Restriction map for
pF1KB6812
(Circular) (Six-base) MAPSORT of: pF1KB6812.seq Check: 119 from: 1 to: 3668
pF1KB6812 3668 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 706 706
Size: 3668
Acc65I G'GTAC_C
Cuts at: 684 684
Size: 3668
AclI AA'CG_TT
Cuts at: 3508 3508
Size: 3668
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 1493 1925 2672 2940 218
Size: 1275 432 747 268 946
Fragments arranged by size:
1275 946 747 432 268
AflIII A'CryG_T
Cuts at: 516 1020 2124 3101 516
Size: 504 1104 977 1083
Fragments arranged by size:
1104 1083 977 504
AgeI A'CCGG_T
Cuts at: 2056 2056
Size: 3668
AlwNI CAG_nnn'CTG
Cuts at: 324 414 973 2540 324
Size: 90 559 1567 1452
Fragments arranged by size:
1567 1452 559 90
ApaI G_GGCC'C
Cuts at: 184 232 301 184
Size: 48 69 3551
Fragments arranged by size:
3551 69 48
ApaLI G'TGCA_C
Cuts at: 2438 2438
Size: 3668
ApoI r'AATT_y
Cuts at: 672 3021 3277 672
Size: 2349 256 1063
Fragments arranged by size:
2349 1063 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3668
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
AvaI C'yCGr_G
Cuts at: 584 688 584
Size: 104 3564
BamHI G'GATC_C
Cuts at: 693 693
Size: 3668
BanI G'GyrC_C
Cuts at: 133 360 684 1098 1346 1381 133
Size: 227 324 414 248 35 2420
Fragments arranged by size:
2420 414 324 248 227 35
BanII G_rGCy'C
Cuts at: 184 232 301 585 682 1712 184
Size: 48 69 284 97 1030 2140
Fragments arranged by size:
2140 1030 284 97 69 48
BbeI G_GCGC'C
Cuts at: 137 1102 1350 137
Size: 965 248 2455
Fragments arranged by size:
2455 965 248
BciVI GTATCCnnnnn_n'
Cuts at: 1566 2327 3668 1566
Size: 761 1341 1566
Fragments arranged by size:
1566 1341 761
BclI T'GATC_A
Cuts at: 1188 1188
Size: 3668
BglI GCCn_nnn'nGGC
Cuts at: 120 148 305 643 120
Size: 28 157 338 3145
Fragments arranged by size:
3145 338 157 28
BglII A'GATC_T
Cuts at: 1183 3220 1183
Size: 2037 1631
BlpI GC'TnA_GC
Cuts at: 780 780
Size: 3668
Bme1580I G_kGCm'C
Cuts at: 184 232 301 1293 1386 2442 184
Size: 48 69 992 93 1056 1410
Fragments arranged by size:
1410 1056 992 93 69 48
BmrI ACTGGGnnnn_n'
Cuts at: 226 254 597 880 961 1296 3440 226
Size: 28 343 283 81 335 2144 454
Fragments arranged by size:
2144 454 343 335 283 81 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 3668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 566
Size: 3668
Bpu10I CC'TnA_GC
Cuts at: 185 185
Size: 3668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1723 2215 2513 844
Size: 879 492 298 1999
Fragments arranged by size:
1999 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3372 3372
Size: 3668
BsaAI yAC'GTr
Cuts at: 1021 1021
Size: 3668
BsaBI GATnn'nnATC
Cuts at: 6 1182 3219 6
Size: 1176 2037 455
Fragments arranged by size:
2037 1176 455
BsaHI Gr'CG_yC
Cuts at: 134 1099 1347 2049 3545 134
Size: 965 248 702 1496 257
Fragments arranged by size:
1496 965 702 257 248
BsaWI w'CCGG_w
Cuts at: 857 1378 2056 2330 2477 2888 857
Size: 521 678 274 147 411 1637
Fragments arranged by size:
1637 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 368 398 368
Size: 30 3638
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 182 277 361 478 182
Size: 95 84 117 3372
Fragments arranged by size:
3372 117 95 84
BseYI C'CCAG_C
Cuts at: 300 1093 1851 2428 2924 300
Size: 793 758 577 496 1044
Fragments arranged by size:
1044 793 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1210 1256 2464 104
Size: 1106 46 1208 1308
Fragments arranged by size:
1308 1208 1106 46
BsiHKAI G_wGCw'C
Cuts at: 682 1460 1650 2442 682
Size: 778 190 792 1908
Fragments arranged by size:
1908 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 184 232 301 585 682 1293 1386 1460 1650
Size: 48 69 284 97 611 93 74 190
Cuts at: 1650 1712 2442 184
Size: 62 730 1410
Fragments arranged by size:
1410 730 611 284 190 97 93 74
69 62 48
BspEI T'CCGG_A
Cuts at: 2888 2888
Size: 3668
BspHI T'CATG_A
Cuts at: 2844 2844
Size: 3668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 718 932 1234 1615 718
Size: 214 302 381 2771
Fragments arranged by size:
2771 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1960 2014 3498 3663 50
Size: 1910 54 1484 165 55
Fragments arranged by size:
1910 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1580 1580
Size: 3668
BsrFI r'CCGG_y
Cuts at: 1666 1847 2056 1666
Size: 181 209 3278
Fragments arranged by size:
3278 209 181
BssSI C'ACGA_G
Cuts at: 1939 2297 1939
Size: 358 3310
BstAPI GCAn_nnn'nTGC
Cuts at: 652 1045 652
Size: 393 3275
BstBI TT'CG_AA
Cuts at: 2029 2029
Size: 3668
BstYI r'GATC_y
Cuts at: 1 333 693 1160 1183 1518 1764 2776 2862
Size: 332 360 467 23 335 246 1012 86
Cuts at: 2862 2874 3220 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 360 346 335 332 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 269 269
Size: 3668
BtgI C'CryG_G
Cuts at: 107 176 503 107
Size: 69 327 3272
Fragments arranged by size:
3272 327 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1618 1799 1962 1618
Size: 181 163 3324
Fragments arranged by size:
3324 181 163
BtsI GCAGTG_nn'
Cuts at: 264 911 264
Size: 647 3021
DraI TTT'AAA
Cuts at: 667 667
Size: 3668
DrdI GACnn_nn'nnGTC
Cuts at: 1374 2232 1374
Size: 858 2810
EaeI y'GGCC_r
Cuts at: 1253 1427 1818 1845 3190 1253
Size: 174 391 27 1345 1731
Fragments arranged by size:
1731 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1253 1253
Size: 3668
EarI CTCTTCn'nnn_
Cuts at: 269 1691 1901 269
Size: 1422 210 2036
Fragments arranged by size:
2036 1422 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1876 2186 2332 3478 1876
Size: 310 146 1146 2066
Fragments arranged by size:
2066 1146 310 146
EcoICRI GAG'CTC
Cuts at: 680 680
Size: 3668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 566 1493 1925 2672 2940 218
Size: 348 927 432 747 268 946
Fragments arranged by size:
946 927 747 432 348 268
EcoNI CCTnn'n_nnAGG
Cuts at: 236 563 236
Size: 327 3341
EcoO109I rG'GnC_Cy
Cuts at: 112 181 228 229 411 807 112
Size: 69 47 1 182 396 2973
Fragments arranged by size:
2973 396 182 69 47 1
EcoRI G'AATT_C
Cuts at: 672 672
Size: 3668
EcoRV GAT'ATC
Cuts at: 593 593
Size: 3668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1687 1687
Size: 3668
FspI TGC'GCA
Cuts at: 1449 1449
Size: 3668
HaeII r_GCGC'y
Cuts at: 137 1102 1350 2372 3351 137
Size: 965 248 1022 979 454
Fragments arranged by size:
1022 979 965 454 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1510 1540 1542 1572 1510
Size: 30 2 30 3606
Fragments arranged by size:
3606 30 30 2
HincII GTy'rAC
Cuts at: 707 707
Size: 3668
KasI G'GCGC_C
Cuts at: 133 1098 1346 133
Size: 965 248 2455
Fragments arranged by size:
2455 965 248
KpnI G_GTAC'C
Cuts at: 688 688
Size: 3668
MluI A'CGCG_T
Cuts at: 3101 3101
Size: 3668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2523 2339
Size: 184 3484
MscI TGG'CCA
Cuts at: 1429 1429
Size: 3668
MslI CAynn'nnrTG
Cuts at: 1657 1784 1657
Size: 127 3541
MspA1I CmG'CkG
Cuts at: 304 779 1093 1453 2466 2711 304
Size: 475 314 360 1013 245 1261
Fragments arranged by size:
1261 1013 475 360 314 245
NaeI GCC'GGC
Cuts at: 1849 1849
Size: 3668
NarI GG'CG_CC
Cuts at: 134 1099 1347 134
Size: 965 248 2455
Fragments arranged by size:
2455 965 248
NcoI C'CATG_G
Cuts at: 107 176 503 107
Size: 69 327 3272
Fragments arranged by size:
3272 327 69
NgoMIV G'CCGG_C
Cuts at: 1847 1847
Size: 3668
NspI r_CATG'y
Cuts at: 520 721 1221 2128 520
Size: 201 500 907 2060
Fragments arranged by size:
2060 907 500 201
PasI CC'CwG_GG
Cuts at: 414 414
Size: 3668
PciI A'CATG_T
Cuts at: 516 2124 516
Size: 1608 2060
PflMI CCAn_nnn'nTGG
Cuts at: 414 414
Size: 3668
PfoI T'CCnGG_A
Cuts at: 395 395
Size: 3668
PmeI GTTT'AAAC
Cuts at: 667 667
Size: 3668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 3636
PspOMI G'GGCC_C
Cuts at: 180 228 297 180
Size: 48 69 3551
Fragments arranged by size:
3551 69 48
PstI C_TGCA'G
Cuts at: 715 1400 715
Size: 685 2983
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3668
PvuII CAG'CTG
Cuts at: 304 1093 1453 304
Size: 789 360 2519
Fragments arranged by size:
2519 789 360
RsrII CG'GwC_CG
Cuts at: 1863 1863
Size: 3668
SacI G_AGCT'C
Cuts at: 682 682
Size: 3668
SalI G'TCGA_C
Cuts at: 705 705
Size: 3668
SapI GCTCTTCn'nnn_
Cuts at: 269 1691 1901 269
Size: 1422 210 2036
Fragments arranged by size:
2036 1422 210
SbfI CC_TGCA'GG
Cuts at: 715 715
Size: 3668
ScaI AGT'ACT
Cuts at: 3185 3185
Size: 3668
SfcI C'TryA_G
Cuts at: 33 473 711 1396 2389 2580 33
Size: 440 238 685 993 191 1121
Fragments arranged by size:
1121 993 685 440 238 191
SfoI GGC'GCC
Cuts at: 135 1100 1348 135
Size: 965 248 2455
Fragments arranged by size:
2455 965 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
SmaI CCC'GGG
Cuts at: 690 690
Size: 3668
SmlI C'TyrA_G
Cuts at: 823 1738 2230 2492 823
Size: 915 492 262 1999
Fragments arranged by size:
1999 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3668
SphI G_CATG'C
Cuts at: 721 1221 721
Size: 500 3168
StyI C'CwwG_G
Cuts at: 107 176 503 802 1156 107
Size: 69 327 299 354 2619
Fragments arranged by size:
2619 354 327 299 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1251 1384 1843 2052 1251
Size: 133 459 209 2867
Fragments arranged by size:
2867 459 209 133
TatI w'GTAC_w
Cuts at: 3183 3183
Size: 3668
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 589 621 3185 3217 589
Size: 32 2564 32 1040
Fragments arranged by size:
2564 1040 32 32
Tth111I GACn'n_nGTC
Cuts at: 1465 1465
Size: 3668
XbaI T'CTAG_A
Cuts at: 699 699
Size: 3668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1163 1163
Size: 3668
XmaI C'CCGG_G
Cuts at: 688 688
Size: 3668
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI