(Circular) (Six-base) MAPSORT of: pF1KB6789.seq Check: 3081 from: 1 to: 3488 pF1KB6789 3488 bp With 182 enzymes: SgfI * October 7, 2009 14:22 .. AccI GT'mk_AC Cuts at: 452 526 452 Size: 74 3414 Acc65I G'GTAC_C Cuts at: 504 504 Size: 3488 AclI AA'CG_TT Cuts at: 3328 3328 Size: 3488 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1313 1745 2492 2760 1313 Size: 432 747 268 2041 Fragments arranged by size: 2041 747 432 268 AflIII A'CryG_T Cuts at: 840 1944 2921 840 Size: 1104 977 1407 Fragments arranged by size: 1407 1104 977 AgeI A'CCGG_T Cuts at: 1876 1876 Size: 3488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 178 219 178 Size: 41 3447 AlwNI CAG_nnn'CTG Cuts at: 793 2360 793 Size: 1567 1921 ApaLI G'TGCA_C Cuts at: 2258 2258 Size: 3488 ApoI r'AATT_y Cuts at: 492 2841 3097 492 Size: 2349 256 883 Fragments arranged by size: 2349 883 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3488 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3488 AvaI C'yCGr_G Cuts at: 508 508 Size: 3488 BamHI G'GATC_C Cuts at: 513 513 Size: 3488 BanI G'GyrC_C Cuts at: 504 918 1166 1201 504 Size: 414 248 35 2791 Fragments arranged by size: 2791 414 248 35 BanII G_rGCy'C Cuts at: 402 502 1532 402 Size: 100 1030 2358 Fragments arranged by size: 2358 1030 100 BbeI G_GCGC'C Cuts at: 922 1170 922 Size: 248 3240 BbsI GAAGACnn'nnnn_ Cuts at: 295 295 Size: 3488 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3454 BciVI GTATCCnnnnn_n' Cuts at: 1386 2147 3488 1386 Size: 761 1341 1386 Fragments arranged by size: 1386 1341 761 BclI T'GATC_A Cuts at: 1008 1008 Size: 3488 BglII A'GATC_T Cuts at: 1003 3040 1003 Size: 2037 1451 BlpI GC'TnA_GC Cuts at: 600 600 Size: 3488 Bme1580I G_kGCm'C Cuts at: 1113 1206 2262 1113 Size: 93 1056 2339 Fragments arranged by size: 2339 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 405 700 781 1116 3260 405 Size: 295 81 335 2144 633 Fragments arranged by size: 2144 633 335 295 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1855 1855 Size: 3488 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 355 664 1543 2035 2333 355 Size: 309 879 492 298 1510 Fragments arranged by size: 1510 879 492 309 298 BsaI GGTCTCn'nnnn_ Cuts at: 3192 3192 Size: 3488 BsaAI yAC'GTr Cuts at: 841 841 Size: 3488 BsaBI GATnn'nnATC Cuts at: 6 1002 3039 6 Size: 996 2037 455 Fragments arranged by size: 2037 996 455 BsaHI Gr'CG_yC Cuts at: 919 1167 1869 3365 919 Size: 248 702 1496 1042 Fragments arranged by size: 1496 1042 702 248 BsaWI w'CCGG_w Cuts at: 677 1198 1876 2150 2297 2708 677 Size: 521 678 274 147 411 1457 Fragments arranged by size: 1457 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 189 189 Size: 3488 BseYI C'CCAG_C Cuts at: 913 1671 2248 2744 913 Size: 758 577 496 1657 Fragments arranged by size: 1657 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 422 422 Size: 3488 BsiEI CG_ry'CG Cuts at: 104 1030 1076 2284 104 Size: 926 46 1208 1308 Fragments arranged by size: 1308 1208 926 46 BsiHKAI G_wGCw'C Cuts at: 502 1280 1470 2262 502 Size: 778 190 792 1728 Fragments arranged by size: 1728 792 778 190 Bsp1286I G_dGCh'C Cuts at: 402 502 1113 1206 1280 1470 1532 2262 402 Size: 100 611 93 74 190 62 730 1628 Fragments arranged by size: 1628 730 611 190 100 93 74 62 BspEI T'CCGG_A Cuts at: 2708 2708 Size: 3488 BspHI T'CATG_A Cuts at: 2664 2664 Size: 3488 BspMI ACCTGCnnnn'nnnn_ Cuts at: 211 538 752 1054 1435 211 Size: 327 214 302 381 2264 Fragments arranged by size: 2264 381 327 302 214 BsrBI CCG'CTC Cuts at: 50 1780 1834 3318 3483 50 Size: 1730 54 1484 165 55 Fragments arranged by size: 1730 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 428 1400 428 Size: 972 2516 BsrFI r'CCGG_y Cuts at: 1486 1667 1876 1486 Size: 181 209 3098 Fragments arranged by size: 3098 209 181 BssSI C'ACGA_G Cuts at: 1759 2117 1759 Size: 358 3130 BstAPI GCAn_nnn'nTGC Cuts at: 865 865 Size: 3488 BstBI TT'CG_AA Cuts at: 1849 1849 Size: 3488 BstXI CCAn_nnnn'nTGG Cuts at: 441 441 Size: 3488 BstYI r'GATC_y Cuts at: 1 513 980 1003 1338 1584 2596 2682 2694 Size: 512 467 23 335 246 1012 86 12 Cuts at: 2694 3040 1 Size: 346 449 Fragments arranged by size: 1012 512 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1438 1619 1782 1438 Size: 181 163 3144 Fragments arranged by size: 3144 181 163 BtsI GCAGTG_nn' Cuts at: 191 434 731 191 Size: 243 297 2948 Fragments arranged by size: 2948 297 243 DraI TTT'AAA Cuts at: 487 487 Size: 3488 DrdI GACnn_nn'nnGTC Cuts at: 1194 2052 1194 Size: 858 2630 EaeI y'GGCC_r Cuts at: 1073 1247 1638 1665 3010 1073 Size: 174 391 27 1345 1551 Fragments arranged by size: 1551 1345 391 174 27 EagI C'GGCC_G Cuts at: 1073 1073 Size: 3488 EarI CTCTTCn'nnn_ Cuts at: 1511 1721 1511 Size: 210 3278 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1696 2006 2152 3298 1696 Size: 310 146 1146 1886 Fragments arranged by size: 1886 1146 310 146 EcoICRI GAG'CTC Cuts at: 500 500 Size: 3488 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1313 1745 2492 2760 1313 Size: 432 747 268 2041 Fragments arranged by size: 2041 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 627 627 Size: 3488 EcoRI G'AATT_C Cuts at: 492 492 Size: 3488 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1507 1507 Size: 3488 FspI TGC'GCA Cuts at: 424 1269 424 Size: 845 2643 FspAI rTGC'GCAy Cuts at: 424 424 Size: 3488 HaeII r_GCGC'y Cuts at: 922 1170 2192 3171 922 Size: 248 1022 979 1239 Fragments arranged by size: 1239 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 173 205 1330 1360 1362 1392 173 Size: 32 1125 30 2 30 2269 Fragments arranged by size: 2269 1125 32 30 30 2 HincII GTy'rAC Cuts at: 527 527 Size: 3488 HindIII A'AGCT_T Cuts at: 215 215 Size: 3488 KasI G'GCGC_C Cuts at: 918 1166 918 Size: 248 3240 KpnI G_GTAC'C Cuts at: 508 508 Size: 3488 MluI A'CGCG_T Cuts at: 2921 2921 Size: 3488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2159 2343 2159 Size: 184 3304 MscI TGG'CCA Cuts at: 1249 1249 Size: 3488 MslI CAynn'nnrTG Cuts at: 233 1477 1604 233 Size: 1244 127 2117 Fragments arranged by size: 2117 1244 127 MspA1I CmG'CkG Cuts at: 465 599 913 1273 2286 2531 465 Size: 134 314 360 1013 245 1422 Fragments arranged by size: 1422 1013 360 314 245 134 NaeI GCC'GGC Cuts at: 1669 1669 Size: 3488 NarI GG'CG_CC Cuts at: 919 1167 919 Size: 248 3240 NgoMIV G'CCGG_C Cuts at: 1667 1667 Size: 3488 NspI r_CATG'y Cuts at: 423 541 1041 1948 423 Size: 118 500 907 1963 Fragments arranged by size: 1963 907 500 118 PciI A'CATG_T Cuts at: 1944 1944 Size: 3488 PmeI GTTT'AAAC Cuts at: 487 487 Size: 3488 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2627 2659 2627 Size: 32 3456 PstI C_TGCA'G Cuts at: 167 198 470 535 1220 167 Size: 31 272 65 685 2435 Fragments arranged by size: 2435 685 272 65 31 PvuI CG_AT'CG Cuts at: 104 104 Size: 3488 PvuII CAG'CTG Cuts at: 465 913 1273 465 Size: 448 360 2680 Fragments arranged by size: 2680 448 360 RsrII CG'GwC_CG Cuts at: 1683 1683 Size: 3488 SacI G_AGCT'C Cuts at: 502 502 Size: 3488 SalI G'TCGA_C Cuts at: 525 525 Size: 3488 SapI GCTCTTCn'nnn_ Cuts at: 1511 1721 1511 Size: 210 3278 SbfI CC_TGCA'GG Cuts at: 535 535 Size: 3488 ScaI AGT'ACT Cuts at: 3005 3005 Size: 3488 SfcI C'TryA_G Cuts at: 33 163 194 295 466 531 1216 2209 2400 Size: 130 31 101 171 65 685 993 191 Cuts at: 2400 33 Size: 1121 Fragments arranged by size: 1121 993 685 191 171 130 101 65 31 SfoI GGC'GCC Cuts at: 920 1168 920 Size: 248 3240 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3488 SmaI CCC'GGG Cuts at: 510 510 Size: 3488 SmlI C'TyrA_G Cuts at: 370 643 1558 2050 2312 370 Size: 273 915 492 262 1546 Fragments arranged by size: 1546 915 492 273 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3488 SphI G_CATG'C Cuts at: 541 1041 541 Size: 500 2988 StuI AGG'CCT Cuts at: 253 253 Size: 3488 StyI C'CwwG_G Cuts at: 622 976 622 Size: 354 3134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 229 1071 1204 1663 1872 229 Size: 842 133 459 209 1845 Fragments arranged by size: 1845 842 459 209 133 TatI w'GTAC_w Cuts at: 3003 3003 Size: 3488 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3005 3037 3005 Size: 32 3456 Tth111I GACn'n_nGTC Cuts at: 1285 1285 Size: 3488 XbaI T'CTAG_A Cuts at: 519 519 Size: 3488 XcmI CCAnnnn_n'nnnnTGG Cuts at: 983 983 Size: 3488 XmaI C'CCGG_G Cuts at: 508 508 Size: 3488 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI