Restriction map for
pF1KB6816
(Circular) (Six-base) MAPSORT of: pF1KB6816.seq Check: 4185 from: 1 to: 3668
pF1KB6816 3668 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 319 420 706 319
Size: 101 286 3281
Fragments arranged by size:
3281 286 101
Acc65I G'GTAC_C
Cuts at: 684 684
Size: 3668
AclI AA'CG_TT
Cuts at: 3508 3508
Size: 3668
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 427 1493 1925 2672 2940 172
Size: 255 1066 432 747 268 900
Fragments arranged by size:
1066 900 747 432 268 255
AflIII A'CryG_T
Cuts at: 1020 2124 3101 1020
Size: 1104 977 1587
Fragments arranged by size:
1587 1104 977
AgeI A'CCGG_T
Cuts at: 2056 2056
Size: 3668
AlwNI CAG_nnn'CTG
Cuts at: 346 973 2540 346
Size: 627 1567 1474
Fragments arranged by size:
1567 1474 627
ApaLI G'TGCA_C
Cuts at: 2438 2438
Size: 3668
ApoI r'AATT_y
Cuts at: 473 672 3021 3277 473
Size: 199 2349 256 864
Fragments arranged by size:
2349 864 256 199
AseI AT'TA_AT
Cuts at: 20 20
Size: 3668
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
AvaI C'yCGr_G
Cuts at: 688 688
Size: 3668
BamHI G'GATC_C
Cuts at: 693 693
Size: 3668
BanI G'GyrC_C
Cuts at: 684 1098 1346 1381 684
Size: 414 248 35 2971
Fragments arranged by size:
2971 414 248 35
BanII G_rGCy'C
Cuts at: 682 1712 682
Size: 1030 2638
BbeI G_GCGC'C
Cuts at: 1102 1350 1102
Size: 248 3420
BciVI GTATCCnnnnn_n'
Cuts at: 297 1566 2327 3668 297
Size: 1269 761 1341 297
Fragments arranged by size:
1341 1269 761 297
BclI T'GATC_A
Cuts at: 1188 1188
Size: 3668
BglII A'GATC_T
Cuts at: 1183 3220 1183
Size: 2037 1631
BlpI GC'TnA_GC
Cuts at: 780 780
Size: 3668
Bme1580I G_kGCm'C
Cuts at: 1293 1386 2442 1293
Size: 93 1056 2519
Fragments arranged by size:
2519 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 880 961 1296 3440 880
Size: 81 335 2144 1108
Fragments arranged by size:
2144 1108 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 3668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1723 2215 2513 844
Size: 879 492 298 1999
Fragments arranged by size:
1999 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3372 3372
Size: 3668
BsaAI yAC'GTr
Cuts at: 1021 1021
Size: 3668
BsaBI GATnn'nnATC
Cuts at: 6 1182 3219 6
Size: 1176 2037 455
Fragments arranged by size:
2037 1176 455
BsaHI Gr'CG_yC
Cuts at: 1099 1347 2049 3545 1099
Size: 248 702 1496 1222
Fragments arranged by size:
1496 1222 702 248
BsaWI w'CCGG_w
Cuts at: 857 1378 2056 2330 2477 2888 857
Size: 521 678 274 147 411 1637
Fragments arranged by size:
1637 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1093 1851 2428 2924 1093
Size: 758 577 496 1837
Fragments arranged by size:
1837 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1210 1256 2464 104
Size: 1106 46 1208 1308
Fragments arranged by size:
1308 1208 1106 46
BsiHKAI G_wGCw'C
Cuts at: 682 1460 1650 2442 682
Size: 778 190 792 1908
Fragments arranged by size:
1908 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 682 1293 1386 1460 1650 1712 2442 682
Size: 611 93 74 190 62 730 1908
Fragments arranged by size:
1908 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2888 2888
Size: 3668
BspHI T'CATG_A
Cuts at: 624 2844 624
Size: 2220 1448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 718 932 1234 1615 718
Size: 214 302 381 2771
Fragments arranged by size:
2771 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1960 2014 3498 3663 50
Size: 1910 54 1484 165 55
Fragments arranged by size:
1910 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1580 1580
Size: 3668
BsrFI r'CCGG_y
Cuts at: 1666 1847 2056 1666
Size: 181 209 3278
Fragments arranged by size:
3278 209 181
BssSI C'ACGA_G
Cuts at: 1939 2297 1939
Size: 358 3310
BstAPI GCAn_nnn'nTGC
Cuts at: 1045 1045
Size: 3668
BstBI TT'CG_AA
Cuts at: 2029 2029
Size: 3668
BstYI r'GATC_y
Cuts at: 1 693 1160 1183 1518 1764 2776 2862 2874
Size: 692 467 23 335 246 1012 86 12
Cuts at: 2874 3220 1
Size: 346 449
Fragments arranged by size:
1012 692 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1618 1799 1962 1618
Size: 181 163 3324
Fragments arranged by size:
3324 181 163
BtsI GCAGTG_nn'
Cuts at: 213 617 911 213
Size: 404 294 2970
Fragments arranged by size:
2970 404 294
DraI TTT'AAA
Cuts at: 667 667
Size: 3668
DrdI GACnn_nn'nnGTC
Cuts at: 1374 2232 1374
Size: 858 2810
EaeI y'GGCC_r
Cuts at: 1253 1427 1818 1845 3190 1253
Size: 174 391 27 1345 1731
Fragments arranged by size:
1731 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1253 1253
Size: 3668
EarI CTCTTCn'nnn_
Cuts at: 1691 1901 1691
Size: 210 3458
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1876 2186 2332 3478 1876
Size: 310 146 1146 2066
Fragments arranged by size:
2066 1146 310 146
EcoICRI GAG'CTC
Cuts at: 680 680
Size: 3668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 427 1493 1925 2672 2940 172
Size: 255 1066 432 747 268 900
Fragments arranged by size:
1066 900 747 432 268 255
EcoO109I rG'GnC_Cy
Cuts at: 807 807
Size: 3668
EcoRI G'AATT_C
Cuts at: 672 672
Size: 3668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1687 1687
Size: 3668
FspI TGC'GCA
Cuts at: 1449 1449
Size: 3668
HaeII r_GCGC'y
Cuts at: 1102 1350 2372 3351 1102
Size: 248 1022 979 1419
Fragments arranged by size:
1419 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 1510 1540 1542 1572 373
Size: 32 1105 30 2 30 2469
Fragments arranged by size:
2469 1105 32 30 30 2
HincII GTy'rAC
Cuts at: 707 707
Size: 3668
KasI G'GCGC_C
Cuts at: 1098 1346 1098
Size: 248 3420
KpnI G_GTAC'C
Cuts at: 688 688
Size: 3668
MluI A'CGCG_T
Cuts at: 3101 3101
Size: 3668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 626 2339 2523 626
Size: 1713 184 1771
Fragments arranged by size:
1771 1713 184
MscI TGG'CCA
Cuts at: 1429 1429
Size: 3668
MslI CAynn'nnrTG
Cuts at: 1657 1784 1657
Size: 127 3541
MspA1I CmG'CkG
Cuts at: 447 779 1093 1453 2466 2711 447
Size: 332 314 360 1013 245 1404
Fragments arranged by size:
1404 1013 360 332 314 245
NaeI GCC'GGC
Cuts at: 1849 1849
Size: 3668
NarI GG'CG_CC
Cuts at: 1099 1347 1099
Size: 248 3420
NgoMIV G'CCGG_C
Cuts at: 1847 1847
Size: 3668
NspI r_CATG'y
Cuts at: 721 1221 2128 721
Size: 500 907 2261
Fragments arranged by size:
2261 907 500
PciI A'CATG_T
Cuts at: 2124 2124
Size: 3668
PmeI GTTT'AAAC
Cuts at: 667 667
Size: 3668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 3636
PstI C_TGCA'G
Cuts at: 715 1400 715
Size: 685 2983
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3668
PvuII CAG'CTG
Cuts at: 447 1093 1453 447
Size: 646 360 2662
Fragments arranged by size:
2662 646 360
RsrII CG'GwC_CG
Cuts at: 1863 1863
Size: 3668
SacI G_AGCT'C
Cuts at: 682 682
Size: 3668
SalI G'TCGA_C
Cuts at: 705 705
Size: 3668
SapI GCTCTTCn'nnn_
Cuts at: 1691 1901 1691
Size: 210 3458
SbfI CC_TGCA'GG
Cuts at: 715 715
Size: 3668
ScaI AGT'ACT
Cuts at: 3185 3185
Size: 3668
SfcI C'TryA_G
Cuts at: 33 655 711 1396 2389 2580 33
Size: 622 56 685 993 191 1121
Fragments arranged by size:
1121 993 685 622 191 56
SfoI GGC'GCC
Cuts at: 1100 1348 1100
Size: 248 3420
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
SmaI CCC'GGG
Cuts at: 690 690
Size: 3668
SmlI C'TyrA_G
Cuts at: 823 1738 2230 2492 823
Size: 915 492 262 1999
Fragments arranged by size:
1999 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3668
SphI G_CATG'C
Cuts at: 721 1221 721
Size: 500 3168
StuI AGG'CCT
Cuts at: 156 156
Size: 3668
StyI C'CwwG_G
Cuts at: 802 1156 802
Size: 354 3314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1251 1384 1843 2052 1251
Size: 133 459 209 2867
Fragments arranged by size:
2867 459 209 133
TatI w'GTAC_w
Cuts at: 3183 3183
Size: 3668
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 3636
Tth111I GACn'n_nGTC
Cuts at: 1465 1465
Size: 3668
XbaI T'CTAG_A
Cuts at: 699 699
Size: 3668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1163 1163
Size: 3668
XmaI C'CCGG_G
Cuts at: 688 688
Size: 3668
XmnI GAAnn'nnTTC
Cuts at: 185 185
Size: 3668
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI