Restriction map for
pF1KB6781
(Circular) (Six-base) MAPSORT of: pF1KB6781.seq Check: 4689 from: 1 to: 3473
pF1KB6781 3473 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 223 299 223
Size: 76 3397
AccI GT'mk_AC
Cuts at: 511 511
Size: 3473
Acc65I G'GTAC_C
Cuts at: 489 489
Size: 3473
AclI AA'CG_TT
Cuts at: 3313 3313
Size: 3473
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 1298 1730 2477 2745 244
Size: 1054 432 747 268 972
Fragments arranged by size:
1054 972 747 432 268
AflIII A'CryG_T
Cuts at: 825 1929 2906 825
Size: 1104 977 1392
Fragments arranged by size:
1392 1104 977
AgeI A'CCGG_T
Cuts at: 1861 1861
Size: 3473
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 193 225 193
Size: 32 3441
AlwNI CAG_nnn'CTG
Cuts at: 778 2345 778
Size: 1567 1906
ApaLI G'TGCA_C
Cuts at: 2243 2243
Size: 3473
ApoI r'AATT_y
Cuts at: 477 2826 3082 477
Size: 2349 256 868
Fragments arranged by size:
2349 868 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3473
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3473
AvaI C'yCGr_G
Cuts at: 493 493
Size: 3473
BamHI G'GATC_C
Cuts at: 375 498 375
Size: 123 3350
BanI G'GyrC_C
Cuts at: 489 903 1151 1186 489
Size: 414 248 35 2776
Fragments arranged by size:
2776 414 248 35
BanII G_rGCy'C
Cuts at: 487 1517 487
Size: 1030 2443
BbeI G_GCGC'C
Cuts at: 907 1155 907
Size: 248 3225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 314 348 314
Size: 34 3439
BciVI GTATCCnnnnn_n'
Cuts at: 259 1371 2132 3473 259
Size: 1112 761 1341 259
Fragments arranged by size:
1341 1112 761 259
BclI T'GATC_A
Cuts at: 993 993
Size: 3473
BglII A'GATC_T
Cuts at: 988 3025 988
Size: 2037 1436
BlpI GC'TnA_GC
Cuts at: 585 585
Size: 3473
Bme1580I G_kGCm'C
Cuts at: 1098 1191 2247 1098
Size: 93 1056 2324
Fragments arranged by size:
2324 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 363 685 766 1101 3245 363
Size: 322 81 335 2144 591
Fragments arranged by size:
2144 591 335 322 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1840 1840
Size: 3473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1528 2020 2318 649
Size: 879 492 298 1804
Fragments arranged by size:
1804 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 120 3177 120
Size: 3057 416
BsaAI yAC'GTr
Cuts at: 826 826
Size: 3473
BsaBI GATnn'nnATC
Cuts at: 6 987 3024 6
Size: 981 2037 455
Fragments arranged by size:
2037 981 455
BsaHI Gr'CG_yC
Cuts at: 904 1152 1854 3350 904
Size: 248 702 1496 1027
Fragments arranged by size:
1496 1027 702 248
BsaWI w'CCGG_w
Cuts at: 662 1183 1861 2135 2282 2693 662
Size: 521 678 274 147 411 1442
Fragments arranged by size:
1442 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 437 467 437
Size: 30 3443
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 445
Size: 3473
BseYI C'CCAG_C
Cuts at: 449 898 1656 2233 2729 449
Size: 449 758 577 496 1193
Fragments arranged by size:
1193 758 577 496 449
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 318
Size: 3473
BsiEI CG_ry'CG
Cuts at: 104 1015 1061 2269 104
Size: 911 46 1208 1308
Fragments arranged by size:
1308 1208 911 46
BsiHKAI G_wGCw'C
Cuts at: 487 1265 1455 2247 487
Size: 778 190 792 1713
Fragments arranged by size:
1713 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 487 1098 1191 1265 1455 1517 2247 487
Size: 611 93 74 190 62 730 1713
Fragments arranged by size:
1713 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2693 2693
Size: 3473
BspHI T'CATG_A
Cuts at: 2649 2649
Size: 3473
BspMI ACCTGCnnnn'nnnn_
Cuts at: 223 299 523 737 1039 1420 223
Size: 76 224 214 302 381 2276
Fragments arranged by size:
2276 381 302 224 214 76
BsrBI CCG'CTC
Cuts at: 50 1765 1819 3303 3468 50
Size: 1715 54 1484 165 55
Fragments arranged by size:
1715 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1385 1385
Size: 3473
BsrFI r'CCGG_y
Cuts at: 1471 1652 1861 1471
Size: 181 209 3083
Fragments arranged by size:
3083 209 181
BssSI C'ACGA_G
Cuts at: 1744 2102 1744
Size: 358 3115
BstAPI GCAn_nnn'nTGC
Cuts at: 850 850
Size: 3473
BstBI TT'CG_AA
Cuts at: 1834 1834
Size: 3473
BstYI r'GATC_y
Cuts at: 1 375 498 965 988 1323 1569 2581 2667
Size: 374 123 467 23 335 246 1012 86
Cuts at: 2667 2679 3025 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 374 346 335 246 123
86 23 12
BtgI C'CryG_G
Cuts at: 413 413
Size: 3473
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1423 1604 1767 1423
Size: 181 163 3129
Fragments arranged by size:
3129 181 163
BtsI GCAGTG_nn'
Cuts at: 716 716
Size: 3473
DraI TTT'AAA
Cuts at: 472 472
Size: 3473
DrdI GACnn_nn'nnGTC
Cuts at: 355 1179 2037 355
Size: 824 858 1791
Fragments arranged by size:
1791 858 824
EaeI y'GGCC_r
Cuts at: 1058 1232 1623 1650 2995 1058
Size: 174 391 27 1345 1536
Fragments arranged by size:
1536 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1058 1058
Size: 3473
EarI CTCTTCn'nnn_
Cuts at: 1496 1706 1496
Size: 210 3263
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1681 1991 2137 3283 1681
Size: 310 146 1146 1871
Fragments arranged by size:
1871 1146 310 146
EcoICRI GAG'CTC
Cuts at: 485 485
Size: 3473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 1298 1730 2477 2745 244
Size: 1054 432 747 268 972
Fragments arranged by size:
1054 972 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 276 612 276
Size: 336 3137
EcoRI G'AATT_C
Cuts at: 477 477
Size: 3473
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1492 1492
Size: 3473
FspI TGC'GCA
Cuts at: 1254 1254
Size: 3473
HaeII r_GCGC'y
Cuts at: 907 1155 2177 3156 907
Size: 248 1022 979 1224
Fragments arranged by size:
1224 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1315 1345 1347 1377 1315
Size: 30 2 30 3411
Fragments arranged by size:
3411 30 30 2
HincII GTy'rAC
Cuts at: 445 512 445
Size: 67 3406
KasI G'GCGC_C
Cuts at: 903 1151 903
Size: 248 3225
KpnI G_GTAC'C
Cuts at: 493 493
Size: 3473
MluI A'CGCG_T
Cuts at: 2906 2906
Size: 3473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2328 2144
Size: 184 3289
MscI TGG'CCA
Cuts at: 1234 1234
Size: 3473
MslI CAynn'nnrTG
Cuts at: 1462 1589 1462
Size: 127 3346
MspA1I CmG'CkG
Cuts at: 584 898 1258 2271 2516 584
Size: 314 360 1013 245 1541
Fragments arranged by size:
1541 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1654 1654
Size: 3473
NarI GG'CG_CC
Cuts at: 904 1152 904
Size: 248 3225
NgoMIV G'CCGG_C
Cuts at: 1652 1652
Size: 3473
NspI r_CATG'y
Cuts at: 526 1026 1933 526
Size: 500 907 2066
Fragments arranged by size:
2066 907 500
PciI A'CATG_T
Cuts at: 1929 1929
Size: 3473
PmeI GTTT'AAAC
Cuts at: 472 472
Size: 3473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2612 2644 2612
Size: 32 3441
PstI C_TGCA'G
Cuts at: 520 1205 520
Size: 685 2788
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3473
PvuII CAG'CTG
Cuts at: 898 1258 898
Size: 360 3113
RsrII CG'GwC_CG
Cuts at: 1668 1668
Size: 3473
SacI G_AGCT'C
Cuts at: 487 487
Size: 3473
SalI G'TCGA_C
Cuts at: 510 510
Size: 3473
SapI GCTCTTCn'nnn_
Cuts at: 1496 1706 1496
Size: 210 3263
SbfI CC_TGCA'GG
Cuts at: 520 520
Size: 3473
ScaI AGT'ACT
Cuts at: 2990 2990
Size: 3473
SfcI C'TryA_G
Cuts at: 33 516 1201 2194 2385 33
Size: 483 685 993 191 1121
Fragments arranged by size:
1121 993 685 483 191
SfoI GGC'GCC
Cuts at: 905 1153 905
Size: 248 3225
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3473
SmaI CCC'GGG
Cuts at: 495 495
Size: 3473
SmlI C'TyrA_G
Cuts at: 628 1543 2035 2297 628
Size: 915 492 262 1804
Fragments arranged by size:
1804 915 492 262
SpeI A'CTAG_T
Cuts at: 67 255 67
Size: 188 3285
SphI G_CATG'C
Cuts at: 526 1026 526
Size: 500 2973
StyI C'CwwG_G
Cuts at: 247 607 961 247
Size: 360 354 2759
Fragments arranged by size:
2759 360 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 186 1056 1189 1648 1857 186
Size: 870 133 459 209 1802
Fragments arranged by size:
1802 870 459 209 133
TatI w'GTAC_w
Cuts at: 365 2988 365
Size: 2623 850
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 3441
Tth111I GACn'n_nGTC
Cuts at: 355 1270 355
Size: 915 2558
XbaI T'CTAG_A
Cuts at: 504 504
Size: 3473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 968 968
Size: 3473
XmaI C'CCGG_G
Cuts at: 493 493
Size: 3473
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI