Restriction map for
pF1KB6822
(Circular) (Six-base) MAPSORT of: pF1KB6822.seq Check: 9797 from: 1 to: 3671
pF1KB6822 3671 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 219 405 219
Size: 186 3485
AccI GT'mk_AC
Cuts at: 709 709
Size: 3671
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 3511 3511
Size: 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1496 1928 2675 2943 1496
Size: 432 747 268 2224
Fragments arranged by size:
2224 747 432 268
AfeI AGC'GCT
Cuts at: 163 490 163
Size: 327 3344
AflIII A'CryG_T
Cuts at: 1023 2127 3104 1023
Size: 1104 977 1590
Fragments arranged by size:
1590 1104 977
AgeI A'CCGG_T
Cuts at: 2059 2059
Size: 3671
AleI CACnn'nnGTG
Cuts at: 646 646
Size: 3671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 437 437
Size: 3671
AlwNI CAG_nnn'CTG
Cuts at: 976 2543 976
Size: 1567 2104
ApaLI G'TGCA_C
Cuts at: 2441 2441
Size: 3671
ApoI r'AATT_y
Cuts at: 675 3024 3280 675
Size: 2349 256 1066
Fragments arranged by size:
2349 1066 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 143 691 143
Size: 548 3123
BamHI G'GATC_C
Cuts at: 312 696 312
Size: 384 3287
BanI G'GyrC_C
Cuts at: 429 687 1101 1349 1384 429
Size: 258 414 248 35 2716
Fragments arranged by size:
2716 414 258 248 35
BanII G_rGCy'C
Cuts at: 685 1715 685
Size: 1030 2641
BbeI G_GCGC'C
Cuts at: 1105 1353 1105
Size: 248 3423
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 316 350 316
Size: 34 3637
BciVI GTATCCnnnnn_n'
Cuts at: 1569 2330 3671 1569
Size: 761 1341 1569
Fragments arranged by size:
1569 1341 761
BclI T'GATC_A
Cuts at: 299 1191 299
Size: 892 2779
BglII A'GATC_T
Cuts at: 294 1186 3223 294
Size: 892 2037 742
Fragments arranged by size:
2037 892 742
BlpI GC'TnA_GC
Cuts at: 783 783
Size: 3671
Bme1580I G_kGCm'C
Cuts at: 434 1296 1389 2445 434
Size: 862 93 1056 1660
Fragments arranged by size:
1660 1056 862 93
BmrI ACTGGGnnnn_n'
Cuts at: 883 964 1299 3443 883
Size: 81 335 2144 1111
Fragments arranged by size:
2144 1111 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 3671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 430 580 178
Size: 252 150 3269
Fragments arranged by size:
3269 252 150
Bpu10I CC'TnA_GC
Cuts at: 467 467
Size: 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1726 2218 2516 847
Size: 879 492 298 2002
Fragments arranged by size:
2002 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3375 3375
Size: 3671
BsaAI yAC'GTr
Cuts at: 1024 1024
Size: 3671
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 1102 1350 2052 3548 1102
Size: 248 702 1496 1225
Fragments arranged by size:
1496 1225 702 248
BsaWI w'CCGG_w
Cuts at: 860 1381 2059 2333 2480 2891 860
Size: 521 678 274 147 411 1640
Fragments arranged by size:
1640 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 556 676 391
Size: 165 120 3386
Fragments arranged by size:
3386 165 120
BseYI C'CCAG_C
Cuts at: 164 1096 1854 2431 2927 164
Size: 932 758 577 496 908
Fragments arranged by size:
932 908 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 625 247
Size: 378 3293
BsiEI CG_ry'CG
Cuts at: 104 330 1213 1259 2467 104
Size: 226 883 46 1208 1308
Fragments arranged by size:
1308 1208 883 226 46
BsiHKAI G_wGCw'C
Cuts at: 450 685 1463 1653 2445 450
Size: 235 778 190 792 1676
Fragments arranged by size:
1676 792 778 235 190
BsmI GAATG_Cn'
Cuts at: 426 426
Size: 3671
Bsp1286I G_dGCh'C
Cuts at: 434 450 685 1296 1389 1463 1653 1715 2445
Size: 16 235 611 93 74 190 62 730
Cuts at: 2445 434
Size: 1660
Fragments arranged by size:
1660 730 611 235 190 93 74 62
16
BspEI T'CCGG_A
Cuts at: 2891 2891
Size: 3671
BspHI T'CATG_A
Cuts at: 2847 2847
Size: 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 219 405 721 935 1237 1618 219
Size: 186 316 214 302 381 2272
Fragments arranged by size:
2272 381 316 302 214 186
BsrBI CCG'CTC
Cuts at: 50 1963 2017 3501 3666 50
Size: 1913 54 1484 165 55
Fragments arranged by size:
1913 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 332 1583 332
Size: 1251 2420
BsrFI r'CCGG_y
Cuts at: 1669 1850 2059 1669
Size: 181 209 3281
Fragments arranged by size:
3281 209 181
BssHII G'CGCG_C
Cuts at: 187 187
Size: 3671
BssSI C'ACGA_G
Cuts at: 1942 2300 1942
Size: 358 3313
BstAPI GCAn_nnn'nTGC
Cuts at: 603 1048 603
Size: 445 3226
BstBI TT'CG_AA
Cuts at: 2032 2032
Size: 3671
BstYI r'GATC_y
Cuts at: 1 294 312 500 696 1163 1186 1521 1767
Size: 293 18 188 196 467 23 335 246
Cuts at: 1767 2779 2865 2877 3223 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 293 246 196
188 86 23 18 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3671
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 1802 1965 1621
Size: 181 163 3327
Fragments arranged by size:
3327 181 163
BtsI GCAGTG_nn'
Cuts at: 613 914 613
Size: 301 3370
DraI TTT'AAA
Cuts at: 670 670
Size: 3671
DrdI GACnn_nn'nnGTC
Cuts at: 217 1377 2235 217
Size: 1160 858 1653
Fragments arranged by size:
1653 1160 858
EaeI y'GGCC_r
Cuts at: 1256 1430 1821 1848 3193 1256
Size: 174 391 27 1345 1734
Fragments arranged by size:
1734 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1256 1256
Size: 3671
EarI CTCTTCn'nnn_
Cuts at: 146 1694 1904 146
Size: 1548 210 1913
Fragments arranged by size:
1913 1548 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1879 2189 2335 3481 1879
Size: 310 146 1146 2069
Fragments arranged by size:
2069 1146 310 146
EcoICRI GAG'CTC
Cuts at: 683 683
Size: 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 430 580 1496 1928 2675 2943 178
Size: 252 150 916 432 747 268 906
Fragments arranged by size:
916 906 747 432 268 252 150
EcoNI CCTnn'n_nnAGG
Cuts at: 220 373 394 412 220
Size: 153 21 18 3479
Fragments arranged by size:
3479 153 21 18
EcoO109I rG'GnC_Cy
Cuts at: 810 810
Size: 3671
EcoRI G'AATT_C
Cuts at: 675 675
Size: 3671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1690 1690
Size: 3671
FspI TGC'GCA
Cuts at: 1452 1452
Size: 3671
HaeII r_GCGC'y
Cuts at: 165 492 1105 1353 2375 3354 165
Size: 327 613 248 1022 979 482
Fragments arranged by size:
1022 979 613 482 327 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 279 311 1513 1543 1545 1575 279
Size: 32 1202 30 2 30 2375
Fragments arranged by size:
2375 1202 32 30 30 2
HincII GTy'rAC
Cuts at: 710 710
Size: 3671
KasI G'GCGC_C
Cuts at: 1101 1349 1101
Size: 248 3423
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2526 2342
Size: 184 3487
MscI TGG'CCA
Cuts at: 1432 1432
Size: 3671
MslI CAynn'nnrTG
Cuts at: 646 1660 1787 646
Size: 1014 127 2530
Fragments arranged by size:
2530 1014 127
MspA1I CmG'CkG
Cuts at: 132 782 1096 1456 2469 2714 132
Size: 650 314 360 1013 245 1089
Fragments arranged by size:
1089 1013 650 360 314 245
NaeI GCC'GGC
Cuts at: 1852 1852
Size: 3671
NarI GG'CG_CC
Cuts at: 1102 1350 1102
Size: 248 3423
NcoI C'CATG_G
Cuts at: 107 107
Size: 3671
NgoMIV G'CCGG_C
Cuts at: 1850 1850
Size: 3671
NspI r_CATG'y
Cuts at: 475 724 1224 2131 475
Size: 249 500 907 2015
Fragments arranged by size:
2015 907 500 249
PciI A'CATG_T
Cuts at: 2127 2127
Size: 3671
PflMI CCAn_nnn'nTGG
Cuts at: 528 528
Size: 3671
PfoI T'CCnGG_A
Cuts at: 407 557 407
Size: 150 3521
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 3639
PstI C_TGCA'G
Cuts at: 488 718 1403 488
Size: 230 685 2756
Fragments arranged by size:
2756 685 230
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 1096 1456 1096
Size: 360 3311
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 685 685
Size: 3671
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 146 1694 1904 146
Size: 1548 210 1913
Fragments arranged by size:
1913 1548 210
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 3188 3188
Size: 3671
SfcI C'TryA_G
Cuts at: 33 136 484 714 1399 2392 2583 33
Size: 103 348 230 685 993 191 1121
Fragments arranged by size:
1121 993 685 348 230 191 103
SfoI GGC'GCC
Cuts at: 1103 1351 1103
Size: 248 3423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 693 693
Size: 3671
SmlI C'TyrA_G
Cuts at: 826 1741 2233 2495 826
Size: 915 492 262 2002
Fragments arranged by size:
2002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 475 724 1224 475
Size: 249 500 2922
Fragments arranged by size:
2922 500 249
StyI C'CwwG_G
Cuts at: 107 425 548 805 1159 107
Size: 318 123 257 354 2619
Fragments arranged by size:
2619 354 318 257 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 156 1254 1387 1846 2055 156
Size: 1098 133 459 209 1772
Fragments arranged by size:
1772 1098 459 209 133
TatI w'GTAC_w
Cuts at: 320 3186 320
Size: 2866 805
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 3639
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 702 702
Size: 3671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 555 1166 555
Size: 611 3060
XmaI C'CCGG_G
Cuts at: 691 691
Size: 3671
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI