Restriction map for pF1KB6789
 (Circular) (Six-base) MAPSORT of: pF1KB6789.seq  Check: 3081  from: 1  to: 3488



pF1KB6789  3488 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AccI GT'mk_AC

Cuts at:    452     526     452 
   Size:         74    3414
 
Acc65I G'GTAC_C

Cuts at:    504     504 
   Size:       3488
 
AclI AA'CG_TT

Cuts at:   3328    3328 
   Size:       3488
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1313    1745    2492    2760    1313 
   Size:        432     747     268    2041

  Fragments arranged by size: 

                2041     747     432     268
 
AflIII A'CryG_T

Cuts at:    840    1944    2921     840 
   Size:       1104     977    1407

  Fragments arranged by size: 

                1407    1104     977
 
AgeI A'CCGG_T

Cuts at:   1876    1876 
   Size:       3488
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    178     219     178 
   Size:         41    3447
 
AlwNI CAG_nnn'CTG

Cuts at:    793    2360     793 
   Size:       1567    1921
 
ApaLI G'TGCA_C

Cuts at:   2258    2258 
   Size:       3488
 
ApoI r'AATT_y

Cuts at:    492    2841    3097     492 
   Size:       2349     256     883

  Fragments arranged by size: 

                2349     883     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3488
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3488
 
AvaI C'yCGr_G

Cuts at:    508     508 
   Size:       3488
 
BamHI G'GATC_C

Cuts at:    513     513 
   Size:       3488
 
BanI G'GyrC_C

Cuts at:    504     918    1166    1201     504 
   Size:        414     248      35    2791

  Fragments arranged by size: 

                2791     414     248      35
 
BanII G_rGCy'C

Cuts at:    402     502    1532     402 
   Size:        100    1030    2358

  Fragments arranged by size: 

                2358    1030     100
 
BbeI G_GCGC'C

Cuts at:    922    1170     922 
   Size:        248    3240
 
BbsI GAAGACnn'nnnn_

Cuts at:    295     295 
   Size:       3488
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3454
 
BciVI GTATCCnnnnn_n'

Cuts at:   1386    2147    3488    1386 
   Size:        761    1341    1386

  Fragments arranged by size: 

                1386    1341     761
 
BclI T'GATC_A

Cuts at:   1008    1008 
   Size:       3488
 
BglII A'GATC_T

Cuts at:   1003    3040    1003 
   Size:       2037    1451
 
BlpI GC'TnA_GC

Cuts at:    600     600 
   Size:       3488
 
Bme1580I G_kGCm'C

Cuts at:   1113    1206    2262    1113 
   Size:         93    1056    2339

  Fragments arranged by size: 

                2339    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    405     700     781    1116    3260     405 
   Size:        295      81     335    2144     633

  Fragments arranged by size: 

                2144     633     335     295      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1855    1855 
   Size:       3488
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    355     664    1543    2035    2333     355 
   Size:        309     879     492     298    1510

  Fragments arranged by size: 

                1510     879     492     309     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3192    3192 
   Size:       3488
 
BsaAI yAC'GTr

Cuts at:    841     841 
   Size:       3488
 
BsaBI GATnn'nnATC

Cuts at:      6    1002    3039       6 
   Size:        996    2037     455

  Fragments arranged by size: 

                2037     996     455
 
BsaHI Gr'CG_yC

Cuts at:    919    1167    1869    3365     919 
   Size:        248     702    1496    1042

  Fragments arranged by size: 

                1496    1042     702     248
 
BsaWI w'CCGG_w

Cuts at:    677    1198    1876    2150    2297    2708     677 
   Size:        521     678     274     147     411    1457

  Fragments arranged by size: 

                1457     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    189     189 
   Size:       3488
 
BseYI C'CCAG_C

Cuts at:    913    1671    2248    2744     913 
   Size:        758     577     496    1657

  Fragments arranged by size: 

                1657     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    422     422 
   Size:       3488
 
BsiEI CG_ry'CG

Cuts at:    104    1030    1076    2284     104 
   Size:        926      46    1208    1308

  Fragments arranged by size: 

                1308    1208     926      46
 
BsiHKAI G_wGCw'C

Cuts at:    502    1280    1470    2262     502 
   Size:        778     190     792    1728

  Fragments arranged by size: 

                1728     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    402     502    1113    1206    1280    1470    1532    2262     402 
   Size:        100     611      93      74     190      62     730    1628

  Fragments arranged by size: 

                1628     730     611     190     100      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2708    2708 
   Size:       3488
 
BspHI T'CATG_A

Cuts at:   2664    2664 
   Size:       3488
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    211     538     752    1054    1435     211 
   Size:        327     214     302     381    2264

  Fragments arranged by size: 

                2264     381     327     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1780    1834    3318    3483      50 
   Size:       1730      54    1484     165      55

  Fragments arranged by size: 

                1730    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    428    1400     428 
   Size:        972    2516
 
BsrFI r'CCGG_y

Cuts at:   1486    1667    1876    1486 
   Size:        181     209    3098

  Fragments arranged by size: 

                3098     209     181
 
BssSI C'ACGA_G

Cuts at:   1759    2117    1759 
   Size:        358    3130
 
BstAPI GCAn_nnn'nTGC

Cuts at:    865     865 
   Size:       3488
 
BstBI TT'CG_AA

Cuts at:   1849    1849 
   Size:       3488
 
BstXI CCAn_nnnn'nTGG

Cuts at:    441     441 
   Size:       3488
 
BstYI r'GATC_y

Cuts at:      1     513     980    1003    1338    1584    2596    2682    2694 
   Size:        512     467      23     335     246    1012      86      12

Cuts at:   2694    3040       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     512     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1438    1619    1782    1438 
   Size:        181     163    3144

  Fragments arranged by size: 

                3144     181     163
 
BtsI GCAGTG_nn'

Cuts at:    191     434     731     191 
   Size:        243     297    2948

  Fragments arranged by size: 

                2948     297     243
 
DraI TTT'AAA

Cuts at:    487     487 
   Size:       3488
 
DrdI GACnn_nn'nnGTC

Cuts at:   1194    2052    1194 
   Size:        858    2630
 
EaeI y'GGCC_r

Cuts at:   1073    1247    1638    1665    3010    1073 
   Size:        174     391      27    1345    1551

  Fragments arranged by size: 

                1551    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1073    1073 
   Size:       3488
 
EarI CTCTTCn'nnn_

Cuts at:   1511    1721    1511 
   Size:        210    3278
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1696    2006    2152    3298    1696 
   Size:        310     146    1146    1886

  Fragments arranged by size: 

                1886    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    500     500 
   Size:       3488
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1313    1745    2492    2760    1313 
   Size:        432     747     268    2041

  Fragments arranged by size: 

                2041     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    627     627 
   Size:       3488
 
EcoRI G'AATT_C

Cuts at:    492     492 
   Size:       3488
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1507    1507 
   Size:       3488
 
FspI TGC'GCA

Cuts at:    424    1269     424 
   Size:        845    2643
 
FspAI rTGC'GCAy

Cuts at:    424     424 
   Size:       3488
 
HaeII r_GCGC'y

Cuts at:    922    1170    2192    3171     922 
   Size:        248    1022     979    1239

  Fragments arranged by size: 

                1239    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    173     205    1330    1360    1362    1392     173 
   Size:         32    1125      30       2      30    2269

  Fragments arranged by size: 

                2269    1125      32      30      30       2
 
HincII GTy'rAC

Cuts at:    527     527 
   Size:       3488
 
HindIII A'AGCT_T

Cuts at:    215     215 
   Size:       3488
 
KasI G'GCGC_C

Cuts at:    918    1166     918 
   Size:        248    3240
 
KpnI G_GTAC'C

Cuts at:    508     508 
   Size:       3488
 
MluI A'CGCG_T

Cuts at:   2921    2921 
   Size:       3488
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2159    2343    2159 
   Size:        184    3304
 
MscI TGG'CCA

Cuts at:   1249    1249 
   Size:       3488
 
MslI CAynn'nnrTG

Cuts at:    233    1477    1604     233 
   Size:       1244     127    2117

  Fragments arranged by size: 

                2117    1244     127
 
MspA1I CmG'CkG

Cuts at:    465     599     913    1273    2286    2531     465 
   Size:        134     314     360    1013     245    1422

  Fragments arranged by size: 

                1422    1013     360     314     245     134
 
NaeI GCC'GGC

Cuts at:   1669    1669 
   Size:       3488
 
NarI GG'CG_CC

Cuts at:    919    1167     919 
   Size:        248    3240
 
NgoMIV G'CCGG_C

Cuts at:   1667    1667 
   Size:       3488
 
NspI r_CATG'y

Cuts at:    423     541    1041    1948     423 
   Size:        118     500     907    1963

  Fragments arranged by size: 

                1963     907     500     118
 
PciI A'CATG_T

Cuts at:   1944    1944 
   Size:       3488
 
PmeI GTTT'AAAC

Cuts at:    487     487 
   Size:       3488
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2627    2659    2627 
   Size:         32    3456
 
PstI C_TGCA'G

Cuts at:    167     198     470     535    1220     167 
   Size:         31     272      65     685    2435

  Fragments arranged by size: 

                2435     685     272      65      31
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3488
 
PvuII CAG'CTG

Cuts at:    465     913    1273     465 
   Size:        448     360    2680

  Fragments arranged by size: 

                2680     448     360
 
RsrII CG'GwC_CG

Cuts at:   1683    1683 
   Size:       3488
 
SacI G_AGCT'C

Cuts at:    502     502 
   Size:       3488
 
SalI G'TCGA_C

Cuts at:    525     525 
   Size:       3488
 
SapI GCTCTTCn'nnn_

Cuts at:   1511    1721    1511 
   Size:        210    3278
 
SbfI CC_TGCA'GG

Cuts at:    535     535 
   Size:       3488
 
ScaI AGT'ACT

Cuts at:   3005    3005 
   Size:       3488
 
SfcI C'TryA_G

Cuts at:     33     163     194     295     466     531    1216    2209    2400 
   Size:        130      31     101     171      65     685     993     191

Cuts at:   2400      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     191     171     130     101      65
                  31
 
SfoI GGC'GCC

Cuts at:    920    1168     920 
   Size:        248    3240
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3488
 
SmaI CCC'GGG

Cuts at:    510     510 
   Size:       3488
 
SmlI C'TyrA_G

Cuts at:    370     643    1558    2050    2312     370 
   Size:        273     915     492     262    1546

  Fragments arranged by size: 

                1546     915     492     273     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3488
 
SphI G_CATG'C

Cuts at:    541    1041     541 
   Size:        500    2988
 
StuI AGG'CCT

Cuts at:    253     253 
   Size:       3488
 
StyI C'CwwG_G

Cuts at:    622     976     622 
   Size:        354    3134
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    229    1071    1204    1663    1872     229 
   Size:        842     133     459     209    1845

  Fragments arranged by size: 

                1845     842     459     209     133
 
TatI w'GTAC_w

Cuts at:   3003    3003 
   Size:       3488
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3005    3037    3005 
   Size:         32    3456
 
Tth111I GACn'n_nGTC

Cuts at:   1285    1285 
   Size:       3488
 
XbaI T'CTAG_A

Cuts at:    519     519 
   Size:       3488
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    983     983 
   Size:       3488
 
XmaI C'CCGG_G

Cuts at:    508     508 
   Size:       3488
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI     BglI    BmgBI     BmtI
     BpmI   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI
     ZraI
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