Restriction map for
pF1KB6809
(Circular) (Six-base) MAPSORT of: pF1KB6809.seq Check: 2366 from: 1 to: 3590
pF1KB6809 3590 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 425 628 425
Size: 203 3387
Acc65I G'GTAC_C
Cuts at: 606 606
Size: 3590
AclI AA'CG_TT
Cuts at: 3430 3430
Size: 3590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 1415 1847 2594 2862 151
Size: 1264 432 747 268 879
Fragments arranged by size:
1264 879 747 432 268
AflIII A'CryG_T
Cuts at: 431 942 2046 3023 431
Size: 511 1104 977 998
Fragments arranged by size:
1104 998 977 511
AgeI A'CCGG_T
Cuts at: 1978 1978
Size: 3590
AlwNI CAG_nnn'CTG
Cuts at: 895 2462 895
Size: 1567 2023
ApaLI G'TGCA_C
Cuts at: 316 2360 316
Size: 2044 1546
ApoI r'AATT_y
Cuts at: 594 2943 3199 594
Size: 2349 256 985
Fragments arranged by size:
2349 985 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
AvaI C'yCGr_G
Cuts at: 575 610 575
Size: 35 3555
AvrII C'CTAG_G
Cuts at: 144 144
Size: 3590
BamHI G'GATC_C
Cuts at: 615 615
Size: 3590
BanI G'GyrC_C
Cuts at: 528 606 1020 1268 1303 528
Size: 78 414 248 35 2815
Fragments arranged by size:
2815 414 248 78 35
BanII G_rGCy'C
Cuts at: 458 604 1634 458
Size: 146 1030 2414
Fragments arranged by size:
2414 1030 146
BbeI G_GCGC'C
Cuts at: 532 1024 1272 532
Size: 492 248 2850
Fragments arranged by size:
2850 492 248
BbsI GAAGACnn'nnnn_
Cuts at: 476 476
Size: 3590
BbvCI CC'TCA_GC
Cuts at: 388 388
Size: 3590
BciVI GTATCCnnnnn_n'
Cuts at: 1488 2249 3590 1488
Size: 761 1341 1488
Fragments arranged by size:
1488 1341 761
BclI T'GATC_A
Cuts at: 1110 1110
Size: 3590
BglI GCCn_nnn'nGGC
Cuts at: 368 368
Size: 3590
BglII A'GATC_T
Cuts at: 1105 3142 1105
Size: 2037 1553
BlpI GC'TnA_GC
Cuts at: 245 702 245
Size: 457 3133
Bme1580I G_kGCm'C
Cuts at: 320 1215 1308 2364 320
Size: 895 93 1056 1546
Fragments arranged by size:
1546 1056 895 93
BmrI ACTGGGnnnn_n'
Cuts at: 802 883 1218 3362 802
Size: 81 335 2144 1030
Fragments arranged by size:
2144 1030 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1957 1957
Size: 3590
Bpu10I CC'TnA_GC
Cuts at: 388 388
Size: 3590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 766 1645 2137 2435 385
Size: 381 879 492 298 1540
Fragments arranged by size:
1540 879 492 381 298
BsaI GGTCTCn'nnnn_
Cuts at: 3294 3294
Size: 3590
BsaAI yAC'GTr
Cuts at: 943 943
Size: 3590
BsaBI GATnn'nnATC
Cuts at: 6 1104 3141 6
Size: 1098 2037 455
Fragments arranged by size:
2037 1098 455
BsaHI Gr'CG_yC
Cuts at: 263 529 1021 1269 1971 3467 263
Size: 266 492 248 702 1496 386
Fragments arranged by size:
1496 702 492 386 266 248
BsaWI w'CCGG_w
Cuts at: 779 1300 1978 2252 2399 2810 779
Size: 521 678 274 147 411 1559
Fragments arranged by size:
1559 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1015 1773 2350 2846 1015
Size: 758 577 496 1759
Fragments arranged by size:
1759 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1132 1178 2386 104
Size: 1028 46 1208 1308
Fragments arranged by size:
1308 1208 1028 46
BsiHKAI G_wGCw'C
Cuts at: 320 458 604 1382 1572 2364 320
Size: 138 146 778 190 792 1546
Fragments arranged by size:
1546 792 778 190 146 138
Bsp1286I G_dGCh'C
Cuts at: 320 458 604 1215 1308 1382 1572 1634 2364
Size: 138 146 611 93 74 190 62 730
Cuts at: 2364 320
Size: 1546
Fragments arranged by size:
1546 730 611 190 146 138 93 74
62
BspEI T'CCGG_A
Cuts at: 2810 2810
Size: 3590
BspHI T'CATG_A
Cuts at: 2766 2766
Size: 3590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 640 854 1156 1537 640
Size: 214 302 381 2693
Fragments arranged by size:
2693 381 302 214
BsrBI CCG'CTC
Cuts at: 50 408 1882 1936 3420 3585 50
Size: 358 1474 54 1484 165 55
Fragments arranged by size:
1484 1474 358 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1502 1502
Size: 3590
BsrFI r'CCGG_y
Cuts at: 1588 1769 1978 1588
Size: 181 209 3200
Fragments arranged by size:
3200 209 181
BssSI C'ACGA_G
Cuts at: 1861 2219 1861
Size: 358 3232
BstAPI GCAn_nnn'nTGC
Cuts at: 967 967
Size: 3590
BstBI TT'CG_AA
Cuts at: 1951 1951
Size: 3590
BstXI CCAn_nnnn'nTGG
Cuts at: 306 306
Size: 3590
BstYI r'GATC_y
Cuts at: 1 537 615 1082 1105 1440 1686 2698 2784
Size: 536 78 467 23 335 246 1012 86
Cuts at: 2784 2796 3142 1
Size: 12 346 449
Fragments arranged by size:
1012 536 467 449 346 335 246 86
78 23 12
BtgI C'CryG_G
Cuts at: 107 337 107
Size: 230 3360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1540 1721 1884 1540
Size: 181 163 3246
Fragments arranged by size:
3246 181 163
BtsI GCAGTG_nn'
Cuts at: 833 833
Size: 3590
DraI TTT'AAA
Cuts at: 589 589
Size: 3590
DrdI GACnn_nn'nnGTC
Cuts at: 1296 2154 1296
Size: 858 2732
EaeI y'GGCC_r
Cuts at: 293 380 1175 1349 1740 1767 3112 293
Size: 87 795 174 391 27 1345 771
Fragments arranged by size:
1345 795 771 391 174 87 27
EagI C'GGCC_G
Cuts at: 1175 1175
Size: 3590
EarI CTCTTCn'nnn_
Cuts at: 1613 1823 1613
Size: 210 3380
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1798 2108 2254 3400 1798
Size: 310 146 1146 1988
Fragments arranged by size:
1988 1146 310 146
EcoICRI GAG'CTC
Cuts at: 456 602 456
Size: 146 3444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 1415 1847 2594 2862 151
Size: 1264 432 747 268 879
Fragments arranged by size:
1264 879 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 729 729
Size: 3590
EcoRI G'AATT_C
Cuts at: 594 594
Size: 3590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1609 1609
Size: 3590
FspI TGC'GCA
Cuts at: 1371 1371
Size: 3590
HaeII r_GCGC'y
Cuts at: 532 1024 1272 2294 3273 532
Size: 492 248 1022 979 849
Fragments arranged by size:
1022 979 849 492 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1432 1462 1464 1494 1432
Size: 30 2 30 3528
Fragments arranged by size:
3528 30 30 2
HincII GTy'rAC
Cuts at: 629 629
Size: 3590
KasI G'GCGC_C
Cuts at: 528 1020 1268 528
Size: 492 248 2850
Fragments arranged by size:
2850 492 248
KpnI G_GTAC'C
Cuts at: 610 610
Size: 3590
MluI A'CGCG_T
Cuts at: 3023 3023
Size: 3590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2261 2445 2261
Size: 184 3406
MscI TGG'CCA
Cuts at: 295 382 1351 295
Size: 87 969 2534
Fragments arranged by size:
2534 969 87
MslI CAynn'nnrTG
Cuts at: 271 1579 1706 271
Size: 1308 127 2155
Fragments arranged by size:
2155 1308 127
MspA1I CmG'CkG
Cuts at: 249 339 701 1015 1375 2388 2633 249
Size: 90 362 314 360 1013 245 1206
Fragments arranged by size:
1206 1013 362 360 314 245 90
NaeI GCC'GGC
Cuts at: 1771 1771
Size: 3590
NarI GG'CG_CC
Cuts at: 529 1021 1269 529
Size: 492 248 2850
Fragments arranged by size:
2850 492 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3590
NgoMIV G'CCGG_C
Cuts at: 1769 1769
Size: 3590
NspI r_CATG'y
Cuts at: 276 435 643 1143 2050 276
Size: 159 208 500 907 1816
Fragments arranged by size:
1816 907 500 208 159
PciI A'CATG_T
Cuts at: 431 2046 431
Size: 1615 1975
PfoI T'CCnGG_A
Cuts at: 305 305
Size: 3590
PmeI GTTT'AAAC
Cuts at: 589 589
Size: 3590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2729 2761 2729
Size: 32 3558
PstI C_TGCA'G
Cuts at: 637 1322 637
Size: 685 2905
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3590
PvuII CAG'CTG
Cuts at: 1015 1375 1015
Size: 360 3230
RsrII CG'GwC_CG
Cuts at: 1785 1785
Size: 3590
SacI G_AGCT'C
Cuts at: 458 604 458
Size: 146 3444
SacII CC_GC'GG
Cuts at: 340 340
Size: 3590
SalI G'TCGA_C
Cuts at: 627 627
Size: 3590
SapI GCTCTTCn'nnn_
Cuts at: 1613 1823 1613
Size: 210 3380
SbfI CC_TGCA'GG
Cuts at: 637 637
Size: 3590
ScaI AGT'ACT
Cuts at: 3107 3107
Size: 3590
SfcI C'TryA_G
Cuts at: 33 633 1318 2311 2502 33
Size: 600 685 993 191 1121
Fragments arranged by size:
1121 993 685 600 191
SfoI GGC'GCC
Cuts at: 530 1022 1270 530
Size: 492 248 2850
Fragments arranged by size:
2850 492 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3590
SmaI CCC'GGG
Cuts at: 612 612
Size: 3590
SmlI C'TyrA_G
Cuts at: 400 745 1660 2152 2414 400
Size: 345 915 492 262 1576
Fragments arranged by size:
1576 915 492 345 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3590
SphI G_CATG'C
Cuts at: 643 1143 643
Size: 500 3090
StyI C'CwwG_G
Cuts at: 107 144 224 477 724 1078 107
Size: 37 80 253 247 354 2619
Fragments arranged by size:
2619 354 253 247 80 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 1173 1306 1765 1974 271
Size: 902 133 459 209 1887
Fragments arranged by size:
1887 902 459 209 133
TatI w'GTAC_w
Cuts at: 3105 3105
Size: 3590
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 3558
Tth111I GACn'n_nGTC
Cuts at: 1387 1387
Size: 3590
XbaI T'CTAG_A
Cuts at: 621 621
Size: 3590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 222 303 1085 222
Size: 81 782 2727
Fragments arranged by size:
2727 782 81
XmaI C'CCGG_G
Cuts at: 610 610
Size: 3590
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BcgI BfrBI BmgBI BmtI BpmI
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI