Restriction map for
pF1KB6766
(Circular) (Six-base) MAPSORT of: pF1KB6766.seq Check: 4920 from: 1 to: 3557
pF1KB6766 3557 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 495 595 495
Size: 100 3457
Acc65I G'GTAC_C
Cuts at: 573 573
Size: 3557
AclI AA'CG_TT
Cuts at: 3397 3397
Size: 3557
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1382 1814 2561 2829 328
Size: 1054 432 747 268 1056
Fragments arranged by size:
1056 1054 747 432 268
AflIII A'CryG_T
Cuts at: 909 2013 2990 909
Size: 1104 977 1476
Fragments arranged by size:
1476 1104 977
AgeI A'CCGG_T
Cuts at: 1945 1945
Size: 3557
AlwNI CAG_nnn'CTG
Cuts at: 318 862 2429 318
Size: 544 1567 1446
Fragments arranged by size:
1567 1446 544
ApaLI G'TGCA_C
Cuts at: 2327 2327
Size: 3557
ApoI r'AATT_y
Cuts at: 354 561 2910 3166 354
Size: 207 2349 256 745
Fragments arranged by size:
2349 745 256 207
AseI AT'TA_AT
Cuts at: 20 20
Size: 3557
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3557
AvaI C'yCGr_G
Cuts at: 164 577 164
Size: 413 3144
BamHI G'GATC_C
Cuts at: 582 582
Size: 3557
BanI G'GyrC_C
Cuts at: 159 573 987 1235 1270 159
Size: 414 414 248 35 2446
Fragments arranged by size:
2446 414 414 248 35
BanII G_rGCy'C
Cuts at: 125 571 1601 125
Size: 446 1030 2081
Fragments arranged by size:
2081 1030 446
BbeI G_GCGC'C
Cuts at: 163 991 1239 163
Size: 828 248 2481
Fragments arranged by size:
2481 828 248
BbsI GAAGACnn'nnnn_
Cuts at: 272 272
Size: 3557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3523
BciVI GTATCCnnnnn_n'
Cuts at: 1455 2216 3557 1455
Size: 761 1341 1455
Fragments arranged by size:
1455 1341 761
BclI T'GATC_A
Cuts at: 1077 1077
Size: 3557
BglI GCCn_nnn'nGGC
Cuts at: 168 168
Size: 3557
BglII A'GATC_T
Cuts at: 1072 3109 1072
Size: 2037 1520
BlpI GC'TnA_GC
Cuts at: 669 669
Size: 3557
Bme1580I G_kGCm'C
Cuts at: 251 1182 1275 2331 251
Size: 931 93 1056 1477
Fragments arranged by size:
1477 1056 931 93
BmrI ACTGGGnnnn_n'
Cuts at: 246 769 850 1185 3329 246
Size: 523 81 335 2144 474
Fragments arranged by size:
2144 523 474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 491
Size: 3557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1612 2104 2402 733
Size: 879 492 298 1888
Fragments arranged by size:
1888 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 370 3261 370
Size: 2891 666
BsaAI yAC'GTr
Cuts at: 910 910
Size: 3557
BsaBI GATnn'nnATC
Cuts at: 6 1071 3108 6
Size: 1065 2037 455
Fragments arranged by size:
2037 1065 455
BsaHI Gr'CG_yC
Cuts at: 160 988 1236 1938 3434 160
Size: 828 248 702 1496 283
Fragments arranged by size:
1496 828 702 283 248
BsaWI w'CCGG_w
Cuts at: 746 1267 1945 2219 2366 2777 746
Size: 521 678 274 147 411 1526
Fragments arranged by size:
1526 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 188 218 188
Size: 30 3527
BseYI C'CCAG_C
Cuts at: 360 536 982 1740 2317 2813 360
Size: 176 446 758 577 496 1104
Fragments arranged by size:
1104 758 577 496 446 176
BsiEI CG_ry'CG
Cuts at: 104 117 1099 1145 2353 104
Size: 13 982 46 1208 1308
Fragments arranged by size:
1308 1208 982 46 13
BsiHKAI G_wGCw'C
Cuts at: 125 271 571 1349 1539 2331 125
Size: 146 300 778 190 792 1351
Fragments arranged by size:
1351 792 778 300 190 146
Bsp1286I G_dGCh'C
Cuts at: 125 251 271 571 1182 1275 1349 1539 1601
Size: 126 20 300 611 93 74 190 62
Cuts at: 1601 2331 125
Size: 730 1351
Fragments arranged by size:
1351 730 611 300 190 126 93 74
62 20
BspEI T'CCGG_A
Cuts at: 2777 2777
Size: 3557
BspHI T'CATG_A
Cuts at: 2733 2733
Size: 3557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 607 821 1123 1504 607
Size: 214 302 381 2660
Fragments arranged by size:
2660 381 302 214
BsrBI CCG'CTC
Cuts at: 50 175 1849 1903 3387 3552 50
Size: 125 1674 54 1484 165 55
Fragments arranged by size:
1674 1484 165 125 55 54
BsrDI GCAATG_nn'
Cuts at: 1469 1469
Size: 3557
BsrFI r'CCGG_y
Cuts at: 1555 1736 1945 1555
Size: 181 209 3167
Fragments arranged by size:
3167 209 181
BssSI C'ACGA_G
Cuts at: 1828 2186 1828
Size: 358 3199
BstAPI GCAn_nnn'nTGC
Cuts at: 934 934
Size: 3557
BstBI TT'CG_AA
Cuts at: 1918 1918
Size: 3557
BstXI CCAn_nnnn'nTGG
Cuts at: 417 417
Size: 3557
BstYI r'GATC_y
Cuts at: 1 582 1049 1072 1407 1653 2665 2751 2763
Size: 581 467 23 335 246 1012 86 12
Cuts at: 2763 3109 1
Size: 346 449
Fragments arranged by size:
1012 581 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1507 1688 1851 1507
Size: 181 163 3213
Fragments arranged by size:
3213 181 163
BtsI GCAGTG_nn'
Cuts at: 800 800
Size: 3557
DraI TTT'AAA
Cuts at: 556 556
Size: 3557
DrdI GACnn_nn'nnGTC
Cuts at: 1263 2121 1263
Size: 858 2699
EaeI y'GGCC_r
Cuts at: 114 1142 1316 1707 1734 3079 114
Size: 1028 174 391 27 1345 592
Fragments arranged by size:
1345 1028 592 391 174 27
EagI C'GGCC_G
Cuts at: 114 1142 114
Size: 1028 2529
EarI CTCTTCn'nnn_
Cuts at: 1580 1790 1580
Size: 210 3347
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 209 1765 2075 2221 3367 209
Size: 1556 310 146 1146 399
Fragments arranged by size:
1556 1146 399 310 146
EcoICRI GAG'CTC
Cuts at: 123 569 123
Size: 446 3111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 491 1382 1814 2561 2829 328
Size: 163 891 432 747 268 1056
Fragments arranged by size:
1056 891 747 432 268 163
EcoO109I rG'GnC_Cy
Cuts at: 414 696 414
Size: 282 3275
EcoRI G'AATT_C
Cuts at: 561 561
Size: 3557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1576 1576
Size: 3557
FspI TGC'GCA
Cuts at: 1338 1338
Size: 3557
HaeII r_GCGC'y
Cuts at: 145 163 991 1239 2261 3240 145
Size: 18 828 248 1022 979 462
Fragments arranged by size:
1022 979 828 462 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 314 346 1399 1429 1431 1461 314
Size: 32 1053 30 2 30 2410
Fragments arranged by size:
2410 1053 32 30 30 2
HincII GTy'rAC
Cuts at: 596 596
Size: 3557
KasI G'GCGC_C
Cuts at: 159 987 1235 159
Size: 828 248 2481
Fragments arranged by size:
2481 828 248
KpnI G_GTAC'C
Cuts at: 577 577
Size: 3557
MluI A'CGCG_T
Cuts at: 2990 2990
Size: 3557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2412 2228
Size: 184 3373
MscI TGG'CCA
Cuts at: 1318 1318
Size: 3557
MslI CAynn'nnrTG
Cuts at: 1546 1673 1546
Size: 127 3430
MspA1I CmG'CkG
Cuts at: 129 183 318 540 668 982 1342 2355 2600
Size: 54 135 222 128 314 360 1013 245
Cuts at: 2600 129
Size: 1086
Fragments arranged by size:
1086 1013 360 314 245 222 135 128
54
NaeI GCC'GGC
Cuts at: 1738 1738
Size: 3557
NarI GG'CG_CC
Cuts at: 160 988 1236 160
Size: 828 248 2481
Fragments arranged by size:
2481 828 248
NgoMIV G'CCGG_C
Cuts at: 1736 1736
Size: 3557
NspI r_CATG'y
Cuts at: 610 1110 2017 610
Size: 500 907 2150
Fragments arranged by size:
2150 907 500
PasI CC'CwG_GG
Cuts at: 417 417
Size: 3557
PciI A'CATG_T
Cuts at: 2013 2013
Size: 3557
PmeI GTTT'AAAC
Cuts at: 556 556
Size: 3557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 3525
PstI C_TGCA'G
Cuts at: 604 1289 604
Size: 685 2872
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3557
PvuII CAG'CTG
Cuts at: 318 540 982 1342 318
Size: 222 442 360 2533
Fragments arranged by size:
2533 442 360 222
RsrII CG'GwC_CG
Cuts at: 1752 1752
Size: 3557
SacI G_AGCT'C
Cuts at: 125 571 125
Size: 446 3111
SalI G'TCGA_C
Cuts at: 594 594
Size: 3557
SapI GCTCTTCn'nnn_
Cuts at: 1580 1790 1580
Size: 210 3347
SbfI CC_TGCA'GG
Cuts at: 604 604
Size: 3557
ScaI AGT'ACT
Cuts at: 3074 3074
Size: 3557
SfcI C'TryA_G
Cuts at: 33 600 1285 2278 2469 33
Size: 567 685 993 191 1121
Fragments arranged by size:
1121 993 685 567 191
SfoI GGC'GCC
Cuts at: 161 989 1237 161
Size: 828 248 2481
Fragments arranged by size:
2481 828 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3557
SmaI CCC'GGG
Cuts at: 166 579 166
Size: 413 3144
SmlI C'TyrA_G
Cuts at: 712 1627 2119 2381 712
Size: 915 492 262 1888
Fragments arranged by size:
1888 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3557
SphI G_CATG'C
Cuts at: 610 1110 610
Size: 500 3057
StyI C'CwwG_G
Cuts at: 410 691 1045 410
Size: 281 354 2922
Fragments arranged by size:
2922 354 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1140 1273 1732 1941 1140
Size: 133 459 209 2756
Fragments arranged by size:
2756 459 209 133
TatI w'GTAC_w
Cuts at: 326 3072 326
Size: 2746 811
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 188 220 3074 3106 188
Size: 32 2854 32 639
Fragments arranged by size:
2854 639 32 32
Tth111I GACn'n_nGTC
Cuts at: 1354 1354
Size: 3557
XbaI T'CTAG_A
Cuts at: 588 588
Size: 3557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1052 1052
Size: 3557
XmaI C'CCGG_G
Cuts at: 164 577 164
Size: 413 3144
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI