Restriction map for
pF1KB6818
(Circular) (Six-base) MAPSORT of: pF1KB6818.seq Check: 7698 from: 1 to: 3671
pF1KB6818 3671 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 709 709
Size: 3671
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 3511 3511
Size: 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1496 1928 2675 2943 1496
Size: 432 747 268 2224
Fragments arranged by size:
2224 747 432 268
AflIII A'CryG_T
Cuts at: 1023 2127 3104 1023
Size: 1104 977 1590
Fragments arranged by size:
1590 1104 977
AgeI A'CCGG_T
Cuts at: 2059 2059
Size: 3671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 289 289
Size: 3671
AlwNI CAG_nnn'CTG
Cuts at: 976 2543 976
Size: 1567 2104
ApaLI G'TGCA_C
Cuts at: 2441 2441
Size: 3671
ApoI r'AATT_y
Cuts at: 583 675 3024 3280 583
Size: 92 2349 256 974
Fragments arranged by size:
2349 974 256 92
AseI AT'TA_AT
Cuts at: 20 20
Size: 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 139 691 139
Size: 552 3119
BamHI G'GATC_C
Cuts at: 696 696
Size: 3671
BanI G'GyrC_C
Cuts at: 687 1101 1349 1384 687
Size: 414 248 35 2974
Fragments arranged by size:
2974 414 248 35
BanII G_rGCy'C
Cuts at: 685 1715 685
Size: 1030 2641
BbeI G_GCGC'C
Cuts at: 1105 1353 1105
Size: 248 3423
BbsI GAAGACnn'nnnn_
Cuts at: 316 316
Size: 3671
BciVI GTATCCnnnnn_n'
Cuts at: 436 1569 2330 3671 436
Size: 1133 761 1341 436
Fragments arranged by size:
1341 1133 761 436
BclI T'GATC_A
Cuts at: 1191 1191
Size: 3671
BglI GCCn_nnn'nGGC
Cuts at: 346 346
Size: 3671
BglII A'GATC_T
Cuts at: 1186 3223 1186
Size: 2037 1634
BlpI GC'TnA_GC
Cuts at: 783 783
Size: 3671
Bme1580I G_kGCm'C
Cuts at: 1296 1389 2445 1296
Size: 93 1056 2522
Fragments arranged by size:
2522 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 883 964 1299 3443 883
Size: 81 335 2144 1111
Fragments arranged by size:
2144 1111 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 3671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 220
Size: 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1726 2218 2516 847
Size: 879 492 298 2002
Fragments arranged by size:
2002 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3375 3375
Size: 3671
BsaAI yAC'GTr
Cuts at: 1024 1024
Size: 3671
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 1102 1350 2052 3548 1102
Size: 248 702 1496 1225
Fragments arranged by size:
1496 1225 702 248
BsaWI w'CCGG_w
Cuts at: 860 1381 2059 2333 2480 2891 860
Size: 521 678 274 147 411 1640
Fragments arranged by size:
1640 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 182 1096 1854 2431 2927 182
Size: 914 758 577 496 926
Fragments arranged by size:
926 914 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 520 280
Size: 240 3431
BsiEI CG_ry'CG
Cuts at: 104 1213 1259 2467 104
Size: 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 46
BsiHKAI G_wGCw'C
Cuts at: 685 1463 1653 2445 685
Size: 778 190 792 1911
Fragments arranged by size:
1911 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 685 1296 1389 1463 1653 1715 2445 685
Size: 611 93 74 190 62 730 1911
Fragments arranged by size:
1911 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2891 2891
Size: 3671
BspHI T'CATG_A
Cuts at: 621 2847 621
Size: 2226 1445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 721 935 1237 1618 721
Size: 214 302 381 2774
Fragments arranged by size:
2774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1963 2017 3501 3666 50
Size: 1913 54 1484 165 55
Fragments arranged by size:
1913 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1583 1583
Size: 3671
BsrFI r'CCGG_y
Cuts at: 1669 1850 2059 1669
Size: 181 209 3281
Fragments arranged by size:
3281 209 181
BssSI C'ACGA_G
Cuts at: 257 1942 2300 257
Size: 1685 358 1628
Fragments arranged by size:
1685 1628 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1048 1048
Size: 3671
BstBI TT'CG_AA
Cuts at: 2032 2032
Size: 3671
BstXI CCAn_nnnn'nTGG
Cuts at: 334 334
Size: 3671
BstYI r'GATC_y
Cuts at: 1 696 1163 1186 1521 1767 2779 2865 2877
Size: 695 467 23 335 246 1012 86 12
Cuts at: 2877 3223 1
Size: 346 449
Fragments arranged by size:
1012 695 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 168 107
Size: 61 3610
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 327 1621 1802 1965 327
Size: 1294 181 163 2033
Fragments arranged by size:
2033 1294 181 163
BtsI GCAGTG_nn'
Cuts at: 134 469 914 134
Size: 335 445 2891
Fragments arranged by size:
2891 445 335
DraI TTT'AAA
Cuts at: 670 670
Size: 3671
DrdI GACnn_nn'nnGTC
Cuts at: 1377 2235 1377
Size: 858 2813
EaeI y'GGCC_r
Cuts at: 324 347 554 597 1256 1430 1821 1848 3193
Size: 23 207 43 659 174 391 27 1345
Cuts at: 3193 324
Size: 802
Fragments arranged by size:
1345 802 659 391 207 174 43 27
23
EagI C'GGCC_G
Cuts at: 1256 1256
Size: 3671
EarI CTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1879 2189 2335 3481 1879
Size: 310 146 1146 2069
Fragments arranged by size:
2069 1146 310 146
EcoICRI GAG'CTC
Cuts at: 683 683
Size: 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 1496 1928 2675 2943 220
Size: 1276 432 747 268 948
Fragments arranged by size:
1276 948 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 810 810
Size: 3671
EcoRI G'AATT_C
Cuts at: 675 675
Size: 3671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1690 1690
Size: 3671
FspI TGC'GCA
Cuts at: 1452 1452
Size: 3671
HaeII r_GCGC'y
Cuts at: 1105 1353 2375 3354 1105
Size: 248 1022 979 1422
Fragments arranged by size:
1422 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 251 283 1513 1543 1545 1575 251
Size: 32 1230 30 2 30 2347
Fragments arranged by size:
2347 1230 32 30 30 2
HincII GTy'rAC
Cuts at: 710 710
Size: 3671
KasI G'GCGC_C
Cuts at: 1101 1349 1101
Size: 248 3423
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 545 2342 2526 545
Size: 1797 184 1690
Fragments arranged by size:
1797 1690 184
MscI TGG'CCA
Cuts at: 326 599 1432 326
Size: 273 833 2565
Fragments arranged by size:
2565 833 273
MslI CAynn'nnrTG
Cuts at: 302 1660 1787 302
Size: 1358 127 2186
Fragments arranged by size:
2186 1358 127
MspA1I CmG'CkG
Cuts at: 782 1096 1456 2469 2714 782
Size: 314 360 1013 245 1739
Fragments arranged by size:
1739 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1852 1852
Size: 3671
NarI GG'CG_CC
Cuts at: 1102 1350 1102
Size: 248 3423
NcoI C'CATG_G
Cuts at: 107 107
Size: 3671
NgoMIV G'CCGG_C
Cuts at: 1850 1850
Size: 3671
NspI r_CATG'y
Cuts at: 240 724 1224 2131 240
Size: 484 500 907 1780
Fragments arranged by size:
1780 907 500 484
PciI A'CATG_T
Cuts at: 2127 2127
Size: 3671
PflMI CCAn_nnn'nTGG
Cuts at: 225 225
Size: 3671
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 3639
PstI C_TGCA'G
Cuts at: 718 1403 718
Size: 685 2986
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 1096 1456 1096
Size: 360 3311
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 685 685
Size: 3671
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 3188 3188
Size: 3671
SexAI A'CCwGG_T
Cuts at: 210 210
Size: 3671
SfcI C'TryA_G
Cuts at: 33 714 1399 2392 2583 33
Size: 681 685 993 191 1121
Fragments arranged by size:
1121 993 685 681 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 346 346
Size: 3671
SfoI GGC'GCC
Cuts at: 1103 1351 1103
Size: 248 3423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 141 693 141
Size: 552 3119
SmlI C'TyrA_G
Cuts at: 826 1741 2233 2495 826
Size: 915 492 262 2002
Fragments arranged by size:
2002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 724 1224 724
Size: 500 3171
StyI C'CwwG_G
Cuts at: 107 805 1159 107
Size: 698 354 2619
Fragments arranged by size:
2619 698 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 174 181 1254 1387 1846 2055 174
Size: 7 1073 133 459 209 1790
Fragments arranged by size:
1790 1073 459 209 133 7
TatI w'GTAC_w
Cuts at: 3186 3186
Size: 3671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 3639
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 702 702
Size: 3671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 175 1166 175
Size: 991 2680
XmaI C'CCGG_G
Cuts at: 139 691 139
Size: 552 3119
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI