Restriction map for
pF1KB6823
(Circular) (Six-base) MAPSORT of: pF1KB6823.seq Check: 7116 from: 1 to: 3602
pF1KB6823 3602 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 248 248
Size: 3602
AccI GT'mk_AC
Cuts at: 640 640
Size: 3602
Acc65I G'GTAC_C
Cuts at: 618 618
Size: 3602
AclI AA'CG_TT
Cuts at: 3442 3442
Size: 3602
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1427 1859 2606 2874 1427
Size: 432 747 268 2155
Fragments arranged by size:
2155 747 432 268
AflIII A'CryG_T
Cuts at: 954 2058 3035 954
Size: 1104 977 1521
Fragments arranged by size:
1521 1104 977
AgeI A'CCGG_T
Cuts at: 1990 1990
Size: 3602
AlwNI CAG_nnn'CTG
Cuts at: 482 907 2474 482
Size: 425 1567 1610
Fragments arranged by size:
1610 1567 425
ApaLI G'TGCA_C
Cuts at: 2372 2372
Size: 3602
ApoI r'AATT_y
Cuts at: 606 2955 3211 606
Size: 2349 256 997
Fragments arranged by size:
2349 997 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3602
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3602
AvaI C'yCGr_G
Cuts at: 593 622 593
Size: 29 3573
BamHI G'GATC_C
Cuts at: 155 627 155
Size: 472 3130
BanI G'GyrC_C
Cuts at: 618 1032 1280 1315 618
Size: 414 248 35 2905
Fragments arranged by size:
2905 414 248 35
BanII G_rGCy'C
Cuts at: 594 616 1646 594
Size: 22 1030 2550
Fragments arranged by size:
2550 1030 22
BbeI G_GCGC'C
Cuts at: 1036 1284 1036
Size: 248 3354
BciVI GTATCCnnnnn_n'
Cuts at: 1500 2261 3602 1500
Size: 761 1341 1500
Fragments arranged by size:
1500 1341 761
BclI T'GATC_A
Cuts at: 1122 1122
Size: 3602
BglII A'GATC_T
Cuts at: 1117 3154 1117
Size: 2037 1565
BlpI GC'TnA_GC
Cuts at: 714 714
Size: 3602
Bme1580I G_kGCm'C
Cuts at: 1227 1320 2376 1227
Size: 93 1056 2453
Fragments arranged by size:
2453 1056 93
BmgBI CAC'GTC
Cuts at: 439 509 439
Size: 70 3532
BmrI ACTGGGnnnn_n'
Cuts at: 167 411 814 895 1230 3374 167
Size: 244 403 81 335 2144 395
Fragments arranged by size:
2144 403 395 335 244 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1969 1969
Size: 3602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1657 2149 2447 778
Size: 879 492 298 1933
Fragments arranged by size:
1933 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3306 3306
Size: 3602
BsaAI yAC'GTr
Cuts at: 530 955 530
Size: 425 3177
BsaBI GATnn'nnATC
Cuts at: 6 381 1116 3153 6
Size: 375 735 2037 455
Fragments arranged by size:
2037 735 455 375
BsaHI Gr'CG_yC
Cuts at: 1033 1281 1983 3479 1033
Size: 248 702 1496 1156
Fragments arranged by size:
1496 1156 702 248
BsaWI w'CCGG_w
Cuts at: 791 1312 1990 2264 2411 2822 791
Size: 521 678 274 147 411 1571
Fragments arranged by size:
1571 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 581 611 581
Size: 30 3572
BseYI C'CCAG_C
Cuts at: 482 1027 1785 2362 2858 482
Size: 545 758 577 496 1226
Fragments arranged by size:
1226 758 577 545 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 518
Size: 3602
BsiEI CG_ry'CG
Cuts at: 104 1144 1190 2398 104
Size: 1040 46 1208 1308
Fragments arranged by size:
1308 1208 1040 46
BsiHKAI G_wGCw'C
Cuts at: 180 616 1394 1584 2376 180
Size: 436 778 190 792 1406
Fragments arranged by size:
1406 792 778 436 190
Bsp1286I G_dGCh'C
Cuts at: 180 594 616 1227 1320 1394 1584 1646 2376
Size: 414 22 611 93 74 190 62 730
Cuts at: 2376 180
Size: 1406
Fragments arranged by size:
1406 730 611 414 190 93 74 62
22
BspEI T'CCGG_A
Cuts at: 2822 2822
Size: 3602
BspHI T'CATG_A
Cuts at: 2778 2778
Size: 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 248 652 866 1168 1549 248
Size: 404 214 302 381 2301
Fragments arranged by size:
2301 404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1894 1948 3432 3597 50
Size: 1844 54 1484 165 55
Fragments arranged by size:
1844 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 420 1514 420
Size: 1094 2508
BsrFI r'CCGG_y
Cuts at: 1600 1781 1990 1600
Size: 181 209 3212
Fragments arranged by size:
3212 209 181
BssSI C'ACGA_G
Cuts at: 1873 2231 1873
Size: 358 3244
BstAPI GCAn_nnn'nTGC
Cuts at: 193 979 193
Size: 786 2816
BstBI TT'CG_AA
Cuts at: 1963 1963
Size: 3602
BstYI r'GATC_y
Cuts at: 1 155 552 627 1094 1117 1452 1698 2710
Size: 154 397 75 467 23 335 246 1012
Cuts at: 2710 2796 2808 3154 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 397 346 335 246 154
86 75 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3602
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1552 1733 1896 1552
Size: 181 163 3258
Fragments arranged by size:
3258 181 163
BtsI GCAGTG_nn'
Cuts at: 301 845 301
Size: 544 3058
DraI TTT'AAA
Cuts at: 601 601
Size: 3602
DraIII CAC_nnn'GTG
Cuts at: 585 585
Size: 3602
DrdI GACnn_nn'nnGTC
Cuts at: 1308 2166 1308
Size: 858 2744
EaeI y'GGCC_r
Cuts at: 110 1187 1361 1752 1779 3124 110
Size: 1077 174 391 27 1345 588
Fragments arranged by size:
1345 1077 588 391 174 27
EagI C'GGCC_G
Cuts at: 1187 1187
Size: 3602
EarI CTCTTCn'nnn_
Cuts at: 1625 1835 1625
Size: 210 3392
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1810 2120 2266 3412 1810
Size: 310 146 1146 2000
Fragments arranged by size:
2000 1146 310 146
EcoICRI GAG'CTC
Cuts at: 614 614
Size: 3602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1427 1859 2606 2874 1427
Size: 432 747 268 2155
Fragments arranged by size:
2155 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 741 741
Size: 3602
EcoRI G'AATT_C
Cuts at: 606 606
Size: 3602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1621 1621
Size: 3602
FspI TGC'GCA
Cuts at: 1383 1383
Size: 3602
HaeII r_GCGC'y
Cuts at: 1036 1284 2306 3285 1036
Size: 248 1022 979 1353
Fragments arranged by size:
1353 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1444 1474 1476 1506 1444
Size: 30 2 30 3540
Fragments arranged by size:
3540 30 30 2
HincII GTy'rAC
Cuts at: 641 641
Size: 3602
KasI G'GCGC_C
Cuts at: 1032 1280 1032
Size: 248 3354
KpnI G_GTAC'C
Cuts at: 622 622
Size: 3602
MluI A'CGCG_T
Cuts at: 3035 3035
Size: 3602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2273 2457 2273
Size: 184 3418
MscI TGG'CCA
Cuts at: 112 1363 112
Size: 1251 2351
MslI CAynn'nnrTG
Cuts at: 1591 1718 1591
Size: 127 3475
MspA1I CmG'CkG
Cuts at: 416 713 1027 1387 2400 2645 416
Size: 297 314 360 1013 245 1373
Fragments arranged by size:
1373 1013 360 314 297 245
NaeI GCC'GGC
Cuts at: 1783 1783
Size: 3602
NarI GG'CG_CC
Cuts at: 1033 1281 1033
Size: 248 3354
NcoI C'CATG_G
Cuts at: 107 107
Size: 3602
NgoMIV G'CCGG_C
Cuts at: 1781 1781
Size: 3602
NspI r_CATG'y
Cuts at: 655 1155 2062 655
Size: 500 907 2195
Fragments arranged by size:
2195 907 500
PciI A'CATG_T
Cuts at: 2058 2058
Size: 3602
PfoI T'CCnGG_A
Cuts at: 564 564
Size: 3602
PmeI GTTT'AAAC
Cuts at: 601 601
Size: 3602
PmlI CAC'GTG
Cuts at: 530 530
Size: 3602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 3570
PstI C_TGCA'G
Cuts at: 189 365 649 1334 189
Size: 176 284 685 2457
Fragments arranged by size:
2457 685 284 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3602
PvuII CAG'CTG
Cuts at: 416 1027 1387 416
Size: 611 360 2631
Fragments arranged by size:
2631 611 360
RsrII CG'GwC_CG
Cuts at: 1797 1797
Size: 3602
SacI G_AGCT'C
Cuts at: 616 616
Size: 3602
SalI G'TCGA_C
Cuts at: 639 639
Size: 3602
SapI GCTCTTCn'nnn_
Cuts at: 1625 1835 1625
Size: 210 3392
SbfI CC_TGCA'GG
Cuts at: 649 649
Size: 3602
ScaI AGT'ACT
Cuts at: 3119 3119
Size: 3602
SfcI C'TryA_G
Cuts at: 33 185 361 645 1330 2323 2514 33
Size: 152 176 284 685 993 191 1121
Fragments arranged by size:
1121 993 685 284 191 176 152
SfoI GGC'GCC
Cuts at: 1034 1282 1034
Size: 248 3354
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3602
SmaI CCC'GGG
Cuts at: 624 624
Size: 3602
SmlI C'TyrA_G
Cuts at: 757 1672 2164 2426 757
Size: 915 492 262 1933
Fragments arranged by size:
1933 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3602
SphI G_CATG'C
Cuts at: 655 1155 655
Size: 500 3102
StyI C'CwwG_G
Cuts at: 107 119 736 1090 107
Size: 12 617 354 2619
Fragments arranged by size:
2619 617 354 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1185 1318 1777 1986 1185
Size: 133 459 209 2801
Fragments arranged by size:
2801 459 209 133
TatI w'GTAC_w
Cuts at: 3117 3117
Size: 3602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 3570
Tth111I GACn'n_nGTC
Cuts at: 1399 1399
Size: 3602
XbaI T'CTAG_A
Cuts at: 633 633
Size: 3602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1097 1097
Size: 3602
XmaI C'CCGG_G
Cuts at: 622 622
Size: 3602
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI