Restriction map for
pF1KB6814
(Circular) (Six-base) MAPSORT of: pF1KB6814.seq Check: 1162 from: 1 to: 3668
pF1KB6814 3668 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 706 706
Size: 3668
Acc65I G'GTAC_C
Cuts at: 684 684
Size: 3668
AclI AA'CG_TT
Cuts at: 3508 3508
Size: 3668
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1493 1925 2672 2940 1493
Size: 432 747 268 2221
Fragments arranged by size:
2221 747 432 268
AflIII A'CryG_T
Cuts at: 1020 2124 3101 1020
Size: 1104 977 1587
Fragments arranged by size:
1587 1104 977
AgeI A'CCGG_T
Cuts at: 2056 2056
Size: 3668
AlwNI CAG_nnn'CTG
Cuts at: 973 2540 973
Size: 1567 2101
ApaLI G'TGCA_C
Cuts at: 2438 2438
Size: 3668
ApoI r'AATT_y
Cuts at: 428 613 672 3021 3277 428
Size: 185 59 2349 256 819
Fragments arranged by size:
2349 819 256 185 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 3668
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
AvaI C'yCGr_G
Cuts at: 688 688
Size: 3668
BamHI G'GATC_C
Cuts at: 693 693
Size: 3668
BanI G'GyrC_C
Cuts at: 684 1098 1346 1381 684
Size: 414 248 35 2971
Fragments arranged by size:
2971 414 248 35
BanII G_rGCy'C
Cuts at: 604 682 1712 604
Size: 78 1030 2560
Fragments arranged by size:
2560 1030 78
BbeI G_GCGC'C
Cuts at: 1102 1350 1102
Size: 248 3420
BbsI GAAGACnn'nnnn_
Cuts at: 337 337
Size: 3668
BciVI GTATCCnnnnn_n'
Cuts at: 1566 2327 3668 1566
Size: 761 1341 1566
Fragments arranged by size:
1566 1341 761
BclI T'GATC_A
Cuts at: 1188 1188
Size: 3668
BglII A'GATC_T
Cuts at: 386 1183 3220 386
Size: 797 2037 834
Fragments arranged by size:
2037 834 797
BlpI GC'TnA_GC
Cuts at: 780 780
Size: 3668
Bme1580I G_kGCm'C
Cuts at: 1293 1386 2442 1293
Size: 93 1056 2519
Fragments arranged by size:
2519 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 880 961 1296 3440 880
Size: 81 335 2144 1108
Fragments arranged by size:
2144 1108 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 3668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 844 1723 2215 2513 460
Size: 384 879 492 298 1615
Fragments arranged by size:
1615 879 492 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 3372 3372
Size: 3668
BsaAI yAC'GTr
Cuts at: 1021 1021
Size: 3668
BsaBI GATnn'nnATC
Cuts at: 6 251 1182 3219 6
Size: 245 931 2037 455
Fragments arranged by size:
2037 931 455 245
BsaHI Gr'CG_yC
Cuts at: 1099 1347 2049 3545 1099
Size: 248 702 1496 1222
Fragments arranged by size:
1496 1222 702 248
BsaWI w'CCGG_w
Cuts at: 857 1378 2056 2330 2477 2888 857
Size: 521 678 274 147 411 1637
Fragments arranged by size:
1637 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 284 1093 1851 2428 2924 284
Size: 809 758 577 496 1028
Fragments arranged by size:
1028 809 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1210 1256 2464 104
Size: 1106 46 1208 1308
Fragments arranged by size:
1308 1208 1106 46
BsiHKAI G_wGCw'C
Cuts at: 604 682 1460 1650 2442 604
Size: 78 778 190 792 1830
Fragments arranged by size:
1830 792 778 190 78
Bsp1286I G_dGCh'C
Cuts at: 604 682 1293 1386 1460 1650 1712 2442 604
Size: 78 611 93 74 190 62 730 1830
Fragments arranged by size:
1830 730 611 190 93 78 74 62
BspEI T'CCGG_A
Cuts at: 2888 2888
Size: 3668
BspHI T'CATG_A
Cuts at: 512 641 2844 512
Size: 129 2203 1336
Fragments arranged by size:
2203 1336 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 718 932 1234 1615 718
Size: 214 302 381 2771
Fragments arranged by size:
2771 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1960 2014 3498 3663 50
Size: 1910 54 1484 165 55
Fragments arranged by size:
1910 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1580 1580
Size: 3668
BsrFI r'CCGG_y
Cuts at: 1666 1847 2056 1666
Size: 181 209 3278
Fragments arranged by size:
3278 209 181
BsrGI T'GTAC_A
Cuts at: 625 625
Size: 3668
BssSI C'ACGA_G
Cuts at: 1939 2297 1939
Size: 358 3310
BstAPI GCAn_nnn'nTGC
Cuts at: 1045 1045
Size: 3668
BstBI TT'CG_AA
Cuts at: 2029 2029
Size: 3668
BstXI CCAn_nnnn'nTGG
Cuts at: 153 461 153
Size: 308 3360
BstYI r'GATC_y
Cuts at: 1 386 693 1160 1183 1518 1764 2776 2862
Size: 385 307 467 23 335 246 1012 86
Cuts at: 2862 2874 3220 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 385 346 335 307 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1618 1799 1962 1618
Size: 181 163 3324
Fragments arranged by size:
3324 181 163
BtsI GCAGTG_nn'
Cuts at: 911 911
Size: 3668
DraI TTT'AAA
Cuts at: 667 667
Size: 3668
DrdI GACnn_nn'nnGTC
Cuts at: 1374 2232 1374
Size: 858 2810
EaeI y'GGCC_r
Cuts at: 1253 1427 1818 1845 3190 1253
Size: 174 391 27 1345 1731
Fragments arranged by size:
1731 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1253 1253
Size: 3668
EarI CTCTTCn'nnn_
Cuts at: 546 590 1691 1901 546
Size: 44 1101 210 2313
Fragments arranged by size:
2313 1101 210 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1876 2186 2332 3478 1876
Size: 310 146 1146 2066
Fragments arranged by size:
2066 1146 310 146
EcoICRI GAG'CTC
Cuts at: 602 680 602
Size: 78 3590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1493 1925 2672 2940 1493
Size: 432 747 268 2221
Fragments arranged by size:
2221 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 807 807
Size: 3668
EcoRI G'AATT_C
Cuts at: 672 672
Size: 3668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1687 1687
Size: 3668
FspI TGC'GCA
Cuts at: 1449 1449
Size: 3668
HaeII r_GCGC'y
Cuts at: 1102 1350 2372 3351 1102
Size: 248 1022 979 1419
Fragments arranged by size:
1419 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 343 375 1510 1540 1542 1572 343
Size: 32 1135 30 2 30 2439
Fragments arranged by size:
2439 1135 32 30 30 2
HincII GTy'rAC
Cuts at: 707 707
Size: 3668
KasI G'GCGC_C
Cuts at: 1098 1346 1098
Size: 248 3420
KpnI G_GTAC'C
Cuts at: 688 688
Size: 3668
MluI A'CGCG_T
Cuts at: 3101 3101
Size: 3668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 134 2339 2523 134
Size: 2205 184 1279
Fragments arranged by size:
2205 1279 184
MscI TGG'CCA
Cuts at: 1429 1429
Size: 3668
MslI CAynn'nnrTG
Cuts at: 545 562 1657 1784 545
Size: 17 1095 127 2429
Fragments arranged by size:
2429 1095 127 17
MspA1I CmG'CkG
Cuts at: 779 1093 1453 2466 2711 779
Size: 314 360 1013 245 1736
Fragments arranged by size:
1736 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1849 1849
Size: 3668
NarI GG'CG_CC
Cuts at: 1099 1347 1099
Size: 248 3420
NdeI CA'TA_TG
Cuts at: 563 563
Size: 3668
NgoMIV G'CCGG_C
Cuts at: 1847 1847
Size: 3668
NspI r_CATG'y
Cuts at: 721 1221 2128 721
Size: 500 907 2261
Fragments arranged by size:
2261 907 500
PciI A'CATG_T
Cuts at: 2124 2124
Size: 3668
PflMI CCAn_nnn'nTGG
Cuts at: 226 226
Size: 3668
PmeI GTTT'AAAC
Cuts at: 667 667
Size: 3668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 3636
PstI C_TGCA'G
Cuts at: 715 1400 715
Size: 685 2983
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3668
PvuII CAG'CTG
Cuts at: 1093 1453 1093
Size: 360 3308
RsrII CG'GwC_CG
Cuts at: 1863 1863
Size: 3668
SacI G_AGCT'C
Cuts at: 604 682 604
Size: 78 3590
SalI G'TCGA_C
Cuts at: 705 705
Size: 3668
SapI GCTCTTCn'nnn_
Cuts at: 1691 1901 1691
Size: 210 3458
SbfI CC_TGCA'GG
Cuts at: 715 715
Size: 3668
ScaI AGT'ACT
Cuts at: 3185 3185
Size: 3668
SfcI C'TryA_G
Cuts at: 33 632 711 1396 2389 2580 33
Size: 599 79 685 993 191 1121
Fragments arranged by size:
1121 993 685 599 191 79
SfoI GGC'GCC
Cuts at: 1100 1348 1100
Size: 248 3420
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
SmaI CCC'GGG
Cuts at: 690 690
Size: 3668
SmlI C'TyrA_G
Cuts at: 439 823 1738 2230 2492 439
Size: 384 915 492 262 1615
Fragments arranged by size:
1615 915 492 384 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3668
SphI G_CATG'C
Cuts at: 721 1221 721
Size: 500 3168
StyI C'CwwG_G
Cuts at: 802 1156 802
Size: 354 3314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 574 1251 1384 1843 2052 574
Size: 677 133 459 209 2190
Fragments arranged by size:
2190 677 459 209 133
TatI w'GTAC_w
Cuts at: 625 3183 625
Size: 2558 1110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 215 247 3185 3217 215
Size: 32 2938 32 666
Fragments arranged by size:
2938 666 32 32
Tth111I GACn'n_nGTC
Cuts at: 1465 1465
Size: 3668
XbaI T'CTAG_A
Cuts at: 255 699 255
Size: 444 3224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1163 1163
Size: 3668
XmaI C'CCGG_G
Cuts at: 688 688
Size: 3668
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI