Restriction map for
pF1KB6771
(Circular) (Six-base) MAPSORT of: pF1KB6771.seq Check: 1568 from: 1 to: 3467
pF1KB6771 3467 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 505 505
Size: 3467
Acc65I G'GTAC_C
Cuts at: 483 483
Size: 3467
AclI AA'CG_TT
Cuts at: 3307 3307
Size: 3467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 1292 1724 2471 2739 438
Size: 854 432 747 268 1166
Fragments arranged by size:
1166 854 747 432 268
AflIII A'CryG_T
Cuts at: 819 1923 2900 819
Size: 1104 977 1386
Fragments arranged by size:
1386 1104 977
AgeI A'CCGG_T
Cuts at: 1855 1855
Size: 3467
AlwNI CAG_nnn'CTG
Cuts at: 291 772 2339 291
Size: 481 1567 1419
Fragments arranged by size:
1567 1419 481
ApaLI G'TGCA_C
Cuts at: 2237 2237
Size: 3467
ApoI r'AATT_y
Cuts at: 471 2820 3076 471
Size: 2349 256 862
Fragments arranged by size:
2349 862 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
AvaI C'yCGr_G
Cuts at: 487 487
Size: 3467
BamHI G'GATC_C
Cuts at: 492 492
Size: 3467
BanI G'GyrC_C
Cuts at: 483 897 1145 1180 483
Size: 414 248 35 2770
Fragments arranged by size:
2770 414 248 35
BanII G_rGCy'C
Cuts at: 481 1511 481
Size: 1030 2437
BbeI G_GCGC'C
Cuts at: 901 1149 901
Size: 248 3219
BciVI GTATCCnnnnn_n'
Cuts at: 1365 2126 3467 1365
Size: 761 1341 1365
Fragments arranged by size:
1365 1341 761
BclI T'GATC_A
Cuts at: 401 987 401
Size: 586 2881
BglII A'GATC_T
Cuts at: 982 3019 982
Size: 2037 1430
BlpI GC'TnA_GC
Cuts at: 579 579
Size: 3467
Bme1580I G_kGCm'C
Cuts at: 1092 1185 2241 1092
Size: 93 1056 2318
Fragments arranged by size:
2318 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 333 679 760 1095 3239 333
Size: 346 81 335 2144 561
Fragments arranged by size:
2144 561 346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 307 295
Size: 12 3455
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 376 643 1522 2014 2312 349
Size: 27 267 879 492 298 1504
Fragments arranged by size:
1504 879 492 298 267 27
BsaI GGTCTCn'nnnn_
Cuts at: 275 3171 275
Size: 2896 571
BsaAI yAC'GTr
Cuts at: 820 820
Size: 3467
BsaBI GATnn'nnATC
Cuts at: 6 400 403 981 3018 6
Size: 394 3 578 2037 455
Fragments arranged by size:
2037 578 455 394 3
BsaHI Gr'CG_yC
Cuts at: 898 1146 1848 3344 898
Size: 248 702 1496 1021
Fragments arranged by size:
1496 1021 702 248
BsaWI w'CCGG_w
Cuts at: 656 1177 1855 2129 2276 2687 656
Size: 521 678 274 147 411 1436
Fragments arranged by size:
1436 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 154 892 1650 2227 2723 154
Size: 738 758 577 496 898
Fragments arranged by size:
898 758 738 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 332 158
Size: 174 3293
BsiEI CG_ry'CG
Cuts at: 104 309 1009 1055 2263 104
Size: 205 700 46 1208 1308
Fragments arranged by size:
1308 1208 700 205 46
BsiHKAI G_wGCw'C
Cuts at: 481 1259 1449 2241 481
Size: 778 190 792 1707
Fragments arranged by size:
1707 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 481 1092 1185 1259 1449 1511 2241 481
Size: 611 93 74 190 62 730 1707
Fragments arranged by size:
1707 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2687 2687
Size: 3467
BspHI T'CATG_A
Cuts at: 248 2643 248
Size: 2395 1072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 517 731 1033 1414 517
Size: 214 302 381 2570
Fragments arranged by size:
2570 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1759 1813 3297 3462 50
Size: 1709 54 1484 165 55
Fragments arranged by size:
1709 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1379 1379
Size: 3467
BsrFI r'CCGG_y
Cuts at: 1465 1646 1855 1465
Size: 181 209 3077
Fragments arranged by size:
3077 209 181
BssSI C'ACGA_G
Cuts at: 1738 2096 1738
Size: 358 3109
BstAPI GCAn_nnn'nTGC
Cuts at: 844 844
Size: 3467
BstBI TT'CG_AA
Cuts at: 1828 1828
Size: 3467
BstYI r'GATC_y
Cuts at: 1 492 959 982 1317 1563 2575 2661 2673
Size: 491 467 23 335 246 1012 86 12
Cuts at: 2673 3019 1
Size: 346 449
Fragments arranged by size:
1012 491 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 131 107
Size: 24 3443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1417 1598 1761 1417
Size: 181 163 3123
Fragments arranged by size:
3123 181 163
BtsI GCAGTG_nn'
Cuts at: 710 710
Size: 3467
DraI TTT'AAA
Cuts at: 466 466
Size: 3467
DrdI GACnn_nn'nnGTC
Cuts at: 1173 2031 1173
Size: 858 2609
EaeI y'GGCC_r
Cuts at: 1052 1226 1617 1644 2989 1052
Size: 174 391 27 1345 1530
Fragments arranged by size:
1530 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1052 1052
Size: 3467
EarI CTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1675 1985 2131 3277 1675
Size: 310 146 1146 1865
Fragments arranged by size:
1865 1146 310 146
EcoICRI GAG'CTC
Cuts at: 479 479
Size: 3467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 307 438 1292 1724 2471 2739 295
Size: 12 131 854 432 747 268 1023
Fragments arranged by size:
1023 854 747 432 268 131 12
EcoO109I rG'GnC_Cy
Cuts at: 270 332 606 270
Size: 62 274 3131
Fragments arranged by size:
3131 274 62
EcoRI G'AATT_C
Cuts at: 471 471
Size: 3467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1486 1486
Size: 3467
FspI TGC'GCA
Cuts at: 1248 1248
Size: 3467
HaeII r_GCGC'y
Cuts at: 901 1149 2171 3150 901
Size: 248 1022 979 1218
Fragments arranged by size:
1218 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1309 1339 1341 1371 1309
Size: 30 2 30 3405
Fragments arranged by size:
3405 30 30 2
HincII GTy'rAC
Cuts at: 506 506
Size: 3467
KasI G'GCGC_C
Cuts at: 897 1145 897
Size: 248 3219
KpnI G_GTAC'C
Cuts at: 487 487
Size: 3467
MluI A'CGCG_T
Cuts at: 2900 2900
Size: 3467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 275 2138 2322 275
Size: 1863 184 1420
Fragments arranged by size:
1863 1420 184
MscI TGG'CCA
Cuts at: 1228 1228
Size: 3467
MslI CAynn'nnrTG
Cuts at: 253 1456 1583 253
Size: 1203 127 2137
Fragments arranged by size:
2137 1203 127
MspA1I CmG'CkG
Cuts at: 127 578 892 1252 2265 2510 127
Size: 451 314 360 1013 245 1084
Fragments arranged by size:
1084 1013 451 360 314 245
NaeI GCC'GGC
Cuts at: 1648 1648
Size: 3467
NarI GG'CG_CC
Cuts at: 898 1146 898
Size: 248 3219
NcoI C'CATG_G
Cuts at: 107 107
Size: 3467
NgoMIV G'CCGG_C
Cuts at: 1646 1646
Size: 3467
NspI r_CATG'y
Cuts at: 520 1020 1927 520
Size: 500 907 2060
Fragments arranged by size:
2060 907 500
PciI A'CATG_T
Cuts at: 1923 1923
Size: 3467
PfoI T'CCnGG_A
Cuts at: 272 272
Size: 3467
PmeI GTTT'AAAC
Cuts at: 466 466
Size: 3467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 3435
PpuMI rG'GwC_Cy
Cuts at: 270 270
Size: 3467
PstI C_TGCA'G
Cuts at: 212 514 1199 212
Size: 302 685 2480
Fragments arranged by size:
2480 685 302
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3467
PvuII CAG'CTG
Cuts at: 892 1252 892
Size: 360 3107
RsrII CG'GwC_CG
Cuts at: 1662 1662
Size: 3467
SacI G_AGCT'C
Cuts at: 481 481
Size: 3467
SalI G'TCGA_C
Cuts at: 504 504
Size: 3467
SapI GCTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
SbfI CC_TGCA'GG
Cuts at: 514 514
Size: 3467
ScaI AGT'ACT
Cuts at: 2984 2984
Size: 3467
SfcI C'TryA_G
Cuts at: 33 208 510 1195 2188 2379 33
Size: 175 302 685 993 191 1121
Fragments arranged by size:
1121 993 685 302 191 175
SfoI GGC'GCC
Cuts at: 899 1147 899
Size: 248 3219
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
SmaI CCC'GGG
Cuts at: 489 489
Size: 3467
SmlI C'TyrA_G
Cuts at: 364 391 622 1537 2029 2291 364
Size: 27 231 915 492 262 1540
Fragments arranged by size:
1540 915 492 262 231 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3467
SphI G_CATG'C
Cuts at: 520 1020 520
Size: 500 2967
StyI C'CwwG_G
Cuts at: 107 601 955 107
Size: 494 354 2619
Fragments arranged by size:
2619 494 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1050 1183 1642 1851 1050
Size: 133 459 209 2666
Fragments arranged by size:
2666 459 209 133
TatI w'GTAC_w
Cuts at: 2982 2982
Size: 3467
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3435
Tth111I GACn'n_nGTC
Cuts at: 268 1264 268
Size: 996 2471
XbaI T'CTAG_A
Cuts at: 498 498
Size: 3467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 962 962
Size: 3467
XmaI C'CCGG_G
Cuts at: 487 487
Size: 3467
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI