Restriction map for
pF1KB6821
(Circular) (Six-base) MAPSORT of: pF1KB6821.seq Check: 3445 from: 1 to: 3602
pF1KB6821 3602 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 640 640
Size: 3602
Acc65I G'GTAC_C
Cuts at: 618 618
Size: 3602
AclI AA'CG_TT
Cuts at: 3442 3442
Size: 3602
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1427 1859 2606 2874 1427
Size: 432 747 268 2155
Fragments arranged by size:
2155 747 432 268
AflIII A'CryG_T
Cuts at: 954 2058 3035 954
Size: 1104 977 1521
Fragments arranged by size:
1521 1104 977
AgeI A'CCGG_T
Cuts at: 1990 1990
Size: 3602
AlwNI CAG_nnn'CTG
Cuts at: 907 2474 907
Size: 1567 2035
ApaI G_GGCC'C
Cuts at: 143 143
Size: 3602
ApaLI G'TGCA_C
Cuts at: 2372 2372
Size: 3602
ApoI r'AATT_y
Cuts at: 316 424 606 2955 3211 316
Size: 108 182 2349 256 707
Fragments arranged by size:
2349 707 256 182 108
AseI AT'TA_AT
Cuts at: 20 20
Size: 3602
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3602
AvaI C'yCGr_G
Cuts at: 622 622
Size: 3602
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 547 580 547
Size: 33 3569
BamHI G'GATC_C
Cuts at: 627 627
Size: 3602
BanI G'GyrC_C
Cuts at: 618 1032 1280 1315 618
Size: 414 248 35 2905
Fragments arranged by size:
2905 414 248 35
BanII G_rGCy'C
Cuts at: 143 516 616 1646 143
Size: 373 100 1030 2099
Fragments arranged by size:
2099 1030 373 100
BbeI G_GCGC'C
Cuts at: 1036 1284 1036
Size: 248 3354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 150 184 90
Size: 34 26 34 3508
Fragments arranged by size:
3508 34 34 26
BciVI GTATCCnnnnn_n'
Cuts at: 1500 2261 3602 1500
Size: 761 1341 1500
Fragments arranged by size:
1500 1341 761
BclI T'GATC_A
Cuts at: 1122 1122
Size: 3602
BglII A'GATC_T
Cuts at: 1117 3154 1117
Size: 2037 1565
BlpI GC'TnA_GC
Cuts at: 714 714
Size: 3602
Bme1580I G_kGCm'C
Cuts at: 143 1227 1320 2376 143
Size: 1084 93 1056 1369
Fragments arranged by size:
1369 1084 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 814 895 1230 3374 814
Size: 81 335 2144 1042
Fragments arranged by size:
2144 1042 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1969 1969
Size: 3602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1657 2149 2447 778
Size: 879 492 298 1933
Fragments arranged by size:
1933 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3306 3306
Size: 3602
BsaAI yAC'GTr
Cuts at: 955 955
Size: 3602
BsaBI GATnn'nnATC
Cuts at: 6 1116 3153 6
Size: 1110 2037 455
Fragments arranged by size:
2037 1110 455
BsaHI Gr'CG_yC
Cuts at: 1033 1281 1983 3479 1033
Size: 248 702 1496 1156
Fragments arranged by size:
1496 1156 702 248
BsaWI w'CCGG_w
Cuts at: 272 791 1312 1990 2264 2411 2822 272
Size: 519 521 678 274 147 411 1052
Fragments arranged by size:
1052 678 521 519 411 274 147
BseYI C'CCAG_C
Cuts at: 1027 1785 2362 2858 1027
Size: 758 577 496 1771
Fragments arranged by size:
1771 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1144 1190 2398 104
Size: 1040 46 1208 1308
Fragments arranged by size:
1308 1208 1040 46
BsiHKAI G_wGCw'C
Cuts at: 616 1394 1584 2376 616
Size: 778 190 792 1842
Fragments arranged by size:
1842 792 778 190
BsmI GAATG_Cn'
Cuts at: 268 268
Size: 3602
Bsp1286I G_dGCh'C
Cuts at: 143 516 616 1227 1320 1394 1584 1646 2376
Size: 373 100 611 93 74 190 62 730
Cuts at: 2376 143
Size: 1369
Fragments arranged by size:
1369 730 611 373 190 100 93 74
62
BspEI T'CCGG_A
Cuts at: 272 2822 272
Size: 2550 1052
BspHI T'CATG_A
Cuts at: 2778 2778
Size: 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 652 866 1168 1549 652
Size: 214 302 381 2705
Fragments arranged by size:
2705 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1894 1948 3432 3597 50
Size: 1844 54 1484 165 55
Fragments arranged by size:
1844 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1514 1514
Size: 3602
BsrFI r'CCGG_y
Cuts at: 1600 1781 1990 1600
Size: 181 209 3212
Fragments arranged by size:
3212 209 181
BssSI C'ACGA_G
Cuts at: 1873 2231 1873
Size: 358 3244
BstAPI GCAn_nnn'nTGC
Cuts at: 182 979 182
Size: 797 2805
BstBI TT'CG_AA
Cuts at: 1963 1963
Size: 3602
BstYI r'GATC_y
Cuts at: 1 627 1094 1117 1452 1698 2710 2796 2808
Size: 626 467 23 335 246 1012 86 12
Cuts at: 2808 3154 1
Size: 346 449
Fragments arranged by size:
1012 626 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 397 1552 1733 1896 397
Size: 1155 181 163 2103
Fragments arranged by size:
2103 1155 181 163
BtsI GCAGTG_nn'
Cuts at: 845 845
Size: 3602
DraI TTT'AAA
Cuts at: 228 601 228
Size: 373 3229
DrdI GACnn_nn'nnGTC
Cuts at: 1308 2166 1308
Size: 858 2744
EaeI y'GGCC_r
Cuts at: 1187 1361 1752 1779 3124 1187
Size: 174 391 27 1345 1665
Fragments arranged by size:
1665 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1187 1187
Size: 3602
EarI CTCTTCn'nnn_
Cuts at: 382 539 1625 1835 382
Size: 157 1086 210 2149
Fragments arranged by size:
2149 1086 210 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1810 2120 2266 3412 1810
Size: 310 146 1146 2000
Fragments arranged by size:
2000 1146 310 146
EcoICRI GAG'CTC
Cuts at: 614 614
Size: 3602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1427 1859 2606 2874 1427
Size: 432 747 268 2155
Fragments arranged by size:
2155 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 521 521
Size: 3602
EcoO109I rG'GnC_Cy
Cuts at: 139 741 139
Size: 602 3000
EcoRI G'AATT_C
Cuts at: 606 606
Size: 3602
EcoRV GAT'ATC
Cuts at: 477 477
Size: 3602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1621 1621
Size: 3602
FspI TGC'GCA
Cuts at: 1383 1383
Size: 3602
HaeII r_GCGC'y
Cuts at: 417 1036 1284 2306 3285 417
Size: 619 248 1022 979 734
Fragments arranged by size:
1022 979 734 619 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1444 1474 1476 1506 1444
Size: 30 2 30 3540
Fragments arranged by size:
3540 30 30 2
HincII GTy'rAC
Cuts at: 641 641
Size: 3602
KasI G'GCGC_C
Cuts at: 1032 1280 1032
Size: 248 3354
KpnI G_GTAC'C
Cuts at: 622 622
Size: 3602
MluI A'CGCG_T
Cuts at: 3035 3035
Size: 3602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 521 2273 2457 521
Size: 1752 184 1666
Fragments arranged by size:
1752 1666 184
MscI TGG'CCA
Cuts at: 1363 1363
Size: 3602
MslI CAynn'nnrTG
Cuts at: 1591 1718 1591
Size: 127 3475
MspA1I CmG'CkG
Cuts at: 283 713 1027 1387 2400 2645 283
Size: 430 314 360 1013 245 1240
Fragments arranged by size:
1240 1013 430 360 314 245
NaeI GCC'GGC
Cuts at: 1783 1783
Size: 3602
NarI GG'CG_CC
Cuts at: 1033 1281 1033
Size: 248 3354
NgoMIV G'CCGG_C
Cuts at: 1781 1781
Size: 3602
NspI r_CATG'y
Cuts at: 655 1155 2062 655
Size: 500 907 2195
Fragments arranged by size:
2195 907 500
PciI A'CATG_T
Cuts at: 2058 2058
Size: 3602
PfoI T'CCnGG_A
Cuts at: 150 150
Size: 3602
PmeI GTTT'AAAC
Cuts at: 601 601
Size: 3602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 3570
PsiI TTA'TAA
Cuts at: 430 430
Size: 3602
PspOMI G'GGCC_C
Cuts at: 139 139
Size: 3602
PstI C_TGCA'G
Cuts at: 649 1334 649
Size: 685 2917
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3602
PvuII CAG'CTG
Cuts at: 283 1027 1387 283
Size: 744 360 2498
Fragments arranged by size:
2498 744 360
RsrII CG'GwC_CG
Cuts at: 1797 1797
Size: 3602
SacI G_AGCT'C
Cuts at: 616 616
Size: 3602
SalI G'TCGA_C
Cuts at: 639 639
Size: 3602
SapI GCTCTTCn'nnn_
Cuts at: 1625 1835 1625
Size: 210 3392
SbfI CC_TGCA'GG
Cuts at: 649 649
Size: 3602
ScaI AGT'ACT
Cuts at: 3119 3119
Size: 3602
SfcI C'TryA_G
Cuts at: 33 460 645 1330 2323 2514 33
Size: 427 185 685 993 191 1121
Fragments arranged by size:
1121 993 685 427 191 185
SfoI GGC'GCC
Cuts at: 1034 1282 1034
Size: 248 3354
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3602
SmaI CCC'GGG
Cuts at: 624 624
Size: 3602
SmlI C'TyrA_G
Cuts at: 757 1672 2164 2426 757
Size: 915 492 262 1933
Fragments arranged by size:
1933 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3602
SphI G_CATG'C
Cuts at: 655 1155 655
Size: 500 3102
StyI C'CwwG_G
Cuts at: 736 1090 736
Size: 354 3248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1185 1318 1777 1986 1185
Size: 133 459 209 2801
Fragments arranged by size:
2801 459 209 133
TatI w'GTAC_w
Cuts at: 3117 3117
Size: 3602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 3570
Tth111I GACn'n_nGTC
Cuts at: 1399 1399
Size: 3602
XbaI T'CTAG_A
Cuts at: 633 633
Size: 3602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1097 1097
Size: 3602
XmaI C'CCGG_G
Cuts at: 622 622
Size: 3602
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbsI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI