Restriction map for
pF1KB6819
(Circular) (Six-base) MAPSORT of: pF1KB6819.seq Check: 6973 from: 1 to: 3599
pF1KB6819 3599 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 637 637
Size: 3599
Acc65I G'GTAC_C
Cuts at: 615 615
Size: 3599
AclI AA'CG_TT
Cuts at: 3439 3439
Size: 3599
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 1424 1856 2603 2871 209
Size: 1215 432 747 268 937
Fragments arranged by size:
1215 937 747 432 268
AflIII A'CryG_T
Cuts at: 951 2055 3032 951
Size: 1104 977 1518
Fragments arranged by size:
1518 1104 977
AgeI A'CCGG_T
Cuts at: 1987 1987
Size: 3599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 332 390 332
Size: 58 3541
AlwNI CAG_nnn'CTG
Cuts at: 499 904 2471 499
Size: 405 1567 1627
Fragments arranged by size:
1627 1567 405
ApaLI G'TGCA_C
Cuts at: 2369 2369
Size: 3599
ApoI r'AATT_y
Cuts at: 113 544 603 2952 3208 113
Size: 431 59 2349 256 504
Fragments arranged by size:
2349 504 431 256 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 3599
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3599
AvaI C'yCGr_G
Cuts at: 619 619
Size: 3599
BamHI G'GATC_C
Cuts at: 624 624
Size: 3599
BanI G'GyrC_C
Cuts at: 615 1029 1277 1312 615
Size: 414 248 35 2902
Fragments arranged by size:
2902 414 248 35
BanII G_rGCy'C
Cuts at: 613 1643 613
Size: 1030 2569
BbeI G_GCGC'C
Cuts at: 1033 1281 1033
Size: 248 3351
BbsI GAAGACnn'nnnn_
Cuts at: 198 467 198
Size: 269 3330
BciVI GTATCCnnnnn_n'
Cuts at: 1497 2258 3599 1497
Size: 761 1341 1497
Fragments arranged by size:
1497 1341 761
BclI T'GATC_A
Cuts at: 449 1119 449
Size: 670 2929
BfrBI ATG'CAT
Cuts at: 309 309
Size: 3599
BglII A'GATC_T
Cuts at: 291 1114 3151 291
Size: 823 2037 739
Fragments arranged by size:
2037 823 739
BlpI GC'TnA_GC
Cuts at: 711 711
Size: 3599
Bme1580I G_kGCm'C
Cuts at: 1224 1317 2373 1224
Size: 93 1056 2450
Fragments arranged by size:
2450 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 264 344 811 892 1227 3371 264
Size: 80 467 81 335 2144 492
Fragments arranged by size:
2144 492 467 335 81 80
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1966 1966
Size: 3599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 544
Size: 3599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 775 1654 2146 2444 427
Size: 348 879 492 298 1582
Fragments arranged by size:
1582 879 492 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 3303 3303
Size: 3599
BsaAI yAC'GTr
Cuts at: 952 952
Size: 3599
BsaBI GATnn'nnATC
Cuts at: 6 1113 3150 6
Size: 1107 2037 455
Fragments arranged by size:
2037 1107 455
BsaHI Gr'CG_yC
Cuts at: 1030 1278 1980 3476 1030
Size: 248 702 1496 1153
Fragments arranged by size:
1496 1153 702 248
BsaWI w'CCGG_w
Cuts at: 412 788 1309 1987 2261 2408 2819 412
Size: 376 521 678 274 147 411 1192
Fragments arranged by size:
1192 678 521 411 376 274 147
BseYI C'CCAG_C
Cuts at: 1024 1782 2359 2855 1024
Size: 758 577 496 1768
Fragments arranged by size:
1768 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 248
Size: 3599
BsiEI CG_ry'CG
Cuts at: 104 1141 1187 2395 104
Size: 1037 46 1208 1308
Fragments arranged by size:
1308 1208 1037 46
BsiHKAI G_wGCw'C
Cuts at: 613 1391 1581 2373 613
Size: 778 190 792 1839
Fragments arranged by size:
1839 792 778 190
BsmI GAATG_Cn'
Cuts at: 197 197
Size: 3599
Bsp1286I G_dGCh'C
Cuts at: 613 1224 1317 1391 1581 1643 2373 613
Size: 611 93 74 190 62 730 1839
Fragments arranged by size:
1839 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 412 2819 412
Size: 2407 1192
BspHI T'CATG_A
Cuts at: 429 2775 429
Size: 2346 1253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 649 863 1165 1546 649
Size: 214 302 381 2702
Fragments arranged by size:
2702 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1891 1945 3429 3594 50
Size: 1841 54 1484 165 55
Fragments arranged by size:
1841 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 374 1511 374
Size: 1137 2462
BsrFI r'CCGG_y
Cuts at: 1597 1778 1987 1597
Size: 181 209 3209
Fragments arranged by size:
3209 209 181
BsrGI T'GTAC_A
Cuts at: 561 561
Size: 3599
BssSI C'ACGA_G
Cuts at: 1870 2228 1870
Size: 358 3241
BstAPI GCAn_nnn'nTGC
Cuts at: 976 976
Size: 3599
BstBI TT'CG_AA
Cuts at: 118 1960 118
Size: 1842 1757
BstYI r'GATC_y
Cuts at: 1 291 624 1091 1114 1449 1695 2707 2793
Size: 290 333 467 23 335 246 1012 86
Cuts at: 2793 2805 3151 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 333 290 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1549 1730 1893 1549
Size: 181 163 3255
Fragments arranged by size:
3255 181 163
BtsI GCAGTG_nn'
Cuts at: 842 842
Size: 3599
DraI TTT'AAA
Cuts at: 598 598
Size: 3599
DrdI GACnn_nn'nnGTC
Cuts at: 1305 2163 1305
Size: 858 2741
EaeI y'GGCC_r
Cuts at: 1184 1358 1749 1776 3121 1184
Size: 174 391 27 1345 1662
Fragments arranged by size:
1662 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1184 1184
Size: 3599
EarI CTCTTCn'nnn_
Cuts at: 1622 1832 1622
Size: 210 3389
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1807 2117 2263 3409 1807
Size: 310 146 1146 1997
Fragments arranged by size:
1997 1146 310 146
EcoICRI GAG'CTC
Cuts at: 611 611
Size: 3599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 544 1424 1856 2603 2871 209
Size: 335 880 432 747 268 937
Fragments arranged by size:
937 880 747 432 335 268
EcoO109I rG'GnC_Cy
Cuts at: 738 738
Size: 3599
EcoRI G'AATT_C
Cuts at: 603 603
Size: 3599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1618 1618
Size: 3599
FspI TGC'GCA
Cuts at: 1380 1380
Size: 3599
HaeII r_GCGC'y
Cuts at: 1033 1281 2303 3282 1033
Size: 248 1022 979 1350
Fragments arranged by size:
1350 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1441 1471 1473 1503 1441
Size: 30 2 30 3537
Fragments arranged by size:
3537 30 30 2
HincII GTy'rAC
Cuts at: 638 638
Size: 3599
KasI G'GCGC_C
Cuts at: 1029 1277 1029
Size: 248 3351
KpnI G_GTAC'C
Cuts at: 619 619
Size: 3599
MluI A'CGCG_T
Cuts at: 3032 3032
Size: 3599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2270 2454 2270
Size: 184 3415
MscI TGG'CCA
Cuts at: 1360 1360
Size: 3599
MslI CAynn'nnrTG
Cuts at: 314 1588 1715 314
Size: 1274 127 2198
Fragments arranged by size:
2198 1274 127
MspA1I CmG'CkG
Cuts at: 710 1024 1384 2397 2642 710
Size: 314 360 1013 245 1667
Fragments arranged by size:
1667 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1780 1780
Size: 3599
NarI GG'CG_CC
Cuts at: 1030 1278 1030
Size: 248 3351
NgoMIV G'CCGG_C
Cuts at: 1778 1778
Size: 3599
NsiI A_TGCA'T
Cuts at: 311 311
Size: 3599
NspI r_CATG'y
Cuts at: 652 1152 2059 652
Size: 500 907 2192
Fragments arranged by size:
2192 907 500
PciI A'CATG_T
Cuts at: 2055 2055
Size: 3599
PmeI GTTT'AAAC
Cuts at: 598 598
Size: 3599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2770 2738
Size: 32 3567
PstI C_TGCA'G
Cuts at: 646 1331 646
Size: 685 2914
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3599
PvuII CAG'CTG
Cuts at: 1024 1384 1024
Size: 360 3239
RsrII CG'GwC_CG
Cuts at: 1794 1794
Size: 3599
SacI G_AGCT'C
Cuts at: 613 613
Size: 3599
SalI G'TCGA_C
Cuts at: 636 636
Size: 3599
SapI GCTCTTCn'nnn_
Cuts at: 1622 1832 1622
Size: 210 3389
SbfI CC_TGCA'GG
Cuts at: 646 646
Size: 3599
ScaI AGT'ACT
Cuts at: 3116 3116
Size: 3599
SfcI C'TryA_G
Cuts at: 33 642 1327 2320 2511 33
Size: 609 685 993 191 1121
Fragments arranged by size:
1121 993 685 609 191
SfoI GGC'GCC
Cuts at: 1031 1279 1031
Size: 248 3351
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3599
SmaI CCC'GGG
Cuts at: 621 621
Size: 3599
SmlI C'TyrA_G
Cuts at: 406 754 1669 2161 2423 406
Size: 348 915 492 262 1582
Fragments arranged by size:
1582 915 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3599
SphI G_CATG'C
Cuts at: 652 1152 652
Size: 500 3099
SspI AAT'ATT
Cuts at: 537 537
Size: 3599
StyI C'CwwG_G
Cuts at: 733 1087 733
Size: 354 3245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 246 1182 1315 1774 1983 246
Size: 936 133 459 209 1862
Fragments arranged by size:
1862 936 459 209 133
TatI w'GTAC_w
Cuts at: 561 3114 561
Size: 2553 1046
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3567
Tth111I GACn'n_nGTC
Cuts at: 1396 1396
Size: 3599
XbaI T'CTAG_A
Cuts at: 630 630
Size: 3599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1094 1094
Size: 3599
XmaI C'CCGG_G
Cuts at: 619 619
Size: 3599
XmnI GAAnn'nnTTC
Cuts at: 138 138
Size: 3599
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI