Restriction map for
pF1KB6765
(Circular) (Six-base) MAPSORT of: pF1KB6765.seq Check: 8549 from: 1 to: 3455
pF1KB6765 3455 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 297 297
Size: 3455
AccI GT'mk_AC
Cuts at: 493 493
Size: 3455
Acc65I G'GTAC_C
Cuts at: 471 471
Size: 3455
AclI AA'CG_TT
Cuts at: 449 3295 449
Size: 2846 609
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 1280 1712 2459 2727 379
Size: 901 432 747 268 1107
Fragments arranged by size:
1107 901 747 432 268
AfeI AGC'GCT
Cuts at: 216 216
Size: 3455
AflIII A'CryG_T
Cuts at: 264 807 1911 2888 264
Size: 543 1104 977 831
Fragments arranged by size:
1104 977 831 543
AgeI A'CCGG_T
Cuts at: 1843 1843
Size: 3455
AlwNI CAG_nnn'CTG
Cuts at: 760 2327 760
Size: 1567 1888
ApaI G_GGCC'C
Cuts at: 206 206
Size: 3455
ApaLI G'TGCA_C
Cuts at: 2225 2225
Size: 3455
ApoI r'AATT_y
Cuts at: 422 459 2808 3064 422
Size: 37 2349 256 813
Fragments arranged by size:
2349 813 256 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 3455
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3455
AvaI C'yCGr_G
Cuts at: 141 475 141
Size: 334 3121
BamHI G'GATC_C
Cuts at: 480 480
Size: 3455
BanI G'GyrC_C
Cuts at: 471 885 1133 1168 471
Size: 414 248 35 2758
Fragments arranged by size:
2758 414 248 35
BanII G_rGCy'C
Cuts at: 157 206 469 1499 157
Size: 49 263 1030 2113
Fragments arranged by size:
2113 1030 263 49
BbeI G_GCGC'C
Cuts at: 889 1137 889
Size: 248 3207
BciVI GTATCCnnnnn_n'
Cuts at: 1353 2114 3455 1353
Size: 761 1341 1353
Fragments arranged by size:
1353 1341 761
BclI T'GATC_A
Cuts at: 975 975
Size: 3455
BglI GCCn_nnn'nGGC
Cuts at: 201 201
Size: 3455
BglII A'GATC_T
Cuts at: 970 3007 970
Size: 2037 1418
BlpI GC'TnA_GC
Cuts at: 567 567
Size: 3455
Bme1580I G_kGCm'C
Cuts at: 206 1080 1173 2229 206
Size: 874 93 1056 1432
Fragments arranged by size:
1432 1056 874 93
BmrI ACTGGGnnnn_n'
Cuts at: 667 748 1083 3227 667
Size: 81 335 2144 895
Fragments arranged by size:
2144 895 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1822 1822
Size: 3455
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1510 2002 2300 631
Size: 879 492 298 1786
Fragments arranged by size:
1786 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3159 3159
Size: 3455
BsaAI yAC'GTr
Cuts at: 808 808
Size: 3455
BsaBI GATnn'nnATC
Cuts at: 6 969 3006 6
Size: 963 2037 455
Fragments arranged by size:
2037 963 455
BsaHI Gr'CG_yC
Cuts at: 193 886 1134 1836 3332 193
Size: 693 248 702 1496 316
Fragments arranged by size:
1496 702 693 316 248
BsaWI w'CCGG_w
Cuts at: 644 1165 1843 2117 2264 2675 644
Size: 521 678 274 147 411 1424
Fragments arranged by size:
1424 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 880 1638 2215 2711 880
Size: 758 577 496 1624
Fragments arranged by size:
1624 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 997 1043 2251 104
Size: 893 46 1208 1308
Fragments arranged by size:
1308 1208 893 46
BsiHKAI G_wGCw'C
Cuts at: 334 469 1247 1437 2229 334
Size: 135 778 190 792 1560
Fragments arranged by size:
1560 792 778 190 135
Bsp1286I G_dGCh'C
Cuts at: 157 206 334 469 1080 1173 1247 1437 1499
Size: 49 128 135 611 93 74 190 62
Cuts at: 1499 2229 157
Size: 730 1383
Fragments arranged by size:
1383 730 611 190 135 128 93 74
62 49
BspEI T'CCGG_A
Cuts at: 2675 2675
Size: 3455
BspHI T'CATG_A
Cuts at: 2631 2631
Size: 3455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 297 505 719 1021 1402 297
Size: 208 214 302 381 2350
Fragments arranged by size:
2350 381 302 214 208
BsrBI CCG'CTC
Cuts at: 50 1747 1801 3285 3450 50
Size: 1697 54 1484 165 55
Fragments arranged by size:
1697 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1367 1367
Size: 3455
BsrFI r'CCGG_y
Cuts at: 1453 1634 1843 1453
Size: 181 209 3065
Fragments arranged by size:
3065 209 181
BssHII G'CGCG_C
Cuts at: 164 164
Size: 3455
BssSI C'ACGA_G
Cuts at: 1726 2084 1726
Size: 358 3097
BstAPI GCAn_nnn'nTGC
Cuts at: 832 832
Size: 3455
BstBI TT'CG_AA
Cuts at: 1816 1816
Size: 3455
BstYI r'GATC_y
Cuts at: 1 480 947 970 1305 1551 2563 2649 2661
Size: 479 467 23 335 246 1012 86 12
Cuts at: 2661 3007 1
Size: 346 449
Fragments arranged by size:
1012 479 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1405 1586 1749 1405
Size: 181 163 3111
Fragments arranged by size:
3111 181 163
BtsI GCAGTG_nn'
Cuts at: 334 698 334
Size: 364 3091
DraI TTT'AAA
Cuts at: 454 454
Size: 3455
DrdI GACnn_nn'nnGTC
Cuts at: 1161 2019 1161
Size: 858 2597
EaeI y'GGCC_r
Cuts at: 1040 1214 1605 1632 2977 1040
Size: 174 391 27 1345 1518
Fragments arranged by size:
1518 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1040 1040
Size: 3455
EarI CTCTTCn'nnn_
Cuts at: 1478 1688 1478
Size: 210 3245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1663 1973 2119 3265 1663
Size: 310 146 1146 1853
Fragments arranged by size:
1853 1146 310 146
EcoICRI GAG'CTC
Cuts at: 467 467
Size: 3455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 1280 1712 2459 2727 379
Size: 901 432 747 268 1107
Fragments arranged by size:
1107 901 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 145 202 203 594 145
Size: 57 1 391 3006
Fragments arranged by size:
3006 391 57 1
EcoRI G'AATT_C
Cuts at: 459 459
Size: 3455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1474 1474
Size: 3455
FspI TGC'GCA
Cuts at: 1236 1236
Size: 3455
HaeII r_GCGC'y
Cuts at: 218 889 1137 2159 3138 218
Size: 671 248 1022 979 535
Fragments arranged by size:
1022 979 671 535 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1297 1327 1329 1359 1297
Size: 30 2 30 3393
Fragments arranged by size:
3393 30 30 2
HincII GTy'rAC
Cuts at: 494 494
Size: 3455
KasI G'GCGC_C
Cuts at: 885 1133 885
Size: 248 3207
KpnI G_GTAC'C
Cuts at: 475 475
Size: 3455
MluI A'CGCG_T
Cuts at: 264 2888 264
Size: 2624 831
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2126 2310 2126
Size: 184 3271
MscI TGG'CCA
Cuts at: 1216 1216
Size: 3455
MslI CAynn'nnrTG
Cuts at: 1444 1571 1444
Size: 127 3328
MspA1I CmG'CkG
Cuts at: 566 880 1240 2253 2498 566
Size: 314 360 1013 245 1523
Fragments arranged by size:
1523 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1636 1636
Size: 3455
NarI GG'CG_CC
Cuts at: 886 1134 886
Size: 248 3207
NgoMIV G'CCGG_C
Cuts at: 1634 1634
Size: 3455
NspI r_CATG'y
Cuts at: 508 1008 1915 508
Size: 500 907 2048
Fragments arranged by size:
2048 907 500
PciI A'CATG_T
Cuts at: 1911 1911
Size: 3455
PmeI GTTT'AAAC
Cuts at: 454 454
Size: 3455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2594 2626 2594
Size: 32 3423
PpuMI rG'GwC_Cy
Cuts at: 145 145
Size: 3455
PspOMI G'GGCC_C
Cuts at: 202 202
Size: 3455
PstI C_TGCA'G
Cuts at: 308 502 1187 308
Size: 194 685 2576
Fragments arranged by size:
2576 685 194
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3455
PvuII CAG'CTG
Cuts at: 880 1240 880
Size: 360 3095
RsrII CG'GwC_CG
Cuts at: 1650 1650
Size: 3455
SacI G_AGCT'C
Cuts at: 469 469
Size: 3455
SalI G'TCGA_C
Cuts at: 492 492
Size: 3455
SapI GCTCTTCn'nnn_
Cuts at: 1478 1688 1478
Size: 210 3245
SbfI CC_TGCA'GG
Cuts at: 502 502
Size: 3455
ScaI AGT'ACT
Cuts at: 2972 2972
Size: 3455
SfcI C'TryA_G
Cuts at: 33 304 498 1183 2176 2367 33
Size: 271 194 685 993 191 1121
Fragments arranged by size:
1121 993 685 271 194 191
SfoI GGC'GCC
Cuts at: 887 1135 887
Size: 248 3207
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3455
SmaI CCC'GGG
Cuts at: 143 477 143
Size: 334 3121
SmlI C'TyrA_G
Cuts at: 610 1525 2017 2279 610
Size: 915 492 262 1786
Fragments arranged by size:
1786 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3455
SphI G_CATG'C
Cuts at: 508 1008 508
Size: 500 2955
StyI C'CwwG_G
Cuts at: 335 589 943 335
Size: 254 354 2847
Fragments arranged by size:
2847 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1038 1171 1630 1839 1038
Size: 133 459 209 2654
Fragments arranged by size:
2654 459 209 133
TatI w'GTAC_w
Cuts at: 2970 2970
Size: 3455
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 3423
Tth111I GACn'n_nGTC
Cuts at: 1252 1252
Size: 3455
XbaI T'CTAG_A
Cuts at: 486 486
Size: 3455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 342 950 342
Size: 608 2847
XmaI C'CCGG_G
Cuts at: 141 475 141
Size: 334 3121
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI