Restriction map for
pF1KB6785
(Circular) (Six-base) MAPSORT of: pF1KB6785.seq Check: 1384 from: 1 to: 3488
pF1KB6785 3488 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 526 526
Size: 3488
Acc65I G'GTAC_C
Cuts at: 504 504
Size: 3488
AclI AA'CG_TT
Cuts at: 3328 3328
Size: 3488
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1313 1745 2492 2760 1313
Size: 432 747 268 2041
Fragments arranged by size:
2041 747 432 268
AfeI AGC'GCT
Cuts at: 323 323
Size: 3488
AflIII A'CryG_T
Cuts at: 840 1944 2921 840
Size: 1104 977 1407
Fragments arranged by size:
1407 1104 977
AgeI A'CCGG_T
Cuts at: 1876 1876
Size: 3488
AlwNI CAG_nnn'CTG
Cuts at: 793 2360 793
Size: 1567 1921
ApaI G_GGCC'C
Cuts at: 272 272
Size: 3488
ApaLI G'TGCA_C
Cuts at: 2258 2258
Size: 3488
ApoI r'AATT_y
Cuts at: 492 2841 3097 492
Size: 2349 256 883
Fragments arranged by size:
2349 883 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3488
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3488
AvaI C'yCGr_G
Cuts at: 244 508 244
Size: 264 3224
BamHI G'GATC_C
Cuts at: 513 513
Size: 3488
BanI G'GyrC_C
Cuts at: 310 504 918 1166 1201 310
Size: 194 414 248 35 2597
Fragments arranged by size:
2597 414 248 194 35
BanII G_rGCy'C
Cuts at: 272 502 1532 272
Size: 230 1030 2228
Fragments arranged by size:
2228 1030 230
BbeI G_GCGC'C
Cuts at: 922 1170 922
Size: 248 3240
BciVI GTATCCnnnnn_n'
Cuts at: 1386 2147 3488 1386
Size: 761 1341 1386
Fragments arranged by size:
1386 1341 761
BclI T'GATC_A
Cuts at: 1008 1008
Size: 3488
BglI GCCn_nnn'nGGC
Cuts at: 248 368 431 248
Size: 120 63 3305
Fragments arranged by size:
3305 120 63
BglII A'GATC_T
Cuts at: 1003 3040 1003
Size: 2037 1451
BlpI GC'TnA_GC
Cuts at: 600 600
Size: 3488
Bme1580I G_kGCm'C
Cuts at: 272 313 1113 1206 2262 272
Size: 41 800 93 1056 1498
Fragments arranged by size:
1498 1056 800 93 41
BmgBI CAC'GTC
Cuts at: 219 219
Size: 3488
BmrI ACTGGGnnnn_n'
Cuts at: 700 781 1116 3260 700
Size: 81 335 2144 928
Fragments arranged by size:
2144 928 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1543 2035 2333 664
Size: 879 492 298 1819
Fragments arranged by size:
1819 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3192 3192
Size: 3488
BsaAI yAC'GTr
Cuts at: 841 841
Size: 3488
BsaBI GATnn'nnATC
Cuts at: 6 1002 3039 6
Size: 996 2037 455
Fragments arranged by size:
2037 996 455
BsaHI Gr'CG_yC
Cuts at: 919 1167 1869 3365 919
Size: 248 702 1496 1042
Fragments arranged by size:
1496 1042 702 248
BsaWI w'CCGG_w
Cuts at: 677 1198 1876 2150 2297 2708 677
Size: 521 678 274 147 411 1457
Fragments arranged by size:
1457 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 352
Size: 3488
BseYI C'CCAG_C
Cuts at: 118 294 476 913 1671 2248 2744 118
Size: 176 182 437 758 577 496 862
Fragments arranged by size:
862 758 577 496 437 182 176
BsiEI CG_ry'CG
Cuts at: 104 1030 1076 2284 104
Size: 926 46 1208 1308
Fragments arranged by size:
1308 1208 926 46
BsiHKAI G_wGCw'C
Cuts at: 502 1280 1470 2262 502
Size: 778 190 792 1728
Fragments arranged by size:
1728 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 272 313 502 1113 1206 1280 1470 1532 2262
Size: 41 189 611 93 74 190 62 730
Cuts at: 2262 272
Size: 1498
Fragments arranged by size:
1498 730 611 190 189 93 74 62
41
BspEI T'CCGG_A
Cuts at: 2708 2708
Size: 3488
BspHI T'CATG_A
Cuts at: 2664 2664
Size: 3488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 356 538 752 1054 1435 356
Size: 182 214 302 381 2409
Fragments arranged by size:
2409 381 302 214 182
BsrBI CCG'CTC
Cuts at: 50 389 1780 1834 3318 3483 50
Size: 339 1391 54 1484 165 55
Fragments arranged by size:
1484 1391 339 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1400 1400
Size: 3488
BsrFI r'CCGG_y
Cuts at: 112 1486 1667 1876 112
Size: 1374 181 209 1724
Fragments arranged by size:
1724 1374 209 181
BssSI C'ACGA_G
Cuts at: 1759 2117 1759
Size: 358 3130
BstAPI GCAn_nnn'nTGC
Cuts at: 865 865
Size: 3488
BstBI TT'CG_AA
Cuts at: 1849 1849
Size: 3488
BstYI r'GATC_y
Cuts at: 1 513 980 1003 1338 1584 2596 2682 2694
Size: 512 467 23 335 246 1012 86 12
Cuts at: 2694 3040 1
Size: 346 449
Fragments arranged by size:
1012 512 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3488
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1438 1619 1782 1438
Size: 181 163 3144
Fragments arranged by size:
3144 181 163
BtsI GCAGTG_nn'
Cuts at: 731 731
Size: 3488
DraI TTT'AAA
Cuts at: 487 487
Size: 3488
DrdI GACnn_nn'nnGTC
Cuts at: 1194 2052 1194
Size: 858 2630
EaeI y'GGCC_r
Cuts at: 110 369 1073 1247 1638 1665 3010 110
Size: 259 704 174 391 27 1345 588
Fragments arranged by size:
1345 704 588 391 259 174 27
EagI C'GGCC_G
Cuts at: 1073 1073
Size: 3488
EarI CTCTTCn'nnn_
Cuts at: 284 1511 1721 284
Size: 1227 210 2051
Fragments arranged by size:
2051 1227 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 150 1696 2006 2152 3298 150
Size: 1546 310 146 1146 340
Fragments arranged by size:
1546 1146 340 310 146
EcoICRI GAG'CTC
Cuts at: 500 500
Size: 3488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1313 1745 2492 2760 1313
Size: 432 747 268 2041
Fragments arranged by size:
2041 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 627 627
Size: 3488
EcoRI G'AATT_C
Cuts at: 492 492
Size: 3488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1507 1507
Size: 3488
FseI GG_CCGG'CC
Cuts at: 116 116
Size: 3488
FspI TGC'GCA
Cuts at: 223 1269 223
Size: 1046 2442
HaeII r_GCGC'y
Cuts at: 154 325 922 1170 2192 3171 154
Size: 171 597 248 1022 979 471
Fragments arranged by size:
1022 979 597 471 248 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1330 1360 1362 1392 1330
Size: 30 2 30 3426
Fragments arranged by size:
3426 30 30 2
HincII GTy'rAC
Cuts at: 527 527
Size: 3488
KasI G'GCGC_C
Cuts at: 918 1166 918
Size: 248 3240
KpnI G_GTAC'C
Cuts at: 508 508
Size: 3488
MluI A'CGCG_T
Cuts at: 2921 2921
Size: 3488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2343 2159
Size: 184 3304
MscI TGG'CCA
Cuts at: 1249 1249
Size: 3488
MslI CAynn'nnrTG
Cuts at: 1477 1604 1477
Size: 127 3361
MspA1I CmG'CkG
Cuts at: 260 398 599 913 1273 2286 2531 260
Size: 138 201 314 360 1013 245 1217
Fragments arranged by size:
1217 1013 360 314 245 201 138
NaeI GCC'GGC
Cuts at: 114 1669 114
Size: 1555 1933
NarI GG'CG_CC
Cuts at: 919 1167 919
Size: 248 3240
NcoI C'CATG_G
Cuts at: 107 107
Size: 3488
NgoMIV G'CCGG_C
Cuts at: 112 1667 112
Size: 1555 1933
NspI r_CATG'y
Cuts at: 541 1041 1948 541
Size: 500 907 2081
Fragments arranged by size:
2081 907 500
PasI CC'CwG_GG
Cuts at: 186 186
Size: 3488
PciI A'CATG_T
Cuts at: 1944 1944
Size: 3488
PmeI GTTT'AAAC
Cuts at: 487 487
Size: 3488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 3456
PspOMI G'GGCC_C
Cuts at: 268 268
Size: 3488
PstI C_TGCA'G
Cuts at: 424 535 1220 424
Size: 111 685 2692
Fragments arranged by size:
2692 685 111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3488
PvuII CAG'CTG
Cuts at: 913 1273 913
Size: 360 3128
RsrII CG'GwC_CG
Cuts at: 1683 1683
Size: 3488
SacI G_AGCT'C
Cuts at: 502 502
Size: 3488
SalI G'TCGA_C
Cuts at: 525 525
Size: 3488
SapI GCTCTTCn'nnn_
Cuts at: 284 1511 1721 284
Size: 1227 210 2051
Fragments arranged by size:
2051 1227 210
SbfI CC_TGCA'GG
Cuts at: 535 535
Size: 3488
ScaI AGT'ACT
Cuts at: 3005 3005
Size: 3488
SfcI C'TryA_G
Cuts at: 33 420 531 1216 2209 2400 33
Size: 387 111 685 993 191 1121
Fragments arranged by size:
1121 993 685 387 191 111
SfoI GGC'GCC
Cuts at: 920 1168 920
Size: 248 3240
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3488
SmaI CCC'GGG
Cuts at: 246 510 246
Size: 264 3224
SmlI C'TyrA_G
Cuts at: 643 1558 2050 2312 643
Size: 915 492 262 1819
Fragments arranged by size:
1819 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3488
SphI G_CATG'C
Cuts at: 541 1041 541
Size: 500 2988
StyI C'CwwG_G
Cuts at: 107 622 976 107
Size: 515 354 2619
Fragments arranged by size:
2619 515 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1071 1204 1663 1872 1071
Size: 133 459 209 2687
Fragments arranged by size:
2687 459 209 133
TatI w'GTAC_w
Cuts at: 3003 3003
Size: 3488
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 3456
Tth111I GACn'n_nGTC
Cuts at: 1285 1285
Size: 3488
XbaI T'CTAG_A
Cuts at: 519 519
Size: 3488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 983 983
Size: 3488
XmaI C'CCGG_G
Cuts at: 244 508 244
Size: 264 3224
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI