Restriction map for
pF1KB6773
(Circular) (Six-base) MAPSORT of: pF1KB6773.seq Check: 5433 from: 1 to: 3467
pF1KB6773 3467 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 138 138
Size: 3467
AccI GT'mk_AC
Cuts at: 505 505
Size: 3467
Acc65I G'GTAC_C
Cuts at: 483 483
Size: 3467
AclI AA'CG_TT
Cuts at: 3307 3307
Size: 3467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 1292 1724 2471 2739 155
Size: 1137 432 747 268 883
Fragments arranged by size:
1137 883 747 432 268
AflIII A'CryG_T
Cuts at: 252 819 1923 2900 252
Size: 567 1104 977 819
Fragments arranged by size:
1104 977 819 567
AgeI A'CCGG_T
Cuts at: 1855 1855
Size: 3467
AlwNI CAG_nnn'CTG
Cuts at: 291 772 2339 291
Size: 481 1567 1419
Fragments arranged by size:
1567 1419 481
ApaLI G'TGCA_C
Cuts at: 2237 2237
Size: 3467
ApoI r'AATT_y
Cuts at: 471 2820 3076 471
Size: 2349 256 862
Fragments arranged by size:
2349 862 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
AvaI C'yCGr_G
Cuts at: 487 487
Size: 3467
BamHI G'GATC_C
Cuts at: 492 492
Size: 3467
BanI G'GyrC_C
Cuts at: 483 897 1145 1180 483
Size: 414 248 35 2770
Fragments arranged by size:
2770 414 248 35
BanII G_rGCy'C
Cuts at: 481 1511 481
Size: 1030 2437
BbeI G_GCGC'C
Cuts at: 901 1149 901
Size: 248 3219
BciVI GTATCCnnnnn_n'
Cuts at: 1365 2126 3467 1365
Size: 761 1341 1365
Fragments arranged by size:
1365 1341 761
BclI T'GATC_A
Cuts at: 401 987 401
Size: 586 2881
BglI GCCn_nnn'nGGC
Cuts at: 325 325
Size: 3467
BglII A'GATC_T
Cuts at: 982 3019 982
Size: 2037 1430
BlpI GC'TnA_GC
Cuts at: 122 579 122
Size: 457 3010
Bme1580I G_kGCm'C
Cuts at: 1092 1185 2241 1092
Size: 93 1056 2318
Fragments arranged by size:
2318 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 679 760 1095 3239 679
Size: 81 335 2144 907
Fragments arranged by size:
2144 907 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 295 101
Size: 194 3273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1522 2014 2312 643
Size: 879 492 298 1798
Fragments arranged by size:
1798 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3171 3171
Size: 3467
BsaAI yAC'GTr
Cuts at: 253 820 253
Size: 567 2900
BsaBI GATnn'nnATC
Cuts at: 6 400 981 3018 6
Size: 394 581 2037 455
Fragments arranged by size:
2037 581 455 394
BsaHI Gr'CG_yC
Cuts at: 898 1146 1848 3344 898
Size: 248 702 1496 1021
Fragments arranged by size:
1496 1021 702 248
BsaWI w'CCGG_w
Cuts at: 656 1177 1855 2129 2276 2687 656
Size: 521 678 274 147 411 1436
Fragments arranged by size:
1436 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 170 247 170
Size: 77 3390
BseYI C'CCAG_C
Cuts at: 408 892 1650 2227 2723 408
Size: 484 758 577 496 1152
Fragments arranged by size:
1152 758 577 496 484
BsiEI CG_ry'CG
Cuts at: 104 1009 1055 2263 104
Size: 905 46 1208 1308
Fragments arranged by size:
1308 1208 905 46
BsiHKAI G_wGCw'C
Cuts at: 481 1259 1449 2241 481
Size: 778 190 792 1707
Fragments arranged by size:
1707 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 481 1092 1185 1259 1449 1511 2241 481
Size: 611 93 74 190 62 730 1707
Fragments arranged by size:
1707 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2687 2687
Size: 3467
BspHI T'CATG_A
Cuts at: 2643 2643
Size: 3467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 138 517 731 1033 1414 138
Size: 379 214 302 381 2191
Fragments arranged by size:
2191 381 379 302 214
BsrBI CCG'CTC
Cuts at: 50 1759 1813 3297 3462 50
Size: 1709 54 1484 165 55
Fragments arranged by size:
1709 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1379 1379
Size: 3467
BsrFI r'CCGG_y
Cuts at: 1465 1646 1855 1465
Size: 181 209 3077
Fragments arranged by size:
3077 209 181
BssSI C'ACGA_G
Cuts at: 1738 2096 1738
Size: 358 3109
BstAPI GCAn_nnn'nTGC
Cuts at: 844 844
Size: 3467
BstBI TT'CG_AA
Cuts at: 1828 1828
Size: 3467
BstXI CCAn_nnnn'nTGG
Cuts at: 432 432
Size: 3467
BstYI r'GATC_y
Cuts at: 1 492 959 982 1317 1563 2575 2661 2673
Size: 491 467 23 335 246 1012 86 12
Cuts at: 2673 3019 1
Size: 346 449
Fragments arranged by size:
1012 491 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1417 1598 1761 1417
Size: 181 163 3123
Fragments arranged by size:
3123 181 163
BtsI GCAGTG_nn'
Cuts at: 357 710 357
Size: 353 3114
DraI TTT'AAA
Cuts at: 466 466
Size: 3467
DrdI GACnn_nn'nnGTC
Cuts at: 1173 2031 1173
Size: 858 2609
EaeI y'GGCC_r
Cuts at: 1052 1226 1617 1644 2989 1052
Size: 174 391 27 1345 1530
Fragments arranged by size:
1530 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1052 1052
Size: 3467
EarI CTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1675 1985 2131 3277 1675
Size: 310 146 1146 1865
Fragments arranged by size:
1865 1146 310 146
EcoICRI GAG'CTC
Cuts at: 479 479
Size: 3467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 155 295 1292 1724 2471 2739 101
Size: 54 140 997 432 747 268 829
Fragments arranged by size:
997 829 747 432 268 140 54
EcoNI CCTnn'n_nnAGG
Cuts at: 134 134
Size: 3467
EcoO109I rG'GnC_Cy
Cuts at: 166 270 606 166
Size: 104 336 3027
Fragments arranged by size:
3027 336 104
EcoRI G'AATT_C
Cuts at: 471 471
Size: 3467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1486 1486
Size: 3467
FspI TGC'GCA
Cuts at: 1248 1248
Size: 3467
HaeII r_GCGC'y
Cuts at: 901 1149 2171 3150 901
Size: 248 1022 979 1218
Fragments arranged by size:
1218 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1309 1339 1341 1371 1309
Size: 30 2 30 3405
Fragments arranged by size:
3405 30 30 2
HincII GTy'rAC
Cuts at: 506 506
Size: 3467
KasI G'GCGC_C
Cuts at: 897 1145 897
Size: 248 3219
KpnI G_GTAC'C
Cuts at: 487 487
Size: 3467
MluI A'CGCG_T
Cuts at: 2900 2900
Size: 3467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2138 2322 2138
Size: 184 3283
MscI TGG'CCA
Cuts at: 1228 1228
Size: 3467
MslI CAynn'nnrTG
Cuts at: 253 430 1456 1583 253
Size: 177 1026 127 2137
Fragments arranged by size:
2137 1026 177 127
MspA1I CmG'CkG
Cuts at: 578 892 1252 2265 2510 578
Size: 314 360 1013 245 1535
Fragments arranged by size:
1535 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1648 1648
Size: 3467
NarI GG'CG_CC
Cuts at: 898 1146 898
Size: 248 3219
NgoMIV G'CCGG_C
Cuts at: 1646 1646
Size: 3467
NspI r_CATG'y
Cuts at: 520 1020 1927 520
Size: 500 907 2060
Fragments arranged by size:
2060 907 500
PciI A'CATG_T
Cuts at: 1923 1923
Size: 3467
PfoI T'CCnGG_A
Cuts at: 272 272
Size: 3467
PmeI GTTT'AAAC
Cuts at: 466 466
Size: 3467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 3435
PpuMI rG'GwC_Cy
Cuts at: 166 270 166
Size: 104 3363
PstI C_TGCA'G
Cuts at: 514 1199 514
Size: 685 2782
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3467
PvuII CAG'CTG
Cuts at: 892 1252 892
Size: 360 3107
RsrII CG'GwC_CG
Cuts at: 1662 1662
Size: 3467
SacI G_AGCT'C
Cuts at: 481 481
Size: 3467
SalI G'TCGA_C
Cuts at: 504 504
Size: 3467
SanDI GG'GwC_CC
Cuts at: 166 166
Size: 3467
SapI GCTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
SbfI CC_TGCA'GG
Cuts at: 514 514
Size: 3467
ScaI AGT'ACT
Cuts at: 2984 2984
Size: 3467
SfcI C'TryA_G
Cuts at: 33 208 510 1195 2188 2379 33
Size: 175 302 685 993 191 1121
Fragments arranged by size:
1121 993 685 302 191 175
SfoI GGC'GCC
Cuts at: 899 1147 899
Size: 248 3219
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
SmaI CCC'GGG
Cuts at: 489 489
Size: 3467
SmlI C'TyrA_G
Cuts at: 622 1537 2029 2291 622
Size: 915 492 262 1798
Fragments arranged by size:
1798 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3467
SphI G_CATG'C
Cuts at: 520 1020 520
Size: 500 2967
StyI C'CwwG_G
Cuts at: 601 955 601
Size: 354 3113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 323 1050 1183 1642 1851 323
Size: 727 133 459 209 1939
Fragments arranged by size:
1939 727 459 209 133
TatI w'GTAC_w
Cuts at: 2982 2982
Size: 3467
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3435
Tth111I GACn'n_nGTC
Cuts at: 268 1264 268
Size: 996 2471
XbaI T'CTAG_A
Cuts at: 498 498
Size: 3467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 962 962
Size: 3467
XmaI C'CCGG_G
Cuts at: 487 487
Size: 3467
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI