Restriction map for
pF1KB6802
(Circular) (Six-base) MAPSORT of: pF1KB6802.seq Check: 2427 from: 1 to: 3665
pF1KB6802 3665 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 703 703
Size: 3665
Acc65I G'GTAC_C
Cuts at: 681 681
Size: 3665
AclI AA'CG_TT
Cuts at: 3505 3505
Size: 3665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 652 1490 1922 2669 2937 577
Size: 75 838 432 747 268 1305
Fragments arranged by size:
1305 838 747 432 268 75
AflIII A'CryG_T
Cuts at: 1017 2121 3098 1017
Size: 1104 977 1584
Fragments arranged by size:
1584 1104 977
AgeI A'CCGG_T
Cuts at: 2053 2053
Size: 3665
AleI CACnn'nnGTG
Cuts at: 224 224
Size: 3665
AlwNI CAG_nnn'CTG
Cuts at: 555 970 2537 555
Size: 415 1567 1683
Fragments arranged by size:
1683 1567 415
ApaLI G'TGCA_C
Cuts at: 2435 2435
Size: 3665
ApoI r'AATT_y
Cuts at: 322 626 669 3018 3274 322
Size: 304 43 2349 256 713
Fragments arranged by size:
2349 713 304 256 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 3665
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
AvaI C'yCGr_G
Cuts at: 685 685
Size: 3665
BamHI G'GATC_C
Cuts at: 690 690
Size: 3665
BanI G'GyrC_C
Cuts at: 681 1095 1343 1378 681
Size: 414 248 35 2968
Fragments arranged by size:
2968 414 248 35
BanII G_rGCy'C
Cuts at: 580 679 1709 580
Size: 99 1030 2536
Fragments arranged by size:
2536 1030 99
BbeI G_GCGC'C
Cuts at: 1099 1347 1099
Size: 248 3417
BbsI GAAGACnn'nnnn_
Cuts at: 660 660
Size: 3665
BciVI GTATCCnnnnn_n'
Cuts at: 1563 2324 3665 1563
Size: 761 1341 1563
Fragments arranged by size:
1563 1341 761
BclI T'GATC_A
Cuts at: 1185 1185
Size: 3665
BglII A'GATC_T
Cuts at: 1180 3217 1180
Size: 2037 1628
BlpI GC'TnA_GC
Cuts at: 777 777
Size: 3665
Bme1580I G_kGCm'C
Cuts at: 1290 1383 2439 1290
Size: 93 1056 2516
Fragments arranged by size:
2516 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 877 958 1293 3437 877
Size: 81 335 2144 1105
Fragments arranged by size:
2144 1105 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 3665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 496 369
Size: 127 3538
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1720 2212 2510 841
Size: 879 492 298 1996
Fragments arranged by size:
1996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 387 3369 387
Size: 2982 683
BsaAI yAC'GTr
Cuts at: 1018 1018
Size: 3665
BsaBI GATnn'nnATC
Cuts at: 6 1179 3216 6
Size: 1173 2037 455
Fragments arranged by size:
2037 1173 455
BsaHI Gr'CG_yC
Cuts at: 1096 1344 2046 3542 1096
Size: 248 702 1496 1219
Fragments arranged by size:
1496 1219 702 248
BsaWI w'CCGG_w
Cuts at: 854 1375 2053 2327 2474 2885 854
Size: 521 678 274 147 411 1634
Fragments arranged by size:
1634 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 462 492 462
Size: 30 3635
BseYI C'CCAG_C
Cuts at: 267 437 466 1090 1848 2425 2921 267
Size: 170 29 624 758 577 496 1011
Fragments arranged by size:
1011 758 624 577 496 170 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 436
Size: 3665
BsiEI CG_ry'CG
Cuts at: 104 1207 1253 2461 104
Size: 1103 46 1208 1308
Fragments arranged by size:
1308 1208 1103 46
BsiHKAI G_wGCw'C
Cuts at: 679 1457 1647 2439 679
Size: 778 190 792 1905
Fragments arranged by size:
1905 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 164 164
Size: 3665
Bsp1286I G_dGCh'C
Cuts at: 580 679 1290 1383 1457 1647 1709 2439 580
Size: 99 611 93 74 190 62 730 1806
Fragments arranged by size:
1806 730 611 190 99 93 74 62
BspEI T'CCGG_A
Cuts at: 2885 2885
Size: 3665
BspHI T'CATG_A
Cuts at: 416 2841 416
Size: 2425 1240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 715 929 1231 1612 715
Size: 214 302 381 2768
Fragments arranged by size:
2768 381 302 214
BsrBI CCG'CTC
Cuts at: 50 619 1957 2011 3495 3660 50
Size: 569 1338 54 1484 165 55
Fragments arranged by size:
1484 1338 569 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1577 1577
Size: 3665
BsrFI r'CCGG_y
Cuts at: 338 1663 1844 2053 338
Size: 1325 181 209 1950
Fragments arranged by size:
1950 1325 209 181
BsrGI T'GTAC_A
Cuts at: 143 363 143
Size: 220 3445
BssSI C'ACGA_G
Cuts at: 331 1936 2294 331
Size: 1605 358 1702
Fragments arranged by size:
1702 1605 358
BstAPI GCAn_nnn'nTGC
Cuts at: 459 1042 459
Size: 583 3082
BstBI TT'CG_AA
Cuts at: 2026 2026
Size: 3665
BstYI r'GATC_y
Cuts at: 1 128 472 690 1157 1180 1515 1761 2773
Size: 127 344 218 467 23 335 246 1012
Cuts at: 2773 2859 2871 3217 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 344 335 246 218
127 86 23 12
BtgI C'CryG_G
Cuts at: 107 345 107
Size: 238 3427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1615 1796 1959 1615
Size: 181 163 3321
Fragments arranged by size:
3321 181 163
BtsI GCAGTG_nn'
Cuts at: 366 556 908 366
Size: 190 352 3123
Fragments arranged by size:
3123 352 190
DraI TTT'AAA
Cuts at: 664 664
Size: 3665
DraIII CAC_nnn'GTG
Cuts at: 459 459
Size: 3665
DrdI GACnn_nn'nnGTC
Cuts at: 1371 2229 1371
Size: 858 2807
EaeI y'GGCC_r
Cuts at: 374 614 1250 1424 1815 1842 3187 374
Size: 240 636 174 391 27 1345 852
Fragments arranged by size:
1345 852 636 391 240 174 27
EagI C'GGCC_G
Cuts at: 1250 1250
Size: 3665
EarI CTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1873 2183 2329 3475 1873
Size: 310 146 1146 2063
Fragments arranged by size:
2063 1146 310 146
EcoICRI GAG'CTC
Cuts at: 677 677
Size: 3665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 496 577 652 1490 1922 2669 2937 369
Size: 127 81 75 838 432 747 268 1097
Fragments arranged by size:
1097 838 747 432 268 127 81 75
EcoO109I rG'GnC_Cy
Cuts at: 804 804
Size: 3665
EcoRI G'AATT_C
Cuts at: 626 669 626
Size: 43 3622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1684 1684
Size: 3665
FspI TGC'GCA
Cuts at: 1446 1446
Size: 3665
HaeII r_GCGC'y
Cuts at: 1099 1347 2369 3348 1099
Size: 248 1022 979 1416
Fragments arranged by size:
1416 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 1507 1537 1539 1569 268
Size: 32 1207 30 2 30 2364
Fragments arranged by size:
2364 1207 32 30 30 2
HincII GTy'rAC
Cuts at: 704 704
Size: 3665
KasI G'GCGC_C
Cuts at: 1095 1343 1095
Size: 248 3417
KpnI G_GTAC'C
Cuts at: 685 685
Size: 3665
MfeI C'AATT_G
Cuts at: 181 181
Size: 3665
MluI A'CGCG_T
Cuts at: 3098 3098
Size: 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 221 2336 2520 221
Size: 2115 184 1366
Fragments arranged by size:
2115 1366 184
MscI TGG'CCA
Cuts at: 1426 1426
Size: 3665
MslI CAynn'nnrTG
Cuts at: 224 1654 1781 224
Size: 1430 127 2108
Fragments arranged by size:
2108 1430 127
MspA1I CmG'CkG
Cuts at: 274 347 776 1090 1450 2463 2708 274
Size: 73 429 314 360 1013 245 1231
Fragments arranged by size:
1231 1013 429 360 314 245 73
NaeI GCC'GGC
Cuts at: 340 1846 340
Size: 1506 2159
NarI GG'CG_CC
Cuts at: 1096 1344 1096
Size: 248 3417
NcoI C'CATG_G
Cuts at: 107 107
Size: 3665
NgoMIV G'CCGG_C
Cuts at: 338 1844 338
Size: 1506 2159
NspI r_CATG'y
Cuts at: 718 1218 2125 718
Size: 500 907 2258
Fragments arranged by size:
2258 907 500
PciI A'CATG_T
Cuts at: 2121 2121
Size: 3665
PfoI T'CCnGG_A
Cuts at: 385 512 621 385
Size: 127 109 3429
Fragments arranged by size:
3429 127 109
PmeI GTTT'AAAC
Cuts at: 664 664
Size: 3665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 3633
PstI C_TGCA'G
Cuts at: 712 1397 712
Size: 685 2980
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3665
PvuII CAG'CTG
Cuts at: 1090 1450 1090
Size: 360 3305
RsrII CG'GwC_CG
Cuts at: 1860 1860
Size: 3665
SacI G_AGCT'C
Cuts at: 679 679
Size: 3665
SacII CC_GC'GG
Cuts at: 348 348
Size: 3665
SalI G'TCGA_C
Cuts at: 702 702
Size: 3665
SapI GCTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
SbfI CC_TGCA'GG
Cuts at: 712 712
Size: 3665
ScaI AGT'ACT
Cuts at: 3182 3182
Size: 3665
SfcI C'TryA_G
Cuts at: 33 708 1393 2386 2577 33
Size: 675 685 993 191 1121
Fragments arranged by size:
1121 993 685 675 191
SfoI GGC'GCC
Cuts at: 1097 1345 1097
Size: 248 3417
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
SmaI CCC'GGG
Cuts at: 687 687
Size: 3665
SmlI C'TyrA_G
Cuts at: 820 1735 2227 2489 820
Size: 915 492 262 1996
Fragments arranged by size:
1996 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3665
SphI G_CATG'C
Cuts at: 718 1218 718
Size: 500 3165
StuI AGG'CCT
Cuts at: 234 234
Size: 3665
StyI C'CwwG_G
Cuts at: 107 799 1153 107
Size: 692 354 2619
Fragments arranged by size:
2619 692 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1248 1381 1840 2049 1248
Size: 133 459 209 2864
Fragments arranged by size:
2864 459 209 133
TatI w'GTAC_w
Cuts at: 143 363 3180 143
Size: 220 2817 628
Fragments arranged by size:
2817 628 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 462 494 3182 3214 462
Size: 32 2688 32 913
Fragments arranged by size:
2688 913 32 32
Tth111I GACn'n_nGTC
Cuts at: 1462 1462
Size: 3665
XbaI T'CTAG_A
Cuts at: 696 696
Size: 3665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 237 489 609 1160 237
Size: 252 120 551 2742
Fragments arranged by size:
2742 551 252 120
XmaI C'CCGG_G
Cuts at: 685 685
Size: 3665
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI