Restriction map for
pF1KB6788
(Circular) (Six-base) MAPSORT of: pF1KB6788.seq Check: 6979 from: 1 to: 3575
pF1KB6788 3575 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 171 171
Size: 3575
AccI GT'mk_AC
Cuts at: 613 613
Size: 3575
Acc65I G'GTAC_C
Cuts at: 591 591
Size: 3575
AclI AA'CG_TT
Cuts at: 3415 3415
Size: 3575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 415 1400 1832 2579 2847 310
Size: 105 985 432 747 268 1038
Fragments arranged by size:
1038 985 747 432 268 105
AflIII A'CryG_T
Cuts at: 927 2031 3008 927
Size: 1104 977 1494
Fragments arranged by size:
1494 1104 977
AgeI A'CCGG_T
Cuts at: 1963 1963
Size: 3575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 203 203
Size: 3575
AlwNI CAG_nnn'CTG
Cuts at: 880 2447 880
Size: 1567 2008
ApaLI G'TGCA_C
Cuts at: 2345 2345
Size: 3575
ApoI r'AATT_y
Cuts at: 457 579 2928 3184 457
Size: 122 2349 256 848
Fragments arranged by size:
2349 848 256 122
AseI AT'TA_AT
Cuts at: 20 20
Size: 3575
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
AvaI C'yCGr_G
Cuts at: 595 595
Size: 3575
BamHI G'GATC_C
Cuts at: 600 600
Size: 3575
BanI G'GyrC_C
Cuts at: 591 1005 1253 1288 591
Size: 414 248 35 2878
Fragments arranged by size:
2878 414 248 35
BanII G_rGCy'C
Cuts at: 589 1619 589
Size: 1030 2545
BbeI G_GCGC'C
Cuts at: 1009 1257 1009
Size: 248 3327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 460 494 460
Size: 34 3541
BciVI GTATCCnnnnn_n'
Cuts at: 1473 2234 3575 1473
Size: 761 1341 1473
Fragments arranged by size:
1473 1341 761
BclI T'GATC_A
Cuts at: 1095 1095
Size: 3575
BglII A'GATC_T
Cuts at: 1090 3127 1090
Size: 2037 1538
BlpI GC'TnA_GC
Cuts at: 549 687 549
Size: 138 3437
Bme1580I G_kGCm'C
Cuts at: 1200 1293 2349 1200
Size: 93 1056 2426
Fragments arranged by size:
2426 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 787 868 1203 3347 787
Size: 81 335 2144 1015
Fragments arranged by size:
2144 1015 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 575
Size: 3575
Bpu10I CC'TnA_GC
Cuts at: 539 539
Size: 3575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1630 2122 2420 751
Size: 879 492 298 1906
Fragments arranged by size:
1906 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 570 3279 570
Size: 2709 866
BsaAI yAC'GTr
Cuts at: 928 928
Size: 3575
BsaBI GATnn'nnATC
Cuts at: 6 1089 3126 6
Size: 1083 2037 455
Fragments arranged by size:
2037 1083 455
BsaHI Gr'CG_yC
Cuts at: 1006 1254 1956 3452 1006
Size: 248 702 1496 1129
Fragments arranged by size:
1496 1129 702 248
BsaWI w'CCGG_w
Cuts at: 764 1285 1963 2237 2384 2795 764
Size: 521 678 274 147 411 1544
Fragments arranged by size:
1544 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 241 271 326 356 548 578 241
Size: 30 55 30 192 30 3238
Fragments arranged by size:
3238 192 55 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 347 347
Size: 3575
BseYI C'CCAG_C
Cuts at: 532 1000 1758 2335 2831 532
Size: 468 758 577 496 1276
Fragments arranged by size:
1276 758 577 496 468
BsiEI CG_ry'CG
Cuts at: 104 126 1117 1163 2371 104
Size: 22 991 46 1208 1308
Fragments arranged by size:
1308 1208 991 46 22
BsiHKAI G_wGCw'C
Cuts at: 269 589 1367 1557 2349 269
Size: 320 778 190 792 1495
Fragments arranged by size:
1495 792 778 320 190
BsmBI CGTCTCn'nnnn_
Cuts at: 487 487
Size: 3575
Bsp1286I G_dGCh'C
Cuts at: 269 589 1200 1293 1367 1557 1619 2349 269
Size: 320 611 93 74 190 62 730 1495
Fragments arranged by size:
1495 730 611 320 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2795 2795
Size: 3575
BspHI T'CATG_A
Cuts at: 2751 2751
Size: 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 171 625 839 1141 1522 171
Size: 454 214 302 381 2224
Fragments arranged by size:
2224 454 381 302 214
BsrBI CCG'CTC
Cuts at: 50 149 1867 1921 3405 3570 50
Size: 99 1718 54 1484 165 55
Fragments arranged by size:
1718 1484 165 99 55 54
BsrDI GCAATG_nn'
Cuts at: 1487 1487
Size: 3575
BsrFI r'CCGG_y
Cuts at: 130 1573 1754 1963 130
Size: 1443 181 209 1742
Fragments arranged by size:
1742 1443 209 181
BssSI C'ACGA_G
Cuts at: 1846 2204 1846
Size: 358 3217
BstAPI GCAn_nnn'nTGC
Cuts at: 952 952
Size: 3575
BstBI TT'CG_AA
Cuts at: 1936 1936
Size: 3575
BstYI r'GATC_y
Cuts at: 1 600 1067 1090 1425 1671 2683 2769 2781
Size: 599 467 23 335 246 1012 86 12
Cuts at: 2781 3127 1
Size: 346 449
Fragments arranged by size:
1012 599 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1525 1706 1869 1525
Size: 181 163 3231
Fragments arranged by size:
3231 181 163
BtsI GCAGTG_nn'
Cuts at: 818 818
Size: 3575
ClaI AT'CG_AT
Cuts at: 281 281
Size: 3575
DraI TTT'AAA
Cuts at: 574 574
Size: 3575
DrdI GACnn_nn'nnGTC
Cuts at: 1281 2139 1281
Size: 858 2717
EaeI y'GGCC_r
Cuts at: 1160 1334 1725 1752 3097 1160
Size: 174 391 27 1345 1638
Fragments arranged by size:
1638 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1160 1160
Size: 3575
EarI CTCTTCn'nnn_
Cuts at: 1598 1808 1598
Size: 210 3365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1783 2093 2239 3385 127
Size: 1656 310 146 1146 317
Fragments arranged by size:
1656 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 587 587
Size: 3575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 415 575 1400 1832 2579 2847 310
Size: 105 160 825 432 747 268 1038
Fragments arranged by size:
1038 825 747 432 268 160 105
EcoNI CCTnn'n_nnAGG
Cuts at: 208 208
Size: 3575
EcoO109I rG'GnC_Cy
Cuts at: 714 714
Size: 3575
EcoRI G'AATT_C
Cuts at: 457 579 457
Size: 122 3453
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1594 1594
Size: 3575
FspI TGC'GCA
Cuts at: 1356 1356
Size: 3575
HaeII r_GCGC'y
Cuts at: 1009 1257 2279 3258 1009
Size: 248 1022 979 1326
Fragments arranged by size:
1326 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 548 580 1417 1447 1449 1479 548
Size: 32 837 30 2 30 2644
Fragments arranged by size:
2644 837 32 30 30 2
HincII GTy'rAC
Cuts at: 614 614
Size: 3575
KasI G'GCGC_C
Cuts at: 1005 1253 1005
Size: 248 3327
KpnI G_GTAC'C
Cuts at: 595 595
Size: 3575
MluI A'CGCG_T
Cuts at: 3008 3008
Size: 3575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 309 2246 2430 227
Size: 82 1937 184 1372
Fragments arranged by size:
1937 1372 184 82
MscI TGG'CCA
Cuts at: 1336 1336
Size: 3575
MslI CAynn'nnrTG
Cuts at: 1564 1691 1564
Size: 127 3448
MspA1I CmG'CkG
Cuts at: 122 143 241 553 686 1000 1360 2373 2618
Size: 21 98 312 133 314 360 1013 245
Cuts at: 2618 122
Size: 1079
Fragments arranged by size:
1079 1013 360 314 312 245 133 98
21
NaeI GCC'GGC
Cuts at: 132 1756 132
Size: 1624 1951
NarI GG'CG_CC
Cuts at: 1006 1254 1006
Size: 248 3327
NcoI C'CATG_G
Cuts at: 107 107
Size: 3575
NgoMIV G'CCGG_C
Cuts at: 130 1754 130
Size: 1624 1951
NspI r_CATG'y
Cuts at: 628 1128 2035 628
Size: 500 907 2168
Fragments arranged by size:
2168 907 500
PciI A'CATG_T
Cuts at: 2031 2031
Size: 3575
PfoI T'CCnGG_A
Cuts at: 461 461
Size: 3575
PmeI GTTT'AAAC
Cuts at: 574 574
Size: 3575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 3543
PstI C_TGCA'G
Cuts at: 622 1307 622
Size: 685 2890
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3575
PvuII CAG'CTG
Cuts at: 241 553 1000 1360 241
Size: 312 447 360 2456
Fragments arranged by size:
2456 447 360 312
RsrII CG'GwC_CG
Cuts at: 1770 1770
Size: 3575
SacI G_AGCT'C
Cuts at: 589 589
Size: 3575
SalI G'TCGA_C
Cuts at: 612 612
Size: 3575
SapI GCTCTTCn'nnn_
Cuts at: 1598 1808 1598
Size: 210 3365
SbfI CC_TGCA'GG
Cuts at: 622 622
Size: 3575
ScaI AGT'ACT
Cuts at: 3092 3092
Size: 3575
SfcI C'TryA_G
Cuts at: 33 618 1303 2296 2487 33
Size: 585 685 993 191 1121
Fragments arranged by size:
1121 993 685 585 191
SfoI GGC'GCC
Cuts at: 1007 1255 1007
Size: 248 3327
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
SmaI CCC'GGG
Cuts at: 597 597
Size: 3575
SmlI C'TyrA_G
Cuts at: 730 1645 2137 2399 730
Size: 915 492 262 1906
Fragments arranged by size:
1906 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3575
SphI G_CATG'C
Cuts at: 628 1128 628
Size: 500 3075
StuI AGG'CCT
Cuts at: 224 224
Size: 3575
StyI C'CwwG_G
Cuts at: 107 512 709 1063 107
Size: 405 197 354 2619
Fragments arranged by size:
2619 405 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1158 1291 1750 1959 1158
Size: 133 459 209 2774
Fragments arranged by size:
2774 459 209 133
TatI w'GTAC_w
Cuts at: 3090 3090
Size: 3575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 326 358 3092 3124 326
Size: 32 2734 32 777
Fragments arranged by size:
2734 777 32 32
Tth111I GACn'n_nGTC
Cuts at: 1372 1372
Size: 3575
XbaI T'CTAG_A
Cuts at: 606 606
Size: 3575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1070 1070
Size: 3575
XmaI C'CCGG_G
Cuts at: 595 595
Size: 3575
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI