Restriction map for
pF1KB6761
(Circular) (Six-base) MAPSORT of: pF1KB6761.seq Check: 740 from: 1 to: 3440
pF1KB6761 3440 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 478 478
Size: 3440
Acc65I G'GTAC_C
Cuts at: 456 456
Size: 3440
AclI AA'CG_TT
Cuts at: 3280 3280
Size: 3440
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1265 1697 2444 2712 1265
Size: 432 747 268 1993
Fragments arranged by size:
1993 747 432 268
AflIII A'CryG_T
Cuts at: 792 1896 2873 792
Size: 1104 977 1359
Fragments arranged by size:
1359 1104 977
AgeI A'CCGG_T
Cuts at: 1828 1828
Size: 3440
AleI CACnn'nnGTG
Cuts at: 159 378 159
Size: 219 3221
AlwNI CAG_nnn'CTG
Cuts at: 181 279 745 2312 181
Size: 98 466 1567 1309
Fragments arranged by size:
1567 1309 466 98
ApaLI G'TGCA_C
Cuts at: 2210 2210
Size: 3440
ApoI r'AATT_y
Cuts at: 444 2793 3049 444
Size: 2349 256 835
Fragments arranged by size:
2349 835 256
AseI AT'TA_AT
Cuts at: 20 221 20
Size: 201 3239
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3440
AvaI C'yCGr_G
Cuts at: 317 460 317
Size: 143 3297
BamHI G'GATC_C
Cuts at: 465 465
Size: 3440
BanI G'GyrC_C
Cuts at: 456 870 1118 1153 456
Size: 414 248 35 2743
Fragments arranged by size:
2743 414 248 35
BanII G_rGCy'C
Cuts at: 454 1484 454
Size: 1030 2410
BbeI G_GCGC'C
Cuts at: 874 1122 874
Size: 248 3192
BciVI GTATCCnnnnn_n'
Cuts at: 1338 2099 3440 1338
Size: 761 1341 1338
Fragments arranged by size:
1341 1338 761
BclI T'GATC_A
Cuts at: 960 960
Size: 3440
BglII A'GATC_T
Cuts at: 356 955 2992 356
Size: 599 2037 804
Fragments arranged by size:
2037 804 599
BlpI GC'TnA_GC
Cuts at: 206 552 206
Size: 346 3094
Bme1580I G_kGCm'C
Cuts at: 1065 1158 2214 1065
Size: 93 1056 2291
Fragments arranged by size:
2291 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 652 733 1068 3212 652
Size: 81 335 2144 880
Fragments arranged by size:
2144 880 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 3440
Bpu10I CC'TnA_GC
Cuts at: 269 269
Size: 3440
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1495 1987 2285 616
Size: 879 492 298 1771
Fragments arranged by size:
1771 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3144 3144
Size: 3440
BsaAI yAC'GTr
Cuts at: 793 793
Size: 3440
BsaBI GATnn'nnATC
Cuts at: 6 954 2991 6
Size: 948 2037 455
Fragments arranged by size:
2037 948 455
BsaHI Gr'CG_yC
Cuts at: 871 1119 1821 3317 871
Size: 248 702 1496 994
Fragments arranged by size:
1496 994 702 248
BsaWI w'CCGG_w
Cuts at: 629 1150 1828 2102 2249 2660 629
Size: 521 678 274 147 411 1409
Fragments arranged by size:
1409 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 408 438 408
Size: 30 3410
BseYI C'CCAG_C
Cuts at: 865 1623 2200 2696 865
Size: 758 577 496 1609
Fragments arranged by size:
1609 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 982 1028 2236 104
Size: 878 46 1208 1308
Fragments arranged by size:
1308 1208 878 46
BsiHKAI G_wGCw'C
Cuts at: 454 1232 1422 2214 454
Size: 778 190 792 1680
Fragments arranged by size:
1680 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 454 1065 1158 1232 1422 1484 2214 454
Size: 611 93 74 190 62 730 1680
Fragments arranged by size:
1680 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2660 2660
Size: 3440
BspHI T'CATG_A
Cuts at: 2616 2616
Size: 3440
BspMI ACCTGCnnnn'nnnn_
Cuts at: 244 490 704 1006 1387 244
Size: 246 214 302 381 2297
Fragments arranged by size:
2297 381 302 246 214
BsrBI CCG'CTC
Cuts at: 50 1732 1786 3270 3435 50
Size: 1682 54 1484 165 55
Fragments arranged by size:
1682 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1352 1352
Size: 3440
BsrFI r'CCGG_y
Cuts at: 1438 1619 1828 1438
Size: 181 209 3050
Fragments arranged by size:
3050 209 181
BssSI C'ACGA_G
Cuts at: 1711 2069 1711
Size: 358 3082
BstAPI GCAn_nnn'nTGC
Cuts at: 817 817
Size: 3440
BstBI TT'CG_AA
Cuts at: 1801 1801
Size: 3440
BstYI r'GATC_y
Cuts at: 1 356 465 932 955 1290 1536 2548 2634
Size: 355 109 467 23 335 246 1012 86
Cuts at: 2634 2646 2992 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 355 346 335 246 109
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1390 1571 1734 1390
Size: 181 163 3096
Fragments arranged by size:
3096 181 163
BtsI GCAGTG_nn'
Cuts at: 683 683
Size: 3440
DraI TTT'AAA
Cuts at: 439 439
Size: 3440
DrdI GACnn_nn'nnGTC
Cuts at: 1146 2004 1146
Size: 858 2582
EaeI y'GGCC_r
Cuts at: 1025 1199 1590 1617 2962 1025
Size: 174 391 27 1345 1503
Fragments arranged by size:
1503 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1025 1025
Size: 3440
EarI CTCTTCn'nnn_
Cuts at: 1463 1673 1463
Size: 210 3230
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1648 1958 2104 3250 1648
Size: 310 146 1146 1838
Fragments arranged by size:
1838 1146 310 146
EcoICRI GAG'CTC
Cuts at: 452 452
Size: 3440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1265 1697 2444 2712 301
Size: 964 432 747 268 1029
Fragments arranged by size:
1029 964 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 579 579
Size: 3440
EcoRI G'AATT_C
Cuts at: 444 444
Size: 3440
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1459 1459
Size: 3440
FspI TGC'GCA
Cuts at: 1221 1221
Size: 3440
HaeII r_GCGC'y
Cuts at: 874 1122 2144 3123 874
Size: 248 1022 979 1191
Fragments arranged by size:
1191 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1282 1312 1314 1344 1282
Size: 30 2 30 3378
Fragments arranged by size:
3378 30 30 2
HincII GTy'rAC
Cuts at: 479 479
Size: 3440
HindIII A'AGCT_T
Cuts at: 430 430
Size: 3440
KasI G'GCGC_C
Cuts at: 870 1118 870
Size: 248 3192
KpnI G_GTAC'C
Cuts at: 460 460
Size: 3440
MluI A'CGCG_T
Cuts at: 2873 2873
Size: 3440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 158 2111 2295 158
Size: 1953 184 1303
Fragments arranged by size:
1953 1303 184
MscI TGG'CCA
Cuts at: 1201 1201
Size: 3440
MslI CAynn'nnrTG
Cuts at: 159 378 1429 1556 159
Size: 219 1051 127 2043
Fragments arranged by size:
2043 1051 219 127
MspA1I CmG'CkG
Cuts at: 181 551 865 1225 2238 2483 181
Size: 370 314 360 1013 245 1138
Fragments arranged by size:
1138 1013 370 360 314 245
NaeI GCC'GGC
Cuts at: 1621 1621
Size: 3440
NarI GG'CG_CC
Cuts at: 871 1119 871
Size: 248 3192
NgoMIV G'CCGG_C
Cuts at: 1619 1619
Size: 3440
NspI r_CATG'y
Cuts at: 493 993 1900 493
Size: 500 907 2033
Fragments arranged by size:
2033 907 500
PasI CC'CwG_GG
Cuts at: 195 195
Size: 3440
PciI A'CATG_T
Cuts at: 1896 1896
Size: 3440
PfoI T'CCnGG_A
Cuts at: 386 386
Size: 3440
PmeI GTTT'AAAC
Cuts at: 439 439
Size: 3440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2579 2611 2579
Size: 32 3408
PstI C_TGCA'G
Cuts at: 153 487 1172 153
Size: 334 685 2421
Fragments arranged by size:
2421 685 334
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3440
PvuII CAG'CTG
Cuts at: 181 865 1225 181
Size: 684 360 2396
Fragments arranged by size:
2396 684 360
RsrII CG'GwC_CG
Cuts at: 1635 1635
Size: 3440
SacI G_AGCT'C
Cuts at: 454 454
Size: 3440
SalI G'TCGA_C
Cuts at: 477 477
Size: 3440
SapI GCTCTTCn'nnn_
Cuts at: 1463 1673 1463
Size: 210 3230
SbfI CC_TGCA'GG
Cuts at: 487 487
Size: 3440
ScaI AGT'ACT
Cuts at: 2957 2957
Size: 3440
SfcI C'TryA_G
Cuts at: 33 149 243 288 483 1168 2161 2352 33
Size: 116 94 45 195 685 993 191 1121
Fragments arranged by size:
1121 993 685 195 191 116 94 45
SfoI GGC'GCC
Cuts at: 872 1120 872
Size: 248 3192
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3440
SmaI CCC'GGG
Cuts at: 319 462 319
Size: 143 3297
SmlI C'TyrA_G
Cuts at: 595 1510 2002 2264 595
Size: 915 492 262 1771
Fragments arranged by size:
1771 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3440
SphI G_CATG'C
Cuts at: 493 993 493
Size: 500 2940
StyI C'CwwG_G
Cuts at: 574 928 574
Size: 354 3086
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1023 1156 1615 1824 1023
Size: 133 459 209 2639
Fragments arranged by size:
2639 459 209 133
TatI w'GTAC_w
Cuts at: 2955 2955
Size: 3440
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 3408
Tth111I GACn'n_nGTC
Cuts at: 1237 1237
Size: 3440
XbaI T'CTAG_A
Cuts at: 471 471
Size: 3440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 935 935
Size: 3440
XmaI C'CCGG_G
Cuts at: 317 460 317
Size: 143 3297
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI