Restriction map for
pF1KB6820
(Circular) (Six-base) MAPSORT of: pF1KB6820.seq Check: 6535 from: 1 to: 3671
pF1KB6820 3671 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 709 709
Size: 3671
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 3511 3511
Size: 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1496 1928 2675 2943 613
Size: 883 432 747 268 1341
Fragments arranged by size:
1341 883 747 432 268
AflIII A'CryG_T
Cuts at: 1023 2127 3104 1023
Size: 1104 977 1590
Fragments arranged by size:
1590 1104 977
AgeI A'CCGG_T
Cuts at: 2059 2059
Size: 3671
AleI CACnn'nnGTG
Cuts at: 630 630
Size: 3671
AlwNI CAG_nnn'CTG
Cuts at: 976 2543 976
Size: 1567 2104
ApaLI G'TGCA_C
Cuts at: 2441 2441
Size: 3671
ApoI r'AATT_y
Cuts at: 473 575 675 3024 3280 473
Size: 102 100 2349 256 864
Fragments arranged by size:
2349 864 256 102 100
AseI AT'TA_AT
Cuts at: 20 20
Size: 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 198 691 198
Size: 493 3178
BamHI G'GATC_C
Cuts at: 696 696
Size: 3671
BanI G'GyrC_C
Cuts at: 687 1101 1349 1384 687
Size: 414 248 35 2974
Fragments arranged by size:
2974 414 248 35
BanII G_rGCy'C
Cuts at: 685 1715 685
Size: 1030 2641
BbeI G_GCGC'C
Cuts at: 1105 1353 1105
Size: 248 3423
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3637
BciVI GTATCCnnnnn_n'
Cuts at: 1569 2330 3671 1569
Size: 761 1341 1569
Fragments arranged by size:
1569 1341 761
BclI T'GATC_A
Cuts at: 1191 1191
Size: 3671
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3671
BglII A'GATC_T
Cuts at: 311 504 1186 3223 311
Size: 193 682 2037 759
Fragments arranged by size:
2037 759 682 193
BlpI GC'TnA_GC
Cuts at: 783 783
Size: 3671
Bme1580I G_kGCm'C
Cuts at: 1296 1389 2445 1296
Size: 93 1056 2522
Fragments arranged by size:
2522 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 883 964 1299 3443 883
Size: 81 335 2144 1111
Fragments arranged by size:
2144 1111 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1726 2218 2516 847
Size: 879 492 298 2002
Fragments arranged by size:
2002 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3375 3375
Size: 3671
BsaAI yAC'GTr
Cuts at: 1024 1024
Size: 3671
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 1102 1350 2052 3548 1102
Size: 248 702 1496 1225
Fragments arranged by size:
1496 1225 702 248
BsaWI w'CCGG_w
Cuts at: 860 1381 2059 2333 2480 2891 860
Size: 521 678 274 147 411 1640
Fragments arranged by size:
1640 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 383 383
Size: 3671
BseYI C'CCAG_C
Cuts at: 1096 1854 2431 2927 1096
Size: 758 577 496 1840
Fragments arranged by size:
1840 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1213 1259 2467 104
Size: 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 46
BsiHKAI G_wGCw'C
Cuts at: 685 1463 1653 2445 685
Size: 778 190 792 1911
Fragments arranged by size:
1911 792 778 190
BsmI GAATG_Cn'
Cuts at: 645 645
Size: 3671
Bsp1286I G_dGCh'C
Cuts at: 685 1296 1389 1463 1653 1715 2445 685
Size: 611 93 74 190 62 730 1911
Fragments arranged by size:
1911 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2891 2891
Size: 3671
BspHI T'CATG_A
Cuts at: 2847 2847
Size: 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 721 935 1237 1618 721
Size: 214 302 381 2774
Fragments arranged by size:
2774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 357 1963 2017 3501 3666 50
Size: 307 1606 54 1484 165 55
Fragments arranged by size:
1606 1484 307 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1583 1583
Size: 3671
BsrFI r'CCGG_y
Cuts at: 1669 1850 2059 1669
Size: 181 209 3281
Fragments arranged by size:
3281 209 181
BssSI C'ACGA_G
Cuts at: 181 1942 2300 181
Size: 1761 358 1552
Fragments arranged by size:
1761 1552 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1048 1048
Size: 3671
BstBI TT'CG_AA
Cuts at: 342 2032 342
Size: 1690 1981
BstYI r'GATC_y
Cuts at: 1 311 504 696 1163 1186 1521 1767 2779
Size: 310 193 192 467 23 335 246 1012
Cuts at: 2779 2865 2877 3223 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 310 246 193
192 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 210 1621 1802 1965 210
Size: 1411 181 163 1916
Fragments arranged by size:
1916 1411 181 163
BtsI GCAGTG_nn'
Cuts at: 914 914
Size: 3671
DraI TTT'AAA
Cuts at: 670 670
Size: 3671
DrdI GACnn_nn'nnGTC
Cuts at: 1377 2235 1377
Size: 858 2813
EaeI y'GGCC_r
Cuts at: 1256 1430 1821 1848 3193 1256
Size: 174 391 27 1345 1734
Fragments arranged by size:
1734 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1256 1256
Size: 3671
EarI CTCTTCn'nnn_
Cuts at: 157 158 1694 1904 157
Size: 1 1536 210 1924
Fragments arranged by size:
1924 1536 210 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1879 2189 2335 3481 1879
Size: 310 146 1146 2069
Fragments arranged by size:
2069 1146 310 146
EcoICRI GAG'CTC
Cuts at: 683 683
Size: 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1496 1928 2675 2943 613
Size: 883 432 747 268 1341
Fragments arranged by size:
1341 883 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 810 810
Size: 3671
EcoRI G'AATT_C
Cuts at: 473 675 473
Size: 202 3469
EcoRV GAT'ATC
Cuts at: 621 621
Size: 3671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 228 1690 228
Size: 1462 2209
FspI TGC'GCA
Cuts at: 1452 1452
Size: 3671
HaeII r_GCGC'y
Cuts at: 118 1105 1353 2375 3354 118
Size: 987 248 1022 979 435
Fragments arranged by size:
1022 987 979 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1513 1543 1545 1575 1513
Size: 30 2 30 3609
Fragments arranged by size:
3609 30 30 2
HincII GTy'rAC
Cuts at: 710 710
Size: 3671
HindIII A'AGCT_T
Cuts at: 422 422
Size: 3671
KasI G'GCGC_C
Cuts at: 1101 1349 1101
Size: 248 3423
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2526 2342
Size: 184 3487
MscI TGG'CCA
Cuts at: 1432 1432
Size: 3671
MslI CAynn'nnrTG
Cuts at: 630 1660 1787 630
Size: 1030 127 2514
Fragments arranged by size:
2514 1030 127
MspA1I CmG'CkG
Cuts at: 782 1096 1456 2469 2714 782
Size: 314 360 1013 245 1739
Fragments arranged by size:
1739 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1852 1852
Size: 3671
NarI GG'CG_CC
Cuts at: 1102 1350 1102
Size: 248 3423
NgoMIV G'CCGG_C
Cuts at: 1850 1850
Size: 3671
NspI r_CATG'y
Cuts at: 351 724 1224 2131 351
Size: 373 500 907 1891
Fragments arranged by size:
1891 907 500 373
PciI A'CATG_T
Cuts at: 2127 2127
Size: 3671
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 3639
PstI C_TGCA'G
Cuts at: 194 405 718 1403 194
Size: 211 313 685 2462
Fragments arranged by size:
2462 685 313 211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 1096 1456 1096
Size: 360 3311
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 685 685
Size: 3671
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 157 1694 1904 157
Size: 1537 210 1924
Fragments arranged by size:
1924 1537 210
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 196 3188 196
Size: 2992 679
SfcI C'TryA_G
Cuts at: 33 190 401 714 1399 2392 2583 33
Size: 157 211 313 685 993 191 1121
Fragments arranged by size:
1121 993 685 313 211 191 157
SfoI GGC'GCC
Cuts at: 1103 1351 1103
Size: 248 3423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 693 693
Size: 3671
SmlI C'TyrA_G
Cuts at: 826 1741 2233 2495 826
Size: 915 492 262 2002
Fragments arranged by size:
2002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 724 1224 724
Size: 500 3171
StyI C'CwwG_G
Cuts at: 518 805 1159 518
Size: 287 354 3030
Fragments arranged by size:
3030 354 287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1254 1387 1846 2055 1254
Size: 133 459 209 2870
Fragments arranged by size:
2870 459 209 133
TatI w'GTAC_w
Cuts at: 194 3186 194
Size: 2992 679
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 464 496 3188 3220 464
Size: 32 2692 32 915
Fragments arranged by size:
2692 915 32 32
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 450 655 702 450
Size: 205 47 3419
Fragments arranged by size:
3419 205 47
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1166 1166
Size: 3671
XmaI C'CCGG_G
Cuts at: 691 691
Size: 3671
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI