpu10I CC'TnA_GC
Cuts at: 483 483
Size: 3554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1609 2101 2399 730
Size: 879 492 298 1885
Fragments arranged by size:
1885 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3258 3258
Size: 3554
BsaAI yAC'GTr
Cuts at: 907 907
Size: 3554
BsaBI GATnn'nnATC
Cuts at: 6 1068 3105 6
Size: 1062 2037 455
Fragments arranged by size:
2037 1062 455
BsaHI Gr'CG_yC
Cuts at: 985 1233 1935 3431 985
Size: 248 702 1496 1108
Fragments arranged by size:
1496 1108 702 248
BsaWI w'CCGG_w
Cuts at: 743 1264 1942 2216 2363 2774 743
Size: 521 678 274 147 411 1523
Fragments arranged by size:
1523 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 303 333 508 538 303
Size: 30 175 30 3319
Fragments arranged by size:
3319 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 130
Size: 3554
BseYI C'CCAG_C
Cuts at: 499 979 1737 2314 2810 499
Size: 480 758 577 496 1243
Fragments arranged by size:
1243 758 577 496 480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 214
Size: 3554
BsiEI CG_ry'CG
Cuts at: 104 1096 1142 2350 104
Size: 992 46 1208 1308
Fragments arranged by size:
1308 1208 992 46
BsiHKAI G_wGCw'C
Cuts at: 568 1346 1536 2328 568
Size: 778 190 792 1794
Fragments arranged by size:
1794 792 778 190
BsmI GAATG_Cn'
Cuts at: 422 422
Size: 3554
BsmBI CGTCTCn'nnnn_
Cuts at: 379 379
Size: 3554
Bsp1286I G_dGCh'C
Cuts at: 516 568 1179 1272 1346 1536 1598 2328 516
Size: 52 611 93 74 190 62 730 1742
Fragments arranged by size:
1742 730 611 190 93 74 62 52
BspEI T'CCGG_A
Cuts at: 2774 2774
Size: 3554
BspHI T'CATG_A
Cuts at: 2730 2730
Size: 3554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 604 818 1120 1501 604
Size: 214 302 381 2657
Fragments arranged by size:
2657 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1846 1900 3384 3549 50
Size: 1796 54 1484 165 55
Fragments arranged by size:
1796 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1466 1466
Size: 3554
BsrFI r'CCGG_y
Cuts at: 1552 1733 1942 1552
Size: 181 209 3164
Fragments arranged by size:
3164 209 181
BssSI C'ACGA_G
Cuts at: 1825 2183 1825
Size: 358 3196
BstAPI GCAn_nnn'nTGC
Cuts at: 452 931 452
Size: 479 3075
BstBI TT'CG_AA
Cuts at: 1915 1915
Size: 3554
BstXI CCAn_nnnn'nTGG
Cuts at: 499 499
Size: 3554
BstYI r'GATC_y
Cuts at: 1 579 1046 1069 1404 1650 2662 2748 2760
Size: 578 467 23 335 246 1012 86 12
Cuts at: 2760 3106 1
Size: 346 449
Fragments arranged by size:
1012 578 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 188 188
Size: 3554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1504 1685 1848 1504
Size: 181 163 3210
Fragments arranged by size:
3210 181 163
BtsI GCAGTG_nn'
Cuts at: 427 797 427
Size: 370 3184
DraI TTT'AAA
Cuts at: 553 553
Size: 3554
DrdI GACnn_nn'nnGTC
Cuts at: 1260 2118 1260
Size: 858 2696
EaeI y'GGCC_r
Cuts at: 170 1139 1313 1704 1731 3076 170
Size: 969 174 391 27 1345 648
Fragments arranged by size:
1345 969 648 391 174 27
EagI C'GGCC_G
Cuts at: 1139 1139
Size: 3554
EarI CTCTTCn'nnn_
Cuts at: 1577 1787 1577
Size: 210 3344
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1762 2072 2218 3364 1762
Size: 310 146 1146 1952
Fragments arranged by size:
1952 1146 310 146
EcoICRI GAG'CTC
Cuts at: 566 566
Size: 3554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 305 1379 1811 2558 2826 184
Size: 121 1074 432 747 268 912
Fragments arranged by size:
1074 912 747 432 268 121
EcoO109I rG'GnC_Cy
Cuts at: 693 693
Size: 3554
EcoRI G'AATT_C
Cuts at: 558 558
Size: 3554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1573 1573
Size: 3554
FspI TGC'GCA
Cuts at: 1335 1335
Size: 3554
HaeII r_GCGC'y
Cuts at: 988 1236 2258 3237 988
Size: 248 1022 979 1305
Fragments arranged by size:
1305 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 378 408 410 1396 1426 1428 1458 376
Size: 2 30 2 986 30 2 30 2472
Fragments arranged by size:
2472 986 30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 593 593
Size: 3554
KasI G'GCGC_C
Cuts at: 984 1232 984
Size: 248 3306
KpnI G_GTAC'C
Cuts at: 574 574
Size: 3554
MluI A'CGCG_T
Cuts at: 2987 2987
Size: 3554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2225 2409 2225
Size: 184 3370
MscI TGG'CCA
Cuts at: 1315 1315
Size: 3554
MslI CAynn'nnrTG
Cuts at: 1543 1670 1543
Size: 127 3427
MspA1I CmG'CkG
Cuts at: 222 665 979 1339 2352 2597 222
Size: 443 314 360 1013 245 1179
Fragments arranged by size:
1179 1013 443 360 314 245
NaeI GCC'GGC
Cuts at: 1735 1735
Size: 3554
NarI GG'CG_CC
Cuts at: 985 1233 985
Size: 248 3306
NgoMIV G'CCGG_C
Cuts at: 1733 1733
Size: 3554
NspI r_CATG'y
Cuts at: 607 1107 2014 607
Size: 500 907 2147
Fragments arranged by size:
2147 907 500
PciI A'CATG_T
Cuts at: 2010 2010
Size: 3554
PmeI GTTT'AAAC
Cuts at: 553 553
Size: 3554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2693 2725 2693
Size: 32 3522
PstI C_TGCA'G
Cuts at: 601 1286 601
Size: 685 2869
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3554
PvuII CAG'CTG
Cuts at: 979 1339 979
Size: 360 3194
RsrII CG'GwC_CG
Cuts at: 1749 1749
Size: 3554
SacI G_AGCT'C
Cuts at: 568 568
Size: 3554
SalI G'TCGA_C
Cuts at: 591 591
Size: 3554
SapI GCTCTTCn'nnn_
Cuts at: 1577 1787 1577
Size: 210 3344
SbfI CC_TGCA'GG
Cuts at: 601 601
Size: 3554
ScaI AGT'ACT
Cuts at: 3071 3071
Size: 3554
SfcI C'TryA_G
Cuts at: 33 506 597 1282 2275 2466 33
Size: 473 91 685 993 191 1121
Fragments arranged by size:
1121 993 685 473 191 91
SfoI GGC'GCC
Cuts at: 986 1234 986
Size: 248 3306
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3554
SmaI CCC'GGG
Cuts at: 576 576
Size: 3554
SmlI C'TyrA_G
Cuts at: 709 1624 2116 2378 709
Size: 915 492 262 1885
Fragments arranged by size:
1885 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3554
SphI G_CATG'C
Cuts at: 607 1107 607
Size: 500 3054
StuI AGG'CCT
Cuts at: 241 241
Size: 3554
StyI C'CwwG_G
Cuts at: 125 688 1042 125
Size: 563 354 2637
Fragments arranged by size:
2637 563 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1137 1270 1729 1938 1137
Size: 133 459 209 2753
Fragments arranged by size:
2753 459 209 133
TatI w'GTAC_w
Cuts at: 3069 3069
Size: 3554
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 495 508 527 540 3071 3103 495
Size: 13 19 13 2531 32 946
Fragments arranged by size:
2531 946 32 19 13 13
Tth111I GACn'n_nGTC
Cuts at: 390 1351 390
Size: 961 2593
XbaI T'CTAG_A
Cuts at: 585 585
Size: 3554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1049 1049
Size: 3554
XmaI C'CCGG_G
Cuts at: 574 574
Size: 3554
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI